Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,305,914 G→T 7.2% intergenic (‑97/+57) mtr ← / ← deaD tryptophan transporter of high affinity/ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,305,9140GT 7.2% 287.0 / 8.8 100intergenic (‑97/+57)mtr/deaDtryptophan transporter of high affinity/ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base G (49/43);  new base T (2/6);  total (51/49)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 102 total aligned reads displayed.

ACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCAT  >  NC_000913/3305765‑3306062
                                                                                                                                                      |                                                                                                                                                    
accacGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATgg                                                                                                                                                      >  2:1088668/1‑150 (MQ=255)
     gccgccAAGCAGCGACGGTGACGTTGGGGTGGTGGTTAGTGTTGCCCTGAGGGCTGCTCTCCAGTGAAAAATAGTGCGCCTGCGTTGTTATGCATTGCACTGTACCAGTACACTAGTACAACCGACAGAAAAAAAGCCCCGATGGaaaca                                                                                                                                                 >  1:319652/1‑148 (MQ=255)
          cAAGCAGCGACGGTGACGTTTGGGTGGTGGTATGGGTTGCCATGTGGTCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACATAAGACAGAAAATAAGCCCCGATGGTTAAAATCgg                                                                                                                                            <  2:633390/150‑1 (MQ=255)
             gcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGc                                                                                                                                         <  2:624126/150‑1 (MQ=255)
             gcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGc                                                                                                                                         >  1:1488611/1‑150 (MQ=255)
             gcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGc                                                                                                                                         <  2:1256566/150‑1 (MQ=255)
              cagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGCt                                                                                                                                        <  2:620965/150‑1 (MQ=255)
              cagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCAGATGGTAAAAATCGGGGCt                                                                                                                                        >  2:1239160/1‑150 (MQ=255)
                gcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGCttt                                                                                                                                      <  2:1497796/150‑3 (MQ=255)
                 cGACGGTGACGTTTGGGTGGTGGTTAGTGTGGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGta                                                                                                                                     <  1:1847609/150‑1 (MQ=255)
                 cGACGGTGACGTTTGGGTGGTGGTTAGTGGTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAACATCGGGGCTGta                                                                                                                                     <  2:1592683/150‑1 (MQ=255)
                  gccGGTGACGTTTGGGTGGTGGGTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGGGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAACACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtat                                                                                                                                    <  1:830048/148‑1 (MQ=255)
                   aCGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtata                                                                                                                                   <  1:1025021/150‑1 (MQ=255)
                    cGGTGACGTTTGGGTGGTTGTTAGTGTTGCCATGAGGTCTTATCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGAACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtatat                                                                                                                                  <  1:123867/150‑1 (MQ=255)
                    cGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCTCTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtatat                                                                                                                                  >  2:552701/1‑150 (MQ=255)
                          cggttGGGGCGTGGTTAGTGTGGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGTGTTGTATATattatt                                                                                                                            <  2:364852/147‑1 (MQ=255)
                           gTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTAttt                                                                                                                           >  2:1204390/1‑150 (MQ=255)
                              tGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTAc                                                                                                                        <  1:1035203/150‑1 (MQ=255)
                               gggTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACa                                                                                                                       <  1:1624256/150‑1 (MQ=255)
                                 gtggtggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAATACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGa                                                                                                                     <  1:1271774/150‑1 (MQ=255)
                                 gagggggtTAGTGTTGCCATGAGGGCGTCTCTCCAGTGAAAAATAGTCCGACTGCGCTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCAGATGTTAAAAATCGGGGCTGTATATATTATTTTACAGa                                                                                                                     <  2:1655283/145‑1 (MQ=255)
                                    gtggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtg                                                                                                                  <  1:401137/150‑1 (MQ=255)
                                    gtggtAAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtg                                                                                                                  <  2:336385/150‑1 (MQ=255)
                                       gtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTgtgt                                                                                                               >  2:1430306/1‑150 (MQ=255)
                                          aGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCg                                                                                                            <  1:419377/150‑1 (MQ=255)
                                           gtgtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGc                                                                                                           <  2:1376228/150‑1 (MQ=255)
                                           gtgtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGAGACTGCGTTGTTATACATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGc                                                                                                           <  1:70990/150‑1 (MQ=255)
                                             gtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGGTGTTATGCATTGCACTGTACCTGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTg                                                                                                         <  2:1513589/150‑1 (MQ=255)
                                              ttGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGt                                                                                                        <  2:1754603/150‑1 (MQ=255)
                                               tGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGtt                                                                                                       <  2:381631/150‑1 (MQ=255)
                                                gCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGtt                                                                                                       >  1:1328195/1‑150 (MQ=255)
                                                 ccATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCa                                                                                                     <  1:576333/150‑1 (MQ=255)
                                                   aTGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGc                                                                                                   <  1:214244/150‑1 (MQ=255)
                                                      tgggCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgat                                                                                                <  2:207653/149‑1 (MQ=255)
                                                       gggCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCTATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgatg                                                                                               <  2:41432/150‑1 (MQ=255)
                                                        ggCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgatga                                                                                              >  1:889268/1‑150 (MQ=255)
                                                               ctcCAGTTAAAAATAGTGCGACTGCGTTGTTATGCATTTCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCa                                                                                       <  1:372923/150‑1 (MQ=255)
                                                               ctcCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCa                                                                                       <  1:632361/150‑1 (MQ=255)
                                                                 ccAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATc                                                                                     >  1:1391197/1‑150 (MQ=255)
                                                                    gTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcacc                                                                                  >  2:1239031/1‑150 (MQ=255)
                                                                    gTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcacc                                                                                  >  1:1576505/1‑150 (MQ=255)
                                                                    gTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcacc                                                                                  >  2:805299/1‑150 (MQ=255)
                                                                      gAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcaccac                                                                                >  2:12142/1‑150 (MQ=255)
                                                                       aaaaaTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCaccacc                                                                               <  1:879578/150‑1 (MQ=255)
                                                                        aaaaTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCg                                                                              >  2:1737877/1‑150 (MQ=255)
                                                                             aGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacg                                                                         >  2:1700427/1‑150 (MQ=255)
                                                                             aGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacg                                                                         >  2:439762/1‑150 (MQ=255)
                                                                             aGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacg                                                                         <  2:836947/150‑1 (MQ=255)
                                                                              gTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacga                                                                        >  2:331556/1‑150 (MQ=255)
                                                                                ccGACTGCGTTGTTATGCATTGCACTGGACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacg                                                                      <  2:1369090/149‑1 (MQ=255)
                                                                                  taCTGCGTTGTAATGCATTGCACTGTACCAGTACACTAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacgac                                                                    <  2:436474/149‑1 (MQ=255)
                                                                                  gACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacgac                                                                    >  2:1123007/1‑150 (MQ=255)
                                                                                  gACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGCGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacgac                                                                    >  1:178431/1‑150 (MQ=255)
                                                                                   aCTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAGGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacgacg                                                                   <  1:827373/150‑1 (MQ=255)
                                                                                        gttgttATGCATTGCACTGTACACGAACACGAGTACAAAAGACAGAAAAAACGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTGCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCgg                                                              <  2:1690519/150‑1 (MQ=255)
                                                                                         ttgttATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGt                                                             <  2:1851535/150‑1 (MQ=255)
                                                                                          tgttATGCATTTCACTGTACCAGAACACGAGTACAAAAGACATAAAAAAAGCCCCGATGTTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTa                                                            <  2:1666930/150‑1 (MQ=255)
                                                                                           gttATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAg                                                           >  1:948395/1‑150 (MQ=255)
                                                                                           gttATGCATTGCACTGTACCAGTACACGAGTACAAAAGACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAg                                                           <  2:1345943/150‑1 (MQ=255)
                                                                                             tATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTAATTTAAAGATTGTTTTCTCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGAAGCCCGGTAGaa                                                         >  2:292488/1‑150 (MQ=255)
                                                                                              aTGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAat                                                        <  1:1232216/150‑1 (MQ=255)
                                                                                               tGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatc                                                       <  2:1670894/150‑1 (MQ=255)
                                                                                                gCATTGCACTGTACCTCTACACGAGAACAACAGACACAAAAACAGCCCCGACCGGAAAAATCGGGGCTGTATATATTATTTAACAGATTGTCTTCGCTGTTCAGCGATGATTACGCATCACCCCCGAAACGACGCCGACCGGTAGAatca                                                      <  2:699897/150‑1 (MQ=255)
                                                                                                gCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGGAGAatca                                                      >  2:304228/1‑150 (MQ=255)
                                                                                                  aTTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatcctc                                                    >  2:262313/1‑150 (MQ=255)
                                                                                                  aTTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatcatc                                                    >  1:798407/1‑150 (MQ=255)
                                                                                                        cTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCacgacg                                              >  2:601955/1‑150 (MQ=255)
                                                                                                          gTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGAGGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGAcgcg                                            >  2:1753975/1‑150 (MQ=255)
                                                                                                           tACCAGTACATGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCAACACCGATACGACGACGACCGGTAGAATCATCACGACGCgg                                           >  1:400567/1‑150 (MQ=255)
                                                                                                           tACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCgg                                           >  1:42467/1‑150 (MQ=255)
                                                                                                             ccAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAg                                         >  1:1065353/1‑150 (MQ=255)
                                                                                                                  acacCAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCgg                                    <  2:1782196/150‑1 (MQ=255)
                                                                                                                   cacGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGc                                   <  1:1263117/150‑1 (MQ=255)
                                                                                                                   cacGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGAAGAATCATCACGACCCGGAGCGCGGc                                   >  2:1162897/1‑150 (MQ=255)
                                                                                                                     cGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGCCGACCGGTAGAATCATCACGACGCGGAGCGCGGCCt                                 >  1:375256/1‑150 (MQ=255)
                                                                                                                         tACAAAAGCCAGAAAAAAAGCCCCTATGGTAATATTCGGGGCTGTATATATTATTTTACATATTGTGTTGTATGTTCAGCGTTGATTACGAACCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCac                             <  1:1430306/150‑1 (MQ=255)
                                                                                                                          aCAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGCCCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCacg                            >  2:449437/1‑150 (MQ=255)
                                                                                                                          aCAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCacg                            >  1:439544/1‑150 (MQ=255)
                                                                                                                           cAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGCCGACCGCTAGAATCATCACGACGCGGAGCGCGGCCTTCacgc                           >  1:1036632/1‑149 (MQ=255)
                                                                                                                           cAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCacga                           >  2:870587/1‑150 (MQ=255)
                                                                                                                           cAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCacga                           >  2:1116251/1‑150 (MQ=255)
                                                                                                                             aaaGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCacgacg                         >  1:688303/1‑150 (MQ=255)
                                                                                                                             aaaGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTACacgacg                         >  1:705286/1‑150 (MQ=255)
                                                                                                                              aaGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGCCGt                        >  1:998727/1‑150 (MQ=255)
                                                                                                                                  cAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgc                    >  2:768445/1‑150 (MQ=255)
                                                                                                                                  cAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTAACCACGTTcgc                    >  1:800772/1‑150 (MQ=255)
                                                                                                                                  cAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTCTATAAATTATATTACAGATTGTGTTCGCTGATCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGAAGCATCATCACGACGAGGAGCGCGGCCTTCACGACGTTcgc                    >  1:2230/1‑150 (MQ=255)
                                                                                                                                   aGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgcc                   <  1:1737877/150‑1 (MQ=255)
                                                                                                                                    gAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACTGTTTGTGTTCGCTGTTAAGCGATGATTACGCATCACCCCCGCAACGACGACGACCGGTCGCATCATCACGACGCGGAGCGCGGCCTTCACGACGTTctccg                  >  2:260368/1‑150 (MQ=255)
                                                                                                                                    gAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGCCGCGGAGCGCGGCCTTCACGACGTTcgccg                  >  2:136802/1‑150 (MQ=255)
                                                                                                                                    gAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgccg                  >  1:1072547/1‑150 (MQ=255)
                                                                                                                                       aaaaaaGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCCCCACCGACCCGACGACGCCCGGTAGAATCTTCACGCCGCGGCGCGCGGCGTTCCCGCCGTTCGCCGCt                >  1:1009214/1‑150 (MQ=255)
                                                                                                                                       aaaaaaGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCt                >  2:1825701/1‑150 (MQ=255)
                                                                                                                                            aGCCCCGATTTTTACCATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCTGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCGAAAac           >  1:172474/1‑150 (MQ=255)
                                                                                                                                            aGCCCCGATTTTTACCATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCACTAAAac           >  1:765913/1‑150 (MQ=255)
                                                                                                                                             gCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAacg          <  1:552701/150‑1 (MQ=255)
                                                                                                                                             gCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAacg          >  2:162363/1‑150 (MQ=255)
                                                                                                                                             gCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTACAATAAACACCACGCGGAGCACGGCATTCACGACCTTCGCACCAAAAacc          >  2:1208414/1‑149 (MQ=255)
                                                                                                                                                 cGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAAcgacga      <  1:1239031/150‑1 (MQ=255)
                                                                                                                                                     ggTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCAt  >  2:416779/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
ACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCAT  >  NC_000913/3305765‑3306062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: