Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,442,572 A→G 6.7% intergenic (‑101/+46) rpsM ← / ← rpmJ 30S ribosomal subunit protein S13/50S ribosomal subunit protein L36

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,442,5720AG 6.7% 349.9 / 12.2 119intergenic (‑101/+46)rpsM/rpmJ30S ribosomal subunit protein S13/50S ribosomal subunit protein L36
Reads supporting (aligned to +/- strand):  ref base A (50/60);  new base G (3/5);  total (54/65)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01

Only 100 of 120 total aligned reads displayed.

TTACGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGA  >  NC_000913/3442423‑3442719
                                                                                                                                                     |                                                                                                                                                   
ttACGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTaa                                                                                                                                                     <  1:1814576/150‑1 (MQ=255)
  aCGGAATGCTGATGCTCAGGAATGTTAATGCCTGCTATACGCGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAACTGGAAACGCACAGACATACAAAAGCTTGGCTGGCTAATc                                                                                                                                                   <  2:279993/150‑1 (MQ=255)
   cGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCt                                                                                                                                                  >  1:485091/1‑150 (MQ=255)
   cGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGAGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATca                                                                                                                                                  <  1:1117231/150‑2 (MQ=255)
     gCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAg                                                                                                                                                <  1:1312416/150‑1 (MQ=255)
      cATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGc                                                                                                                                               <  1:687800/150‑1 (MQ=255)
       aTGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGcc                                                                                                                                              <  2:1086818/150‑1 (MQ=255)
       aTGCTTATGATCAGGAATGTTAATGCCTGCTATACGCGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAATCGCACAGACATACAAAAGAATGGCTGGCTAATCTAGcc                                                                                                                                              <  2:1005348/150‑1 (MQ=255)
       aTGCTTATGATCAGGAATGTTAATCCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAATCACGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGcc                                                                                                                                              <  2:580618/150‑1 (MQ=255)
       aTGATTATGATCAGGAATGTTCATGCCTGCTATACGGGCCACTATGGACTCCTACTATTTAATATGTACGTTCCATGCTGACAAGCCCGTTTGCAGGCTACTCAACTGGAAACGCACAGCCATACAAAAGCTTGGCTGGCTAATCTAGcc                                                                                                                                              <  2:117395/150‑1 (MQ=255)
        tgattATGATCAGGAAAGTTAATGCCTGCTATAAGTTCCACTATTCACTCCTACTATTTAATATTTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACTGACATACAAAAGATTGGCTGGCTAATCTAGCCa                                                                                                                                             <  1:107109/147‑1 (MQ=255)
        tGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCa                                                                                                                                             <  2:1537925/150‑1 (MQ=255)
         gCTTATGATCAGGAATGATAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAg                                                                                                                                            <  2:1380057/150‑1 (MQ=255)
          cTTATGATCAGGAATGTTAATGCCTGCTATACGTGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAGATTAGCCAGc                                                                                                                                           <  1:200728/150‑1 (MQ=255)
          cTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAATAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGTCTGGCTAATCTAGCCAGc                                                                                                                                           >  1:652008/1‑150 (MQ=255)
           ttATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAGATTAGCCAGcc                                                                                                                                          <  1:617884/150‑2 (MQ=255)
              tGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCaa                                                                                                                                       >  2:1580907/1‑150 (MQ=255)
               gATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTTGCTAATCTATCCAGCTCAAc                                                                                                                                      >  1:267700/1‑150 (MQ=255)
               gATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAAc                                                                                                                                      >  2:585044/1‑150 (MQ=255)
               gATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAAc                                                                                                                                      <  2:542040/150‑1 (MQ=255)
               gATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAAc                                                                                                                                      <  2:143017/150‑1 (MQ=255)
                aTCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAAcc                                                                                                                                     >  2:722619/1‑150 (MQ=255)
                aTCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAAcc                                                                                                                                     >  1:258112/1‑150 (MQ=255)
                  cAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTCGCCAGCTCACCCCa                                                                                                                                   >  1:934883/1‑150 (MQ=255)
                        tGTTACTGCCTGCTATACGGGCCACTATGCACTCCTACTCTTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGACACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTg                                                                                                                             <  1:155387/150‑1 (MQ=255)
                        tGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTg                                                                                                                             <  2:1346181/150‑1 (MQ=255)
                         gTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGc                                                                                                                            >  2:1831833/1‑150 (MQ=255)
                          ttAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCa                                                                                                                           <  2:1116546/150‑1 (MQ=255)
                                 cTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAACGCCCGTTTTCAGGCTACTCAAATGGAAACGCCCAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGtaaaa                                                                                                                    >  1:934852/1‑150 (MQ=255)
                                     tataCGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATg                                                                                                                >  1:591814/1‑150 (MQ=255)
                                      ataCGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGc                                                                                                               >  1:1242411/1‑150 (MQ=255)
                                      ataCGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGc                                                                                                               >  2:703733/1‑150 (MQ=255)
                                       taCGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCg                                                                                                              <  1:462461/150‑1 (MQ=255)
                                         cGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACGTTGCAAGAAAAATATGCGaa                                                                                                            >  1:939042/1‑150 (MQ=255)
                                            gCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGaaaaa                                                                                                         <  1:1557076/150‑1 (MQ=255)
                                               aCTATGCACACCTACTATTTTATATGTACGTTCCTTGCTGAAAAGCCCGTTTTGAGGATACTCAAATGGAAACGCACAGACATACTAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATc                                                                                                      <  1:1005457/150‑1 (MQ=255)
                                                      aCTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTg                                                                                               <  2:1594005/150‑1 (MQ=255)
                                                        tCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGgc                                                                                             <  2:1448194/150‑1 (MQ=255)
                                                           tactaTTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTACTCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCt                                                                                          >  1:1112333/1‑150 (MQ=255)
                                                             ctaTTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGt                                                                                        >  1:1220825/1‑150 (MQ=255)
                                                              taTTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGtt                                                                                       <  2:1110171/150‑1 (MQ=255)
                                                              taTTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGtt                                                                                       <  1:585044/150‑1 (MQ=255)
                                                              taTTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAATATGCGAAAAAATCAGCCTTGGCGCTGttt                                                                                      <  1:1871324/150‑1 (MQ=255)
                                                              taTTTAATATGTACGTTCCATGCTGAAAAGCAAGTTTTCAGGATACTCAAATGGAAACGCAGAGACATACAAAAGATTGTCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGtt                                                                                       <  1:212255/150‑1 (MQ=255)
                                                                 ttAATATGTACGTTCCATGCTGAAAATCCCGTTTTAAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTAt                                                                                    <  1:1456431/150‑1 (MQ=255)
                                                                       tGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTtcg                                                                              >  2:709413/1‑150 (MQ=255)
                                                                       tGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTtcg                                                                              >  1:1800959/1‑150 (MQ=255)
                                                                       tGTAAGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAACTGGAAACGCACAGACATACAAACGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGAGAAAAAATCAGCCTTGGCGCTGTTTATGCTtcg                                                                              <  2:1732375/150‑1 (MQ=255)
                                                                        gTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTtcgg                                                                             >  2:426569/1‑150 (MQ=255)
                                                                          aCGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTtcggct                                                                           <  2:1736969/150‑1 (MQ=255)
                                                                                cATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCAc                                                                     >  2:548648/1‑150 (MQ=255)
                                                                                   gCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGc                                                                  >  2:42016/1‑150 (MQ=255)
                                                                                     tGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCCACCCAACTTTGAAAGAAAAATATTCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTAGGCTCGGCACTGCaa                                                                >  2:1215091/1‑150 (MQ=255)
                                                                                       aaaaGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAAt                                                              <  1:722619/150‑1 (MQ=255)
                                                                                       aaaaGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAAt                                                              <  1:1233798/150‑1 (MQ=255)
                                                                                        aaaGCCCGTTTTCAGGATACTCAAATGGAAACGCACATACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTATGCAAGAAAAATATGCGAAAAAATCTGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATc                                                             <  2:1322684/150‑1 (MQ=255)
                                                                                          aGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATcac                                                           >  1:543155/1‑150 (MQ=255)
                                                                                              cGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACgg                                                       <  2:1813239/150‑1 (MQ=255)
                                                                                                   tCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGac                                                  >  1:933222/1‑150 (MQ=255)
                                                                                                   tCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGac                                                  <  1:1527209/150‑1 (MQ=255)
                                                                                                    cAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGaca                                                 >  2:1810192/1‑150 (MQ=255)
                                                                                                       gATACTCATATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCa                                              <  2:865118/150‑1 (MQ=255)
                                                                                                         tACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATc                                            <  2:542674/150‑1 (MQ=255)
                                                                                                         tACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATc                                            >  2:1562177/1‑150 (MQ=255)
                                                                                                           cTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCAc                                          >  1:974638/1‑150 (MQ=255)
                                                                                                           cTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCAc                                          >  1:1444977/1‑150 (MQ=255)
                                                                                                            tCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACg                                         <  2:1498420/150‑1 (MQ=255)
                                                                                                             cAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGc                                        >  2:1265584/1‑150 (MQ=255)
                                                                                                                aTGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTa                                     >  1:883548/1‑150 (MQ=255)
                                                                                                                 tGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTaa                                    >  2:154224/1‑150 (MQ=255)
                                                                                                                 tGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTaa                                    <  1:662949/150‑1 (MQ=255)
                                                                                                                 tGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACATTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTaa                                    <  1:1722695/150‑1 (MQ=255)
                                                                                                                  ggAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAAc                                   <  1:376630/150‑1 (MQ=255)
                                                                                                                   gAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAGAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTGAACg                                  >  2:1521597/1‑150 (MQ=255)
                                                                                                                   gAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACg                                  >  2:1677321/1‑150 (MQ=255)
                                                                                                                   gAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACg                                  >  2:491823/1‑150 (MQ=255)
                                                                                                                   gAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACg                                  >  2:759319/1‑150 (MQ=255)
                                                                                                                     aaCGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGAt                                >  2:1781206/1‑150 (MQ=255)
                                                                                                                       cGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCCACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCCCACGGATGACCCCATCACGCTTAACGAttt                              >  2:897437/1‑150 (MQ=255)
                                                                                                                       cGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGAttt                              <  2:1242411/150‑1 (MQ=255)
                                                                                                                         cacaGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTACCGATTTTg                            >  1:800318/1‑150 (MQ=255)
                                                                                                                                  aCAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACgg                   <  2:883548/150‑1 (MQ=255)
                                                                                                                                    aaaaGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGNTTTTGCAGTTACGGCa                 <  1:585284/150‑1 (MQ=255)
                                                                                                                                         aTTGGCTGGCTAATCTAGCCAGCTCAACCAAACTTTGCAAGAAAAATATGCGAAAAAATCCGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGCCACCATCCCGCTTAACGATTTTGCAGTTTCGGCATAAtt            >  2:202594/1‑150 (MQ=255)
                                                                                                                                          tgagctggctAGAGTAGCCAGCTCCACCCAACGTTGCCAGTAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCGTCGGCTCGCCACTGCAAATCACACCGATGCCGCGATCAGGCTTATCGATTTTGGAGTTTAGGCATAAttt           >  2:102841/4‑150 (MQ=255)
                                                                                                                                            ggctggctAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCt         >  2:111214/1‑150 (MQ=255)
                                                                                                                                            agctggctAGATTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTTACGATTTTGCAGTTACGGAATAATTTCt         >  2:1284560/2‑150 (MQ=255)
                                                                                                                                            agctggctAGATTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCt         <  2:64785/149‑1 (MQ=255)
                                                                                                                                             gctggctAATCTAGCCAGCTCAACCCCACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCCTCACGCTTAACGATTTTGCAGTTACGGCATAATTTCtt        >  1:1153576/1‑150 (MQ=255)
                                                                                                                                             gctggctAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCtt        <  2:375896/150‑1 (MQ=255)
                                                                                                                                             gctggctAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCtt        >  1:1699841/1‑150 (MQ=255)
                                                                                                                                              ctggctAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCGTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTg       >  1:1250049/1‑150 (MQ=255)
                                                                                                                                               tggctAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGa      <  1:614769/150‑1 (MQ=255)
                                                                                                                                                ggctAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGAttt                              >  2:1664142/1‑125 (MQ=255)
                                                                                                                                                ggctAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGAttt                              <  1:1664142/125‑1 (MQ=255)
                                                                                                                                                 gctAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACg    <  1:407712/150‑1 (MQ=255)
                                                                                                                                                  ctAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCAGCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACgg   >  2:34694/1‑150 (MQ=255)
                                                                                                                                                   tAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCATCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGa  <  1:959824/150‑1 (MQ=255)
                                                                                                                                                   tAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGa  <  2:943326/150‑1 (MQ=255)
                                                                                                                                                   tAATCTAGCCAGCTCAACCCAACGTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGa  <  2:939042/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTACGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGA  >  NC_000913/3442423‑3442719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: