Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,371,069 G→T 5.6% intergenic (‑33/+15) nanE ← / ← nanT putative N‑acetylmannosamine‑6‑P epimerase/sialic acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,371,0690GT 5.6% 416.3 / 10.5 144intergenic (‑33/+15)nanE/nanTputative N‑acetylmannosamine‑6‑P epimerase/sialic acid transporter
Reads supporting (aligned to +/- strand):  ref base G (63/71);  new base T (5/4);  total (68/76)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 147 total aligned reads displayed.

TGCCATGGCGGCGACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATC  >  NC_000913/3370920‑3371218
                                                                                                                                                     |                                                                                                                                                     
tGCCATGGCGGCGACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCAGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAg                                                                                                                                                       <  2:1177832/150‑1 (MQ=255)
  ccATGGCGGCGACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGAc                                                                                                                                                     >  1:1463867/1‑150 (MQ=255)
       gcggcgACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGc                                                                                                                                                <  1:680176/150‑1 (MQ=255)
       gcggcgACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGc                                                                                                                                                <  2:703206/150‑1 (MQ=255)
       gcggcgACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGc                                                                                                                                                <  2:441359/150‑1 (MQ=255)
       gcggcgACGATTTCGGGTTTATCGAGCGGGCTGGCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGc                                                                                                                                                <  2:1508836/150‑1 (MQ=255)
        cggcgACGATTTCGGGCTTATCGAGCGGGCGGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTCTATGTACCGGGcc                                                                                                                                               <  2:1680619/150‑2 (MQ=255)
         ggcgACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTCTATGTACCGGGcct                                                                                                                                              <  1:1264338/150‑3 (MQ=255)
          gagacgaTTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCACGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAAc                                                                                                                                             <  1:1104314/148‑1 (MQ=255)
           cgacgaTTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACa                                                                                                                                            >  2:1828626/1‑150 (MQ=255)
            gacgaTTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAg                                                                                                                                           <  1:1490333/150‑1 (MQ=255)
             ccgaTTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGCGAGAATCCGGCCACCGTTAGGAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCATCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAgg                                                                                                                                          <  2:821349/149‑1 (MQ=255)
               tttttCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGATTAAATCAGGCCATCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGAt                                                                                                                                        <  2:813957/148‑1 (MQ=255)
               gaattCGGGTTTATCGAGCGGGCGGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGAt                                                                                                                                        <  1:1630193/147‑1 (MQ=255)
               gaTTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGAt                                                                                                                                        >  1:506157/1‑150 (MQ=255)
                   tCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAAc                                                                                                                                    <  1:388352/150‑1 (MQ=255)
                        tttATCGAGAGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTg                                                                                                                               <  2:1378518/150‑1 (MQ=255)
                         ttATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTgg                                                                                                                              <  1:510291/150‑1 (MQ=255)
                         ttATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTgg                                                                                                                              <  1:273590/150‑1 (MQ=255)
                           aTCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTATGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATATACCGGGCAACAGGATTAACTTTTTGtt                                                                                                                            >  1:911716/1‑150 (MQ=255)
                            tCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGttt                                                                                                                           >  1:914273/1‑150 (MQ=255)
                             cGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACTATTACGCCACCGTTTGCAGCTATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGTAACAGGATTAACTTTTGGtttt                                                                                                                          <  1:352802/150‑1 (MQ=255)
                             cGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGtttt                                                                                                                          >  2:171111/1‑150 (MQ=255)
                             cGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGtttt                                                                                                                          >  2:966534/1‑150 (MQ=255)
                              gAGCGGGCTGTCCGGAACCGGCTGGCTGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCATTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTg                                                                                                                         <  2:1221518/150‑1 (MQ=255)
                              gAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTg                                                                                                                         <  2:194676/150‑1 (MQ=255)
                               aGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGa                                                                                                                        >  2:805262/1‑150 (MQ=255)
                               aGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGa                                                                                                                        <  2:611602/150‑1 (MQ=255)
                                gCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGAc                                                                                                                       >  2:663023/1‑150 (MQ=255)
                                  gggCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTa                                                                                                                     >  1:1795763/1‑150 (MQ=255)
                                   ggCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCACCAGGATTAACTTTTGGTTTTGACTaa                                                                                                                    >  1:1151663/1‑150 (MQ=255)
                                   ggCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTATTGACTaa                                                                                                                    >  2:1282600/1‑150 (MQ=255)
                                         ccGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGtt                                                                                                              <  1:810424/150‑1 (MQ=255)
                                          cGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGttt                                                                                                             >  1:639414/1‑150 (MQ=255)
                                          cGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACAGAGACCGGGCAACAGGAGTAACTTTTGGTTTTGACTAAATCGttt                                                                                                             >  2:1748548/1‑150 (MQ=255)
                                           ggAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTGTTGGTTTTGACTAAATCGtttt                                                                                                            >  1:1068920/1‑150 (MQ=255)
                                            tatccGGATGGCAGGAGACAAACATGCCACCGTTAGAATCGATTTTTTGATCCATTTGTGCAAGTAACGACATTAATATTCCCTAATGGAAAGGCCCGGTACTTAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGttttt                                                                                                           <  1:138895/147‑1 (MQ=255)
                                             aaCCGGCTGGCAGTAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTg                                                                                                          <  1:1690431/150‑1 (MQ=255)
                                              aCCGGCTGGCAGGAGACACTCAGGCGACGGTTAGCAGCGATTTTTTGATCCAGTTGGGCAAGTAACGACATACATCTTCCCTTAGCTAAAGGCCCGGTACATAGACCTGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTgg                                                                                                         <  2:243856/150‑1 (MQ=255)
                                              aCCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTgg                                                                                                         <  2:1804406/150‑1 (MQ=255)
                                                cGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGgcg                                                                                                       <  2:803909/150‑1 (MQ=255)
                                                cGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGgcg                                                                                                       >  1:906758/1‑150 (MQ=255)
                                                 ggCTGGCAGGATACAATCAGGCCACCCTAAGCAGCGATATTTTGATCCAGTTCTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACAAAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGgcgc                                                                                                      <  2:1146558/150‑1 (MQ=255)
                                                    tGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGc                                                                                                   <  2:1763161/150‑1 (MQ=255)
                                                     ggCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGcc                                                                                                  <  1:1608752/150‑1 (MQ=255)
                                                     ggCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGcc                                                                                                  <  2:1545387/150‑1 (MQ=255)
                                                      gCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGATAGGCCCTGAACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCa                                                                                                 <  1:1826573/150‑1 (MQ=255)
                                                              cAATCAGGCAACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAacggcac                                                                                         >  1:1004550/1‑150 (MQ=255)
                                                                aTCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAacggcacgg                                                                                       <  1:1125402/150‑1 (MQ=255)
                                                                    ggCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCAcc                                                                                   <  1:789506/150‑1 (MQ=255)
                                                                    ggCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCAcc                                                                                   >  1:1009803/1‑150 (MQ=255)
                                                                       cACGGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTATCTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCt                                                                                >  2:950901/1‑150 (MQ=255)
                                                                           gTTAGCAGCGATTTTTTGATCCAGTTGTGTAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAAt                                                                            <  1:867710/150‑1 (MQ=255)
                                                                             tAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCGCCGCTGAATgg                                                                          <  2:740601/150‑1 (MQ=255)
                                                                              agcagtTATTTTTTGATCCAGTTGTGCATGTAACGACCTACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGt                                                                         <  1:295189/150‑1 (MQ=255)
                                                                                cagcGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACGGATGAATGGttt                                                                       >  2:1154247/1‑150 (MQ=255)
                                                                                 agcGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTa                                                                      >  2:744149/1‑150 (MQ=255)
                                                                                  gcGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTAc                                                                     >  2:375782/1‑150 (MQ=255)
                                                                                    gaatttttGATCCAGTTGTGCAACTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCg                                                                   <  2:138629/147‑1 (MQ=255)
                                                                                    gATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCg                                                                   >  1:545700/1‑150 (MQ=255)
                                                                                    gATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCg                                                                   >  1:552883/1‑150 (MQ=255)
                                                                                    gATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACATTTGGTTTTGACTAAATCGTTTTTGGCGCTGCAAAACGGCACGGCACCGCTGAATGTTTTACCg                                                                   >  2:171349/1‑150 (MQ=255)
                                                                                            gATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGc                                                           >  1:1272467/1‑150 (MQ=255)
                                                                                                cAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCa                                                       <  2:235210/150‑1 (MQ=255)
                                                                                                cAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCa                                                       <  1:1486888/150‑1 (MQ=255)
                                                                                                  gTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATg                                                     <  1:218695/150‑1 (MQ=255)
                                                                                                  gTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATg                                                     <  2:197347/150‑1 (MQ=255)
                                                                                                     gtgCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGt                                                  >  1:445126/1‑150 (MQ=255)
                                                                                                      tgCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTa                                                 <  1:1290163/150‑1 (MQ=255)
                                                                                                        cAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACg                                               >  2:1045607/1‑150 (MQ=255)
                                                                                                          aGTAACGACATACATCTTCCCTTAGCTAAAGGCCCGGTACATAGAAAGGGAAACAGGATTAACTTTTGGTTTTCACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACAGTCGATAGCGTCATGAGTACGCa                                             >  2:1550042/1‑150 (MQ=255)
                                                                                                           gTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCaa                                            >  2:609731/1‑150 (MQ=255)
                                                                                                                  catacatCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTATCTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTcc                                     >  2:1666489/1‑150 (MQ=255)
                                                                                                                        gctacccTTCGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGCTTTGACTAAATCGTTTTTGGCGCGGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGgcgc                               <  1:1699296/146‑1 (MQ=255)
                                                                                                                         cTTCCCTTATCGTAAGGCCAGGTACATAGACCGGGCAAGAGGATTAACGTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCATACGGCACGGCACCGCTGAATTGCTTACCGTCGATAGCGTCATGAGTTCGCAACGCTTCCGGGCGca                              <  1:955176/150‑1 (MQ=255)
                                                                                                                            cccTAATCGAAAGGCCCGGTACATAGACCTGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGGTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGcaacc                           <  2:353602/150‑1 (MQ=255)
                                                                                                                              cTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGGTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGGCATGAGTACGCAACGCTTCCGGGCGcaaccaa                         >  1:1137417/1‑150 (MQ=255)
                                                                                                                               ttAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGcaaccaac                        <  1:663023/150‑1 (MQ=255)
                                                                                                                               ttAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGcaaccaac                        <  1:968812/150‑1 (MQ=255)
                                                                                                                                 aGCGAAAGGCCCGGTACATAGACCGGGCAACAGTATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGc                      <  1:1131650/150‑1 (MQ=255)
                                                                                                                                  gCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACGTTTGGTTTTGACTAAATCGTTTTTGGCGCTGACAACCGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGATTAAGCAACGATTCCGGGCGCAAACAACGCt                     >  1:1235503/1‑150 (MQ=255)
                                                                                                                                   cGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTg                    >  1:1562161/1‑150 (MQ=255)
                                                                                                                                   cGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTATAGAGTACTCAACGCTTCCGGGCGCAACCAACGCTg                    >  1:1562914/1‑150 (MQ=255)
                                                                                                                                   cGAAAGGCCCGGTACATACCCCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTg                    <  2:1154124/150‑1 (MQ=255)
                                                                                                                                    gAAAGGCGCGGTACATAGACCGGGCAACAGGATTAAAGTTTGGTTTTGACTAAATCGTTTTTGGCTCTGCCAAACGGCACGGCACCGCTGAATGGTTTTCCGTCGATAGCGTCATGATTACGCAACGCTTCCGGGCGCAACCAACGCTGa                   <  1:85823/150‑1 (MQ=255)
                                                                                                                                    ctgttgCCCGGTCTATGTACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGATTCCGGGCGCAACCAACGCTGa                   >  2:1173588/6‑150 (MQ=255)
                                                                                                                                     tgttgCCCGGTCTATGTACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGaa                  >  1:437625/5‑150 (MQ=255)
                                                                                                                                      gttgCCCGGTCTATGTACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAAc                 >  1:619187/4‑150 (MQ=255)
                                                                                                                                      aaGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAAc                 <  1:699714/150‑1 (MQ=255)
                                                                                                                                         gCCCGGTCTATGTACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTa                                                                      <  2:883349/94‑1 (MQ=255)
                                                                                                                                         gCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAAcgcg              <  1:232261/150‑1 (MQ=255)
                                                                                                                                         ccccGGTACATCGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCAGCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAAcgcg              <  2:485349/149‑1 (MQ=255)
                                                                                                                                          cccGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCga             >  1:334423/1‑150 (MQ=255)
                                                                                                                                          cccGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCga             >  1:1409308/1‑150 (MQ=255)
                                                                                                                                            cGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCgaga           <  1:585220/150‑1 (MQ=255)
                                                                                                                                             ggTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGaa          <  2:1132888/150‑1 (MQ=255)
                                                                                                                                                 cATATACCGGGCAACAGGATTAACTTTTGGTTTAGAATAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTCCCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCa      <  2:911716/150‑1 (MQ=255)
                                                                                                                                                 cATAGACCGGGCAACAGGATTATCTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTGCGGGCGCAACCAACGCTGAACGCGAGAAGGCa      <  1:950901/150‑1 (MQ=255)
                                                                                                                                                 cATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCa      >  2:522309/1‑150 (MQ=255)
                                                                                                                                                  aTAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTCAGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCat     >  1:1104566/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCCATGGCGGCGACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATC  >  NC_000913/3370920‑3371218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: