Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,471,366 A→G 7.7% intergenic (‑37/+34) tufA ← / ← fusA translation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,471,3660AG 7.7% 336.8 / 14.4 120intergenic (‑37/+34)tufA/fusAtranslation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand):  ref base A (61/45);  new base G (5/4);  total (66/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01

Only 100 of 125 total aligned reads displayed.

AGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGT  >  NC_000913/3471219‑3471513
                                                                                                                                                   |                                                                                                                                                   
aGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGACCGATAGTACCAACGTTAAGGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgaga                                                                                                                                                   >  2:88994/1‑150 (MQ=255)
 gCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                                                                                                                  >  1:1530739/1‑150 (MQ=255)
 gCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                                                                                                                  <  1:8554/150‑1 (MQ=255)
 gCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                                                                                                                  >  2:1820323/1‑150 (MQ=255)
  ccAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagaga                                                                                                                                                 >  2:1214157/1‑150 (MQ=255)
    aGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGc                                                                                                                                               >  2:1786811/1‑150 (MQ=255)
      tACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                                                                             <  1:804399/150‑1 (MQ=255)
      tACGGTGGTGATTGCAGCGGGCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                                                                             <  1:1171341/150‑1 (MQ=21)
        cGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACgg                                                                                                                                           <  1:843904/150‑1 (MQ=255)
         ggtggtGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCATCGTGGCCTATAGAACCAACGTTAACGTGCTGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCActat                                                                                                                                          <  1:346643/150‑4 (MQ=255)
           tggtgATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGAc                                                                                                                                        >  1:904136/1‑150 (MQ=255)
            ggtgATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGCTAGTACCAACGTTAACGTGCGGTTTTGTACTTTCAAATTTTTCTTTAGACTCGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGTCt                                                                                                                                       >  1:305390/1‑150 (MQ=255)
             gtgATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACtt                                                                                                                                      >  1:1135047/1‑150 (MQ=255)
               gATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTg                                                                                                                                    >  2:509109/1‑150 (MQ=255)
                 ttGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGt                                                                                                                                  >  1:170416/1‑150 (MQ=255)
                 ttGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGt                                                                                                                                  <  2:1061049/150‑1 (MQ=255)
                   gCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAt                                                                                                                                >  2:1164464/1‑150 (MQ=255)
                   gCAGCGGTCAGAGTAGATTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACAAGGCTATATTCCTTACTATAGTGCTCTCCACTTCAGGAGAGAGCCCGGGACTTAGGTAt                                                                                                                                >  1:318915/1‑150 (MQ=25)
                    cAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAtt                                                                                                                               >  1:1358225/1‑150 (MQ=255)
                          tCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCt                                                                                                                         <  2:1742060/150‑1 (MQ=255)
                           cAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAGCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCtt                                                                                                                        >  2:626982/1‑150 (MQ=255)
                              agtagtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACTTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                                                                                                                     <  2:719841/150‑1 (MQ=255)
                              agtagtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGGGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                                                                                                                     <  2:336466/150‑1 (MQ=255)
                               ttatttttCCCGTTGTCCACGTGGCCTATTTTACAAACGTTAAAGTGCTGTTTTGTCAGTTCAAATTTTTATTTTTACACGGCTATATTCTTTACTTTAGTGCTCTCCCCTTCAGGTGAGAGCACGGGACTTTGGTATTAACCCTTAGGc                                                                                                                    <  1:699210/146‑1 (MQ=255)
                               gtagtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGc                                                                                                                    >  1:1081694/1‑150 (MQ=255)
                                  gtTTTACCGTGGTCAACGTGGCCGATAGTCCAAACGTTCACGTGCGGTTTTGTACGTTCTAAGTTTTCTTTAGACCCGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGCGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                                                                                 >  2:886496/1‑150 (MQ=37)
                                  gtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCTAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                                                                                 >  2:1211052/1‑150 (MQ=255)
                                  gtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                                                                                 >  2:1260761/1‑150 (MQ=255)
                                    tttACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAtt                                                                                                               <  1:105998/150‑1 (MQ=255)
                                       aCCGTGGGCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                                                                            <  1:1250225/150‑1 (MQ=255)
                                        ccGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAcc                                                                                                           >  1:657031/1‑150 (MQ=255)
                                        ccGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAcc                                                                                                           >  1:1592641/1‑150 (MQ=255)
                                          gTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCAc                                                                                                         <  1:261475/150‑1 (MQ=255)
                                            ggTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTACTTACTATAGTGCTCTCCCCTGCAGGAGAGAGCACGGGACTTTAGTATTAACCCTTAGGCTTATTTACCACgg                                                                                                       >  1:1067846/1‑150 (MQ=255)
                                              tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACgggg                                                                                                     >  2:1233326/1‑149 (MQ=255)
                                               aaaggTGGCCTATAGACCCAAGTTAAAGGTGCGGTTTTGAACTTTAAAATTTTTTTTTTTCAACGGTATTTTTCCTTATTATTGTGCTCCCCCATTCCGGAGATAGAACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCt                                                                                                    <  2:1434758/146‑1 (MQ=255)
                                                aaCGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGCACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCtt                                                                                                   >  2:1770907/1‑150 (MQ=255)
                                                   gTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                                                                                >  1:150455/1‑150 (MQ=255)
                                                      gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTa                                                                                             >  1:1227863/1‑150 (MQ=255)
                                                      gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTa                                                                                             >  1:1862482/1‑150 (MQ=255)
                                                        cGATAGTACCAACGTTAACGTGCGGTTTTGTACCGTCAAATATTGCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACg                                                                                           <  2:1131574/150‑1 (MQ=255)
                                                         gATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACgg                                                                                          <  2:1600171/150‑1 (MQ=255)
                                                          aTAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGTAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGc                                                                                         <  1:209670/150‑1 (MQ=255)
                                                            aGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCt                                                                                       <  2:515365/150‑1 (MQ=255)
                                                              tACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTgg                                                                                     >  2:1011255/1‑149 (MQ=255)
                                                                ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGc                                                                                   >  2:1613396/1‑150 (MQ=255)
                                                                 cAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCa                                                                                  >  2:446786/1‑150 (MQ=255)
                                                                      ttAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGtt                                                                             <  2:1306507/150‑1 (MQ=255)
                                                                       tAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTTCTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTa                                                                            <  2:70504/150‑1 (MQ=255)
                                                                           gTGCGGTTTTGTACGTTCAAATTTTTGTTTAGACACAGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGCTAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCg                                                                        <  1:515420/150‑1 (MQ=255)
                                                                             gCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc                                                                      >  1:624416/1‑150 (MQ=255)
                                                                             gCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAACACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc                                                                      >  1:1350120/1‑150 (MQ=255)
                                                                              cGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACTGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg                                                                     >  1:237799/1‑150 (MQ=255)
                                                                                gTTTTGTACGTTCAAATTTTTCTTTAGACTCGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt                                                                   >  1:399940/1‑150 (MQ=255)
                                                                                 ttttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCtt                                                                  <  2:853618/150‑1 (MQ=255)
                                                                                   ttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtca                                                                <  2:1020415/150‑1 (MQ=255)
                                                                                    tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcat                                                               <  2:332268/150‑1 (MQ=255)
                                                                                     gTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc                                                              <  1:46086/150‑1 (MQ=255)
                                                                                     gTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc                                                              <  2:1135047/150‑1 (MQ=255)
                                                                                      tACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatca                                                             >  2:1017811/1‑150 (MQ=255)
                                                                                          ttCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACt                                                         >  1:1593133/1‑150 (MQ=255)
                                                                                            cAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTc                                                       <  2:336749/150‑1 (MQ=255)
                                                                                             aaaTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCa                                                      <  1:88994/150‑1 (MQ=255)
                                                                                               aTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAgg                                                    >  2:1556846/1‑150 (MQ=255)
                                                                                                     cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTcc                                              <  2:1729799/150‑1 (MQ=255)
                                                                                                      tttAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACTGCCTGAGCAACGTTTCTCGGCGCTTCATCATACTTCAGGAATTACa                                             >  1:1054259/1‑150 (MQ=255)
                                                                                                         aGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAg                                          >  1:825989/1‑150 (MQ=255)
                                                                                                         aGACACGGCTATATTCCTTACTATAGTCCTCTCCCCTTCAGGCGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAg                                          <  2:342290/150‑1 (MQ=255)
                                                                                                         aGACACGGCTATATTCCTTACTATAATGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAg                                          <  2:1687723/150‑1 (MQ=255)
                                                                                                            cacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAgtgt                                       >  2:1847926/1‑150 (MQ=255)
                                                                                                            cacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAgtgt                                       >  1:908606/1‑150 (MQ=255)
                                                                                                            cacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAgtgt                                       >  2:1828103/1‑150 (MQ=255)
                                                                                                                  tataTTCCTTAAAATAGGGCTCTCCCCTTCCGGCTATAGAACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTatgatg                                 <  2:1375483/150‑1 (MQ=255)
                                                                                                                      ttCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACg                             <  1:1214157/150‑1 (MQ=255)
                                                                                                                             gtcccgTGCTCTCTCCTGAAGGGGAGAGCACTGGTCTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTTCGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTAAGGAATTCCATAGTGTATGATGCCCGACCtttt                      >  1:1726251/6‑149 (MQ=255)
                                                                                                                                ttgTGCTCTCCCCTTCCGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTc                   <  2:1421126/148‑1 (MQ=255)
                                                                                                                                taGTGCGCACCCCTTCCGCAGCTAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTc                   <  1:1569716/150‑1 (MQ=255)
                                                                                                                                taGTGAGCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAATAACCCTTAGTCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTc                   <  2:305390/150‑1 (MQ=255)
                                                                                                                                ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTc                   >  2:598269/3‑150 (MQ=255)
                                                                                                                                 cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCa                  >  1:838347/2‑150 (MQ=255)
                                                                                                                                 cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCa                  >  2:1267625/2‑150 (MQ=255)
                                                                                                                                    gcgctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATAAAGCCTTAGGCTTATTTACCACGGGCTGCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGATTTCCATAGTGTATGATGCACGACCTTTGGTCagag               <  2:107571/147‑1 (MQ=255)
                                                                                                                                    gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCagag               <  2:413638/150‑1 (MQ=255)
                                                                                                                                    gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCagag               <  2:1081694/150‑1 (MQ=255)
                                                                                                                                     ctctCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAgaga              >  1:219922/1‑150 (MQ=255)
                                                                                                                                     ctctCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAgaga              >  1:782614/1‑150 (MQ=255)
                                                                                                                                       ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAAc            <  2:1089132/150‑1 (MQ=255)
                                                                                                                                         ccccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCTATTACGGCCTGAGCCACGTTACTCGGCGCTTCATCAGACTTCACGAATTCCATAGTGTATGAGGCACGACCTTTGGACAGAGAACGc          >  1:746283/1‑150 (MQ=255)
                                                                                                                                         ccccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGc          >  1:1524365/1‑150 (MQ=255)
                                                                                                                                         ccccTTCAGGAGAGAGCACGGGACTTTGCTATTAACCCTTAGCCTTATTTACGACGGGCTTCCATTACGGCCTGAGGGACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCAAGACCTATGGTCAGAGAACGc          >  1:739703/1‑150 (MQ=255)
                                                                                                                                           ccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGATTTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAg        >  1:1819326/1‑150 (MQ=255)
                                                                                                                                           ccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAg        >  1:1353718/1‑150 (MQ=255)
                                                                                                                                             ttCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCt      <  2:904136/150‑1 (MQ=255)
                                                                                                                                              tCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCATTTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTg     <  2:318915/150‑1 (MQ=255)
                                                                                                                                              tCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTg     <  2:193655/150‑1 (MQ=255)
                                                                                                                                              tCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTg     <  1:921700/150‑1 (MQ=255)
                                                                                                                                                cggAGATAGAACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAg   <  1:1820323/149‑1 (MQ=255)
                                                                                                                                                aGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAg   <  1:1521002/150‑1 (MQ=255)
                                                                                                                                                aGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAg   >  2:1539341/1‑150 (MQ=255)
                                                                                                                                                 ggAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGt  >  2:208841/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGT  >  NC_000913/3471219‑3471513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: