Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,437,909 A→T 5.3% intergenic (+15/‑115) trkA → / → mscL NAD‑binding component of TrK potassium transporter/mechanosensitive channel protein, high conductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,437,9090AT 5.3% 387.6 / 7.2 128intergenic (+15/‑115)trkA/mscLNAD‑binding component of TrK potassium transporter/mechanosensitive channel protein, high conductance
Reads supporting (aligned to +/- strand):  ref base A (52/68);  new base T (4/3);  total (57/71)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 130 total aligned reads displayed.

GTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGA  >  NC_000913/3437761‑3438057
                                                                                                                                                    |                                                                                                                                                    
gTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTaa                                                                                                                                                     >  2:519968/1‑150 (MQ=255)
gTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTaa                                                                                                                                                     >  1:1387832/1‑150 (MQ=255)
   gAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGa                                                                                                                                                  >  1:466735/1‑150 (MQ=255)
     aaCGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACg                                                                                                                                                <  1:823079/150‑1 (MQ=255)
         acgTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCtt                                                                                                                                            <  2:893749/150‑1 (MQ=255)
         acgGGATGATTGCCAATGAGAATCTGCGCATTGAGCAAGGCGATCTAGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCtt                                                                                                                                            <  2:893752/150‑1 (MQ=255)
          cgTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACTTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCtta                                                                                                                                           <  2:1667397/150‑1 (MQ=255)
           gTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttat                                                                                                                                          <  1:142146/150‑1 (MQ=255)
            tgatgaTTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatt                                                                                                                                         <  2:1242432/150‑1 (MQ=255)
            tgatgaTTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatt                                                                                                                                         <  2:1446915/150‑1 (MQ=255)
            tgatgaTTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatt                                                                                                                                         <  1:962637/150‑1 (MQ=255)
            tgatgaTTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACACTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatt                                                                                                                                         <  2:391329/150‑1 (MQ=255)
             gatgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatta                                                                                                                                        >  2:1765309/1‑150 (MQ=255)
             gatgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTACAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatta                                                                                                                                        <  1:533399/150‑1 (MQ=255)
                gatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTAttt                                                                                                                                     >  1:1584417/1‑150 (MQ=255)
                 atTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTc                                                                                                                                    <  2:1827749/150‑1 (MQ=255)
                 atTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTc                                                                                                                                    <  1:735413/150‑1 (MQ=255)
                  ttGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCATCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTcc                                                                                                                                   <  1:1040490/150‑1 (MQ=255)
                    gCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGCCGTCGAAAGACTCTTCCAGCCAAGCCCTTTATTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTcccg                                                                                                                                 >  1:169208/1‑149 (MQ=255)
                    gCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCt                                                                                                                                 >  1:955252/1‑150 (MQ=255)
                     ccAATGACAATCTGCTCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCtt                                                                                                                                <  2:1315469/150‑1 (MQ=255)
                        aTGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGa                                                                                                                             <  1:429982/150‑1 (MQ=255)
                        aTGACAATCTGCGCATAGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTATAAATAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGa                                                                                                                             <  1:271166/150‑1 (MQ=255)
                                cTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAg                                                                                                                     <  2:1559524/150‑1 (MQ=255)
                                 tGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAgg                                                                                                                    <  1:922936/150‑1 (MQ=255)
                                    gcATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGatt                                                                                                                 <  2:1111849/150‑1 (MQ=255)
                                    gcATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGatt                                                                                                                 <  2:1326446/150‑1 (MQ=255)
                                     cATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGatta                                                                                                                >  1:878583/1‑150 (MQ=255)
                                     cATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTATAATTAATAAGGCGTCTAATGACGCCTTATTTTTTCCCTTTGATTATCAAGGatta                                                                                                                >  2:1688335/1‑150 (MQ=255)
                                        tGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaatt                                                                                                             <  1:1495705/150‑1 (MQ=255)
                                        tGAGCAAGGCGATCACGTAATTATGTTCCTCAAAGATAAAAAGTTTATTACCTACGTCGAAAGACTCTTCCAGCCAAGCCCTTTATTCTTGTAATTAATAAGGCTTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaatt                                                                                                             <  1:442438/150‑1 (MQ=255)
                                          aGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGAttaattaa                                                                                                           >  1:767694/1‑150 (MQ=255)
                                           gCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTaaa                                                                                                          >  2:1734684/1‑150 (MQ=255)
                                           gCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTaaa                                                                                                          >  1:1047485/1‑150 (MQ=255)
                                            cAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAat                                                                                                         <  1:1713019/150‑1 (MQ=255)
                                              aGGCTATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACAGAAGTCGAACGACTATTCCAGCCAATCTATTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAattc                                                                                                       <  1:172423/150‑1 (MQ=255)
                                                 cGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAattcatt                                                                                                    >  2:1581257/1‑150 (MQ=255)
                                                   aTCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTcc                                                                                                  >  2:1099714/1‑150 (MQ=255)
                                                     cACGTAATTATTTTCCTCACATATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCATGCCCATTCATCTTGTAATAAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTAACAAGGATTAATTAAATTCATTCCTg                                                                                                <  2:175797/150‑1 (MQ=255)
                                                     cACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTg                                                                                                <  2:1450135/150‑1 (MQ=255)
                                                      aCGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCGTgg                                                                                               >  2:102538/1‑150 (MQ=255)
                                                       cGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGc                                                                                              >  1:1466663/1‑150 (MQ=255)
                                                         tAATTATGTTCCTCACAGATTAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAg                                                                                            <  1:942793/150‑1 (MQ=255)
                                                         tAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAg                                                                                            <  1:618228/150‑1 (MQ=255)
                                                           aTTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGa                                                                                          <  1:1246944/150‑1 (MQ=255)
                                                               tGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAAATACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTAATTCCTGGCAGGAAAAt                                                                                      >  2:1298211/1‑150 (MQ=255)
                                                                gTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATg                                                                                     <  2:1384396/150‑1 (MQ=255)
                                                                gTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCATTTATTCTTGAAATTAATAAGGCGTCTAATGACCCCTTATTAATTCCCTTTGATTATCAAGGATTAAATAAATACATTCCTGGCATGAAAAta                                                                                     >  2:840567/1‑149 (MQ=255)
                                                                    cTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCATCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCtt                                                                                 <  2:351869/150‑1 (MQ=255)
                                                                    cTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCtt                                                                                 >  2:678770/1‑150 (MQ=255)
                                                                    cTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCATGGCAGGAAAATGGCtt                                                                                 >  2:1717163/1‑150 (MQ=255)
                                                                      cacaGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTaa                                                                               <  2:1387832/150‑1 (MQ=255)
                                                                             aaaaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGt                                                                        <  2:1106665/150‑1 (MQ=255)
                                                                               aaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTa                                                                      >  2:1750054/1‑150 (MQ=255)
                                                                               aaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTACAGGCAGGAAAAAGGCTTAACATTTGTTa                                                                      >  2:1569158/1‑150 (MQ=255)
                                                                                aaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAg                                                                     <  1:1524675/150‑1 (MQ=255)
                                                                                  gTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTACTGGCAGGAAAATGGCTTAACATTTGTTAGAc                                                                   >  1:1659372/1‑150 (MQ=255)
                                                                                   tttATTACCGAAGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTAGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACt                                                                  <  2:697401/150‑1 (MQ=255)
                                                                                     tattaCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTa                                                                <  1:363218/150‑1 (MQ=255)
                                                                                        taCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATgg                                                             <  2:466735/150‑1 (MQ=255)
                                                                                         aCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGt                                                            <  1:689422/150‑1 (MQ=255)
                                                                                            gACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGt                                                         <  1:1066731/150‑1 (MQ=255)
                                                                                             aCGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTc                                                        <  2:1075321/150‑1 (MQ=255)
                                                                                              cGTCGAAAGACTCTTCCAGCCATGCCGTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCg                                                       >  2:1287766/1‑150 (MQ=255)
                                                                                               gTCGAAAGACTCTTCCAGCCACGCCGTTTGTTCTTGTAATTAATAAGGCGTCTAATGACGCCGGATTAGTTCGCTTTGATTATCTAGGATTAATTATATTCATTCCTGGCTGGATAATGGCTGACCATGTGTGTGCCTAATGGTTGTggg                                                      >  2:913355/1‑150 (MQ=255)
                                                                                                tCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGc                                                     >  1:775403/1‑150 (MQ=255)
                                                                                                 cGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCATGAAAATGGCGTAACAATTGTTAGACTTATGGTTGTCGGCt                                                    >  1:310129/1‑150 (MQ=255)
                                                                                                 cGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTAGGCt                                                    >  1:452847/1‑150 (MQ=255)
                                                                                                   aaaTACTCTTCAATCCAATACCTTTATTCTTGTAAATAATAATGCGTTTAATGTCGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTc                                                  <  1:1284521/150‑1 (MQ=255)
                                                                                                   aaaGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTc                                                  <  2:705482/150‑1 (MQ=255)
                                                                                                    aaGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCa                                                 <  2:828624/150‑1 (MQ=255)
                                                                                                       aCTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAATGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCATCGATTAATTAAATTCATAGCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCTGCTTCATat                                              >  2:641756/1‑149 (MQ=255)
                                                                                                       aCTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAg                                              <  1:650212/150‑1 (MQ=255)
                                                                                                       aCTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAg                                              <  2:354476/150‑1 (MQ=255)
                                                                                                         tctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATAATTTCCCTTTGATTATCAAGGATTTATTAAATTCATTCATGGCAGGAAAATGCCTTAACATTTGTTAGACTTATGGTTGTCGGATTCATAggt                                            >  1:40852/1‑149 (MQ=255)
                                                                                                          ctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGga                                           >  1:1373045/1‑150 (MQ=255)
                                                                                                           ttCCAGCCAAGACCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGgag                                          <  2:502679/150‑1 (MQ=255)
                                                                                                            tCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGgaga                                         <  1:130920/150‑1 (MQ=255)
                                                                                                              cAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAAt                                       <  2:1801569/150‑1 (MQ=255)
                                                                                                                  cAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACTCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACa                                   <  2:598556/150‑1 (MQ=255)
                                                                                                                      cccTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAg                               <  2:481783/150‑1 (MQ=255)
                                                                                                                       ccTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGc                              >  1:1296508/1‑150 (MQ=255)
                                                                                                                        cTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCa                             <  1:691463/150‑1 (MQ=255)
                                                                                                                         tttCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCat                            <  1:213633/150‑1 (MQ=255)
                                                                                                                             ttcttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCattatt                        >  1:1197948/1‑150 (MQ=255)
                                                                                                                              tcttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGATAGACTTATGGTTGTCGGCTTCATTGGGAGAATAACATGAGCAatatta                       >  2:706486/1‑150 (MQ=255)
                                                                                                                               cttGTAATTAATAAGGCGTCTAATGCCGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGCGCATTATTaa                      >  1:1228834/1‑150 (MQ=255)
                                                                                                                               cttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTTTCAAGGATAAATTAAATTCATTACTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTaa                      <  2:38318/150‑1 (MQ=255)
                                                                                                                                    aattaatAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAAt                 >  2:1726337/1‑150 (MQ=255)
                                                                                                                                     attaatAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAAtt                >  2:1132191/1‑150 (MQ=255)
                                                                                                                                       taataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAAttta              >  1:663195/1‑149 (MQ=255)
                                                                                                                                         ataaTGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgc            <  1:1221645/150‑1 (MQ=255)
                                                                                                                                         ataaGTCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgc            <  1:1595708/150‑1 (MQ=255)
                                                                                                                                         ataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgc            >  1:1618606/1‑150 (MQ=255)
                                                                                                                                         ataaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGTGCATTATTAAAGAATTTcga            >  1:929403/1‑149 (MQ=255)
                                                                                                                                          taaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAATGATTAATTAAATTCATTCCTGTCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgcg           <  2:807799/150‑1 (MQ=255)
                                                                                                                                           aaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGa          <  2:1440652/150‑1 (MQ=255)
                                                                                                                                             ggCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAAt        >  1:1456846/1‑150 (MQ=255)
                                                                                                                                                  cTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCg   <  2:1047485/150‑1 (MQ=255)
                                                                                                                                                   tAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGa  <  1:399840/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGA  >  NC_000913/3437761‑3438057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: