Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,458,110 C→A 100% T578T (ACC→ACA gspD → general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,458,1100CA100.0% 365.2 / ‑6.7 113T578T (ACC→ACAgspDgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base C (0/2);  major base A (61/48);  minor base T (1/1);  total (62/51)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 115 total aligned reads displayed.

ACTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACCGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGT  >  NC_000913/3457964‑3458256
                                                                                                                                                  |                                                                                                                                                   
 cTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCACCTCTTCCGCTATACCTCCACAGAgc                                                                                                                                                 >  2:1640077/1‑150 (MQ=255)
 cTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgc                                                                                                                                                 >  1:1693699/1‑150 (MQ=255)
   aTTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgcgc                                                                                                                                               >  1:1562412/1‑150 (MQ=255)
    ttCAAAACGCCGTGCTGGTCAAAACCGGTGAACCGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAATGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgcgcg                                                                                                                                              <  1:1246973/150‑1 (MQ=255)
    ttCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgcgcg                                                                                                                                              >  1:1836102/1‑150 (MQ=255)
     tCAAAACGCCGTGCTGGTCAAAACCGGTGACACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgcgcgc                                                                                                                                             >  1:1653921/1‑150 (MQ=255)
     tCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCGTGGGCGGATTGGTGGTTGATTTTTATAAAGAGCAAGTGTCAAAGGTTCCTAGGATTGGCTATTTGCATTTTTTGGGGCAAAGATTCCGCTTTCCTTCAACAGGgcgcgc                                                                                                                                             >  2:1163305/1‑150 (MQ=255)
     tCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgcgcgc                                                                                                                                             >  2:1479698/1‑150 (MQ=255)
      cAAAACGCCGTGCTGGTCAAAACCGGTGAACCGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCt                                                                                                                                            <  2:1136678/150‑1 (MQ=255)
      cAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCt                                                                                                                                            >  2:1613910/1‑150 (MQ=255)
      cAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCt                                                                                                                                            <  1:1464770/150‑1 (MQ=255)
       aaaaCGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTa                                                                                                                                           >  2:12528/1‑150 (MQ=255)
          aCGCCGTGCTGGTCAACACCGGTGAAACGGTGGTCCTGGGCGGATTGCGGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAAc                                                                                                                                        <  1:989546/150‑1 (MQ=255)
          aCGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTTGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAAc                                                                                                                                        <  2:1781816/150‑1 (MQ=255)
           cGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACg                                                                                                                                       <  1:1467560/150‑1 (MQ=255)
               tttcTGCTCAAAACCGGTGAAACGGTGGTCCAGGGCGGATTGCTTGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTCGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAAc                                                                                                                                   <  2:159667/147‑1 (MQ=255)
               gTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGAAAGTGTCAAAGGTTCCTCTGCTTGTCGATATTCGTTTAGTGGGGCAACTCTGCCGCGATCCCTCCACAGAGCGCGCTAAACCCAAc                                                                                                                                   >  2:339820/1‑150 (MQ=255)
                   tGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGa                                                                                                                               >  2:1751085/1‑150 (MQ=255)
                   tGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGa                                                                                                                               >  1:1785943/1‑150 (MQ=255)
                    ggTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTACGAAAGAGCAAGTGTCAAAGGTTCCTCTGCTGGGCGCTATTCCTTTAGTGGGGCAACTCTACCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTgct                                                                                                                              >  1:1857638/1‑148 (MQ=255)
                     gTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGGGCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATg                                                                                                                             <  2:1790738/150‑1 (MQ=255)
                      gcAAATCCGGTGAAACTGTGGTCCTGGGCGGAGTGCTGGATGATTTTTCTAAAGATCAAGTGTCAAATTTGCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACATAGCGCGCTAAACGCTACCTGATgg                                                                                                                            <  2:463834/149‑1 (MQ=255)
                       cAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGt                                                                                                                           >  2:680523/1‑150 (MQ=255)
                        aaaaCCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTa                                                                                                                          <  2:28808/150‑1 (MQ=255)
                        aaaaCCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTa                                                                                                                          <  1:1402646/150‑1 (MQ=255)
                          aaCCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTAtt                                                                                                                        <  2:1220478/150‑1 (MQ=255)
                            ccGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTa                                                                                                                      <  2:1519518/150‑1 (MQ=255)
                              ggTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtc                                                                                                                    <  2:64637/150‑1 (MQ=255)
                                tGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtccg                                                                                                                  <  1:1151987/150‑1 (MQ=255)
                                 gAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtccgt                                                                                                                 >  1:729923/1‑150 (MQ=255)
                                 gAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTATCTAAAGACCAACAGTCAAAGGATCCACTGCTTGGCGATAATCCTGTAGGGGGGAAACTCTTCCGCTATACCTCCACTGAGCGCGCTAAACGCAACCTGATGGTAGTTCtccgt                                                                                                                 >  2:287852/1‑150 (MQ=255)
                                  aaaCGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtccgtc                                                                                                                <  1:799688/150‑1 (MQ=255)
                                       gtggtCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCcgacg                                                                                                           <  1:1846966/150‑1 (MQ=255)
                                        tggtCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCcgacga                                                                                                          <  1:1547516/150‑1 (MQ=255)
                                           tCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTa                                                                                                       >  2:624651/1‑150 (MQ=255)
                                           tCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTa                                                                                                       <  1:1350973/150‑1 (MQ=255)
                                             cTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATc                                                                                                     <  1:1660110/150‑1 (MQ=255)
                                             cTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCGATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATc                                                                                                     <  1:1405677/150‑1 (MQ=255)
                                                       tGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGGGACgatg                                                                                           <  2:244854/150‑1 (MQ=255)
                                                         cTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACgatgat                                                                                         <  2:662137/150‑1 (MQ=255)
                                                            gatgatTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGtt                                                                                      <  2:1468063/150‑1 (MQ=255)
                                                             atgatTTTTCTAACGAGCAAGTGTCAAAGGTGCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACCGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTCTCCGTGACGATGATGttt                                                                                     <  2:1405274/150‑1 (MQ=255)
                                                             atgatTTTTCTAAAGAGCCAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATCCCTCCACAGAGCGCGCTTAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGttt                                                                                     <  1:88263/150‑1 (MQ=255)
                                                             aggatGTTTCTAAAGAGCAAGTGTCAACGGTGCCTCTGCTTGGCGATATTCCTTTCGGGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGAGGGAATTTATCCGTCCGACGATTATCCGTGACGATGATGttt                                                                                     <  1:83959/148‑1 (MQ=255)
                                                                 tttttCTAAAGAGCAAGTGTCAAAGGTGCCTCTGCTTGGCGATAATCCTTTAGTCGGGCAACTCTTCCGCTAAACCTCCACAGAGCGCGCTAAACGCAACCTGAAGGTATTTCTCCGACCGACGAATAACCGTGACGATCATGTTTATCg                                                                                 >  2:1641258/1‑150 (MQ=255)
                                                                  ttttCTAAAGATGAATTTTCATAGGTTCCTATGCTTGGAGATAATCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATTGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGc                                                                                <  1:160158/150‑1 (MQ=255)
                                                                  ttttCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGc                                                                                <  1:345085/150‑1 (MQ=255)
                                                                  ttttCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATAATCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCCCAGAGCGCGGTAAACGCCACCTGAAGGTATTTCTACGTCCGAGGATTAACCGTGACGGTGATGTTTATCGc                                                                                >  2:277192/1‑150 (MQ=255)
                                                                     tCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATAGCTCa                                                                             >  2:1421469/1‑150 (MQ=255)
                                                                      cTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCAc                                                                            >  2:1643039/1‑150 (MQ=255)
                                                                       tAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACGTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACt                                                                           >  1:57486/1‑150 (MQ=255)
                                                                        aaaGAGCAAGTGTCAACGGGTCCTCTGCTGGGCGCTAATCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTg                                                                          <  2:1389050/150‑1 (MQ=255)
                                                                        aaaGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTg                                                                          <  1:582951/150‑1 (MQ=255)
                                                                          agagCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTc                                                                        >  2:1354973/1‑150 (MQ=255)
                                                                            agCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCGGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTTTCGCTCACTGTCaa                                                                      >  2:866106/1‑150 (MQ=255)
                                                                              cAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGACAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCaaaa                                                                   >  1:431875/1‑150 (MQ=255)
                                                                                aGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAaga                                                                 <  1:1754171/150‑1 (MQ=255)
                                                                                  tgtCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAgaga                                                               <  1:578432/150‑1 (MQ=255)
                                                                                  tgtCAAAGGCTCCTCTGCTTGGCGATATTCCTTTAGTGGGTCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACTCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAgaga                                                               <  2:1682023/150‑1 (MQ=255)
                                                                                   gtCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGaa                                                              >  2:795337/1‑150 (MQ=255)
                                                                                   gtCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGaa                                                              >  1:404500/1‑150 (MQ=255)
                                                                                   gtCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGaa                                                              >  2:1521348/1‑150 (MQ=255)
                                                                                    tCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGaaa                                                             >  1:567399/1‑150 (MQ=255)
                                                                                     cAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAAt                                                            <  1:1097286/150‑1 (MQ=255)
                                                                                             ccTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGAGGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATCCACCCGt                                                    >  2:1143414/1‑150 (MQ=255)
                                                                                              ctctGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGtt                                                   >  2:1525786/1‑150 (MQ=255)
                                                                                                  gCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCg                                               >  2:1612464/1‑150 (MQ=255)
                                                                                                    ttGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTc                                             <  1:1822369/150‑1 (MQ=255)
                                                                                                        cGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGa                                         >  2:1750216/1‑150 (MQ=255)
                                                                                                        cGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGa                                         >  1:646605/1‑150 (MQ=255)
                                                                                                        cGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGa                                         >  2:37617/1‑150 (MQ=255)
                                                                                                         gATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAg                                        <  2:607942/150‑1 (MQ=255)
                                                                                                         gATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAg                                        >  1:926296/1‑150 (MQ=255)
                                                                                                          atatTCCTTTAGTGTGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGc                                       <  2:1724110/150‑1 (MQ=255)
                                                                                                          atatTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGc                                       >  1:5512/1‑150 (MQ=255)
                                                                                                             atCCTTTAGTGGGGCAACTCTTCCGCTATACCTCATCAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGcaac                                    <  2:1667210/149‑1 (MQ=255)
                                                                                                              tCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGcaaca                                   >  2:508784/1‑150 (MQ=255)
                                                                                                                cTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGCGcaccaaa                                 >  2:154482/1‑149 (MQ=255)
                                                                                                                cTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCaacaac                                 >  1:1203584/1‑150 (MQ=255)
                                                                                                                cTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCaacaac                                 >  1:1245412/1‑150 (MQ=255)
                                                                                                                 tttAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAacaaca                                >  1:1642385/1‑150 (MQ=255)
                                                                                                                   tAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGc                              <  1:1572237/150‑1 (MQ=255)
                                                                                                                   tAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGc                              >  1:1519375/1‑150 (MQ=255)
                                                                                                                   tAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGc                              <  2:52023/150‑1 (MQ=255)
                                                                                                                     gTGGGGCATCTCTTCTGCTATACGTCCCCTGAGCGCGCTAAACTGAACGTGAAGGTATTTTTCCGTCCGACGATAATCCGTGACTTTGATGTTTATCGGTCACTTTCAAAATAGAAATACACCCGTTACCGTCAGGAGCTACAACAGCgg                            <  2:1002849/150‑1 (MQ=255)
                                                                                                                         ggCAACTCTTCCGCTATACCTCCACAGAGCGCGCTACACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTAACGCGCACGGTCAAAAGAGAAATACACCCGTTACCGTCCGGAGCAAGAACAGCGGATCg                        >  2:78418/1‑150 (MQ=255)
                                                                                                                         ggCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCg                        >  1:819698/1‑150 (MQ=255)
                                                                                                                         ggCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCg                        <  1:795337/150‑1 (MQ=255)
                                                                                                                         ggCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCg                        >  2:1279865/1‑150 (MQ=255)
                                                                                                                            aaCTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACg                     >  2:334533/1‑150 (MQ=255)
                                                                                                                                ctTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGaa                 >  1:1098348/1‑150 (MQ=255)
                                                                                                                                   ccGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAATAGAAATTACACCCGTAACGGACCGGACCACCAACGGCGGTTGGCCGGGaataa              >  2:494846/1‑147 (MQ=255)
                                                                                                                                    cagagagccTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCa             <  1:413993/143‑1 (MQ=255)
                                                                                                                                     gCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCaa            >  2:1491910/1‑150 (MQ=255)
                                                                                                                                        ttaCCTCCACAGAGCGCGCTAAAAGCAACCTGATGGTATTTATCCGTCCGACGATTATCCTTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAg         <  1:273894/149‑1 (MQ=255)
                                                                                                                                         taCATCCACAGAGCGCGCTAACCGAAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAgc        <  1:339999/150‑1 (MQ=255)
                                                                                                                                           ccTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAgcgc      >  1:856964/1‑150 (MQ=255)
                                                                                                                                            cTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGATACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCt     >  1:293717/1‑150 (MQ=255)
                                                                                                                                               cacaGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGt  >  2:797738/1‑150 (MQ=255)
                                                                                                                                               cACCGAGCGCGCTAAACGCAACCTTATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGt  <  1:1163305/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ACTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACCGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGT  >  NC_000913/3457964‑3458256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: