Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,305,916 A→T 6.5% intergenic (‑99/+55) mtr ← / ← deaD tryptophan transporter of high affinity/ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,305,9160AT 6.5% 303.1 / 8.0 102intergenic (‑99/+55)mtr/deaDtryptophan transporter of high affinity/ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (50/45);  new base T (2/5);  total (52/50)
Fisher's exact test for biased strand distribution p-value = 2.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 103 total aligned reads displayed.

GCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCA  >  NC_000913/3305770‑3306064
                                                                                                                                                   |                                                                                                                                                    
gccgccAAGCAGCGACGGTGACGTTGGGGTGGTGGTTAGTGTTGCCCTGAGGGCTGCTCTCCAGTGAAAAATAGTGCGCCTGCGTTGTTATGCATTGCACTGTACCAGTACACTAGTACAACCGACAGAAAAAAAGCCCCGATGGaaaca                                                                                                                                                   >  1:319652/1‑148 (MQ=255)
     cAAGCAGCGACGGTGACGTTTGGGTGGTGGTATGGGTTGCCATGTGGTCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACATAAGACAGAAAATAAGCCCCGATGGTTAAAATCgg                                                                                                                                              <  2:633390/150‑1 (MQ=255)
        gcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGc                                                                                                                                           <  2:624126/150‑1 (MQ=255)
        gcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGc                                                                                                                                           <  2:1256566/150‑1 (MQ=255)
        gcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGc                                                                                                                                           >  1:1488611/1‑150 (MQ=255)
         cagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGCt                                                                                                                                          <  2:620965/150‑1 (MQ=255)
         cagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCAGATGGTAAAAATCGGGGCt                                                                                                                                          >  2:1239160/1‑150 (MQ=255)
           gcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGCttt                                                                                                                                        <  2:1497796/150‑3 (MQ=255)
            cGACGGTGACGTTTGGGTGGTGGTTAGTGTGGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGta                                                                                                                                       <  1:1847609/150‑1 (MQ=255)
            cGACGGTGACGTTTGGGTGGTGGTTAGTGGTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAACATCGGGGCTGta                                                                                                                                       <  2:1592683/150‑1 (MQ=255)
             gccGGTGACGTTTGGGTGGTGGGTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGGGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAACACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtat                                                                                                                                      <  1:830048/148‑1 (MQ=255)
              aCGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtata                                                                                                                                     <  1:1025021/150‑1 (MQ=255)
               cGGTGACGTTTGGGTGGTTGTTAGTGTTGCCATGAGGTCTTATCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGAACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtatat                                                                                                                                    <  1:123867/150‑1 (MQ=255)
               cGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCTCTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtatat                                                                                                                                    >  2:552701/1‑150 (MQ=255)
                     cggttGGGGCGTGGTTAGTGTGGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGTGTTGTATATattatt                                                                                                                              <  2:364852/147‑1 (MQ=255)
                      gTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTAttt                                                                                                                             >  2:1204390/1‑150 (MQ=255)
                         tGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTAc                                                                                                                          <  1:1035203/150‑1 (MQ=255)
                          gggTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACa                                                                                                                         <  1:1624256/150‑1 (MQ=255)
                           ggtggtggtTAGTGTTGCCATGAGGGCTTCTCACCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACTAGTACAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGa                                                                                                                       <  2:1194739/150‑1 (MQ=255)
                            gtggtggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAATACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGa                                                                                                                       <  1:1271774/150‑1 (MQ=255)
                            gagggggtTAGTGTTGCCATGAGGGCGTCTCTCCAGTGAAAAATAGTCCGACTGCGCTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCAGATGTTAAAAATCGGGGCTGTATATATTATTTTACAGa                                                                                                                       <  2:1655283/145‑1 (MQ=255)
                               gtggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtg                                                                                                                    <  1:401137/150‑1 (MQ=255)
                               gtggtAAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtg                                                                                                                    <  2:336385/150‑1 (MQ=255)
                                  gtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTgtgt                                                                                                                 >  2:1430306/1‑150 (MQ=255)
                                     aGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCg                                                                                                              <  1:419377/150‑1 (MQ=255)
                                      gtgtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGc                                                                                                             <  2:1376228/150‑1 (MQ=255)
                                      gtgtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGAGACTGCGTTGTTATACATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGc                                                                                                             <  1:70990/150‑1 (MQ=255)
                                        gtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGGTGTTATGCATTGCACTGTACCTGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTg                                                                                                           <  2:1513589/150‑1 (MQ=255)
                                         ttGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGt                                                                                                          <  2:1754603/150‑1 (MQ=255)
                                          tGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGtt                                                                                                         <  2:381631/150‑1 (MQ=255)
                                           gCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGtt                                                                                                         >  1:1328195/1‑150 (MQ=255)
                                            ccATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCa                                                                                                       <  1:576333/150‑1 (MQ=255)
                                              aTGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGc                                                                                                     <  1:214244/150‑1 (MQ=255)
                                                 tgggCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgat                                                                                                  <  2:207653/149‑1 (MQ=255)
                                                  gggCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCTATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgatg                                                                                                 <  2:41432/150‑1 (MQ=255)
                                                   ggCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgatga                                                                                                >  1:889268/1‑150 (MQ=255)
                                                          ctcCAGTTAAAAATAGTGCGACTGCGTTGTTATGCATTTCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCa                                                                                         <  1:372923/150‑1 (MQ=255)
                                                          ctcCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCa                                                                                         <  1:632361/150‑1 (MQ=255)
                                                            ccAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATc                                                                                       >  1:1391197/1‑150 (MQ=255)
                                                               gTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcacc                                                                                    >  1:1576505/1‑150 (MQ=255)
                                                               gTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcacc                                                                                    >  2:805299/1‑150 (MQ=255)
                                                               gTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcacc                                                                                    >  2:1239031/1‑150 (MQ=255)
                                                                 gAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcaccac                                                                                  >  2:12142/1‑150 (MQ=255)
                                                                  aaaaaTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCaccacc                                                                                 <  1:879578/150‑1 (MQ=255)
                                                                   aaaaTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCg                                                                                >  2:1737877/1‑150 (MQ=255)
                                                                        aGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacg                                                                           >  2:439762/1‑150 (MQ=255)
                                                                        aGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacg                                                                           >  2:1700427/1‑150 (MQ=255)
                                                                        aGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacg                                                                           <  2:836947/150‑1 (MQ=255)
                                                                         gTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacga                                                                          >  2:331556/1‑150 (MQ=255)
                                                                           ccGACTGCGTTGTTATGCATTGCACTGGACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacg                                                                        <  2:1369090/149‑1 (MQ=255)
                                                                             taCTGCGTTGTAATGCATTGCACTGTACCAGTACACTAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacgac                                                                      <  2:436474/149‑1 (MQ=255)
                                                                             gACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacgac                                                                      >  2:1123007/1‑150 (MQ=255)
                                                                             gACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGCGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacgac                                                                      >  1:178431/1‑150 (MQ=255)
                                                                              aCTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAGGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacgacg                                                                     <  1:827373/150‑1 (MQ=255)
                                                                                   gttgttATGCATTGCACTGTACACGAACACGAGTACAAAAGACAGAAAAAACGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTGCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCgg                                                                <  2:1690519/150‑1 (MQ=255)
                                                                                    ttgttATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGt                                                               <  2:1851535/150‑1 (MQ=255)
                                                                                     tgttATGCATTTCACTGTACCAGAACACGAGTACAAAAGACATAAAAAAAGCCCCGATGTTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTa                                                              <  2:1666930/150‑1 (MQ=255)
                                                                                      gttATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAg                                                             >  1:948395/1‑150 (MQ=255)
                                                                                      gttATGCATTGCACTGTACCAGTACACGAGTACAAAAGACACAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAg                                                             <  2:1345943/150‑1 (MQ=255)
                                                                                        tATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTAATTTAAAGATTGTTTTCTCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGAAGCCCGGTAGaa                                                           >  2:292488/1‑150 (MQ=255)
                                                                                         aTGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAat                                                          <  1:1232216/150‑1 (MQ=255)
                                                                                           gCATTGCACTGTACCTCTACACGAGAACAACAGACACAAAAACAGCCCCGACCGGAAAAATCGGGGCTGTATATATTATTTAACAGATTGTCTTCGCTGTTCAGCGATGATTACGCATCACCCCCGAAACGACGCCGACCGGTAGAatca                                                        <  2:699897/150‑1 (MQ=255)
                                                                                           gCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGGAGAatca                                                        >  2:304228/1‑150 (MQ=255)
                                                                                             aTTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatcctc                                                      >  2:262313/1‑150 (MQ=255)
                                                                                             aTTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatcatc                                                      >  1:798407/1‑150 (MQ=255)
                                                                                                   cTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCacgacg                                                >  2:601955/1‑150 (MQ=255)
                                                                                                     gTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGAGGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGAcgcg                                              >  2:1753975/1‑150 (MQ=255)
                                                                                                      tACCAGTACATGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCAACACCGATACGACGACGACCGGTAGAATCATCACGACGCgg                                             >  1:400567/1‑150 (MQ=255)
                                                                                                      tACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCgg                                             >  1:42467/1‑150 (MQ=255)
                                                                                                        ccAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAg                                           >  1:1065353/1‑150 (MQ=255)
                                                                                                             acacCAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCgg                                      <  2:1782196/150‑1 (MQ=255)
                                                                                                              cacGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGc                                     <  1:1263117/150‑1 (MQ=255)
                                                                                                              cacGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGAAGAATCATCACGACCCGGAGCGCGGc                                     >  2:1162897/1‑150 (MQ=255)
                                                                                                                cGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGCCGACCGGTAGAATCATCACGACGCGGAGCGCGGCCt                                   >  1:375256/1‑150 (MQ=255)
                                                                                                                    tACAAAAGCCAGAAAAAAAGCCCCTATGGTAATATTCGGGGCTGTATATATTATTTTACATATTGTGTTGTATGTTCAGCGTTGATTACGAACCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCac                               <  1:1430306/150‑1 (MQ=255)
                                                                                                                     aCAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGCCCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCacg                              >  2:449437/1‑150 (MQ=255)
                                                                                                                     aCAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCacg                              >  1:439544/1‑150 (MQ=255)
                                                                                                                      cAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGCCGACCGCTAGAATCATCACGACGCGGAGCGCGGCCTTCacgc                             >  1:1036632/1‑149 (MQ=255)
                                                                                                                      cAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCacga                             >  2:870587/1‑150 (MQ=255)
                                                                                                                      cAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCacga                             >  2:1116251/1‑150 (MQ=255)
                                                                                                                        aaaGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTACacgacg                           >  1:705286/1‑150 (MQ=255)
                                                                                                                         aaGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGCCGt                          >  1:998727/1‑150 (MQ=255)
                                                                                                                             cAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgc                      >  2:768445/1‑150 (MQ=255)
                                                                                                                             cAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTAACCACGTTcgc                      >  1:800772/1‑150 (MQ=255)
                                                                                                                             cAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTCTATAAATTATATTACAGATTGTGTTCGCTGATCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGAAGCATCATCACGACGAGGAGCGCGGCCTTCACGACGTTcgc                      >  1:2230/1‑150 (MQ=255)
                                                                                                                              aGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgcc                     <  1:1737877/150‑1 (MQ=255)
                                                                                                                               gAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACTGTTTGTGTTCGCTGTTAAGCGATGATTACGCATCACCCCCGCAACGACGACGACCGGTCGCATCATCACGACGCGGAGCGCGGCCTTCACGACGTTctccg                    >  2:260368/1‑150 (MQ=255)
                                                                                                                               gAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGCCGCGGAGCGCGGCCTTCACGACGTTcgccg                    >  2:136802/1‑150 (MQ=255)
                                                                                                                               gAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgccg                    >  1:1072547/1‑150 (MQ=255)
                                                                                                                                  aaaaaaGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCCCCACCGACCCGACGACGCCCGGTAGAATCTTCACGCCGCGGCGCGCGGCGTTCCCGCCGTTCGCCGCt                  >  1:1009214/1‑150 (MQ=255)
                                                                                                                                  aaaaaaGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCt                  >  2:1825701/1‑150 (MQ=255)
                                                                                                                                       aGCCCCGATTTTTACCATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCTGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCGAAAac             >  1:172474/1‑150 (MQ=255)
                                                                                                                                       aGCCCCGATTTTTACCATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCACTAAAac             >  1:765913/1‑150 (MQ=255)
                                                                                                                                        gCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAacg            >  2:162363/1‑150 (MQ=255)
                                                                                                                                        gCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAacg            <  1:552701/150‑1 (MQ=255)
                                                                                                                                        gCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTACAATAAACACCACGCGGAGCACGGCATTCACGACCTTCGCACCAAAAacc            >  2:1208414/1‑149 (MQ=255)
                                                                                                                                            cGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAAcgacga        <  1:1239031/150‑1 (MQ=255)
                                                                                                                                                ggTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCAt    >  2:416779/1‑150 (MQ=255)
                                                                                                                                                  tAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATca  <  2:889268/150‑1 (MQ=255)
                                                                                                                                                  tAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGATCGCCGCTAAAACGACGACCATcc  >  1:751333/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCA  >  NC_000913/3305770‑3306064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: