Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,360,643 T→A 6.7% intergenic (+27/‑533) gltD → / → gltF glutamate synthase, 4Fe‑4S protein, small subunit/periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,360,6430TA 6.7% 346.4 / 8.9 123intergenic (+27/‑533)gltD/gltFglutamate synthase, 4Fe‑4S protein, small subunit/periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (60/53);  new base A (7/2);  total (68/55)
Fisher's exact test for biased strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

CAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAGCAATTTC  >  NC_000913/3360494‑3360792
                                                                                                                                                     |                                                                                                                                                     
 aGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGATCCGAACAGCTGGAGACCGCTATTGCCGAAGGACGAAAGGCGGCAGCCGGTATTATGAACAGGCTGCAAGTTTAAGCGAGGAAACAATGAATACGCCAGACtg                                                                                                                                                      >  1:70145/1‑150 (MQ=255)
  gACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACtgt                                                                                                                                                     >  1:602100/1‑150 (MQ=255)
  gACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACtgt                                                                                                                                                     >  2:672483/1‑150 (MQ=255)
       gCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTg                                                                                                                                                <  2:655466/150‑1 (MQ=255)
         aaCCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGCCCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTTGc                                                                                                                                              >  2:350342/1‑150 (MQ=255)
         aaCCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGc                                                                                                                                              <  2:1742906/150‑1 (MQ=255)
         aaCCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTACGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGc                                                                                                                                              >  2:1621313/1‑150 (MQ=255)
          ccccGAACATCTTAGCGGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATAATCAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCg                                                                                                                                             <  2:182631/149‑1 (MQ=255)
          aCCCGAACATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCg                                                                                                                                             <  2:1593604/150‑1 (MQ=255)
          aCCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACACAGTCTGGCg                                                                                                                                             <  1:1289112/150‑1 (MQ=255)
          aCCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACACAGTCTGGCg                                                                                                                                             <  1:1368194/150‑1 (MQ=255)
             cGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGCACGGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCCGACAGTGTCTGGCGaaa                                                                                                                                          >  2:935736/1‑149 (MQ=255)
              gAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATc                                                                                                                                         <  1:1605333/150‑1 (MQ=255)
                aaaTCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCTCCAGACTGTGTCTGGCGAATCaa                                                                                                                                       >  1:886022/1‑150 (MQ=255)
                    cTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCTCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTa                                                                                                                                   >  1:436131/1‑150 (MQ=255)
                         cTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGCCCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGAc                                                                                                                              >  1:1206999/1‑150 (MQ=255)
                           ggcggcGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGa                                                                                                                            >  2:1247891/1‑150 (MQ=255)
                               gcgATATCGTCCGTGGGTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTg                                                                                                                        <  2:142518/150‑1 (MQ=255)
                                    atCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCgt                                                                                                                   >  1:93423/1‑150 (MQ=255)
                                     tCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCgtt                                                                                                                  >  1:310550/1‑150 (MQ=255)
                                      cGTCCGTGGTGCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCgttg                                                                                                                 <  1:691503/150‑1 (MQ=255)
                                       gTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTATGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCgttgt                                                                                                                <  1:434797/150‑1 (MQ=255)
                                           gTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGtttta                                                                                                            >  2:377413/1‑149 (MQ=255)
                                            tGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCt                                                                                                           <  1:845354/150‑1 (MQ=255)
                                              gTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTgg                                                                                                         >  1:1458344/1‑150 (MQ=255)
                                              gTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTgg                                                                                                         <  1:914208/150‑1 (MQ=255)
                                              gTTCCGATCTGGTGGTCACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTCGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTgg                                                                                                         <  2:970834/150‑1 (MQ=255)
                                                tCCGATCTGGGGGTGACCGCTATTGCCGAAGGGCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGCCGAAGTGCGCGTTGTTTTCTGGCa                                                                                                       <  2:1777025/150‑1 (MQ=255)
                                                  cGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCaaa                                                                                                     >  1:856601/1‑150 (MQ=255)
                                                  cGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCaaa                                                                                                     >  1:1145943/1‑150 (MQ=255)
                                                        ggtggtGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGc                                                                                               >  2:110885/1‑150 (MQ=255)
                                                           ggtgACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATg                                                                                            >  1:1777969/1‑150 (MQ=255)
                                                            gtgACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCTAGTTGTTTTATGGAAAATGCGGCATGa                                                                                           >  1:1107776/1‑150 (MQ=255)
                                                             tgACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTTTCGGGCGAATCAAAATAAAGACGAAGTGCGCGTTGTTTTATTGAAAATGCGGGAagag                                                                                          >  2:1696217/1‑150 (MQ=255)
                                                              gACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGTAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACGGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATgcgc                                                                                         >  1:155562/1‑150 (MQ=255)
                                                              gACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTTTGTCAGGCGAATCAAATTAATGACGAAGTTCGCGTTGTTATATGGCAAAATCGGCATGAGc                                                                                         >  2:1674945/1‑150 (MQ=255)
                                                              gACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGc                                                                                         >  1:882054/1‑150 (MQ=255)
                                                                ccGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGt                                                                                       >  1:318694/1‑150 (MQ=255)
                                                                ccGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGt                                                                                       >  1:847990/1‑150 (MQ=255)
                                                                ccGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGt                                                                                       >  2:738315/1‑150 (MQ=255)
                                                                  gCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGttt                                                                                     <  2:1291877/150‑1 (MQ=255)
                                                                   cTATTGCCTAAGGTCGTAAGGCGGCAGACGGTATTATGTACTGGCGGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGGCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGTGCGTTTg                                                                                    <  1:341832/150‑1 (MQ=255)
                                                                     aTTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGAc                                                                                  >  1:245398/1‑150 (MQ=255)
                                                                       tGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCt                                                                                >  2:1402299/1‑150 (MQ=255)
                                                                        gCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGCCCtt                                                                               >  1:1757610/1‑150 (MQ=255)
                                                                        gCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCtt                                                                               >  1:1004111/1‑150 (MQ=255)
                                                                         ccGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCttt                                                                              >  1:357547/1‑150 (MQ=255)
                                                                          cGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTc                                                                             <  2:1555224/150‑1 (MQ=255)
                                                                              ggTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCtat                                                                         >  1:978392/1‑150 (MQ=255)
                                                                                 ggTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTataat                                                                      <  1:368496/149‑1 (MQ=255)
                                                                                  gTAAGGAGGCAGACGGTATTATGAACTGTCTGGATGTTTAAGCGAGGTAACAATGAATTCGCCTTTCTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTataata                                                                     <  2:70145/150‑1 (MQ=255)
                                                                                    aaGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAgg                                                                   >  1:1802534/1‑150 (MQ=255)
                                                                                    aaGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAgg                                                                   <  2:1577559/150‑1 (MQ=255)
                                                                                    aaGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAgg                                                                   <  1:527440/150‑1 (MQ=255)
                                                                                     aGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAggg                                                                  <  2:1362413/150‑1 (MQ=255)
                                                                                     aGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAggg                                                                  <  2:637271/150‑1 (MQ=255)
                                                                                      ggcggcAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGa                                                                 <  1:180647/150‑1 (MQ=255)
                                                                                      ggcggcAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGa                                                                 >  2:1442660/1‑150 (MQ=255)
                                                                                           cAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTTAAGACGAACTGCGCGTTGTTTTATGGCAAATGCGGCATGAGCGTTTGACCTTTACTATAATAGGGATAAtt                                                            >  1:874360/1‑150 (MQ=255)
                                                                                             gACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAAtttt                                                          >  1:1589794/1‑150 (MQ=255)
                                                                                             gACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGATGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAAtttt                                                          >  1:1005484/1‑150 (MQ=255)
                                                                                              aCGTTATTATGAACTGTCTTGAAGTTTAAGAGAGTTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTc                                                         <  1:1313093/150‑1 (MQ=255)
                                                                                              aCGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTc                                                         <  1:738315/150‑1 (MQ=255)
                                                                                               cGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCa                                                        <  2:1792016/150‑1 (MQ=255)
                                                                                                ggTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCaa                                                       <  1:377413/150‑1 (MQ=255)
                                                                                                      atGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAAtt                                                 >  2:256688/1‑150 (MQ=255)
                                                                                                       tGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATtg                                                <  1:534006/150‑1 (MQ=255)
                                                                                                        tatctggatggAAGTTTAAGCGAGGTCACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGCCGAAGTGCGCGGTGGTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATtgt                                               <  1:891096/147‑1 (MQ=255)
                                                                                                        gAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATtgt                                               >  2:659058/1‑150 (MQ=255)
                                                                                                         aaCTGTCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGGGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTATCAATAAATtgtg                                              <  2:76053/150‑1 (MQ=255)
                                                                                                          aCTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGc                                             <  1:1410862/150‑1 (MQ=255)
                                                                                                           ctggctggAAGTTTAAGCGAGTTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCt                                            <  2:1262560/150‑1 (MQ=255)
                                                                                                             ggctggAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCttt                                          >  1:1156195/1‑150 (MQ=255)
                                                                                                              gctggAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTg                                         <  1:449914/150‑1 (MQ=255)
                                                                                                               ctggAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGt                                        >  2:1551687/1‑150 (MQ=255)
                                                                                                               ctggAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGt                                        >  1:203283/1‑150 (MQ=255)
                                                                                                               ctggAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGAAGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGt                                        >  2:1381165/1‑150 (MQ=255)
                                                                                                                  gAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCa                                     <  1:1040825/150‑1 (MQ=255)
                                                                                                                   aaGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTCGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCAc                                    <  2:758775/150‑1 (MQ=255)
                                                                                                                      tttAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGACTCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCAcctc                                 >  1:312561/1‑148 (MQ=255)
                                                                                                                      tttAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTACTATAATAGGGATAATTTTCAATAAATTTTGCTTTGTTCACCaa                                 >  1:1393403/1‑150 (MQ=255)
                                                                                                                         aaGCGAGGTAACAAAGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTc                              <  2:1659759/150‑1 (MQ=255)
                                                                                                                           gggAGGTCACAATGATTTTGCCAGAATGTGTCTGGCGAATCAAATTACTGACGACGTGCGCGTTGTTTTCTGGCTAATGCGTCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCtg                            <  1:935066/148‑1 (MQ=255)
                                                                                                                              aGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCtgatg                         <  1:68644/150‑1 (MQ=255)
                                                                                                                              aGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCtgatg                         >  1:376577/1‑150 (MQ=255)
                                                                                                                                  aaCAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTg                     >  2:634470/1‑150 (MQ=255)
                                                                                                                                  aaCAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTg                     >  1:503678/1‑150 (MQ=255)
                                                                                                                                  aaCAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTg                     <  2:96670/150‑1 (MQ=255)
                                                                                                                                     aaTGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAg                  >  1:1001280/1‑150 (MQ=255)
                                                                                                                                        tgaTTCGCCAGACACAGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCATTAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGctc               >  1:217146/3‑148 (MQ=255)
                                                                                                                                        tgaTTCGCCAGACACAGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGcac               >  2:1761978/3‑150 (MQ=255)
                                                                                                                                        gAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGcac               <  1:653512/150‑1 (MQ=255)
                                                                                                                                          aTTCGCCAGACACAGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCCCAt             >  2:1226195/1‑150 (MQ=255)
                                                                                                                                           ttCGCCAGACACAGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATc            >  2:131429/1‑150 (MQ=255)
                                                                                                                                           ttCGCCAGACACAGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATc            >  1:622037/1‑150 (MQ=255)
                                                                                                                                           ttCGCCAGACACAGTCTGGCGAATCAAATTACTGACGAAGTGAGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATc            >  2:1062991/1‑150 (MQ=255)
                                                                                                                                             cGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCaa          <  1:566094/150‑1 (MQ=255)
                                                                                                                                              gCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAg         <  2:36192/150‑1 (MQ=255)
                                                                                                                                                   aCTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAGCAAtt    >  2:672035/1‑150 (MQ=255)
                                                                                                                                                     tgtgTATGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAGCAATTtc  <  1:1325936/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAGCAATTTC  >  NC_000913/3360494‑3360792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: