Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,442,574 C→T 6.2% intergenic (‑103/+44) rpsM ← / ← rpmJ 30S ribosomal subunit protein S13/50S ribosomal subunit protein L36

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,442,5740CT 6.2% 374.7 / 7.6 118intergenic (‑103/+44)rpsM/rpmJ30S ribosomal subunit protein S13/50S ribosomal subunit protein L36
Reads supporting (aligned to +/- strand):  ref base C (52/58);  new base T (2/5);  total (55/63)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

ACGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGC  >  NC_000913/3442425‑3442722
                                                                                                                                                     |                                                                                                                                                    
aCGGAATGCTGATGCTCAGGAATGTTAATGCCTGCTATACGCGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAACTGGAAACGCACAGACATACAAAAGCTTGGCTGGCTAATc                                                                                                                                                      <  2:279993/150‑1 (MQ=255)
 cGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCt                                                                                                                                                     >  1:485091/1‑150 (MQ=255)
 cGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGAGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATca                                                                                                                                                     <  1:1117231/150‑2 (MQ=255)
   gCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAg                                                                                                                                                   <  1:1312416/150‑1 (MQ=255)
    cATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGc                                                                                                                                                  <  1:687800/150‑1 (MQ=255)
     aTGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGcc                                                                                                                                                 <  2:1086818/150‑1 (MQ=255)
     aTGCTTATGATCAGGAATGTTAATGCCTGCTATACGCGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAATCGCACAGACATACAAAAGAATGGCTGGCTAATCTAGcc                                                                                                                                                 <  2:1005348/150‑1 (MQ=255)
     aTGCTTATGATCAGGAATGTTAATCCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAATCACGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGcc                                                                                                                                                 <  2:580618/150‑1 (MQ=255)
     aTGATTATGATCAGGAATGTTCATGCCTGCTATACGGGCCACTATGGACTCCTACTATTTAATATGTACGTTCCATGCTGACAAGCCCGTTTGCAGGCTACTCAACTGGAAACGCACAGCCATACAAAAGCTTGGCTGGCTAATCTAGcc                                                                                                                                                 <  2:117395/150‑1 (MQ=255)
      tgattATGATCAGGAAAGTTAATGCCTGCTATAAGTTCCACTATTCACTCCTACTATTTAATATTTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACTGACATACAAAAGATTGGCTGGCTAATCTAGCCa                                                                                                                                                <  1:107109/147‑1 (MQ=255)
      tGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCa                                                                                                                                                <  2:1537925/150‑1 (MQ=255)
       gCTTATGATCAGGAATGATAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAg                                                                                                                                               <  2:1380057/150‑1 (MQ=255)
        cTTATGATCAGGAATGTTAATGCCTGCTATACGTGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAGATTAGCCAGc                                                                                                                                              <  1:200728/150‑1 (MQ=255)
        cTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAATAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGTCTGGCTAATCTAGCCAGc                                                                                                                                              >  1:652008/1‑150 (MQ=255)
         ttATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAGATTAGCCAGcc                                                                                                                                             <  1:617884/150‑2 (MQ=255)
            tGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCaa                                                                                                                                          >  2:1580907/1‑150 (MQ=255)
             gATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTTGCTAATCTATCCAGCTCAAc                                                                                                                                         >  1:267700/1‑150 (MQ=255)
             gATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAAc                                                                                                                                         <  2:143017/150‑1 (MQ=255)
             gATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAAc                                                                                                                                         <  2:542040/150‑1 (MQ=255)
             gATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAAc                                                                                                                                         >  2:585044/1‑150 (MQ=255)
              aTCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAAcc                                                                                                                                        >  2:722619/1‑150 (MQ=255)
              aTCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAAcc                                                                                                                                        >  1:258112/1‑150 (MQ=255)
                cAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTCGCCAGCTCACCCCa                                                                                                                                      >  1:934883/1‑150 (MQ=255)
                      tGTTACTGCCTGCTATACGGGCCACTATGCACTCCTACTCTTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGACACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTg                                                                                                                                <  1:155387/150‑1 (MQ=255)
                      tGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTg                                                                                                                                <  2:1346181/150‑1 (MQ=255)
                       gTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGc                                                                                                                               >  2:1831833/1‑150 (MQ=255)
                        ttAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCa                                                                                                                              <  2:1116546/150‑1 (MQ=255)
                               cTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAACGCCCGTTTTCAGGCTACTCAAATGGAAACGCCCAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGtaaaa                                                                                                                       >  1:934852/1‑150 (MQ=255)
                                   tataCGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATg                                                                                                                   >  1:591814/1‑150 (MQ=255)
                                    ataCGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGc                                                                                                                  >  1:1242411/1‑150 (MQ=255)
                                    ataCGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGc                                                                                                                  >  2:703733/1‑150 (MQ=255)
                                     taCGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCg                                                                                                                 <  1:462461/150‑1 (MQ=255)
                                       cGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACGTTGCAAGAAAAATATGCGaa                                                                                                               >  1:939042/1‑150 (MQ=255)
                                          gCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGaaaaa                                                                                                            <  1:1557076/150‑1 (MQ=255)
                                             aCTATGCACACCTACTATTTTATATGTACGTTCCTTGCTGAAAAGCCCGTTTTGAGGATACTCAAATGGAAACGCACAGACATACTAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATc                                                                                                         <  1:1005457/150‑1 (MQ=255)
                                                    aCTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTg                                                                                                  <  2:1594005/150‑1 (MQ=255)
                                                      tCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGgc                                                                                                <  2:1448194/150‑1 (MQ=255)
                                                         tactaTTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTACTCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCt                                                                                             >  1:1112333/1‑150 (MQ=255)
                                                           ctaTTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGt                                                                                           >  1:1220825/1‑150 (MQ=255)
                                                            taTTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGtt                                                                                          <  2:1110171/150‑1 (MQ=255)
                                                            taTTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGtt                                                                                          <  1:585044/150‑1 (MQ=255)
                                                            taTTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAATATGCGAAAAAATCAGCCTTGGCGCTGttt                                                                                         <  1:1871324/150‑1 (MQ=255)
                                                            taTTTAATATGTACGTTCCATGCTGAAAAGCAAGTTTTCAGGATACTCAAATGGAAACGCAGAGACATACAAAAGATTGTCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGtt                                                                                          <  1:212255/150‑1 (MQ=255)
                                                               ttAATATGTACGTTCCATGCTGAAAATCCCGTTTTAAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTAt                                                                                       <  1:1456431/150‑1 (MQ=255)
                                                                     tGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTtcg                                                                                 >  2:709413/1‑150 (MQ=255)
                                                                     tGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTtcg                                                                                 >  1:1800959/1‑150 (MQ=255)
                                                                     tGTAAGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAACTGGAAACGCACAGACATACAAACGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGAGAAAAAATCAGCCTTGGCGCTGTTTATGCTtcg                                                                                 <  2:1732375/150‑1 (MQ=255)
                                                                      gTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTtcgg                                                                                >  2:426569/1‑150 (MQ=255)
                                                                        aCGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTtcggct                                                                              <  2:1736969/150‑1 (MQ=255)
                                                                              cATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCAc                                                                        >  2:548648/1‑150 (MQ=255)
                                                                                 gCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGc                                                                     >  2:42016/1‑150 (MQ=255)
                                                                                   tGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCCACCCAACTTTGAAAGAAAAATATTCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTAGGCTCGGCACTGCaa                                                                   >  2:1215091/1‑150 (MQ=255)
                                                                                     aaaaGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAAt                                                                 <  1:1233798/150‑1 (MQ=255)
                                                                                     aaaaGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAAt                                                                 <  1:722619/150‑1 (MQ=255)
                                                                                      aaaGCCCGTTTTCAGGATACTCAAATGGAAACGCACATACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTATGCAAGAAAAATATGCGAAAAAATCTGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATc                                                                <  2:1322684/150‑1 (MQ=255)
                                                                                        aGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATcac                                                              >  1:543155/1‑150 (MQ=255)
                                                                                            cGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACgg                                                          <  2:1813239/150‑1 (MQ=255)
                                                                                                 tCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGac                                                     <  1:1527209/150‑1 (MQ=255)
                                                                                                 tCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGac                                                     >  1:933222/1‑150 (MQ=255)
                                                                                                  cAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGaca                                                    >  2:1810192/1‑150 (MQ=255)
                                                                                                     gATACTCATATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCa                                                 <  2:865118/150‑1 (MQ=255)
                                                                                                       tACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATc                                               >  2:1562177/1‑150 (MQ=255)
                                                                                                       tACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATc                                               <  2:542674/150‑1 (MQ=255)
                                                                                                         cTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCAc                                             >  1:1444977/1‑150 (MQ=255)
                                                                                                         cTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCAc                                             >  1:974638/1‑150 (MQ=255)
                                                                                                          tCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACg                                            <  2:1498420/150‑1 (MQ=255)
                                                                                                           cAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGc                                           >  2:1265584/1‑150 (MQ=255)
                                                                                                              aTGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTa                                        >  1:883548/1‑150 (MQ=255)
                                                                                                               tGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTaa                                       <  1:662949/150‑1 (MQ=255)
                                                                                                               tGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTaa                                       >  2:154224/1‑150 (MQ=255)
                                                                                                               tGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACATTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTaa                                       <  1:1722695/150‑1 (MQ=255)
                                                                                                                ggAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAAc                                      <  1:376630/150‑1 (MQ=255)
                                                                                                                 gAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAGAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTGAACg                                     >  2:1521597/1‑150 (MQ=255)
                                                                                                                 gAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACg                                     >  2:1677321/1‑150 (MQ=255)
                                                                                                                 gAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACg                                     >  2:491823/1‑150 (MQ=255)
                                                                                                                 gAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACg                                     >  2:759319/1‑150 (MQ=255)
                                                                                                                   aaCGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGAt                                   >  2:1781206/1‑150 (MQ=255)
                                                                                                                     cGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCCACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCCCACGGATGACCCCATCACGCTTAACGAttt                                 >  2:897437/1‑150 (MQ=255)
                                                                                                                     cGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGAttt                                 <  2:1242411/150‑1 (MQ=255)
                                                                                                                       cacaGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTACCGATTTTg                               >  1:800318/1‑150 (MQ=255)
                                                                                                                                aCAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACgg                      <  2:883548/150‑1 (MQ=255)
                                                                                                                                  aaaaGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGNTTTTGCAGTTACGGCa                    <  1:585284/150‑1 (MQ=255)
                                                                                                                                       aTTGGCTGGCTAATCTAGCCAGCTCAACCAAACTTTGCAAGAAAAATATGCGAAAAAATCCGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGCCACCATCCCGCTTAACGATTTTGCAGTTTCGGCATAAtt               >  2:202594/1‑150 (MQ=255)
                                                                                                                                        tgagctggctAGAGTAGCCAGCTCCACCCAACGTTGCCAGTAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCGTCGGCTCGCCACTGCAAATCACACCGATGCCGCGATCAGGCTTATCGATTTTGGAGTTTAGGCATAAttt              >  2:102841/4‑150 (MQ=255)
                                                                                                                                          ggctggctAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCt            >  2:111214/1‑150 (MQ=255)
                                                                                                                                          agctggctAGATTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTTACGATTTTGCAGTTACGGAATAATTTCt            >  2:1284560/2‑150 (MQ=255)
                                                                                                                                          agctggctAGATTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCt            <  2:64785/149‑1 (MQ=255)
                                                                                                                                           gctggctAATCTAGCCAGCTCAACCCCACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCCTCACGCTTAACGATTTTGCAGTTACGGCATAATTTCtt           >  1:1153576/1‑150 (MQ=255)
                                                                                                                                           gctggctAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCtt           <  2:375896/150‑1 (MQ=255)
                                                                                                                                           gctggctAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCtt           >  1:1699841/1‑150 (MQ=255)
                                                                                                                                            ctggctAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCGTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTg          >  1:1250049/1‑150 (MQ=255)
                                                                                                                                             tggctAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGa         <  1:614769/150‑1 (MQ=255)
                                                                                                                                              ggctAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGAttt                                 >  2:1664142/1‑125 (MQ=255)
                                                                                                                                              ggctAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGAttt                                 <  1:1664142/125‑1 (MQ=255)
                                                                                                                                               gctAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACg       <  1:407712/150‑1 (MQ=255)
                                                                                                                                                ctAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCAGCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACgg      >  2:34694/1‑150 (MQ=255)
                                                                                                                                                 tAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCATCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGa     <  1:959824/150‑1 (MQ=255)
                                                                                                                                                 tAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGa     <  2:943326/150‑1 (MQ=255)
                                                                                                                                                 tAATCTAGCCAGCTCAACCCAACGTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGa     <  2:939042/150‑1 (MQ=255)
                                                                                                                                                    tCTAGCCAGCTCACCCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGc  >  1:1811368/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGC  >  NC_000913/3442425‑3442722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: