Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,291,109 A→T 5.7% K766* (AAA→TAA)  yraJ → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,291,1090AT 5.7% 366.0 / 5.0 133K766* (AAA→TAA) yraJputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (66/58);  new base T (3/5);  total (70/63)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

TCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAATGT  >  NC_000913/3290960‑3291258
                                                                                                                                                     |                                                                                                                                                     
tctcGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCAAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGCTa                                                                                                                                                       >  1:311947/1‑150 (MQ=255)
 ctcGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTaa                                                                                                                                                      >  1:563233/1‑150 (MQ=255)
  gcGGTAGCTCCTTCGCAGCTGCCCGATACCGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCACCAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTaaa                                                                                                                                                     <  2:83384/149‑1 (MQ=255)
    ggTAGATACTACGCAGCTGCACGAAAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGGAAAcc                                                                                                                                                   <  1:1155338/150‑1 (MQ=255)
     gTAGATACTACGCAGCTGCCCGATAACGGCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCg                                                                                                                                                  <  2:802725/150‑1 (MQ=255)
       aGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGtt                                                                                                                                                <  1:1304807/150‑1 (MQ=255)
        caTACTACGCAGCGGCCCGATAACGTCGATCTTGAACAAACCACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTAACAGTCAGCGATCGCAACGGTAAACCGTTg                                                                                                                                               <  2:110358/149‑1 (MQ=255)
         aTACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGc                                                                                                                                              <  2:1495067/150‑1 (MQ=255)
           actacGCAGCTGCCCGATAACGTCGACCGGGAACAAACCACACCGGTTGTGGTGCCCAACAGAGGCGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcga                                                                                                                                            <  1:1654304/150‑3 (MQ=255)
               cGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAATCCGTTGCCCTTTg                                                                                                                                        >  2:1824213/1‑150 (MQ=255)
                gCAGCTGCCCGAGAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCACCGCCAAGATCGGTTATCGCGTACTTGTTGCAGGCAGCGATCGCACCGGTAAACCGGTGCCCTGTgg                                                                                                                                       >  1:706133/1‑150 (MQ=255)
                  aGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGgcg                                                                                                                                     <  1:1020628/150‑1 (MQ=255)
                   gCTGACCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGgcgc                                                                                                                                    <  1:1575980/150‑1 (MQ=255)
                      gCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCACCGGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACGGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGctct                                                                                                                                 >  2:175109/1‑150 (MQ=255)
                        ccGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTg                                                                                                                               <  1:1769933/150‑1 (MQ=255)
                               cGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCac                                                                                                                        >  1:473525/1‑149 (MQ=255)
                                 tCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACg                                                                                                                      >  1:1209145/1‑150 (MQ=255)
                                   gATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgat                                                                                                                    <  2:1383945/150‑1 (MQ=255)
                                   gATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgat                                                                                                                    >  1:846260/1‑150 (MQ=255)
                                      cTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgatgat                                                                                                                 <  1:1277893/150‑1 (MQ=255)
                                      cTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgatgat                                                                                                                 >  1:376327/1‑150 (MQ=255)
                                       ttGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATa                                                                                                                >  2:169804/1‑150 (MQ=255)
                                         gAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACg                                                                                                              >  2:550036/1‑150 (MQ=255)
                                          aacaaacaACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTGAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTAACCGTTGCCCTTTGGCGCTGTTGGCAGCAACGATGATACgg                                                                                                             >  2:458439/1‑150 (MQ=255)
                                             aaacaaCACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGc                                                                                                          <  2:1597021/150‑1 (MQ=255)
                                             aaacaaCACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGc                                                                                                          <  1:151558/150‑1 (MQ=255)
                                                caacaCAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGGTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTGAGCGATCGCAACGGTAAGCCGTTGGCCTTTGGCGCTCTTGCCGGCTACGAGGATGCgggggacc                                                                                                       >  2:916732/1‑146 (MQ=255)
                                                   cacaGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCGAATATCGGTTATCGCGTACTTGTGACAGTCCGCGATCGCACCGGTAAGCCGTTGCCGTTTGGCGCTGGTGGCAGCAACGATGAGACGGGGCAACaaa                                                                                                    >  2:31354/1‑150 (MQ=255)
                                                   cacaGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACaaa                                                                                                    >  2:499650/1‑150 (MQ=255)
                                                    acaGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAg                                                                                                   <  1:1464018/150‑1 (MQ=255)
                                                       gTTTGTGGTGCCCAACAGAGGTGCAATTGTAGCGGCGCGTTGCAACGCCAATATCGGTTATCGCGTACTTGTTAGAGTCAGCGATCGCAACTGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAt                                                                                                <  1:96060/150‑1 (MQ=255)
                                                           gtggtgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAtcgtc                                                                                            >  1:1025827/1‑150 (MQ=255)
                                                           gtggtgCCCAACAGAGGGGCAATGGTAGCGGCGCGTTTCAACGCCAAAAGCGGTTATCGCGTACGGGGTACAGTCCGCGATCGCCACGGTGAACCGTTGACCTTGGGGGCTCTTGCGAGGCACGAGGATCCGGGGCCACAAAGTAtcggg                                                                                            >  2:904357/1‑148 (MQ=255)
                                                            tggggCCCACCAGAGGTGCAATGGTAGCGTCGCGTTTCAACGCCAATATCGGTTATAGCGTACCTGTTCCAGTCAGCGAGCGCCACGGTAAACCGTTGCCCGTTGGCGCGCTTGCCAGCAACGATGATACGGGGCAACAAAGTAtcgtcg                                                                                           <  1:999360/150‑1 (MQ=255)
                                                               tgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATCTCGGTTAGCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCTCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATg                                                                                        <  1:1718219/150‑1 (MQ=255)
                                                                gCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTATTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGa                                                                                       >  2:1803390/1‑150 (MQ=255)
                                                                   cAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAggg                                                                                    <  1:1842458/150‑1 (MQ=255)
                                                                     aCAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcg                                                                                  <  2:1025827/150‑1 (MQ=255)
                                                                          ggTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTAGTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATa                                                                             >  1:202909/1‑150 (MQ=255)
                                                                          ggTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCCATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCGAGGAACGATGATACGGGGCATCAAAGTTAGGTCGATGAGGGCGGCATa                                                                             >  2:263769/1‑150 (MQ=255)
                                                                           gTGCTATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACATAGTATCGTCGATGAGGGCGGCATAc                                                                            <  1:670850/150‑1 (MQ=255)
                                                                             gCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACta                                                                          <  1:738723/150‑1 (MQ=255)
                                                                             gCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACta                                                                          >  1:1408604/1‑150 (MQ=255)
                                                                              cAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtat                                                                         >  2:343958/1‑150 (MQ=255)
                                                                                 tGGTATCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGGGATCGCAACGGTAACCCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtc                                                                      <  2:778452/150‑1 (MQ=255)
                                                                                  ggTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtct                                                                     >  1:1378721/1‑150 (MQ=255)
                                                                                   gTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtctc                                                                    <  2:1522248/150‑1 (MQ=255)
                                                                                     aGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGCTCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtctctc                                                                  >  2:288929/1‑150 (MQ=255)
                                                                                      gcggcgCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTTCAAGCAACGATGATACGGGGGAACAAAGTTTCGTCGATGAGGGCGGCATACTATTtcgctgt                                                                 >  2:726732/1‑148 (MQ=255)
                                                                                       cggcgCGTTTCAACGCCAATCTCGGTTATCGCGTACTTGTTACACTCAGCGATCGCAACGGTAAACCGTTGCCCTGTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGAATCGTCGATGAGGGCGGCATACTATATCTCTCTg                                                                <  2:85317/150‑1 (MQ=255)
                                                                                        ggcgCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTgg                                                               >  2:1223657/1‑150 (MQ=255)
                                                                                        ggcgCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTgg                                                               <  2:1867177/150‑1 (MQ=255)
                                                                                          cgcgTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATGTCTCTGGGa                                                             >  2:406198/1‑150 (MQ=255)
                                                                                             gTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGatat                                                          >  2:1461447/1‑150 (MQ=255)
                                                                                               ttCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCg                                                        <  2:792261/150‑1 (MQ=255)
                                                                                                 cAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGCTACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAg                                                      >  2:1784976/1‑150 (MQ=255)
                                                                                                  aaCGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGt                                                     >  2:1481851/1‑150 (MQ=255)
                                                                                                   aCGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTa                                                    <  2:563233/150‑1 (MQ=255)
                                                                                                    cGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTaa                                                   >  2:1503715/1‑150 (MQ=255)
                                                                                                    cGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTaa                                                   >  2:1762459/1‑150 (MQ=255)
                                                                                                          aatCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATcaca                                             <  2:582741/149‑1 (MQ=255)
                                                                                                           atCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTGTTGCCCGCAACGATGATACGGGGCAACAAAGGATCGGGGCTGAGGGCGGCATACTATTTCTCTCTGGGATATCGAGTAAATCGCaa                                            >  2:1185048/1‑150 (MQ=255)
                                                                                                              ggTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAGCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGc                                         >  2:677225/1‑150 (MQ=255)
                                                                                                                   tCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGAc                                    >  2:1563390/1‑150 (MQ=255)
                                                                                                                   tCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGAc                                    >  1:607313/1‑150 (MQ=255)
                                                                                                                   tCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGAc                                    >  1:1106697/1‑150 (MQ=255)
                                                                                                                   tCGCGGACTTGTTACAGGCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGAc                                    <  2:1655629/150‑1 (MQ=255)
                                                                                                                   tCGAGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGAc                                    <  2:606615/150‑1 (MQ=255)
                                                                                                                   ccgcgAACTTCTAACACTCAGCCATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACCCGGCAACAAAGTATCGTCGATGAGGCCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGAc                                    <  1:1019995/149‑1 (MQ=255)
                                                                                                                    cgcgTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACt                                   <  1:552580/150‑1 (MQ=255)
                                                                                                                    cgcgTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACt                                   <  2:39610/150‑1 (MQ=255)
                                                                                                                      cgTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGt                                 >  2:560303/1‑150 (MQ=255)
                                                                                                                       gTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTa                                <  1:458439/150‑1 (MQ=255)
                                                                                                                        tACTTGTTACAGTCAGCGATCGCAACGGTAACCCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTAc                               <  2:1458342/150‑1 (MQ=255)
                                                                                                                             gTTACAGTCAGCGATCGCAATGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTgg                          >  1:1208786/1‑150 (MQ=255)
                                                                                                                              ttACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTggg                         <  2:1408604/150‑1 (MQ=255)
                                                                                                                               tACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTgggg                        <  2:1566678/150‑1 (MQ=255)
                                                                                                                                aCAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGa                       <  1:967554/150‑1 (MQ=255)
                                                                                                                                   gTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAAt                    >  1:1476165/1‑150 (MQ=255)
                                                                                                                                     cAGCGATCGCAACGGTAAACCTTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATAAGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTAAATCTCTCTGGGTTATCGAGTAAATAACAAATCTTGACTGTTCGCTGGTGAAAtaa                  >  2:661447/1‑148 (MQ=255)
                                                                                                                                      ccaaagggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAg                 >  1:1147673/8‑150 (MQ=255)
                                                                                                                                       gCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAgg                >  2:530695/1‑150 (MQ=255)
                                                                                                                                        cGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGTGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGc               >  2:1345630/1‑150 (MQ=255)
                                                                                                                                          aTCGCAACGCTTAACCGTTGCCGTTTGCCTCTCTTGCCAGCAACGATGATACGGGGCCACAAAGTATCGTCGATGAGGGCGGCTTACTATATCTCTCTGGGATATCGAGTAAATCACATAGCTGGACTGTACGCTGGGGAAATCAGGCAg             <  1:904357/150‑1 (MQ=255)
                                                                                                                                           tCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGTCAACAAATTATCGTAGATGAGGTCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGa            <  1:730173/150‑1 (MQ=255)
                                                                                                                                            ggCAACGGTTTACCGTTGCGCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGGAACAAAGTATCGTCGATGCGGGCGGCATACTATATCTCTCGGGGATATCGAGTAAATCCCAAAGCGGGACTGTCGGCTGGGGAGATCAGGgagag           >  2:212690/2‑149 (MQ=255)
                                                                                                                                            cGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGAt           >  1:304274/1‑150 (MQ=255)
                                                                                                                                              catcGGTAAACCGTTGCCCTTTGGCGATCTTGCCAGCAACTATGATACGGGGCAACTAAGTTTCGTCGATGAGGTCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATca         <  1:289696/147‑1 (MQ=255)
                                                                                                                                              cAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCACCAACGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATAGCGAGTAAATCACAAAGCTGGACTGGACGCTGGGGAAATCAGGCAGAtga         >  1:308780/1‑148 (MQ=255)
                                                                                                                                              cAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATca         >  1:1669202/1‑150 (MQ=255)
                                                                                                                                              cAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATca         >  1:1507569/1‑150 (MQ=255)
                                                                                                                                                 cGGTAAACCGTTGGCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATcaaca      <  2:846260/150‑1 (MQ=255)
                                                                                                                                                   gTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAAt    >  1:535012/1‑150 (MQ=255)
                                                                                                                                                    tAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAGCGATGATACGGGGCAACAAAGTATCGGCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACGGTACGCTGGGGAAATCAGGCAGATAAACAATg   >  2:1132875/1‑150 (MQ=255)
                                                                                                                                                    tAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGCAACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTATCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAAt  >  1:888746/1‑149 (MQ=255)
                                                                                                                                                    tAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAATg   >  2:707293/1‑150 (MQ=255)
                                                                                                                                                    tAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAATg   >  1:1733379/1‑150 (MQ=255)
                                                                                                                                                    tAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAATg   <  1:550036/150‑1 (MQ=255)
                                                                                                                                                    tAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAATg   >  1:40133/1‑150 (MQ=255)
                                                                                                                                                     aaaCCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAATGt  >  2:1874239/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAATGT  >  NC_000913/3290960‑3291258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: