Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,418,629 T→C 6.5% intergenic (+18/‑413) yhdV → / → yhdW putative outer membrane protein/pseudogene, ABC transporter periplasmic binding protein family;putative transport; Not classified; putative periplasmic binding transport protein; periplasmic‑binding component of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,418,6290TC 6.5% 449.3 / 16.3 156intergenic (+18/‑413)yhdV/yhdWputative outer membrane protein/pseudogene, ABC transporter periplasmic binding protein family;putative transport; Not classified; putative periplasmic binding transport protein; periplasmic‑binding component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base T (76/68);  new base C (5/5);  total (82/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-02

Only 100 of 161 total aligned reads displayed.

GGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTCACACTTCCCAC  >  NC_000913/3418480‑3418778
                                                                                                                                                     |                                                                                                                                                     
ggTCGTATGGTTCACACCAATACCTGTATGACATGCACGACTCACGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGt                                                                                                                                                       <  1:882085/150‑1 (MQ=255)
    gtcTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCcg                                                                                                                                                   <  1:1685773/150‑1 (MQ=255)
     tcTGGTTCACACCAATGCCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCcgg                                                                                                                                                  >  2:149775/1‑149 (MQ=255)
     tcTGGTTCACACCAATGCCTGTATGAAAGGAACGACTAAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGGGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAAAAACTGAACAGGGCGCGGAGTTCcgg                                                                                                                                                  >  2:149778/1‑149 (MQ=255)
      cTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCcgcg                                                                                                                                                 <  1:61462/150‑1 (MQ=255)
      cTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCcgcg                                                                                                                                                 >  2:428294/1‑150 (MQ=255)
       tGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCgcgc                                                                                                                                                <  2:846282/150‑1 (MQ=255)
         gTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAACTCCgcgccc                                                                                                                                              <  1:443250/150‑3 (MQ=255)
          ttCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGctct                                                                                                                                             >  1:750896/1‑150 (MQ=255)
           tCACACCAATACCTGTTTGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAACTCCGCGccctg                                                                                                                                            <  1:1260750/150‑2 (MQ=255)
           tCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCtt                                                                                                                                            <  1:188202/150‑1 (MQ=255)
            cacaCCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCttt                                                                                                                                           >  2:961612/1‑150 (MQ=255)
            cacaCCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCGGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAACTCCGCGccctgt                                                                                                                                           <  1:729979/150‑3 (MQ=255)
              cacCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGGGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCttttt                                                                                                                                         >  1:608339/1‑150 (MQ=255)
              cacCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCttttt                                                                                                                                         <  2:1622474/150‑1 (MQ=255)
              cacCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCttttt                                                                                                                                         <  1:1825102/150‑1 (MQ=255)
              cacCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCttttt                                                                                                                                         >  2:1224126/1‑150 (MQ=255)
              cacCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCttttt                                                                                                                                         >  1:443931/1‑150 (MQ=255)
                ccAATACCTGTATGAAAGGCACGACTCAGGATATCTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATGATCGCTCTATCGAAATAACTGAACAGAGCGCGGATCTCCGCGCCCtgttcag                                                                                                                                       <  1:1469049/150‑4 (MQ=255)
                ccAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCAGTAGCTGGCATGACGATGGGCATCATCGCTCTATCGATATAACTGAACAGGGCGCCGAGTTCCGCGCTCTTTATaa                                                                                                                                       >  1:1334346/1‑150 (MQ=255)
                 cAATACCTGTATGAAAGGCACGACGCAGGATAACTGGGAAACGGCTGGGGATATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAAc                                                                                                                                      <  2:626006/150‑1 (MQ=255)
                  aaTACCTGTATGAACGGCACGACGCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACg                                                                                                                                     <  1:1415150/150‑1 (MQ=255)
                      ccTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATAc                                                                                                                                 >  1:101928/1‑150 (MQ=255)
                       gtGTATGAAAGGCACGACTCAGGATAACTGGGAATCGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAATTAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACa                                                                                                                                <  1:577803/149‑1 (MQ=255)
                       cTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACa                                                                                                                                <  1:969320/150‑1 (MQ=255)
                         gTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGt                                                                                                                              >  2:1559449/1‑150 (MQ=255)
                         gTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAAGAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGt                                                                                                                              >  1:1383857/1‑150 (MQ=255)
                          tATGAAAGGCACGACTCAGCATAACTGGGAAACGGCTGGCGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGTCCTGACGATGGGTATCAACGCTATATGGAAAGAACTGAACAGGGCGCGGAGATCCGCGCTCTATTTAACGATACAGTa                                                                                                                             >  2:159112/1‑150 (MQ=255)
                             gAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTAtct                                                                                                                          >  2:934845/1‑150 (MQ=255)
                               acggCACGCCTCAGGCTAACTGGGATACGGCGGGGGCTATTGCCGGCGGGGCAGCTGCGGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTAtctct                                                                                                                        <  1:1083520/148‑1 (MQ=255)
                               acggCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCGCTTTTTAACGATACAGTAtctct                                                                                                                        <  1:1090329/148‑1 (MQ=255)
                                aGGCACGACTCAGGATAACTGGGAAACGGCGGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTAtctctc                                                                                                                       <  2:964667/150‑1 (MQ=255)
                                 cgCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTcc                                                                                                                      <  2:420424/149‑1 (MQ=255)
                                  gCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCa                                                                                                                     >  2:425523/1‑150 (MQ=255)
                                  gCACGACGCAGGATAACTGGGAAACCGCTGGGGCGATTGCCGGCGGGGCAGCGGCGGTTGCTGGCCGGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCa                                                                                                                     <  2:1249921/150‑1 (MQ=255)
                                     cGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATaa                                                                                                                  <  2:660651/150‑1 (MQ=255)
                                          cAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGTATCATCGCTCTATGGCAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGAAACAGTATCTCTCCATAAGATaa                                                                                                             <  2:1017813/150‑1 (MQ=255)
                                          cAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATaa                                                                                                             >  1:1750198/1‑150 (MQ=255)
                                           aGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATaaa                                                                                                            >  2:1667388/1‑150 (MQ=255)
                                                  cTGGGCAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCGGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACATGGCGCGGAGTTCCGCGCGCTTTTTAACGATACAGTATCTCTCCTTAAGATAAAATATTGc                                                                                                     <  1:237373/150‑1 (MQ=255)
                                                  cTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGc                                                                                                     <  1:296087/150‑1 (MQ=255)
                                                  cTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGc                                                                                                     <  2:31520/150‑1 (MQ=255)
                                                        aaCGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATAtt                                                                                               >  2:650042/1‑150 (MQ=255)
                                                           ggCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTctg                                                                                            <  2:1426899/150‑1 (MQ=255)
                                                               ggggCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTgg                                                                                        <  1:413145/150‑1 (MQ=255)
                                                                gggCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTggg                                                                                       <  2:1299296/150‑1 (MQ=255)
                                                                  gCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTa                                                                                     >  1:659572/1‑150 (MQ=255)
                                                                   gtATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCGGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTaa                                                                                    <  2:409983/149‑1 (MQ=255)
                                                                    tATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAAt                                                                                   >  1:1006041/1‑150 (MQ=255)
                                                                      ttGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTc                                                                                 <  1:1359078/150‑1 (MQ=255)
                                                                           ggcggGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCctgc                                                                            >  2:1757199/1‑150 (MQ=255)
                                                                           ggcggGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCctgc                                                                            >  1:1608985/1‑150 (MQ=255)
                                                                             cggGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCctgctg                                                                          >  2:1650604/1‑150 (MQ=255)
                                                                                 gCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTTACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGAttt                                                                      <  1:1678047/150‑1 (MQ=255)
                                                                                  cAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTa                                                                     <  2:555146/150‑1 (MQ=255)
                                                                                   aGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGCTACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTACCTGCTGATTTAg                                                                    >  1:90295/1‑150 (MQ=255)
                                                                                     ctgctgTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCa                                                                  <  1:1750637/150‑1 (MQ=255)
                                                                                          gTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTa                                                             <  1:961612/150‑1 (MQ=255)
                                                                                            tGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACa                                                           >  1:1295592/1‑150 (MQ=255)
                                                                                             gCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACAt                                                          <  2:101928/150‑1 (MQ=255)
                                                                                              cTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCGCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATc                                                         <  1:1310256/150‑1 (MQ=255)
                                                                                                gtacTGACTATGGGTATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTATACATAAGATAAAATATTGCTATATTATGCTGTGTAATTAACTGCTCATTTAGCATGTTACAtatc                                                       >  2:761791/4‑147 (MQ=255)
                                                                                                ggCCTGACGGTGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGATATATTCTGCTGGGTAATTCACTGCTGATTTAGCATGTTACATcgc                                                       >  2:614047/1‑150 (MQ=255)
                                                                                                ggCCTCACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGCAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTTCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATcgc                                                       >  2:853218/1‑150 (MQ=255)
                                                                                                 gCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATcgcg                                                      <  2:1801711/150‑1 (MQ=255)
                                                                                                    tGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTa                                                   >  2:1682247/1‑150 (MQ=255)
                                                                                                       cGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTAttt                                                >  2:468152/1‑150 (MQ=255)
                                                                                                         ttGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCg                                              <  1:124002/149‑1 (MQ=255)
                                                                                                           ggggATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCgct                                            <  1:724907/150‑1 (MQ=255)
                                                                                                            gggATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATCTCgctg                                           <  2:1445126/150‑1 (MQ=255)
                                                                                                             ggATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCgctgc                                          >  2:407301/1‑150 (MQ=255)
                                                                                                             ggATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTACGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCACTGCTGATTTAGCATGTTACATCGCGTTATTTCgctgc                                          >  2:32014/1‑150 (MQ=255)
                                                                                                                tcatcGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCttt                                       <  2:1002322/150‑1 (MQ=255)
                                                                                                                  atcGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCg                                     >  1:1255334/1‑150 (MQ=255)
                                                                                                                         tATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCACTGCTGATTTAGCATGATACATCGCGTTATTTCGCTGCTTTCGAAATAAc                              >  1:1194754/1‑150 (MQ=255)
                                                                                                                            cGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCa                           >  1:736287/1‑150 (MQ=255)
                                                                                                                             gAACTAACTGAACAGGGCGGGGAGTTCAGCGCTCTTTTTAACGATACAGTATCTCTCCTTAAGATAAAATATTGCTATATGCTGCTGGGTAATTCCCTGCGGATTTTGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCAt                          <  2:280212/150‑1 (MQ=255)
                                                                                                                             gAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCAt                          >  1:956662/1‑150 (MQ=255)
                                                                                                                             gAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAAAGATACACTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTTATTCCATGGAGATTTAGCAAGTTACATCGAGTTTTTTCGCTGCTTTCGAAATAACGCAt                          >  2:1166965/1‑150 (MQ=255)
                                                                                                                                    cTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCCTCaattta                   >  2:1370645/1‑150 (MQ=255)
                                                                                                                                    cTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCaattta                   >  1:472642/1‑150 (MQ=255)
                                                                                                                                    cTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCaattta                   >  1:540643/1‑150 (MQ=255)
                                                                                                                                      gAACAGGGCGAGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTATAAATAACGCATCaatttaat                 <  2:680149/150‑1 (MQ=255)
                                                                                                                                      aaaaagagcgcGGAACTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCaatttaat                 >  2:278018/8‑150 (MQ=255)
                                                                                                                                      aaaaagagcgcGGAACTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCaatttaat                 >  1:949143/8‑150 (MQ=255)
                                                                                                                                        aCAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCaatttaattt               <  2:1151818/150‑1 (MQ=255)
                                                                                                                                        aCAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCaatttaattt               <  1:84849/150‑1 (MQ=255)
                                                                                                                                          cgggCGCCGAGGTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTc             <  1:149775/149‑1 (MQ=255)
                                                                                                                                          atggCGCGGATTTCCGCTCTATTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGCGTAATTCCCTTCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTc             <  1:149778/148‑1 (MQ=255)
                                                                                                                                          agagcgcGGAACTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTc             >  1:1171985/4‑150 (MQ=255)
                                                                                                                                          agagcgcGGAACTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATAAATTTAATTTTc             >  2:1690976/4‑150 (MQ=255)
                                                                                                                                          aGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTc             >  2:537351/1‑150 (MQ=255)
                                                                                                                                            ggCGCGGAGTTCCGCGCGCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTTTATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGGTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTcac           <  1:1596545/150‑1 (MQ=255)
                                                                                                                                             gcgcGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTGGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTcaca          >  1:1159144/1‑150 (MQ=255)
                                                                                                                                              cgcgGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTCacac         >  2:1770583/1‑150 (MQ=255)
                                                                                                                                                   aGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTCACACTTccc    <  2:20867/150‑1 (MQ=255)
                                                                                                                                                   aGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTCACACTTccc    <  2:1083622/150‑1 (MQ=255)
                                                                                                                                                    gTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTCACACTTCcca   >  1:374213/1‑150 (MQ=255)
                                                                                                                                                     ttCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTCACACTTCccac  <  1:1667388/150‑1 (MQ=255)
                                                                                                                                                     ttCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTCACACTTCccac  <  1:1527660/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTCACACTTCCCAC  >  NC_000913/3418480‑3418778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: