Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,305,913 G→T 6.1% intergenic (‑96/+58) mtr ← / ← deaD tryptophan transporter of high affinity/ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,305,9130GT 6.1% 265.1 / 8.0 100intergenic (‑96/+58)mtr/deaDtryptophan transporter of high affinity/ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base G (49/44);  new base T (2/4);  total (51/49)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.68e-01

Only 100 of 103 total aligned reads displayed.

CACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCAT  >  NC_000913/3305764‑3306062
                                                                                                                                                      |                                                                                                                                                     
caccacGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATg                                                                                                                                                       >  2:879578/1‑150 (MQ=255)
 accacGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATgg                                                                                                                                                      >  2:1088668/1‑150 (MQ=255)
      gccgccAAGCAGCGACGGTGACGTTGGGGTGGTGGTTAGTGTTGCCCTGAGGGCTGCTCTCCAGTGAAAAATAGTGCGCCTGCGTTGTTATGCATTGCACTGTACCAGTACACTAGTACAACCGACAGAAAAAAAGCCCCGATGGaaaca                                                                                                                                                 >  1:319652/1‑148 (MQ=255)
           cAAGCAGCGACGGTGACGTTTGGGTGGTGGTATGGGTTGCCATGTGGTCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACATAAGACAGAAAATAAGCCCCGATGGTTAAAATCgg                                                                                                                                            <  2:633390/150‑1 (MQ=255)
              gcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGc                                                                                                                                         <  2:624126/150‑1 (MQ=255)
              gcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGc                                                                                                                                         <  2:1256566/150‑1 (MQ=255)
              gcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGc                                                                                                                                         >  1:1488611/1‑150 (MQ=255)
               cagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGCt                                                                                                                                        <  2:620965/150‑1 (MQ=255)
               cagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCAGATGGTAAAAATCGGGGCt                                                                                                                                        >  2:1239160/1‑150 (MQ=255)
                 gcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGCttt                                                                                                                                      <  2:1497796/150‑3 (MQ=255)
                  cGACGGTGACGTTTGGGTGGTGGTTAGTGTGGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGta                                                                                                                                     <  1:1847609/150‑1 (MQ=255)
                  cGACGGTGACGTTTGGGTGGTGGTTAGTGGTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAACATCGGGGCTGta                                                                                                                                     <  2:1592683/150‑1 (MQ=255)
                   gccGGTGACGTTTGGGTGGTGGGTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGGGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAACACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtat                                                                                                                                    <  1:830048/148‑1 (MQ=255)
                    aCGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtata                                                                                                                                   <  1:1025021/150‑1 (MQ=255)
                     cGGTGACGTTTGGGTGGTTGTTAGTGTTGCCATGAGGTCTTATCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGAACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtatat                                                                                                                                  <  1:123867/150‑1 (MQ=255)
                     cGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCTCTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtatat                                                                                                                                  >  2:552701/1‑150 (MQ=255)
                           cggttGGGGCGTGGTTAGTGTGGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGTGTTGTATATattatt                                                                                                                            <  2:364852/147‑1 (MQ=255)
                            gTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTAttt                                                                                                                           >  2:1204390/1‑150 (MQ=255)
                               tGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTAc                                                                                                                        <  1:1035203/150‑1 (MQ=255)
                                gggTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACa                                                                                                                       <  1:1624256/150‑1 (MQ=255)
                                 ggtggtggtTAGTGTTGCCATGAGGGCTTCTCACCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACTAGTACAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGa                                                                                                                     <  2:1194739/150‑1 (MQ=255)
                                  gtggtggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAATACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGa                                                                                                                     <  1:1271774/150‑1 (MQ=255)
                                  gagggggtTAGTGTTGCCATGAGGGCGTCTCTCCAGTGAAAAATAGTCCGACTGCGCTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCAGATGTTAAAAATCGGGGCTGTATATATTATTTTACAGa                                                                                                                     <  2:1655283/145‑1 (MQ=255)
                                     gtggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtg                                                                                                                  <  1:401137/150‑1 (MQ=255)
                                     gtggtAAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtg                                                                                                                  <  2:336385/150‑1 (MQ=255)
                                        gtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTgtgt                                                                                                               >  2:1430306/1‑150 (MQ=255)
                                           aGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCg                                                                                                            <  1:419377/150‑1 (MQ=255)
                                            gtgtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGc                                                                                                           <  2:1376228/150‑1 (MQ=255)
                                            gtgtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGAGACTGCGTTGTTATACATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGc                                                                                                           <  1:70990/150‑1 (MQ=255)
                                              gtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGGTGTTATGCATTGCACTGTACCTGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTg                                                                                                         <  2:1513589/150‑1 (MQ=255)
                                               ttGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGt                                                                                                        <  2:1754603/150‑1 (MQ=255)
                                                tGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGtt                                                                                                       <  2:381631/150‑1 (MQ=255)
                                                 gCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGtt                                                                                                       >  1:1328195/1‑150 (MQ=255)
                                                  ccATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCa                                                                                                     <  1:576333/150‑1 (MQ=255)
                                                    aTGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGc                                                                                                   <  1:214244/150‑1 (MQ=255)
                                                       tgggCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgat                                                                                                <  2:207653/149‑1 (MQ=255)
                                                        gggCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCTATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgatg                                                                                               <  2:41432/150‑1 (MQ=255)
                                                         ggCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgatga                                                                                              >  1:889268/1‑150 (MQ=255)
                                                                ctcCAGTTAAAAATAGTGCGACTGCGTTGTTATGCATTTCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCa                                                                                       <  1:372923/150‑1 (MQ=255)
                                                                ctcCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCa                                                                                       <  1:632361/150‑1 (MQ=255)
                                                                  ccAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATc                                                                                     >  1:1391197/1‑150 (MQ=255)
                                                                     gTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcacc                                                                                  >  1:1576505/1‑150 (MQ=255)
                                                                     gTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcacc                                                                                  >  2:1239031/1‑150 (MQ=255)
                                                                     gTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcacc                                                                                  >  2:805299/1‑150 (MQ=255)
                                                                       gAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcaccac                                                                                >  2:12142/1‑150 (MQ=255)
                                                                        aaaaaTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCaccacc                                                                               <  1:879578/150‑1 (MQ=255)
                                                                         aaaaTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCg                                                                              >  2:1737877/1‑150 (MQ=255)
                                                                              aGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacg                                                                         <  2:836947/150‑1 (MQ=255)
                                                                              aGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacg                                                                         >  2:1700427/1‑150 (MQ=255)
                                                                              aGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacg                                                                         >  2:439762/1‑150 (MQ=255)
                                                                               gTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacga                                                                        >  2:331556/1‑150 (MQ=255)
                                                                                 ccGACTGCGTTGTTATGCATTGCACTGGACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacg                                                                      <  2:1369090/149‑1 (MQ=255)
                                                                                   taCTGCGTTGTAATGCATTGCACTGTACCAGTACACTAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacgac                                                                    <  2:436474/149‑1 (MQ=255)
                                                                                   gACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacgac                                                                    >  2:1123007/1‑150 (MQ=255)
                                                                                   gACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGCGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacgac                                                                    >  1:178431/1‑150 (MQ=255)
                                                                                    aCTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAGGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacgacg                                                                   <  1:827373/150‑1 (MQ=255)
                                                                                         gttgttATGCATTGCACTGTACACGAACACGAGTACAAAAGACAGAAAAAACGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTGCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCgg                                                              <  2:1690519/150‑1 (MQ=255)
                                                                                          ttgttATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGt                                                             <  2:1851535/150‑1 (MQ=255)
                                                                                           tgttATGCATTTCACTGTACCAGAACACGAGTACAAAAGACATAAAAAAAGCCCCGATGTTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTa                                                            <  2:1666930/150‑1 (MQ=255)
                                                                                            gttATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAg                                                           >  1:948395/1‑150 (MQ=255)
                                                                                            gttATGCATTGCACTGTACCAGTACACGAGTACAAAAGACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAg                                                           <  2:1345943/150‑1 (MQ=255)
                                                                                              tATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTAATTTAAAGATTGTTTTCTCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGAAGCCCGGTAGaa                                                         >  2:292488/1‑150 (MQ=255)
                                                                                               aTGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAat                                                        <  1:1232216/150‑1 (MQ=255)
                                                                                                 gCATTGCACTGTACCTCTACACGAGAACAACAGACACAAAAACAGCCCCGACCGGAAAAATCGGGGCTGTATATATTATTTAACAGATTGTCTTCGCTGTTCAGCGATGATTACGCATCACCCCCGAAACGACGCCGACCGGTAGAatca                                                      <  2:699897/150‑1 (MQ=255)
                                                                                                 gCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGGAGAatca                                                      >  2:304228/1‑150 (MQ=255)
                                                                                                   aTTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatcctc                                                    >  2:262313/1‑150 (MQ=255)
                                                                                                   aTTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatcatc                                                    >  1:798407/1‑150 (MQ=255)
                                                                                                         cTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCacgacg                                              >  2:601955/1‑150 (MQ=255)
                                                                                                           gTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGAGGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGAcgcg                                            >  2:1753975/1‑150 (MQ=255)
                                                                                                            tACCAGTACATGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCAACACCGATACGACGACGACCGGTAGAATCATCACGACGCgg                                           >  1:400567/1‑150 (MQ=255)
                                                                                                            tACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCgg                                           >  1:42467/1‑150 (MQ=255)
                                                                                                              ccAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAg                                         >  1:1065353/1‑150 (MQ=255)
                                                                                                                   acacCAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCgg                                    <  2:1782196/150‑1 (MQ=255)
                                                                                                                    cacGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGc                                   <  1:1263117/150‑1 (MQ=255)
                                                                                                                    cacGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGAAGAATCATCACGACCCGGAGCGCGGc                                   >  2:1162897/1‑150 (MQ=255)
                                                                                                                      cGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGCCGACCGGTAGAATCATCACGACGCGGAGCGCGGCCt                                 >  1:375256/1‑150 (MQ=255)
                                                                                                                          tACAAAAGCCAGAAAAAAAGCCCCTATGGTAATATTCGGGGCTGTATATATTATTTTACATATTGTGTTGTATGTTCAGCGTTGATTACGAACCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCac                             <  1:1430306/150‑1 (MQ=255)
                                                                                                                           aCAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGCCCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCacg                            >  2:449437/1‑150 (MQ=255)
                                                                                                                           aCAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCacg                            >  1:439544/1‑150 (MQ=255)
                                                                                                                            cAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGCCGACCGCTAGAATCATCACGACGCGGAGCGCGGCCTTCacgc                           >  1:1036632/1‑149 (MQ=255)
                                                                                                                            cAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCacga                           >  2:1116251/1‑150 (MQ=255)
                                                                                                                            cAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCacga                           >  2:870587/1‑150 (MQ=255)
                                                                                                                              aaaGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTACacgacg                         >  1:705286/1‑150 (MQ=255)
                                                                                                                               aaGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGCCGt                        >  1:998727/1‑150 (MQ=255)
                                                                                                                                   cAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgc                    >  2:768445/1‑150 (MQ=255)
                                                                                                                                   cAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTAACCACGTTcgc                    >  1:800772/1‑150 (MQ=255)
                                                                                                                                   cAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTCTATAAATTATATTACAGATTGTGTTCGCTGATCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGAAGCATCATCACGACGAGGAGCGCGGCCTTCACGACGTTcgc                    >  1:2230/1‑150 (MQ=255)
                                                                                                                                    aGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgcc                   <  1:1737877/150‑1 (MQ=255)
                                                                                                                                     gAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACTGTTTGTGTTCGCTGTTAAGCGATGATTACGCATCACCCCCGCAACGACGACGACCGGTCGCATCATCACGACGCGGAGCGCGGCCTTCACGACGTTctccg                  >  2:260368/1‑150 (MQ=255)
                                                                                                                                     gAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGCCGCGGAGCGCGGCCTTCACGACGTTcgccg                  >  2:136802/1‑150 (MQ=255)
                                                                                                                                     gAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgccg                  >  1:1072547/1‑150 (MQ=255)
                                                                                                                                        aaaaaaGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCCCCACCGACCCGACGACGCCCGGTAGAATCTTCACGCCGCGGCGCGCGGCGTTCCCGCCGTTCGCCGCt                >  1:1009214/1‑150 (MQ=255)
                                                                                                                                        aaaaaaGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCt                >  2:1825701/1‑150 (MQ=255)
                                                                                                                                             aGCCCCGATTTTTACCATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCTGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCGAAAac           >  1:172474/1‑150 (MQ=255)
                                                                                                                                             aGCCCCGATTTTTACCATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCACTAAAac           >  1:765913/1‑150 (MQ=255)
                                                                                                                                              gCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAacg          >  2:162363/1‑150 (MQ=255)
                                                                                                                                              gCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAacg          <  1:552701/150‑1 (MQ=255)
                                                                                                                                              gCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTACAATAAACACCACGCGGAGCACGGCATTCACGACCTTCGCACCAAAAacc          >  2:1208414/1‑149 (MQ=255)
                                                                                                                                                  cGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAAcgacga      <  1:1239031/150‑1 (MQ=255)
                                                                                                                                                      ggTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCAt  >  2:416779/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCAT  >  NC_000913/3305764‑3306062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: