Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 394,472 G→A 8.3% intergenic (+54/‑34) yaiT → / → yaiV pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein/putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913394,4720GA 8.3% 322.3 / 12.2 108intergenic (+54/‑34)yaiT/yaiVpseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein/putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (59/39);  new base A (5/4);  total (64/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

AAGAACTTCAGCGCCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATGT  >  NC_000913/394323‑394621
                                                                                                                                                     |                                                                                                                                                     
aaGAACTTCAGCGCCTATACCGATGCTAACTACTTCGGTTGTTGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAg                                                                                                                                                       <  1:1016450/150‑1 (MQ=255)
aaGAACTTCAGCGCCTATACCGATGCTAACTACCTCGGTGGTGGTGCCGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAg                                                                                                                                                       >  1:582407/1‑150 (MQ=255)
aaGAACTTCAGCGCCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAg                                                                                                                                                       >  1:1541234/1‑150 (MQ=255)
aaGAACTTCAGCGCATATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAg                                                                                                                                                       <  2:1617469/150‑1 (MQ=255)
     cTTCAGCGCCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGAttt                                                                                                                                                  >  1:1117172/1‑150 (MQ=255)
     cTTCAGCGCCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGAttt                                                                                                                                                  >  1:1132525/1‑150 (MQ=255)
     cTTCAGCGCCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGAttt                                                                                                                                                  <  2:1828988/150‑1 (MQ=255)
         aGCGCCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACg                                                                                                                                              <  2:1099940/150‑1 (MQ=255)
          gcgcCTATACCGATGCTAACTACCTCTTTGGTGGTGACGTAGATCAAGACTGGTCCGCGTACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTACGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTTACgg                                                                                                                                             <  1:560660/150‑1 (MQ=255)
           cgcCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACggg                                                                                                                                            <  1:671484/150‑1 (MQ=255)
           cgcCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAATCCTTGACggg                                                                                                                                            <  1:1578966/150‑1 (MQ=255)
            gcCTATACCGATGCTATCTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGc                                                                                                                                           <  2:684831/150‑1 (MQ=255)
            gcCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTTTTAAATATTCATGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGc                                                                                                                                           <  2:1223566/150‑1 (MQ=255)
            gcCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAATCCTTGACGGGc                                                                                                                                           <  2:405779/150‑1 (MQ=255)
            gcCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAATCCTTGACGGGc                                                                                                                                           <  1:1410396/150‑1 (MQ=255)
             ccTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCa                                                                                                                                          <  2:730520/150‑1 (MQ=255)
             ccTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCa                                                                                                                                          <  1:52130/150‑1 (MQ=255)
               tataCCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCAtt                                                                                                                                        >  2:493702/1‑150 (MQ=255)
                  aCCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACt                                                                                                                                     <  1:1246701/150‑1 (MQ=255)
                  aCCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACt                                                                                                                                     >  1:1449603/1‑150 (MQ=255)
                   ccGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTg                                                                                                                                    >  1:1358184/1‑150 (MQ=255)
                     gATGCTATCTCCCTCGGTGGGTGGGACGTAGATCAAGACTGGTCCGCGAACGGGGGTGTTAAATATACCTGGTAATATTCTTCACTCAGAATAAATACTGTTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCa                                                                                                                                  <  2:1128774/150‑1 (MQ=255)
                     gATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCa                                                                                                                                  >  2:1178864/1‑150 (MQ=255)
                     gATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAATCCTTGACGGGCATTatttag                                                                                                                                  <  1:1530096/150‑6 (MQ=255)
                      aTGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCaa                                                                                                                                 >  1:213074/1‑150 (MQ=255)
                         cTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAATGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGAAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAgg                                                                                                                              >  1:1270471/1‑150 (MQ=255)
                         cTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAgg                                                                                                                              >  1:693183/1‑150 (MQ=255)
                          tAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGa                                                                                                                             >  2:535443/1‑150 (MQ=255)
                           aaCTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGAc                                                                                                                            >  2:674927/1‑150 (MQ=255)
                            aCTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGAcg                                                                                                                           >  1:1677690/1‑150 (MQ=255)
                             cTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGAcgc                                                                                                                          <  1:1777568/150‑1 (MQ=255)
                               aCCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACgcgc                                                                                                                        >  1:476155/1‑150 (MQ=255)
                                        gtggttACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTAt                                                                                                               <  2:1388952/150‑1 (MQ=255)
                                            tgACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATTCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGt                                                                                                           >  2:1654380/1‑150 (MQ=255)
                                             gACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAg                                                                                                         >  1:1510484/1‑150 (MQ=255)
                                               cGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGt                                                                                                        >  2:946800/1‑150 (MQ=255)
                                               cGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGt                                                                                                        <  2:1193890/150‑1 (MQ=255)
                                                 tAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCATAAGTTATCTGTAGTTa                                                                                                      >  2:1688551/1‑150 (MQ=255)
                                                 tAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTa                                                                                                      >  1:1861191/1‑150 (MQ=255)
                                                   taTCAAGACTGGGCAGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTaaa                                                                                                    <  1:1635069/149‑1 (MQ=255)
                                                    aTCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAAc                                                                                                   <  2:1862023/150‑1 (MQ=255)
                                                     tCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAAcc                                                                                                  >  1:1743253/1‑150 (MQ=255)
                                                      cAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACct                                                                                                 >  2:572564/1‑150 (MQ=255)
                                                       aaGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACctc                                                                                                >  1:447267/1‑150 (MQ=255)
                                                        aGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACctct                                                                                               <  1:459147/150‑1 (MQ=255)
                                                            tGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAg                                                                                           >  1:1234237/1‑150 (MQ=255)
                                                             ggTCCGCGAACGTGGGTGGTATATATCCCTTGTAATATTCTTCACTCCGAAGAAATACTGTTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAgg                                                                                          <  2:172817/150‑1 (MQ=255)
                                                                ccGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCAATCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCAGTCAAGGATTTGACGGGCATTACTGCAAAGGCCGCGCAAATGTTATCTGTAGTTAAACCTATTCAGGAAt                                                                                       >  2:879920/1‑150 (MQ=255)
                                                                 cgcgAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAAtt                                                                                      >  2:794505/1‑150 (MQ=255)
                                                                    gAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTgg                                                                                   <  2:63293/150‑1 (MQ=255)
                                                                         tGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGc                                                                              <  1:420841/150‑1 (MQ=255)
                                                                          gggTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCt                                                                             >  1:879767/1‑150 (MQ=255)
                                                                           ggTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTc                                                                            <  2:1042291/150‑1 (MQ=255)
                                                                           ggTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTc                                                                            <  1:555805/150‑1 (MQ=255)
                                                                            gtgtTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCg                                                                           >  1:1249155/1‑150 (MQ=255)
                                                                            gtgtTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCg                                                                           <  2:800961/150‑1 (MQ=255)
                                                                               ttAAATATACCTGGTAATATTATTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGGCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATa                                                                        <  1:1375463/150‑1 (MQ=255)
                                                                                    tataCCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGt                                                                   >  1:619900/1‑150 (MQ=255)
                                                                                      taCCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGttt                                                                 >  1:1598113/1‑150 (MQ=255)
                                                                                       aCCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATCTGGTAAGCTCGATAAATGTTTg                                                                <  1:228194/150‑1 (MQ=255)
                                                                                         cTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTc                                                              >  1:1365034/1‑150 (MQ=255)
                                                                                         cTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTc                                                              >  2:1470980/1‑150 (MQ=255)
                                                                                           ggTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTcaa                                                            >  1:7541/1‑148 (MQ=255)
                                                                                            gTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAg                                                           >  2:462097/1‑150 (MQ=255)
                                                                                                 atTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACg                                                      >  1:1729009/1‑150 (MQ=255)
                                                                                                  ttcttcACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACgg                                                     >  1:1289344/1‑150 (MQ=255)
                                                                                                    catcACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTa                                                   <  2:306480/148‑1 (MQ=255)
                                                                                                     ttcACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTAc                                                  >  2:1537101/1‑150 (MQ=255)
                                                                                                      tcACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTAcg                                                 >  2:1256533/1‑150 (MQ=255)
                                                                                                            cGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCtta                                           >  1:319754/1‑149 (MQ=255)
                                                                                                            cGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTc                                           <  1:1251101/150‑1 (MQ=255)
                                                                                                             gaagaaATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCTAATGTTATGTGTAGTTAATCCTCTTCAGGAATTTGGTAAGCTCGATATATGTTTGTCGAGATACGGTAcgagggtcg                                          >  2:232476/1‑143 (MQ=255)
                                                                                                              aagaaATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGa                                         >  2:1861064/1‑150 (MQ=255)
                                                                                                                   aTACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTta                                    <  1:1704978/150‑1 (MQ=255)
                                                                                                                    tACTGGTAATTTAATCTAAATAATGCCCGTCATGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTtaa                                   >  1:1465988/1‑150 (MQ=255)
                                                                                                                    tACTGGTAATTTAATCTAAATAATGCCAGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTtaa                                   <  1:106653/150‑1 (MQ=255)
                                                                                                                         gTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATg                              >  2:322929/1‑150 (MQ=255)
                                                                                                                           aaTTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGaa                            <  1:950892/150‑1 (MQ=255)
                                                                                                                             tttAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGaaaa                          <  1:1460095/150‑1 (MQ=255)
                                                                                                                              ttAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAg                         <  1:1864752/150‑1 (MQ=255)
                                                                                                                              ttAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAg                         <  1:1871916/150‑1 (MQ=255)
                                                                                                                                aaTCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCa                       >  2:644112/1‑150 (MQ=255)
                                                                                                                                   cTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGt                    >  2:1136429/1‑150 (MQ=255)
                                                                                                                                    tAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGtt                   >  1:1793874/1‑150 (MQ=255)
                                                                                                                                    tAAATAATGCCAGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGtt                   <  2:1541234/150‑1 (MQ=255)
                                                                                                                                      aataatGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTtat                 >  2:306778/1‑150 (MQ=255)
                                                                                                                                      aataatGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTtat                 >  2:1727770/1‑150 (MQ=255)
                                                                                                                                         aatGCCCGTCAAATCCTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATAtt              >  1:1312051/1‑150 (MQ=255)
                                                                                                                                          atGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATAttt             >  2:1320740/1‑150 (MQ=255)
                                                                                                                                            gCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTc           >  1:1014327/1‑150 (MQ=255)
                                                                                                                                            gCCCGTCAAATCCTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTc           >  1:79710/1‑150 (MQ=255)
                                                                                                                                            gCCCGTCAAATCCTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAACCAAGTTATATTTTc           >  2:784578/1‑150 (MQ=255)
                                                                                                                                             cccGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTcc          <  2:1510484/150‑1 (MQ=255)
                                                                                                                                             cccGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTcc          >  2:558702/1‑150 (MQ=255)
                                                                                                                                               cGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAg        >  1:844324/1‑150 (MQ=255)
                                                                                                                                                 tCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTg      <  1:794505/150‑1 (MQ=255)
                                                                                                                                                    tggATTTGACTGGCATTACTGCAAATGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGATTTTTGTAAGATCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATg   <  1:1421661/149‑1 (MQ=255)
                                                                                                                                                    aGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATg   <  1:946800/150‑1 (MQ=255)
                                                                                                                                                     ggATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTCCGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATGt  >  1:1146821/1‑150 (MQ=255)
                                                                                                                                                     ggATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAAAGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAATAGCAAGTTATATTTTCAAGTGATGt  >  1:1152793/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAGAACTTCAGCGCCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATGT  >  NC_000913/394323‑394621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: