Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,483,244 A→G 6.2% S15G (AGT→GGT) ‡ yheT → UPF0017 family putative hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,483,2440AG 6.2% 341.0 / 9.7 115S15G (AGT→GGT) ‡yheTUPF0017 family putative hydrolase
Reads supporting (aligned to +/- strand):  ref base A (47/60);  new base G (2/5);  total (49/66)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

Only 100 of 124 total aligned reads displayed.

CTGCAACAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGTGAA  >  NC_000913/3483095‑3483393
                                                                                                                                                     |                                                                                                                                                     
 tgcAACAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTcagcagcag                                                                                                                                                      >  1:400622/1‑150 (MQ=255)
 tccAACAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTcagcagcag                                                                                                                                                      >  2:1387369/3‑150 (MQ=255)
   cAACAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTg                                                                                                                                                    >  2:287624/1‑150 (MQ=255)
     aCAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCCAAATGGCATGGCTGGAAGCCCAGGAGCAGCTAGAGCAGATGCTGCAGGAAGGACAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCt                                                                                                                                                  >  2:1281708/1‑150 (MQ=255)
      cAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTg                                                                                                                                                 <  1:787143/150‑1 (MQ=255)
             ccAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCACTGCTGCTGAATTCAt                                                                                                                                          <  1:587853/150‑1 (MQ=255)
              cAGCGCCAAATCCGGCCTGGAAGAGTGCTATATCGCAAGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATc                                                                                                                                         <  1:270743/150‑1 (MQ=255)
              cAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAACGCAACTGATGGAGCAGATAACGACGACCGATGCCAATGAATACAGCAGCAGTGCTGAATTCCTc                                                                                                                                         >  1:1589714/1‑150 (MQ=255)
              cAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATc                                                                                                                                         <  2:612800/150‑1 (MQ=255)
              cAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATc                                                                                                                                         <  1:1306082/150‑1 (MQ=255)
              cAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATc                                                                                                                                         >  2:686600/1‑150 (MQ=255)
              cAGCGCCAAATCCGGCCTGGAAGAGTGCGAAAGGGCATGGCTGGAAGCCCAGGAGCTGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCACTGCTGCTGAATTCAtt                                                                                                                                         <  2:449969/150‑2 (MQ=255)
               aGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCACTGCTGCTGAATTCAttg                                                                                                                                        <  1:1085/150‑3 (MQ=255)
               aGCGCCAAATCCGGCCGGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATcc                                                                                                                                        <  2:1653862/150‑1 (MQ=255)
                 cgcCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATcccc                                                                                                                                      <  2:1694148/150‑1 (MQ=255)
                  gcCAAATCCGGCCTGGAAGAGTGCGACATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCa                                                                                                                                     <  1:1206373/150‑1 (MQ=255)
                    cAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTAATCCCCATg                                                                                                                                   >  1:1648521/1‑150 (MQ=255)
                          cGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGc                                                                                                                             >  2:1308745/1‑150 (MQ=255)
                          cGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGc                                                                                                                             >  2:19593/1‑150 (MQ=255)
                             ccTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCTAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCttt                                                                                                                          >  1:1668157/1‑150 (MQ=255)
                             ccTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCttt                                                                                                                          <  2:36167/150‑1 (MQ=255)
                              cTGGATGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTa                                                                                                                         <  1:1475438/150‑1 (MQ=255)
                              cTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGCATTCATCCCCATGCGCGGCTTTa                                                                                                                         >  2:1304764/1‑150 (MQ=255)
                              cTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTa                                                                                                                         >  1:1625592/1‑150 (MQ=255)
                              cTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTa                                                                                                                         >  1:105834/1‑150 (MQ=255)
                                   agagTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAAt                                                                                                                    <  1:203848/150‑1 (MQ=255)
                                    gagTGCGAAATGGCATGGCTGGAAGCCCTGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCGGAATTCATCCCCATGCGCGGCTTTAGCAAtt                                                                                                                   <  1:1123611/150‑1 (MQ=255)
                                     tgTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCACTGCTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTg                                                                                                                  <  1:1629097/149‑1 (MQ=255)
                                       tGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGGTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTc                                                                                                                <  2:448677/150‑1 (MQ=255)
                                       tGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCAAAAGCAACTGATGGCGCTGATAACGACGACCGATGCCAATTAATTAAGCAGCAGTGCTGAAATCATCCCCATGCGCGGCTTTAGCAATTGTc                                                                                                                >  2:312041/1‑150 (MQ=255)
                                        gCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATACCCATGCGCGGCTTTAGCAATTGTCa                                                                                                               >  1:872544/1‑150 (MQ=255)
                                          taaaTGTCATGGCTGTAAGCCCAGGAGCAGCTTGAGCAGATTCTGCTGGAAGTCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGATTTCAGAAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCAta                                                                                                             <  1:1429579/149‑2 (MQ=255)
                                          gAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATc                                                                                                             >  1:1578833/1‑150 (MQ=255)
                                          gAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATc                                                                                                             <  1:958245/150‑1 (MQ=255)
                                          gAAATGGCATGGCCGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATc                                                                                                             <  1:845402/150‑1 (MQ=255)
                                           aaaTGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCCATGAATTCAGCAGCAGTGCTGAATTCATCCCCAGGCGCGGCTTTAGCAATTGTCATcg                                                                                                            >  2:706165/1‑149 (MQ=255)
                                           aaaTGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCt                                                                                                            <  1:93410/150‑1 (MQ=255)
                                            aaTGGCTTGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGTAGGCCAAAGCAACTGATGGCGCAGATTACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTg                                                                                                           <  2:1497979/150‑1 (MQ=255)
                                                 catggcTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAAc                                                                                                      >  1:51579/1‑150 (MQ=255)
                                                  atggcTGGAAGCCCAGGAGCAGCTTGAGCCGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGTTAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAAcc                                                                                                     <  1:919798/150‑1 (MQ=255)
                                                   tggcGGGAAGCCCAGGAGCTGCTTGAGCAGATGCTGCTGGAAGGCCAAATCAACTGATGGGGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCa                                                                                                    <  2:1144043/150‑1 (MQ=255)
                                                     gcTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCAtg                                                                                                  >  2:791238/1‑150 (MQ=255)
                                                     gcTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCAtg                                                                                                  >  2:167007/1‑150 (MQ=255)
                                                      cTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCAtgc                                                                                                 >  1:255949/1‑150 (MQ=255)
                                                       tGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCAtgct                                                                                                <  1:1615933/150‑1 (MQ=255)
                                                        ggAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCAtgctg                                                                                               >  1:1774430/1‑150 (MQ=255)
                                                            gCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCcgc                                                                                           <  1:168932/150‑1 (MQ=255)
                                                             cccAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCcgcg                                                                                          <  1:601446/150‑1 (MQ=255)
                                                             cccAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGAGGCTTTTGCAATTGTCATCTGGATAGCATGCTGCcgcg                                                                                          >  2:911997/1‑150 (MQ=255)
                                                               cAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCCTGCTGCCGCGTc                                                                                        >  1:1358979/1‑150 (MQ=255)
                                                                cggAGCAGCTTGAGCAGATGCTGCCGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCt                                                                                       <  2:344140/149‑1 (MQ=255)
                                                                aGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCt                                                                                       <  1:1113011/150‑1 (MQ=255)
                                                                aGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCt                                                                                       <  1:878540/150‑1 (MQ=255)
                                                                    gcagcTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGttt                                                                                   <  1:572082/150‑1 (MQ=255)
                                                                      agcTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTtcg                                                                                 <  1:869416/150‑1 (MQ=255)
                                                                      agcTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGACGCAGATAACGACGACCGATGCAAATGAATTCAGCACCAGTGCTGAAATAATACCCATGCGCGGCTTTAGGAATTGTCATCTGCCAACCATGCTGCCGCGTGTGTTtcg                                                                                 >  2:80603/1‑150 (MQ=255)
                                                                         ttGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTtcgtcg                                                                              <  1:686600/150‑1 (MQ=255)
                                                                         ttGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTATAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTtcgtcg                                                                              >  1:120648/1‑150 (MQ=255)
                                                                           taGCAGATGCTGCTGTAAGGCCAAAGCAACTGATTGCGCAGATAACGACGACCTATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCgtcgtc                                                                            <  2:557596/149‑1 (MQ=255)
                                                                           gAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCgtcgtc                                                                            <  1:890337/150‑1 (MQ=255)
                                                                             gCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAg                                                                          >  2:618345/1‑150 (MQ=255)
                                                                                gATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTg                                                                       <  2:465767/150‑1 (MQ=255)
                                                                                gATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTg                                                                       <  1:377134/150‑1 (MQ=255)
                                                                                gATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTg                                                                       <  1:1020860/150‑1 (MQ=255)
                                                                                 aTGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAGTTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGa                                                                      <  2:1282614/150‑1 (MQ=255)
                                                                                  tgctgcTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATATGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGaa                                                                     >  1:556807/1‑150 (MQ=255)
                                                                                   gctgctGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGaaa                                                                    <  1:287624/150‑1 (MQ=255)
                                                                                     tgctgGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAAtt                                                                  >  2:543199/1‑150 (MQ=255)
                                                                                      gctgGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGGCTGTTTCGTCGTCAGGTGAAATTc                                                                 >  2:1526381/1‑150 (MQ=255)
                                                                                         ggAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCAcc                                                              <  2:1483099/150‑1 (MQ=255)
                                                                                         ggAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCAcc                                                              >  1:1654652/1‑150 (MQ=255)
                                                                                          gAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGGAATTCAccc                                                             <  2:205645/150‑1 (MQ=255)
                                                                                            aGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCg                                                           <  2:1874065/150‑1 (MQ=255)
                                                                                             ggCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGt                                                          >  2:652301/1‑150 (MQ=255)
                                                                                                      aaCTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGc                                                 <  1:167007/150‑1 (MQ=255)
                                                                                                          gATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCt                                             >  1:635737/1‑150 (MQ=255)
                                                                                                             ggCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGa                                          <  1:1627993/150‑1 (MQ=255)
                                                                                                             ggCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGa                                          <  1:1052762/150‑1 (MQ=255)
                                                                                                              gcgcAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAg                                         <  1:100287/150‑1 (MQ=255)
                                                                                                              gcgcAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAg                                         <  1:19593/150‑1 (MQ=255)
                                                                                                               cgcAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGt                                        <  1:1387369/150‑1 (MQ=255)
                                                                                                                gcAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGtt                                       >  1:413568/1‑150 (MQ=255)
                                                                                                                  aGATACCGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGc                                     <  2:1177623/150‑1 (MQ=255)
                                                                                                                   gATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGcc                                    <  2:105834/150‑1 (MQ=255)
                                                                                                                      aaCGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGa                                 <  2:1061885/150‑1 (MQ=255)
                                                                                                                       acgacgACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGAc                                >  2:809031/1‑150 (MQ=255)
                                                                                                                        cgacgaCCGATGCCAATGAATTCAGCAGCTGTGCTGAATTCTTCCCCATGCGCGGCTTTAGCTATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCAGGCCGTTCTGGCAGCGGCTGGAGTTGCCCGACg                               <  1:1281708/150‑1 (MQ=255)
                                                                                                                                 aTGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGAACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTg                      <  2:1801097/150‑1 (MQ=255)
                                                                                                                                 aTGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGGCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTg                      >  1:633843/1‑150 (MQ=255)
                                                                                                                                  tGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGt                     >  1:1794589/1‑150 (MQ=255)
                                                                                                                                   gCCAATGAATTCAGCAGCATTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTc                    <  1:512846/150‑1 (MQ=255)
                                                                                                                                      aaTGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGAt                 >  1:849344/1‑150 (MQ=255)
                                                                                                                                        tGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCt               <  2:660650/150‑1 (MQ=255)
                                                                                                                                        tGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCt               <  2:1774430/150‑1 (MQ=255)
                                                                                                                                        tGAATTCAGCACTGCTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCt               >  1:1563087/1‑150 (MQ=255)
                                                                                                                                         gAATTCAGCACTGCTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCtt              >  1:1283037/1‑150 (MQ=255)
                                                                                                                                            ttCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCg           >  2:460644/1‑150 (MQ=255)
                                                                                                                                              cagcagcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTg         <  1:1423573/150‑1 (MQ=255)
                                                                                                                                              cagcagcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTg         >  1:1549416/1‑150 (MQ=255)
                                                                                                                                              cagcagcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGAGTTTGTCGATCTTGCGTg         >  2:158188/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGCAACAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGTGAA  >  NC_000913/3483095‑3483393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: