Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,477,341 C→A 5.1% E27* (GAA→TAA) ‡ fkpA ← FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,477,3410CA 5.1% 347.5 / 5.7 111E27* (GAA→TAA) ‡fkpAFKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base C (44/61);  new base A (2/4);  total (46/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 121 total aligned reads displayed.

TTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTC  >  NC_000913/3477192‑3477490
                                                                                                                                                     |                                                                                                                                                     
tttATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTc                                                                                                                                                       >  1:1417623/1‑150 (MQ=255)
 ttATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTca                                                                                                                                                      <  2:609122/150‑1 (MQ=255)
   aTCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTcagc                                                                                                                                                    >  2:879941/1‑150 (MQ=255)
   aTCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTcacc                                                                                                                                                    >  2:780955/1‑148 (MQ=255)
      cAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTTCAGCTTCagcagc                                                                                                                                                 >  2:230976/1‑150 (MQ=255)
      cAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCagcagc                                                                                                                                                 <  1:1363740/150‑1 (MQ=255)
            gATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCTGCTGAAGCTGCaaaacc                                                                                                                                           <  1:393198/150‑3 (MQ=255)
             ttGCCCTGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTTCTGTCAGCAGCTGTAGCAGGTTTTGCTGCTGAAGCTGCaaaacct                                                                                                                                          <  2:20220/149‑4 (MQ=255)
             aTGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCTGCTGAAGCTGCaaaacct                                                                                                                                          <  2:625620/150‑4 (MQ=255)
             aTGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTg                                                                                                                                          <  1:1812801/150‑1 (MQ=255)
               gCCCAGTTTTTCTTGTTCTTTTAGCGAGTTTTCCATGTACCGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCTGCTGAAGCTGCaaaacctgc                                                                                                                                        <  2:335648/150‑6 (MQ=255)
               gCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTTCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGAt                                                                                                                                        <  2:1216980/150‑1 (MQ=255)
               gCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTCGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGAt                                                                                                                                        >  2:1726999/1‑150 (MQ=255)
                 ccAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTg                                                                                                                                      <  2:1093332/150‑1 (MQ=255)
                  cAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCAAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTgg                                                                                                                                     >  1:829600/1‑150 (MQ=255)
                   aGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGt                                                                                                                                    >  1:646867/1‑150 (MQ=255)
                     tttttCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGAAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGtgc                                                                                                                                  >  1:620911/1‑150 (MQ=255)
                          cTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGtgcatgca                                                                                                                             >  2:555326/1‑150 (MQ=255)
                           ttGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAg                                                                                                                            >  1:729736/1‑150 (MQ=255)
                           ttGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAg                                                                                                                            <  1:869936/150‑1 (MQ=255)
                           ttGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGAAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAg                                                                                                                            >  1:723108/1‑150 (MQ=255)
                            tGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAgg                                                                                                                           <  1:483311/150‑1 (MQ=255)
                             gTTCTTTTAGAGAGTTTTCCATGTAACGCCCCAGCGAGGCACCCAGTGCTTAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAggg                                                                                                                          >  2:23209/1‑150 (MQ=255)
                             gTTCATTTTGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCATCTTCAGCAGCAAAAGTGTTTTGTGCATGCAggg                                                                                                                          <  1:1771488/150‑1 (MQ=255)
                                 ttttAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAAc                                                                                                                      >  1:1763784/1‑150 (MQ=255)
                                  tttAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACg                                                                                                                     >  1:193553/1‑150 (MQ=255)
                                   ttAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACgg                                                                                                                    <  1:1320183/150‑1 (MQ=255)
                                    tAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGc                                                                                                                   <  2:1784275/150‑1 (MQ=255)
                                    tAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGc                                                                                                                   >  2:1400971/1‑150 (MQ=255)
                                    tAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGc                                                                                                                   >  2:1372517/1‑150 (MQ=255)
                                        gagTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTGATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCCGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCAtt                                                                                                               >  2:765789/1‑150 (MQ=255)
                                        gagTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCAtt                                                                                                               <  2:731549/150‑1 (MQ=255)
                                        gagTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCAtt                                                                                                               >  2:1068279/1‑150 (MQ=255)
                                         agTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATtg                                                                                                              <  2:646867/150‑1 (MQ=255)
                                          gTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGTTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATtgt                                                                                                             <  1:1456240/150‑1 (MQ=255)
                                           ttttCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATtgtg                                                                                                            <  1:1311413/150‑1 (MQ=255)
                                            tttCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTgg                                                                                                           >  1:953627/1‑150 (MQ=255)
                                            tttCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTgg                                                                                                           <  1:1067605/150‑1 (MQ=255)
                                            tttCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGGAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTgg                                                                                                           <  1:1641113/150‑1 (MQ=255)
                                              tCCATGTAACGACCCAGCGAGGCCCCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTc                                                                                                         >  1:747390/1‑150 (MQ=255)
                                               ccATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCg                                                                                                        <  1:473111/150‑1 (MQ=255)
                                               ccAAGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCg                                                                                                        <  1:513845/150‑1 (MQ=255)
                                                cATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTAGc                                                                                                       >  2:523905/1‑150 (MQ=255)
                                                cATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTAGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGc                                                                                                       >  2:129937/1‑150 (MQ=255)
                                                  tGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCca                                                                                                     <  1:977244/150‑1 (MQ=255)
                                                    tAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCcagc                                                                                                   <  2:872085/150‑1 (MQ=255)
                                                       cGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCagcagc                                                                                                <  2:878282/150‑1 (MQ=255)
                                                        gACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGGGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCg                                                                                               <  1:1009708/150‑1 (MQ=255)
                                                          cccAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGtt                                                                                             >  1:1176080/1‑150 (MQ=255)
                                                            cAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTAc                                                                                           >  1:1874715/1‑150 (MQ=255)
                                                             aGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACt                                                                                          <  2:438469/150‑1 (MQ=255)
                                                              gCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACtt                                                                                         <  2:900590/150‑1 (MQ=255)
                                                                gAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTa                                                                                       <  1:1518582/150‑1 (MQ=255)
                                                                 cggCACCCAGTGCAGAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGGTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTaa                                                                                      <  2:1409154/149‑1 (MQ=255)
                                                                        cAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGa                                                                               <  1:1261000/150‑1 (MQ=255)
                                                                          gTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGGAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGAtt                                                                             <  1:270221/150‑1 (MQ=255)
                                                                          gTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGAtt                                                                             <  2:1241751/150‑1 (MQ=255)
                                                                            gCATAAGCTGATTTCTGATCGTCATTTTTGCACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTc                                                                           <  2:1401386/150‑1 (MQ=255)
                                                                            gCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTc                                                                           <  1:1503901/150‑1 (MQ=255)
                                                                            gCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTc                                                                           <  2:1393542/150‑1 (MQ=255)
                                                                             cATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCa                                                                          <  1:1840800/150‑1 (MQ=255)
                                                                             cATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCa                                                                          <  2:1806315/150‑1 (MQ=255)
                                                                                   cTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCata                                                                    <  1:681472/150‑1 (MQ=255)
                                                                                        ttCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCAATATCTcc                                                               <  2:1417623/150‑1 (MQ=255)
                                                                                          cTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAg                                                             >  2:1275481/1‑150 (MQ=255)
                                                                                          cTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAg                                                             <  1:356684/150‑1 (MQ=255)
                                                                                            gATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCCACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGa                                                           >  2:1350085/1‑150 (MQ=255)
                                                                                               cGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCg                                                        >  1:374669/1‑150 (MQ=255)
                                                                                                gTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCgg                                                       >  2:1023468/1‑150 (MQ=255)
                                                                                                gTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCgg                                                       <  1:3219/150‑1 (MQ=255)
                                                                                                 tCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCggg                                                      <  2:1512730/150‑1 (MQ=255)
                                                                                                  cATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCgggg                                                     <  1:464403/150‑1 (MQ=255)
                                                                                                  cATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCgggg                                                     <  2:193553/150‑1 (MQ=255)
                                                                                                   aTTTTTGACCGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGc                                                    <  2:762898/150‑1 (MQ=255)
                                                                                                   aTTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGc                                                    >  1:175517/1‑150 (MQ=255)
                                                                                                    tttttGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTTGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCa                                                   <  2:1219990/150‑1 (MQ=255)
                                                                                                    tttttGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCTACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCa                                                   >  1:1771272/1‑150 (MQ=255)
                                                                                                    tttttGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCa                                                   <  1:269009/150‑1 (MQ=255)
                                                                                                            cGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCCTCCATATCTCCAGGATCGCGGCATCTCAccc                                           >  1:678401/1‑150 (MQ=255)
                                                                                                               tgctTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGAGGCATCTCACCCCAg                                        <  2:375397/150‑1 (MQ=255)
                                                                                                                 ctTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAggg                                      <  1:386797/150‑1 (MQ=255)
                                                                                                                  tttGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGt                                     <  1:1391864/150‑1 (MQ=255)
                                                                                                                     gCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTaa                                  >  2:1326220/1‑150 (MQ=255)
                                                                                                                           agcagcTGTAGCAGGTTTTGCCGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACtattt                            >  1:76305/1‑149 (MQ=255)
                                                                                                                           agcagcTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTTTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCCTCCATATCTCCAGGATCGGGGCATCTCCCCCCAGGGTTAACtatta                            >  1:889707/1‑150 (MQ=255)
                                                                                                                              agcTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCa                         <  2:1076578/150‑1 (MQ=255)
                                                                                                                               gcTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAACCAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTAGTATCaa                        >  1:1658698/1‑149 (MQ=255)
                                                                                                                                    aatcacttttGCTGCTGAAGCTGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACACCAGGGTTAACTATTATCAGAAggg                   >  1:368001/7‑150 (MQ=255)
                                                                                                                                    aGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAggg                   <  1:9528/150‑1 (MQ=255)
                                                                                                                                     gCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGt                  <  1:251016/150‑1 (MQ=255)
                                                                                                                                     gCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGt                  <  1:755447/150‑1 (MQ=255)
                                                                                                                                        ggTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACt               <  2:58179/150‑1 (MQ=255)
                                                                                                                                             tGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATaaa          >  1:190616/1‑150 (MQ=255)
                                                                                                                                                 gCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGt      <  1:436261/150‑1 (MQ=255)
                                                                                                                                                 gCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGt      >  2:769870/1‑150 (MQ=255)
                                                                                                                                                  cTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGtt     <  2:762523/150‑1 (MQ=255)
                                                                                                                                                  cTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGtt     <  2:1204658/150‑1 (MQ=255)
                                                                                                                                                     cagcagCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTc  <  1:230976/150‑1 (MQ=255)
                                                                                                                                                     cagcagCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTc  >  1:1079787/1‑150 (MQ=255)
                                                                                                                                                     cagcagCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGAACGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTc  <  2:1571274/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTATCCAGTTTGATGCCCAGTTTTTCTTGTTCTTTTAGAGAGTTTTCCATGTAACGACCCAGCGAGGCACCCAGTGCATAAGCTGATTTCTGATCGTCATTTTTGAACGCTGCTTTGCTGTCAGCAGCTGTAGCAGGTTTTGCAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTC  >  NC_000913/3477192‑3477490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: