Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,471,357 C→T 8.3% intergenic (‑28/+43) tufA ← / ← fusA translation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,471,3570CT 8.3% 321.5 / 12.2 108intergenic (‑28/+43)tufA/fusAtranslation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand):  ref base C (55/44);  new base T (5/4);  total (60/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 116 total aligned reads displayed.

CGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGC  >  NC_000913/3471209‑3471505
                                                                                                                                                    |                                                                                                                                                    
cgTAGGTTTTAGCGAGTACGGTTGTGATTGCATCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTccc                                                                                                                                                     <  1:58714/150‑1 (MQ=255)
 gTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTcccc                                                                                                                                                    >  2:1569716/1‑150 (MQ=255)
     gTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCa                                                                                                                                                <  1:1616899/150‑1 (MQ=255)
           gCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                                                                                                          >  2:1820323/1‑150 (MQ=255)
           gCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                                                                                                          >  1:1530739/1‑150 (MQ=255)
           gCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                                                                                                          <  1:8554/150‑1 (MQ=255)
            ccAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagaga                                                                                                                                         >  2:1214157/1‑150 (MQ=255)
              aGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGc                                                                                                                                       >  2:1786811/1‑150 (MQ=255)
                tACGGTGGTGATTGCAGCGGGCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                                                                     <  1:1171341/150‑1 (MQ=21)
                  cGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCActa                                                                                                                                   <  1:1451439/150‑3 (MQ=255)
                  cGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACgg                                                                                                                                   <  1:843904/150‑1 (MQ=255)
                  cGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACgg                                                                                                                                   <  1:1706805/150‑1 (MQ=255)
                   ggtggtGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCATCGTGGCCTATAGAACCAACGTTAACGTGCTGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCActat                                                                                                                                  <  1:346643/150‑4 (MQ=255)
                     tggtgATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGAc                                                                                                                                >  1:904136/1‑150 (MQ=255)
                      ggtgATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGCTAGTACCAACGTTAACGTGCGGTTTTGTACTTTCAAATTTTTCTTTAGACTCGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGTCt                                                                                                                               >  1:305390/1‑150 (MQ=255)
                       gtgATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACtt                                                                                                                              >  1:1135047/1‑150 (MQ=255)
                        tgATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACttt                                                                                                                             >  1:802318/1‑150 (MQ=255)
                         gATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTg                                                                                                                            >  2:509109/1‑150 (MQ=255)
                         gATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTg                                                                                                                            >  2:1780208/1‑150 (MQ=255)
                           ttGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGt                                                                                                                          <  2:1061049/150‑1 (MQ=255)
                           ttGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGt                                                                                                                          >  1:170416/1‑150 (MQ=255)
                             gCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAt                                                                                                                        >  2:1164464/1‑150 (MQ=255)
                             gCAGCGGTCAGAGTAGATTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACAAGGCTATATTCCTTACTATAGTGCTCTCCACTTCAGGAGAGAGCCCGGGACTTAGGTAt                                                                                                                        >  1:318915/1‑150 (MQ=25)
                              cAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAtt                                                                                                                       >  1:1358225/1‑150 (MQ=255)
                                 gggTCAGTGTATTTTTACCGTGGTCATGGGGGCCGATAGTACCCACGTTAAGGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGAAACGGGTTTATTCCTTACTATAGTGCTCTCCCCTTCAGGTGAGAGCACGGGACTTTGGTATTAAc                                                                                                                    <  2:786754/149‑1 (MQ=255)
                                 cGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAc                                                                                                                    <  1:1240888/150‑1 (MQ=255)
                                    tCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCt                                                                                                                 <  2:1742060/150‑1 (MQ=255)
                                     cAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAGCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCtt                                                                                                                >  2:626982/1‑150 (MQ=255)
                                        agtagtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACTTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                                                                                                             <  2:719841/150‑1 (MQ=255)
                                        agtagtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGGGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                                                                                                             <  2:336466/150‑1 (MQ=255)
                                         ttatttttCCCGTTGTCCACGTGGCCTATTTTACAAACGTTAAAGTGCTGTTTTGTCAGTTCAAATTTTTATTTTTACACGGCTATATTCTTTACTTTAGTGCTCTCCCCTTCAGGTGAGAGCACGGGACTTTGGTATTAACCCTTAGGc                                                                                                            <  1:699210/146‑1 (MQ=255)
                                         gtagtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGc                                                                                                            >  1:1081694/1‑150 (MQ=255)
                                            gtTTTACCGTGGTCAACGTGGCCGATAGTCCAAACGTTCACGTGCGGTTTTGTACGTTCTAAGTTTTCTTTAGACCCGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGCGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                                                                         >  2:886496/1‑150 (MQ=37)
                                            gtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCTAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                                                                         >  2:1211052/1‑150 (MQ=255)
                                            gtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                                                                         >  2:1260761/1‑150 (MQ=255)
                                            gtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCATTAGAAACGGCTATATACCTTACTATAGTTCTCTCCCCTTCAGGAGAGAGAACGGGACTTTGGTATTAACCCTTATGCTTa                                                                                                         >  1:1535387/1‑150 (MQ=37)
                                              tttACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAtt                                                                                                       <  1:105998/150‑1 (MQ=255)
                                                 aCCGTGGGCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                                                                    <  1:1250225/150‑1 (MQ=255)
                                                  ccGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAcc                                                                                                   >  1:1592641/1‑150 (MQ=255)
                                                    gTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCAc                                                                                                 <  1:261475/150‑1 (MQ=255)
                                                      ggTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTACTTACTATAGTGCTCTCCCCTGCAGGAGAGAGCACGGGACTTTAGTATTAACCCTTAGGCTTATTTACCACgg                                                                                               >  1:1067846/1‑150 (MQ=255)
                                                        tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACgggg                                                                                             >  2:1233326/1‑149 (MQ=255)
                                                         aaaggTGGCCTATAGACCCAAGTTAAAGGTGCGGTTTTGAACTTTAAAATTTTTTTTTTTCAACGGTATTTTTCCTTATTATTGTGCTCCCCCATTCCGGAGATAGAACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCt                                                                                            <  2:1434758/146‑1 (MQ=255)
                                                          aaCGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGCACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCtt                                                                                           >  2:1770907/1‑150 (MQ=255)
                                                             gTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                                                                        >  1:150455/1‑150 (MQ=255)
                                                                gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTa                                                                                     >  1:1227863/1‑150 (MQ=255)
                                                                gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTa                                                                                     >  1:1862482/1‑150 (MQ=255)
                                                                  cGATAGTACCAACGTTAACGTGCGGTTTTGTACCGTCAAATATTGCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACg                                                                                   <  2:1131574/150‑1 (MQ=255)
                                                                   gATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACgg                                                                                  <  2:1600171/150‑1 (MQ=255)
                                                                      aGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCt                                                                               <  2:515365/150‑1 (MQ=255)
                                                                        tACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTgg                                                                             >  2:1011255/1‑149 (MQ=255)
                                                                         aCCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                                                                            >  1:413638/1‑150 (MQ=255)
                                                                          ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGc                                                                           >  1:193655/1‑150 (MQ=255)
                                                                           cAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCa                                                                          >  2:446786/1‑150 (MQ=255)
                                                                              cGTTATCGTGCGGGTTTGTACGTTCAAATTTTGCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCCGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACg                                                                       <  2:33026/150‑1 (MQ=255)
                                                                                ttAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGtt                                                                     <  2:1306507/150‑1 (MQ=255)
                                                                                 tAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTTCTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTa                                                                    <  2:70504/150‑1 (MQ=255)
                                                                                   aCGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGAGTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCATTTACGGCCTGAGCAACGTTACt                                                                  >  2:1276667/1‑150 (MQ=255)
                                                                                     gTGCGGTTTTGTACGTTCAAATTTTTGTTTAGACACAGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGCTAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCg                                                                <  1:515420/150‑1 (MQ=255)
                                                                                     gTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTAGTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGAATTTGGTATTAACCATTAGGCTTATTTACCACGGGCTTAATTTACGGCCTGAGCAACGTTACTCg                                                                >  1:665479/1‑150 (MQ=255)
                                                                                       gCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc                                                              >  1:624416/1‑150 (MQ=255)
                                                                                       gCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAACACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc                                                              >  1:1350120/1‑150 (MQ=255)
                                                                                        cGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACTGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg                                                             >  1:237799/1‑150 (MQ=255)
                                                                                        cGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGAGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg                                                             >  2:1093236/1‑150 (MQ=255)
                                                                                          gTTTTGTACGTTCAAATTTTTCTTTAGACTCGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt                                                           >  1:399940/1‑150 (MQ=255)
                                                                                           ttttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCtt                                                          <  2:853618/150‑1 (MQ=255)
                                                                                            tttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc                                                         >  2:1054360/1‑150 (MQ=255)
                                                                                             ttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtca                                                        <  2:1020415/150‑1 (MQ=255)
                                                                                              tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcat                                                       <  2:332268/150‑1 (MQ=255)
                                                                                               gTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc                                                      <  1:46086/150‑1 (MQ=255)
                                                                                               gTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc                                                      >  2:1514230/1‑150 (MQ=255)
                                                                                               gTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc                                                      <  2:1135047/150‑1 (MQ=255)
                                                                                                tACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatca                                                     >  2:1017811/1‑150 (MQ=255)
                                                                                                       aaaTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCa                                              <  1:88994/150‑1 (MQ=255)
                                                                                                         aTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAgg                                            >  2:1556846/1‑150 (MQ=255)
                                                                                                               cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTcc                                      <  2:1729799/150‑1 (MQ=255)
                                                                                                                tttAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACTGCCTGAGCAACGTTTCTCGGCGCTTCATCATACTTCAGGAATTACa                                     >  1:1054259/1‑150 (MQ=255)
                                                                                                                   aGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAg                                  >  1:825989/1‑150 (MQ=255)
                                                                                                                   aGACACGGCTATATTCCTTACTATAGTCCTCTCCCCTTCAGGCGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAg                                  <  2:342290/150‑1 (MQ=255)
                                                                                                                   aGACACGGCTATATTCCTTACTATAATGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAg                                  <  2:1687723/150‑1 (MQ=255)
                                                                                                                      cacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAgtgt                               >  2:1847926/1‑150 (MQ=255)
                                                                                                                          gCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTatga                           >  1:1089774/1‑150 (MQ=255)
                                                                                                                                       gtcccgTGCTCTCTCCTGAAGGGGAGAGCACTGGTCTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTTCGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTAAGGAATTCCATAGTGTATGATGCCCGACCtttt              >  1:1726251/6‑149 (MQ=255)
                                                                                                                                          ttgTGCTCTCCCCTTCCGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTc           <  2:1421126/148‑1 (MQ=255)
                                                                                                                                          taGTGCGCACCCCTTCCGCAGCTAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTc           <  1:1569716/150‑1 (MQ=255)
                                                                                                                                          taGTGAGCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAATAACCCTTAGTCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTc           <  2:305390/150‑1 (MQ=255)
                                                                                                                                           cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCa          >  1:913444/2‑150 (MQ=255)
                                                                                                                                           cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCa          >  1:838347/2‑150 (MQ=255)
                                                                                                                                           cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCa          >  2:1267625/2‑150 (MQ=255)
                                                                                                                                            gTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCag         >  1:274272/1‑150 (MQ=255)
                                                                                                                                              gcgctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATAAAGCCTTAGGCTTATTTACCACGGGCTGCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGATTTCCATAGTGTATGATGCACGACCTTTGGTCagag       <  2:107571/147‑1 (MQ=255)
                                                                                                                                              gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCagag       <  2:413638/150‑1 (MQ=255)
                                                                                                                                               ctctCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAgaga      >  1:782614/1‑150 (MQ=255)
                                                                                                                                               ctctCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAgaga      >  1:219922/1‑150 (MQ=255)
                                                                                                                                                 ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAAc    <  2:1089132/150‑1 (MQ=255)
                                                                                                                                                  cccccTGCAGGAGTGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACg   <  2:1862482/149‑1 (MQ=255)
                                                                                                                                                   ccccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCTATTACGGCCTGAGCCACGTTACTCGGCGCTTCATCAGACTTCACGAATTCCATAGTGTATGAGGCACGACCTTTGGACAGAGAACGc  >  1:746283/1‑150 (MQ=255)
                                                                                                                                                   ccccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGc  >  1:1524365/1‑150 (MQ=255)
                                                                                                                                                   ccccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGc  >  2:558454/1‑150 (MQ=255)
                                                                                                                                                   ccccTTCAGGAGAGAGCACGGGACTTTGCTATTAACCCTTAGCCTTATTTACGACGGGCTTCCATTACGGCCTGAGGGACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCAAGACCTATGGTCAGAGAACGc  >  1:739703/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGC  >  NC_000913/3471209‑3471505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: