Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,360,645 T→A 6.5% intergenic (+29/‑531) gltD → / → gltF glutamate synthase, 4Fe‑4S protein, small subunit/periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,360,6450TA 6.5% 349.2 / 11.7 123intergenic (+29/‑531)gltD/gltFglutamate synthase, 4Fe‑4S protein, small subunit/periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (61/52);  new base A (6/2);  total (68/55)
Fisher's exact test for biased strand distribution p-value = 2.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.26e-01

Only 100 of 127 total aligned reads displayed.

GACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAGCAATTTCTC  >  NC_000913/3360496‑3360794
                                                                                                                                                     |                                                                                                                                                     
     gCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTg                                                                                                                                                  <  2:655466/150‑1 (MQ=255)
       aaCCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGCCCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTTGc                                                                                                                                                >  2:350342/1‑150 (MQ=255)
       aaCCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGc                                                                                                                                                <  2:1742906/150‑1 (MQ=255)
        ccccGAACATCTTAGCGGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATAATCAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCg                                                                                                                                               <  2:182631/149‑1 (MQ=255)
        aCCCGAACATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCg                                                                                                                                               <  2:1593604/150‑1 (MQ=255)
        aCCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCg                                                                                                                                               <  2:1657206/150‑1 (MQ=255)
        aCCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACACAGTCTGGCg                                                                                                                                               <  1:1289112/150‑1 (MQ=255)
           cGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGCACGGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCCGACAGTGTCTGGCGaaa                                                                                                                                            >  2:935736/1‑149 (MQ=255)
            gAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATc                                                                                                                                           <  1:589307/150‑1 (MQ=255)
            gAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATc                                                                                                                                           <  1:1605333/150‑1 (MQ=255)
              aaaTCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCaa                                                                                                                                         <  1:1339880/150‑1 (MQ=255)
                 tCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAAtt                                                                                                                                      <  2:376990/150‑1 (MQ=255)
                  cTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCTCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTa                                                                                                                                     >  1:436131/1‑150 (MQ=255)
                      gCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATCGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGa                                                                                                                                 <  1:862462/150‑1 (MQ=255)
                       cTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGCCCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGAc                                                                                                                                >  1:1206999/1‑150 (MQ=255)
                         ggcggcGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGa                                                                                                                              >  2:1247891/1‑150 (MQ=255)
                            ggcgCTATCGTCCGTGGTGCCGATCTGGTGGTGCCCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACGGTGTCTGGCGAATCAAATTACTGCCGAAGt                                                                                                                           >  2:1334239/1‑150 (MQ=255)
                             gcgATATCGTCCGTGGGTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTg                                                                                                                          <  2:142518/150‑1 (MQ=255)
                                  atCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCgt                                                                                                                     >  1:93423/1‑150 (MQ=255)
                                        cGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGtttt                                                                                                               >  1:1655204/1‑150 (MQ=255)
                                         gTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGtttta                                                                                                              >  2:377413/1‑149 (MQ=255)
                                          tGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCt                                                                                                             <  1:845354/150‑1 (MQ=255)
                                            gTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTgg                                                                                                           <  1:914208/150‑1 (MQ=255)
                                            gTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTgg                                                                                                           >  1:1458344/1‑150 (MQ=255)
                                            gTTCCGATCTGGTGGTCACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTCGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTgg                                                                                                           <  2:970834/150‑1 (MQ=255)
                                             ttCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTTGc                                                                                                          >  1:786106/1‑150 (MQ=255)
                                              tCCGATCTGGGGGTGACCGCTATTGCCGAAGGGCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGCCGAAGTGCGCGTTGTTTTCTGGCa                                                                                                         <  2:1777025/150‑1 (MQ=255)
                                                cGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCaaa                                                                                                       >  1:1145943/1‑150 (MQ=255)
                                                    cTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCg                                                                                                   <  1:419027/150‑1 (MQ=255)
                                                      ggtggtGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGc                                                                                                 >  2:110885/1‑150 (MQ=255)
                                                         ggtgACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATg                                                                                              <  2:1558540/150‑1 (MQ=255)
                                                         ggtgACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATg                                                                                              >  1:1777969/1‑150 (MQ=255)
                                                          gtgACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCTAGTTGTTTTATGGAAAATGCGGCATGa                                                                                             >  1:1107776/1‑150 (MQ=255)
                                                            gACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGTAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACGGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATgcgc                                                                                           >  1:155562/1‑150 (MQ=255)
                                                            gACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTTTGTCAGGCGAATCAAATTAATGACGAAGTTCGCGTTGTTATATGGCAAAATCGGCATGAGc                                                                                           >  2:1674945/1‑150 (MQ=255)
                                                            gACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGc                                                                                           >  1:882054/1‑150 (MQ=255)
                                                              ccGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGt                                                                                         >  1:318694/1‑150 (MQ=255)
                                                              ccGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGt                                                                                         >  2:738315/1‑150 (MQ=255)
                                                              ccGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGt                                                                                         >  1:847990/1‑150 (MQ=255)
                                                                 cTATTGCCTAAGGTCGTAAGGCGGCAGACGGTATTATGTACTGGCGGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGGCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGTGCGTTTg                                                                                      <  1:341832/150‑1 (MQ=255)
                                                                 cTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGGGTCTGGCGAATCAAATTACTGACGAACTGCTGTTTGATTTCTGGCAAATCCGGAATGAGCGtttt                                                                                      >  2:1402687/1‑149 (MQ=255)
                                                                   aTTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGAc                                                                                    >  1:245398/1‑150 (MQ=255)
                                                                    ttGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGAcc                                                                                   >  2:1756207/1‑150 (MQ=255)
                                                                      gCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCtt                                                                                 >  1:1004111/1‑150 (MQ=255)
                                                                        cGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTc                                                                               <  2:1555224/150‑1 (MQ=255)
                                                                        cGAAGGGCGGAATGCGGGAGTCGGTATTTTGCACTGGCTGGAAGTTTAATCGATGTAACAATGAATTGGCCCTTATGTGTCTGGGGTATCAAAATACTGACGTAGTGCGCGGTGGTTTTTGGCAAATGCGGCATGAGCGTTTGACCTTTc                                                                               <  1:897838/150‑1 (MQ=255)
                                                                            ggTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCtat                                                                           >  1:978392/1‑150 (MQ=255)
                                                                               ggTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTataat                                                                        <  1:368496/149‑1 (MQ=255)
                                                                                gTAAGGAGGCAGACGGTATTATGAACTGTCTGGATGTTTAAGCGAGGTAACAATGAATTCGCCTTTCTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTataata                                                                       <  2:70145/150‑1 (MQ=255)
                                                                                  aaGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAgg                                                                     >  1:1802534/1‑150 (MQ=255)
                                                                                  aaGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAgg                                                                     <  2:1577559/150‑1 (MQ=255)
                                                                                   aGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAggg                                                                    <  2:1362413/150‑1 (MQ=255)
                                                                                    ggcggcAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGa                                                                   <  1:180647/150‑1 (MQ=255)
                                                                                    ggcggcAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGa                                                                   >  2:1442660/1‑150 (MQ=255)
                                                                                     gcggcAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGAt                                                                  >  2:24293/1‑150 (MQ=255)
                                                                                      cggcAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATa                                                                 >  2:1019055/1‑150 (MQ=255)
                                                                                       ggcAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATaa                                                                >  1:659905/1‑150 (MQ=255)
                                                                                         cAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTTAAGACGAACTGCGCGTTGTTTTATGGCAAATGCGGCATGAGCGTTTGACCTTTACTATAATAGGGATAAtt                                                              >  1:874360/1‑150 (MQ=255)
                                                                                         cAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAAtt                                                              >  1:1873620/1‑150 (MQ=255)
                                                                                           gACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAAtttt                                                            >  1:1589794/1‑150 (MQ=255)
                                                                                           gACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGATGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAAtttt                                                            >  1:1005484/1‑150 (MQ=255)
                                                                                            aCGTTATTATGAACTGTCTTGAAGTTTAAGAGAGTTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTc                                                           <  1:1313093/150‑1 (MQ=255)
                                                                                            aCGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTc                                                           <  1:738315/150‑1 (MQ=255)
                                                                                              ggTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCaa                                                         <  1:377413/150‑1 (MQ=255)
                                                                                                    atGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAAtt                                                   >  2:256688/1‑150 (MQ=255)
                                                                                                     tGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATtg                                                  <  1:534006/150‑1 (MQ=255)
                                                                                                      tatctggatggAAGTTTAAGCGAGGTCACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGCCGAAGTGCGCGGTGGTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATtgt                                                 <  1:891096/147‑1 (MQ=255)
                                                                                                      gAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATtgt                                                 >  2:659058/1‑150 (MQ=255)
                                                                                                       aaCTGTCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGGGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTATCAATAAATtgtg                                                <  2:76053/150‑1 (MQ=255)
                                                                                                        aCTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGc                                               <  1:1410862/150‑1 (MQ=255)
                                                                                                         ctggctggAAGTTTAAGCGAGTTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCt                                              <  2:1262560/150‑1 (MQ=255)
                                                                                                           ggctggAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCttt                                            >  1:1156195/1‑150 (MQ=255)
                                                                                                            gctggAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTg                                           <  1:449914/150‑1 (MQ=255)
                                                                                                             ctggAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGAAGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGt                                          >  2:1381165/1‑150 (MQ=255)
                                                                                                                gAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCa                                       <  1:1040825/150‑1 (MQ=255)
                                                                                                                 aaGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTCGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCAc                                      <  2:758775/150‑1 (MQ=255)
                                                                                                                    tttAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGACTCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCAcctc                                   >  1:312561/1‑148 (MQ=255)
                                                                                                                    tttAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTACTATAATAGGGATAATTTTCAATAAATTTTGCTTTGTTCACCaa                                   >  1:1393403/1‑150 (MQ=255)
                                                                                                                     ttAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAg                                  >  2:657271/1‑150 (MQ=255)
                                                                                                                       aaGCGAGGTAACAAAGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTc                                <  2:1659759/150‑1 (MQ=255)
                                                                                                                           gAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCtgat                            >  1:278165/1‑150 (MQ=255)
                                                                                                                            aGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCtgatg                           <  1:68644/150‑1 (MQ=255)
                                                                                                                            aGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCtgatg                           >  1:376577/1‑150 (MQ=255)
                                                                                                                               tAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAATTGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGt                        >  2:123620/1‑150 (MQ=255)
                                                                                                                                aaCAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTg                       <  2:96670/150‑1 (MQ=255)
                                                                                                                                aaCAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTg                       >  1:503678/1‑150 (MQ=255)
                                                                                                                                aaCAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTg                       <  1:350342/150‑1 (MQ=255)
                                                                                                                                aaCAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTg                       >  2:634470/1‑150 (MQ=255)
                                                                                                                                   aaTGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAg                    >  1:1001280/1‑150 (MQ=255)
                                                                                                                                      tgaTTCGCCAGACACAGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCATTAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGctc                 >  1:217146/3‑148 (MQ=255)
                                                                                                                                      tgaTTCGCCAGACACAGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGcac                 >  2:1761978/3‑150 (MQ=255)
                                                                                                                                      gAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGcac                 <  1:653512/150‑1 (MQ=255)
                                                                                                                                         ttCGCCAGACACAGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATc              >  2:131429/1‑150 (MQ=255)
                                                                                                                                         ttCGCCAGACACAGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATc              >  1:622037/1‑150 (MQ=255)
                                                                                                                                         ttCGCCAGACACAGTCTGGCGAATCAAATTACTGACGAAGTGAGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATc              >  2:1062991/1‑150 (MQ=255)
                                                                                                                                           cGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGTAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCaa            >  1:482538/1‑150 (MQ=255)
                                                                                                                                           cGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCaa            <  1:566094/150‑1 (MQ=255)
                                                                                                                                                 aCTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAGCAAtt      >  2:672035/1‑150 (MQ=255)
                                                                                                                                                   tgtgTATGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAGCAATTtc    <  1:1325936/150‑1 (MQ=255)
                                                                                                                                                     tgtCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAGCAATTtctc  >  2:1435021/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAGCAATTTCTC  >  NC_000913/3360496‑3360794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: