Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,441,061 G→C 5.5% S205S (TCC→TCG) ‡ rpsD ← 30S ribosomal subunit protein S4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,441,0610GC 5.5% 494.1 / 15.7 158S205S (TCC→TCG) ‡rpsD30S ribosomal subunit protein S4
Reads supporting (aligned to +/- strand):  ref base G (78/69);  new base C (6/3);  total (84/74)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01

Only 100 of 165 total aligned reads displayed.

CCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGCT  >  NC_000913/3440912‑3441208
                                                                                                                                                     |                                                                                                                                                   
ccAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTg                                                                                                                                                     <  1:1624664/150‑1 (MQ=255)
ccAGAGTAAGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTg                                                                                                                                                     <  2:415294/150‑1 (MQ=255)
 cAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTgg                                                                                                                                                    <  2:584565/150‑1 (MQ=255)
 cAGAGGATGGCCCACGCCACGATCTAAAGGCGCAAGGGGCACCGTGGCGGGCGTCGAACTCACTTGCGCGATATCAACCAGGCGCGGGTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTgg                                                                                                                                                    <  2:1169921/150‑1 (MQ=255)
  agagTATGGCCAAAGCCACGCTCTAACGGCTCAAGTGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGa                                                                                                                                                   <  2:806426/150‑1 (MQ=255)
   gagTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAg                                                                                                                                                  <  2:1536049/150‑1 (MQ=255)
   gagTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAg                                                                                                                                                  <  2:1651980/150‑1 (MQ=255)
   gagTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACATTGGCGTGCGTCGAACTCACTAGCTCGATATCAACCAGGCGCGGTGTTAGAAACACTGTCACAGAACCCTGCATTGTGGCCTCTCTTTGGTACTAAGCTTTACTAGGAg                                                                                                                                                  >  1:303800/1‑150 (MQ=255)
    agTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGGCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGt                                                                                                                                                 <  2:982227/150‑1 (MQ=255)
        tGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAg                                                                                                                                             <  2:1105617/150‑1 (MQ=255)
         ggCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGCACCCTGCATTGTGTCCTCACGTTGGTACTAAGCTTTACTTGGAGTAAAGc                                                                                                                                            >  1:1630112/1‑150 (MQ=255)
         ggCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGc                                                                                                                                            >  1:1473365/1‑150 (MQ=255)
             aaaGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGTCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCttag                                                                                                                                        <  2:1412447/150‑4 (MQ=255)
              aaGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTcgac                                                                                                                                       >  2:615734/1‑150 (MQ=255)
              aaGCCAAGCTCTTAAGGCTCCATGGTTCCCTTTGCGGGGGTCGAACCCACTTGCCCGCTATGAACCCGGCCGCGTTTTAGAAACCCTGGCACAGTACCCCGCATTTTGTTCCCTCTTTGGTACTAAGCTTTACTCCAAGTAAAggttagt                                                                                                                                       <  2:1472875/150‑7 (MQ=255)
              aaGCCAAGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTAGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTcgac                                                                                                                                       <  2:1804753/150‑1 (MQ=255)
               aGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTCCAAGTAAAGCttagta                                                                                                                                      <  1:259656/150‑6 (MQ=255)
                 ccACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGAt                                                                                                                                    >  1:633588/1‑150 (MQ=255)
                    cGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAg                                                                                                                                 <  1:534988/150‑1 (MQ=255)
                      ctctAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGgt                                                                                                                               >  1:1303884/1‑150 (MQ=255)
                          aaaGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGTAGTAAAGCTCGACGATCAGGTGTTc                                                                                                                           >  1:329955/1‑150 (MQ=255)
                          aaaGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTc                                                                                                                           >  1:233182/1‑150 (MQ=255)
                            aGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGTTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGt                                                                                                                         <  1:1060434/150‑1 (MQ=255)
                             ggCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGtt                                                                                                                        <  1:330343/150‑1 (MQ=255)
                              gCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTa                                                                                                                       >  1:686409/1‑150 (MQ=255)
                              gCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCGCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCTACGATCAGGTGGTCGTTa                                                                                                                       >  2:1489986/1‑150 (MQ=255)
                               cTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTaa                                                                                                                      <  1:1722620/150‑1 (MQ=255)
                               cTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTaa                                                                                                                      >  1:1009122/1‑150 (MQ=255)
                               cTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTaa                                                                                                                      <  1:1259096/150‑1 (MQ=255)
                                tCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAACGCACGACGATCAGGTGTTCGTTatt                                                                                                                     >  1:137851/1‑148 (MQ=255)
                                  aaGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGATGATCAGGTGTTCGTTAATGt                                                                                                                   <  1:1041542/150‑1 (MQ=255)
                                    gggTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCAGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTcc                                                                                                                 >  1:841482/1‑150 (MQ=255)
                                     ggTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGGTTTAGAAACTCAGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCAATACTTGGAGTAAATCACGACGATCAGGTCTTCGTTAATGTACg                                                                                                                >  2:1285635/1‑150 (MQ=255)
                                       tCACCTTGGCGTGCGTCGAACTCACTTTCTCGATATCAACAAGGCGCGGTTTTAGAAACTCTGTCACAGAAGCCTGCATTGTCTCCTCTCTTTGGGACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGca                                                                                                              >  2:929226/1‑150 (MQ=255)
                                        cACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGcag                                                                                                             >  1:1185776/1‑150 (MQ=255)
                                           cTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGcagaca                                                                                                          <  1:523789/150‑1 (MQ=255)
                                             tGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGcagacaga                                                                                                        <  2:1855963/150‑1 (MQ=255)
                                             tGGCGTGCGTCGAACTCAATTGCTCGATATAAACCAGGAGCGGTTTTAGAAACTCTGTCACAGAACCATGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTAGAAGATAATGTGTTAGTTAATGTCCGcagacaga                                                                                                        >  1:1554384/1‑150 (MQ=255)
                                              ggCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGAt                                                                                                       >  2:1344812/1‑150 (MQ=255)
                                                cgtgcgtCGAACTCACTTTCTCGATATCAACCAGTCGCTGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTGTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCa                                                                                                     <  1:267102/150‑1 (MQ=255)
                                                   gcgtCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCATGCATTGTGTCCTCTCTTTGGTACTAAGCTTTAGTTGGAGTAAAGCTCGACGATCAGGGGTTCGTTAATGTCCGCAGCCAGATCAGaa                                                                                                  >  2:1593504/1‑150 (MQ=255)
                                                    cgtCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAAc                                                                                                 >  2:1448571/1‑150 (MQ=255)
                                                        gAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTc                                                                                             <  2:332706/150‑1 (MQ=255)
                                                           cTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCgg                                                                                          >  1:539456/1‑150 (MQ=255)
                                                             cACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCt                                                                                        >  1:1733677/1‑150 (MQ=255)
                                                             cACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCt                                                                                        >  1:845662/1‑150 (MQ=255)
                                                              aCTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCTTTAATGTCCGCAGACAGATCAGAACGCTCCGGCtt                                                                                       >  1:883811/1‑150 (MQ=255)
                                                               cTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTa                                                                                      <  2:1255616/150‑1 (MQ=255)
                                                                 tGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACg                                                                                    >  2:112539/1‑150 (MQ=255)
                                                                  gCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGc                                                                                   <  2:901845/150‑1 (MQ=255)
                                                                   cTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGCCAGATCAGAACGCTCAGGCTTACGCt                                                                                  >  1:1703184/1‑150 (MQ=255)
                                                                     cGATATCAACCAGGCGCGTTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTa                                                                                <  1:1214996/150‑1 (MQ=255)
                                                                       atatCAACCAGGCGCTGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTaaa                                                                              <  2:99125/150‑1 (MQ=255)
                                                                       atatCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCAATGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCTACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTaaa                                                                              <  1:900093/150‑1 (MQ=255)
                                                                        tatCAACCAGGCGCGGTTTTAGACACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAAc                                                                             <  1:138035/150‑1 (MQ=255)
                                                                         atCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACg                                                                            <  1:664909/150‑1 (MQ=255)
                                                                             aCCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTAcc                                                                        >  1:989588/1‑150 (MQ=255)
                                                                               cAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCtt                                                                      >  2:363174/1‑150 (MQ=255)
                                                                                aGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTc                                                                     <  2:31627/150‑1 (MQ=255)
                                                                                   cgcgGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCAt                                                                  >  1:967583/1‑150 (MQ=255)
                                                                                    gcgGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATc                                                                 <  2:1038576/150‑1 (MQ=255)
                                                                                    gcgGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAACCTTTACTTGGTGTAAAGCTCGACGTTCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATc                                                                 <  1:245875/150‑1 (MQ=255)
                                                                                             gAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCa                                                        <  2:977923/150‑1 (MQ=255)
                                                                                             gAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCa                                                        >  1:639969/1‑150 (MQ=255)
                                                                                              aaaCTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAATGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCAt                                                       <  1:1845208/150‑1 (MQ=255)
                                                                                                   ctGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACt                                                  >  1:1655443/1‑150 (MQ=255)
                                                                                                   ctGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACt                                                  >  2:129380/1‑150 (MQ=255)
                                                                                                       cacaGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTcca                                              >  1:1819722/1‑150 (MQ=255)
                                                                                                       cacaGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGAACATTCCATATTTCCAGCATCAACTTcca                                              >  2:174669/1‑150 (MQ=255)
                                                                                                         caGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTccagc                                            >  2:870649/1‑150 (MQ=255)
                                                                                                           gAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTccagcca                                          <  2:358175/150‑1 (MQ=255)
                                                                                                              cccTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGt                                       >  1:1102033/1‑150 (MQ=255)
                                                                                                                  gCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGc                                   <  1:433002/150‑1 (MQ=255)
                                                                                                                  gCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGc                                   <  1:1513866/150‑1 (MQ=255)
                                                                                                                  gCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGc                                   <  1:211867/150‑1 (MQ=255)
                                                                                                                     ttGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCttt                                <  1:112539/150‑1 (MQ=255)
                                                                                                                       gtgtCCTCTCTTTGGTACTAAGCTTTACTTTGAGTAAAGCTCGACGATCAGGTGTTCGTTACTGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTc                              <  2:1380181/150‑1 (MQ=255)
                                                                                                                         gtCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCAc                            >  1:1772161/1‑150 (MQ=255)
                                                                                                                           ccTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACgc                          <  2:686409/150‑1 (MQ=255)
                                                                                                                            ctctctTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACgct                         >  2:1416617/1‑150 (MQ=255)
                                                                                                                                ctTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGAAAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGATTTTCACGCTGCTc                     >  1:1815156/1‑150 (MQ=255)
                                                                                                                                  ttGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGTTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTcag                   <  2:938964/150‑1 (MQ=255)
                                                                                                                                  ttGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTcag                   >  1:1131186/1‑150 (MQ=255)
                                                                                                                                    ggTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTcagcc                 >  2:170914/1‑150 (MQ=255)
                                                                                                                                    ggTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGATCCGGCTTACGCTAAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGCTTGGCTTTTCACGCTGCTcagcc                 >  1:1731894/1‑150 (MQ=255)
                                                                                                                                      cgtcgaGCTTTACTCCAAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTCACGCTGCTcagccagc              >  2:2774/6‑150 (MQ=255)
                                                                                                                                       gtcgaGCTTTACTCCAAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCACCTTCCAGCCAGGTTGGCTTTTCACGCTGCTcagccagc              >  1:739683/5‑150 (MQ=255)
                                                                                                                                       aCTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTcagccagc              >  2:510691/1‑150 (MQ=255)
                                                                                                                                         tAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTc            >  1:796562/1‑150 (MQ=255)
                                                                                                                                          aaGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTcc           >  1:863244/1‑150 (MQ=255)
                                                                                                                                          aaGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGGTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTcc           <  1:1446499/150‑1 (MQ=255)
                                                                                                                                           aGCTTTACTCCAAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCa          >  1:1000964/1‑150 (MQ=255)
                                                                                                                                            gCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCag         <  2:1009122/150‑1 (MQ=255)
                                                                                                                                             cTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCGTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCaga        >  1:345982/1‑150 (MQ=255)
                                                                                                                                              tttACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCagag       <  2:1473365/150‑1 (MQ=255)
                                                                                                                                              tttACTTGCAGTAAAGCTCGACGATCAGGCGATCGGTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTCGGCTTTTCACGCTGCTCAGCCAGCTCCagag       <  2:922141/150‑1 (MQ=255)
                                                                                                                                                tACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCg     <  2:1719061/150‑1 (MQ=255)
                                                                                                                                                  cTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGc   <  2:868769/150‑1 (MQ=255)
                                                                                                                                                  cTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGc   >  2:63239/1‑150 (MQ=255)
                                                                                                                                                   ttGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGCt  <  1:507411/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGCT  >  NC_000913/3440912‑3441208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: