Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,288,032 C→A 5.6% intergenic (+22/‑58) yraH → / → yraI putative fimbrial‑like adhesin protein/putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,288,0320CA 5.6% 358.3 / 7.2 122intergenic (+22/‑58)yraH/yraIputative fimbrial‑like adhesin protein/putative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base C (43/72);  new base A (3/4);  total (46/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

Only 100 of 125 total aligned reads displayed.

TCCCGGCGGAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAAC  >  NC_000913/3287883‑3288181
                                                                                                                                                     |                                                                                                                                                     
tCCCGGCGGAATCCAATGGTTTTTTTATTGTTGACCTCATCGCTTACTACGAATGAAGCGTTGTTCCATCAGAAATCACCCCAGGGGAATCAGATGCTATCGGAAACGTACCGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGAcc                                                                                                                                                       <  1:1642728/150‑1 (MQ=255)
   cggcggAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGt                                                                                                                                                    >  2:515987/1‑150 (MQ=255)
             cAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTTTCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGATGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACGc                                                                                                                                          <  2:208396/150‑1 (MQ=255)
             cAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGCCCTGTCAGATAACGc                                                                                                                                          >  2:239376/1‑150 (MQ=255)
              aaTGGTATTGTCAATGTTGACCTCATCGCGTCCTACGTATCAACCGTAGTTCCATCAGAAATCTCCCCAGGGGAAGCAGACGCGATCGTTAACGTAACGCTGGATTACCGATACTACGTTAGGGCGTTATCTGACAGGGCAGATAACGcc                                                                                                                                         <  2:1004348/150‑1 (MQ=255)
               aTGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGccc                                                                                                                                        <  2:1656469/150‑1 (MQ=255)
               aTGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACGccc                                                                                                                                        <  2:1537/150‑1 (MQ=255)
               aTGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACGccc                                                                                                                                        <  1:170715/150‑1 (MQ=255)
                tGGTATTGTCAATGTTGACCTCATCGCTTACTACGTTACAACCGAAGTTCCATCACAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCt                                                                                                                                       <  2:1682272/150‑1 (MQ=255)
                 ggTATTGTCAATGTTGACCTCATCGCTTACTACGTATCATCCGTAGTTCCATCAGAAATCACCCCATGGGAAGCAGACGCTATCGTTAACGTACCGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGGCAGATAAGGCCCtt                                                                                                                                      >  2:203177/1‑150 (MQ=255)
                  gtcttGGCAATGCTCACCTCATCGCTTACTACGTATCAACCGACTTACCATCACAAATAACCCCCGGCGCCCCAGACGCTAGCGTTAACGTCACGCTCGAATACCGATAATACGTTAGGGCGTAATCAGACCTCTCAGATAACGCCCtta                                                                                                                                     <  2:263663/147‑2 (MQ=255)
                     ttGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttcc                                                                                                                                  <  1:449408/150‑1 (MQ=255)
                       gTCAATGTTGACCTCATCGCTTACTCCGTATCAACCGTAGTTCCATCAGAAATCACACCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttcctt                                                                                                                                <  2:1000279/150‑1 (MQ=255)
                           aTGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCctc                                                                                                                            <  1:1751481/150‑1 (MQ=255)
                            tGTTGACCTCATCGCTTACTCCGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCctct                                                                                                                           <  1:679086/150‑1 (MQ=255)
                             gTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCtt                                                                                                                          <  1:231207/150‑1 (MQ=255)
                               tGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTtc                                                                                                                        >  2:96497/1‑150 (MQ=255)
                               tGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTtc                                                                                                                        <  1:1603528/150‑1 (MQ=255)
                                gcccTCATCGCATACTACGTATCAACCGTAGTTCCATCAGAAAGCACCCCAGCGGAAGCAGACGCTATCATTAACGTAACGCTGGATTACCGTTAAAACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTtct                                                                                                                       <  2:346183/148‑1 (MQ=255)
                                 aCCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTTATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTtctc                                                                                                                      >  2:1795614/1‑150 (MQ=255)
                                    tCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGtt                                                                                                                   >  1:1459057/1‑150 (MQ=255)
                                    tCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGtt                                                                                                                   <  2:701185/150‑1 (MQ=255)
                                      aTCGCTTACTTCGTATCAACCGGAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATTCGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCGTTTCCGTCCGGTTTCTCGTTGt                                                                                                                 >  2:525894/1‑150 (MQ=255)
                                       tCGCTTACTACGTATCATCCGTAGTTCCATCTGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTa                                                                                                                <  1:398612/150‑1 (MQ=255)
                                       tCGCTTACTACGTATCAACCGTAGTTCCATCAGAACTCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTGTCTCGTTGtt                                                                                                                >  1:748883/1‑149 (MQ=255)
                                       tCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTa                                                                                                                >  1:1485774/1‑150 (MQ=255)
                                          cTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTTTCa                                                                                                             >  2:807234/1‑150 (MQ=255)
                                            tactacGTATCTACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTAAATACGTTTGGGCGTTATCTGACCTGTCATATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAgg                                                                                                           <  1:1539754/150‑1 (MQ=255)
                                             actacGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTCTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGt                                                                                                          <  2:1763916/150‑1 (MQ=255)
                                              atacGTATCAACCGTAGTTCCATCAGAAATCACCCCTGGGGAAGCAGACGCTATCGTAAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGtt                                                                                                         <  2:873388/149‑1 (MQ=255)
                                               tacGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTg                                                                                                        <  2:729182/150‑1 (MQ=255)
                                               tacGTATCAACCGTAGTTCCATCACAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCACATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTg                                                                                                        <  2:624826/150‑1 (MQ=255)
                                                 cGTATCAACCGTAGTTCCATCAGAAATCACCCCTGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGaa                                                                                                      <  2:1774099/150‑1 (MQ=255)
                                                  gTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGaaa                                                                                                     <  2:1508837/150‑1 (MQ=255)
                                                  gTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGaaa                                                                                                     <  1:1611061/150‑1 (MQ=255)
                                                    aTCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATATGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGaaaaa                                                                                                   >  2:1550607/1‑150 (MQ=255)
                                                       aaCCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATaa                                                                                                >  1:1324551/1‑150 (MQ=255)
                                                        aCCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAAc                                                                                               <  2:237534/150‑1 (MQ=255)
                                                        aCCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTGCCTCATTCTCGATGTATCAGCTTGAAAAATAAc                                                                                               >  1:293073/1‑150 (MQ=255)
                                                          cGTAGTTCCATCAGAAACCACCCCAGGCGAAGCAGACGCTATCCTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTa                                                                                             <  2:146352/150‑1 (MQ=255)
                                                            tAGTACCATCAGAAATCACCCCAGCGGAAGCAGACGCTTTCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCACTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATg                                                                                           <  1:1071284/150‑1 (MQ=255)
                                                              tttCCATCAGAAACCACCCCAGGGGAAGCTGACGCTATCGTTAACGTAACGCTTGATTACCTTTAATACGTTAGGGCGTTATCTGACCTGTCATATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTc                                                                                         <  1:1421216/149‑1 (MQ=255)
                                                               ttCCATCAGAAATCACCCCAGCGGAAGCAGACGCTATCGTTAACGTAACGCTGGATGACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATggcc                                                                                        <  1:514749/150‑4 (MQ=255)
                                                                 ccATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTGTCTCGTTGTATCAGGTTGAAAAATAACTATGGCaaa                                                                                      >  1:107476/1‑150 (MQ=255)
                                                                  cATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCaaaa                                                                                     >  1:1207910/1‑150 (MQ=255)
                                                                   aTCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAAc                                                                                    >  1:566580/1‑150 (MQ=255)
                                                                     cAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGa                                                                                  >  1:317512/1‑150 (MQ=255)
                                                                     cAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGCCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTTTCAGGTTGAAAAATAACTATGTCAAAACGa                                                                                  >  1:998140/1‑150 (MQ=255)
                                                                       taaaTCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAAc                                                                                <  1:151179/149‑1 (MQ=255)
                                                                       gAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAAc                                                                                <  1:1723700/150‑1 (MQ=255)
                                                                            cACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTcg                                                                           <  2:935613/150‑1 (MQ=255)
                                                                             aCCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTcgc                                                                          <  1:498600/150‑1 (MQ=255)
                                                                              ccccAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTcgcg                                                                         >  1:8412/1‑150 (MQ=255)
                                                                                ccAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGt                                                                       <  1:515987/150‑1 (MQ=255)
                                                                                 cAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTg                                                                      <  1:1271004/150‑1 (MQ=255)
                                                                                  aGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGa                                                                     <  2:1459057/150‑1 (MQ=255)
                                                                                  aGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCGCTTTCTCGGTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTgt                                                                     >  2:561411/1‑149 (MQ=255)
                                                                                          cAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAAcc                                                             >  2:1291904/1‑150 (MQ=255)
                                                                                             aCGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCttg                                                          <  1:409912/150‑1 (MQ=255)
                                                                                                cTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCttgttg                                                       <  1:1830584/150‑1 (MQ=255)
                                                                                                cTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCttgttg                                                       <  1:30276/150‑1 (MQ=255)
                                                                                                 tATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTACTCTTTATAGTTGTATCAGGTTGAAAAATAACTATGTAAAAACGAACATTCGCGGTGATATTTACCTtgttgt                                                      >  1:1615429/1‑150 (MQ=255)
                                                                                                  aTCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTtgtg                                                     <  2:877569/150‑1 (MQ=255)
                                                                                                  aTCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTtgtg                                                     <  2:193573/150‑1 (MQ=255)
                                                                                                   tCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTgtgt                                                    <  1:670190/150‑1 (MQ=255)
                                                                                                   tCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTa                                                   <  2:214481/150‑1 (MQ=255)
                                                                                                    cGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTa                                                   <  2:1110384/150‑1 (MQ=255)
                                                                                                      ttAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGc                                                 <  1:467124/150‑1 (MQ=255)
                                                                                                      ttAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGc                                                 <  2:317512/150‑1 (MQ=255)
                                                                                                       taacgtaacgCTGGATTACCTTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCt                                                <  1:1354786/150‑1 (MQ=255)
                                                                                                         acgtaacgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCATATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTc                                              <  1:1847170/150‑1 (MQ=255)
                                                                                                          cgtaacgCTGGAGTACCGATAATACGTTAGGGCGTTAGCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTGGAAAAATAACTATGTCAAAACGTACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCt                                             <  2:27972/150‑1 (MQ=255)
                                                                                                           gtaacgCTGGATTACCGTTAATACGTTAGGGCGTTATATGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTg                                            <  2:1163806/150‑1 (MQ=255)
                                                                                                               atcTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCATAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTAtt                                        <  1:1277820/148‑1 (MQ=255)
                                                                                                                gCTGTATAACCGTTAATACGTTAGGTCGTTATCTGACCTGTCAGATAACGCACTTTTCCTTCCTCTTTCTCGTTGTATCAGGCTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTg                                       <  2:155648/150‑1 (MQ=255)
                                                                                                                gCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTa                                                   <  2:1172733/138‑1 (MQ=255)
                                                                                                                gCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTa                                                   >  1:1172733/1‑138 (MQ=255)
                                                                                                                gCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTg                                       <  1:1291904/150‑1 (MQ=255)
                                                                                                                  tGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGc                                     <  2:1564325/150‑1 (MQ=255)
                                                                                                                      ttACCGTTTATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAgg                                 <  1:1795614/150‑1 (MQ=255)
                                                                                                                        aCCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGgcg                               >  2:208658/1‑150 (MQ=255)
                                                                                                                        aCCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTAGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGgcg                               >  2:343216/1‑150 (MQ=255)
                                                                                                                             tAATACGTTAGGGCGTTATCTGACCTGTCAGATTACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTTGCTTCTGTATTGGCCAGGCGCTTgg                          >  1:378401/1‑149 (MQ=255)
                                                                                                                             aaaTACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGc                          <  1:743698/149‑1 (MQ=255)
                                                                                                                                tACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCagg                       <  2:1762306/150‑1 (MQ=255)
                                                                                                                                tACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCagg                       <  2:1485774/150‑1 (MQ=255)
                                                                                                                                  cGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTACTTCCTCTTTCTCGTTGTATCAAGTTGAAAAATAACTATGTCAAAACGAACATTCGCCGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCaggag                     >  1:572423/1‑150 (MQ=255)
                                                                                                                                  cGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTGTTCCTACCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTCTAGCTTCTGTATTGGCCAGGCGCTTGCaggag                     >  1:303454/1‑150 (MQ=255)
                                                                                                                                         gCGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCTGGAGGAATCGt              >  1:175721/1‑150 (MQ=255)
                                                                                                                                          cGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGtt             <  1:344621/150‑1 (MQ=255)
                                                                                                                                          cGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCATGCGCTTGCAGGAGGAATCGtt             >  2:1378784/1‑150 (MQ=255)
                                                                                                                                          cGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCACGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAAAATTCGCTGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGtt             >  2:1632495/1‑150 (MQ=255)
                                                                                                                                           gTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTGGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGttt            <  2:1149129/150‑1 (MQ=255)
                                                                                                                                             tATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTa          >  1:1416360/1‑150 (MQ=255)
                                                                                                                                              aTCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTAc         >  2:1715729/1‑150 (MQ=255)
                                                                                                                                                cTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTGAAAACGATCATTCGCGGTGATATTATCCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAg       >  2:1231031/1‑150 (MQ=255)
                                                                                                                                                 tGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGc      <  1:1300221/150‑1 (MQ=255)
                                                                                                                                                  gACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACcgcg     >  2:1327734/1‑150 (MQ=255)
                                                                                                                                                    ccTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGaa   >  1:1815104/1‑150 (MQ=255)
                                                                                                                                                     cTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAAc  >  2:559100/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCCCGGCGGAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAAC  >  NC_000913/3287883‑3288181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: