Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,486,217 A→C 5.5% Q33P (CAG→CCG)  crp → cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,486,2170AC 5.5% 329.7 / 4.8 111Q33P (CAG→CCG) crpcAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base A (43/61);  new base C (2/5);  total (45/66)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

ACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAAC  >  NC_000913/3486068‑3486366
                                                                                                                                                     |                                                                                                                                                     
aCAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGGGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTGCTAGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCa                                                                                                                                                       <  2:229271/150‑1 (MQ=255)
 cAGCGGCGTTATCTGGCTCTGTAGAAAGCTTATAACAGAGGATAACCTCGCATGTTGCTTGGCAAACCGCAAACAGACCCGACGCGCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAg                                                                                                                                                      <  2:1762733/150‑1 (MQ=255)
 cAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAg                                                                                                                                                      >  2:743747/1‑150 (MQ=255)
 cAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAg                                                                                                                                                      >  1:1865922/1‑150 (MQ=255)
 cAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGAAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAg                                                                                                                                                      <  1:1183596/150‑1 (MQ=255)
   gcggcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAggg                                                                                                                                                    >  2:1107455/1‑150 (MQ=255)
      gcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGa                                                                                                                                                 <  2:1127148/150‑1 (MQ=255)
      gcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGa                                                                                                                                                 <  2:626226/150‑1 (MQ=255)
       cgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaa                                                                                                                                                >  1:439079/1‑150 (MQ=255)
        gTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTTGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaa                                                                                                                                               <  1:291615/150‑1 (MQ=255)
         ttATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaa                                                                                                                                              <  2:426571/150‑1 (MQ=255)
             cTGGCTGTGGAGAAAGATTATAACAGAGGATATCCGCGCTTGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCg                                                                                                                                          <  1:487641/150‑1 (MQ=255)
             cTGGCTCTGGATAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCg                                                                                                                                          <  1:1665433/150‑1 (MQ=255)
             cTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCg                                                                                                                                          <  2:1085005/150‑1 (MQ=255)
               ggCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGa                                                                                                                                        <  2:1462112/150‑1 (MQ=255)
                gCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTTTTCACCCTGGTGAATAAGCgtgc                                                                                                                                       <  1:720274/150‑4 (MQ=255)
                 ctctGGATAAAGCTTATAACAGATGATAACCGCTCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGTTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTTTTCACCCTGGTGAATAAGCgtgct                                                                                                                                      <  2:223862/150‑5 (MQ=255)
                 ctctGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGaaa                                                                                                                                      >  1:1289492/1‑150 (MQ=255)
                 ctctGGAGAAAGCCTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGaaa                                                                                                                                      <  2:1314077/150‑1 (MQ=255)
                  tctGGAGAAAGCTTATAACAGAGGATAACCGTGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAAc                                                                                                                                     >  2:781409/1‑150 (MQ=255)
                     ggAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCt                                                                                                                                  >  2:1260036/1‑150 (MQ=255)
                       agaAAGCTTATAACAGAGGTTAACCGCGCTTGGTGCTTGGCCACCCGCCAACAGACCCGTCTCTCGAAGGGTTCTTGTCTCATTGCCACATTCAGAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGCAAACGCGGAACCGCTGt                                                                                                                                >  2:55938/1‑150 (MQ=255)
                       agaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGt                                                                                                                                <  1:750278/150‑1 (MQ=255)
                       agaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGt                                                                                                                                <  2:839706/150‑1 (MQ=255)
                       agaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAAAGGTGCTTGACTCATTGCCACATTCAGAAGTACCCATCCAAGAGCACGCTTATTCAACAGGGTGAAAAAGCGGAATCGcact                                                                                                                                >  1:1274620/1‑147 (MQ=255)
                        gaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGta                                                                                                                               >  1:1122714/1‑150 (MQ=255)
                             cTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACa                                                                                                                          >  2:1776613/1‑150 (MQ=255)
                              ttATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACAt                                                                                                                         <  2:582338/150‑1 (MQ=255)
                                 aaaCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGt                                                                                                                      <  1:599761/149‑1 (MQ=255)
                                     agagGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGAGTGAAAAAGCGGAAACGCTGTACTACATCGTTaaa                                                                                                                  >  1:5397/1‑150 (MQ=255)
                                      gagGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAg                                                                                                                 >  2:698350/1‑150 (MQ=255)
                                      gagGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCAACATTCATAAGTACCCATCCAAGATCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTAAATCGTTAAAg                                                                                                                 >  1:1128277/1‑150 (MQ=255)
                                       agcaTAACCGCGCAGGGGGCTTTGCAAACCGCAAACAGACCAGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCTAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaaatg                                                                                                                <  2:321738/147‑3 (MQ=255)
                                        ggATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCAGCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGc                                                                                                               >  1:57730/1‑150 (MQ=255)
                                              ccGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCAATGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgtg                                                                                                         <  2:221660/150‑1 (MQ=255)
                                                     tGGTGCTTGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCTTTAAAGTCTCTGTGGCAGTGc                                                                                                  >  2:692449/1‑150 (MQ=255)
                                                     tGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGc                                                                                                  <  1:1701239/150‑1 (MQ=255)
                                                     tGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGc                                                                                                  >  1:663347/1‑150 (MQ=255)
                                                     tGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGc                                                                                                  >  1:1750552/1‑150 (MQ=255)
                                                      ggTGCTTGGCAGACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCt                                                                                                 <  2:1065524/150‑1 (MQ=255)
                                                       gTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTG‑AAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGa                                                                                               <  2:722994/150‑1 (MQ=255)
                                                        tGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCCCAAGGGTGAAAAAGCGGAAAAGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGa                                                                                               >  1:81821/1‑150 (MQ=255)
                                                        tGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGa                                                                                               >  1:701782/1‑150 (MQ=255)
                                                         gCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGAt                                                                                              <  1:1398211/150‑1 (MQ=255)
                                                          cTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATc                                                                                             <  2:60427/150‑1 (MQ=255)
                                                              gCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAg                                                                                         <  1:1043355/150‑1 (MQ=255)
                                                                aaaCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGAc                                                                                       <  1:1574880/150‑1 (MQ=255)
                                                                   ccGCAAACAGACCCGACTCTCGAATGGTTATTGTCTCATTGCCACAGTCATACGTACCCCTCCAAGAGCACGCTTATTCACCAGGGGGAAAAAGCGGCAACGCTGTCCTACATCGGTAAAGGCTCTGTGGCTGTGCTGATCAAAGACGaa                                                                                    >  1:180591/1‑150 (MQ=255)
                                                                      cAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAagag                                                                                 >  2:1732649/1‑150 (MQ=255)
                                                                        aaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAggg                                                                               <  1:587684/150‑1 (MQ=255)
                                                                          cAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTa                                                                             >  2:482411/1‑150 (MQ=255)
                                                                          cAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGGTAAAGGCTCTGTGGCTGTGCTGATCAAAGACGAAGAGGGTa                                                                             >  2:714258/1‑150 (MQ=255)
                                                                              cgcGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAATAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGa                                                                         <  1:1767350/148‑1 (MQ=255)
                                                                              cccGACTCTCGAATGGTTCTTGTCTCTTTGACACATTCTTAAGTACCCATCCAAGAGCACGATTATTCACCAGGGTGAAATAGCGGAAACGCTGTACTACATCTTTATATGCTCTGTGGCAGTGATGATCAAAGACGAAGAGGGTAAAGa                                                                         <  1:1340212/150‑1 (MQ=255)
                                                                               ccGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTG‑AAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGaaa                                                                       <  1:1830745/150‑1 (MQ=255)
                                                                                  aCTCTCGAATGGTTCTTTTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATg                                                                     <  1:828200/150‑1 (MQ=255)
                                                                                     ctcGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATc                                                                  <  2:1639407/150‑1 (MQ=255)
                                                                                     ctcGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATc                                                                  >  1:200339/1‑150 (MQ=255)
                                                                                      tcGAATGGTTCTTGTCTCATTTCCACATTCTTTAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAATCGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGGTGATCAAAGACGAAGAGGGTAAAGAAATGTTcc                                                                 <  1:1082614/150‑1 (MQ=255)
                                                                                      tcGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATcc                                                                 <  1:1223040/150‑1 (MQ=255)
                                                                                      tcGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATcc                                                                 <  1:1040163/150‑1 (MQ=255)
                                                                                         aaTGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCctct                                                              <  1:661908/150‑1 (MQ=255)
                                                                                           tGGTTCTTTTCTAATTGCCAAATTAATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTcc                                                            <  1:1124077/150‑1 (MQ=255)
                                                                                              ttCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCCGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTAt                                                         <  2:1343684/150‑1 (MQ=255)
                                                                                                 ttGTCTCATTGCCACATTCATAAGTACCCAACCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCTAAGACGAAGAGGGTAAAGAAATTATCCTCTCCTATCTg                                                      <  2:1874716/150‑1 (MQ=255)
                                                                                                  tGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGTTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGa                                                     <  1:743747/150‑1 (MQ=255)
                                                                                                  tGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGa                                                     >  2:593475/1‑150 (MQ=255)
                                                                                                  tGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGa                                                     >  2:1238200/1‑150 (MQ=255)
                                                                                                   gTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGaa                                                    <  1:123732/150‑1 (MQ=255)
                                                                                                      tcATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCa                                                 <  2:1122714/150‑1 (MQ=255)
                                                                                                       cATTGCCACATTCATACTTACCCATCCAAGAGCACCCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACCTCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAg                                                <  2:1173262/150‑1 (MQ=255)
                                                                                                       cATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCACTGACGAAGAGGGTAAAGAATTGATCGTCTCCTATCTGattgag                                                >  2:699202/1‑145 (MQ=255)
                                                                                                             cacaTTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGAt                                          >  1:503337/1‑150 (MQ=255)
                                                                                                              acaTTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGAtt                                         >  1:1810203/1‑150 (MQ=255)
                                                                                                               caTTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGAttt                                        <  2:513766/150‑1 (MQ=255)
                                                                                                                 ttCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTa                                      <  1:781409/150‑1 (MQ=255)
                                                                                                                 ttCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTa                                      >  1:620857/1‑150 (MQ=255)
                                                                                                                   cATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTAtt                                    <  2:1750552/150‑1 (MQ=255)
                                                                                                                    aTAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGTTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTg                                   >  2:1196619/1‑150 (MQ=255)
                                                                                                                      aaGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGc                                 <  1:774638/150‑1 (MQ=255)
                                                                                                                        gTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGa                               <  1:1732649/150‑1 (MQ=255)
                                                                                                                        gTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGa                               <  1:1107455/150‑1 (MQ=255)
                                                                                                                         tACCCATCCAAGAGCACGCTTATTCACAAGGGTGAAAAAGCGGAAACGATGAACTACATCTTTAAAGGCTCTGTGGCAGTGGAGATCAAAGACGAAGAGGGTAAAGAAGTGATACTCTCCGTTCGTAAACATCGTGATATTATTGGCGaa                              >  2:250509/1‑150 (MQ=255)
                                                                                                                           cccATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACt                            >  2:475637/1‑150 (MQ=255)
                                                                                                                                        aCGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGaa               <  1:1851824/150‑1 (MQ=255)
                                                                                                                                         ctcttcatCACCAGGGTGAAAAAGCGGAAACGCTGTTCTACATCGTTAAAGGCTCTGTGGCAGTTCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAag              <  2:1680426/143‑1 (MQ=255)
                                                                                                                                         cGCTTTTTCACCCTGGTGAATAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAag              >  2:1448201/1‑150 (MQ=255)
                                                                                                                                         cGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGGGATTTTATTGGCGAACTGGGCCTGTTTGAag              >  1:1450361/1‑150 (MQ=255)
                                                                                                                                         cGCTTATTCACCAGCGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAag              <  2:1540059/150‑1 (MQ=255)
                                                                                                                                         cGCATATTCACCAGTGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGATCTGGGCCTGTTTGAag              <  2:220701/150‑1 (MQ=255)
                                                                                                                                          gCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAaga             >  1:1138877/1‑150 (MQ=255)
                                                                                                                                               ttCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGcc        <  2:1865922/150‑1 (MQ=255)
                                                                                                                                                 cACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAg      >  2:1662588/1‑150 (MQ=255)
                                                                                                                                                 cACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAg      >  2:619591/1‑150 (MQ=255)
                                                                                                                                                 cACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAg      <  1:550630/150‑1 (MQ=255)
                                                                                                                                                 cACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAg      <  2:493021/150‑1 (MQ=255)
                                                                                                                                                   ccAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGa    >  1:879620/1‑150 (MQ=255)
                                                                                                                                                   ccAGAGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGCCCTCTTCAAACAGGCCCAgtt    <  2:5397/150‑3 (MQ=255)
                                                                                                                                                     aGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAAc  <  1:698350/150‑1 (MQ=255)
                                                                                                                                                     aGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGGGCTGATCAAAGACGAAGACGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAAc  <  1:1781230/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAAC  >  NC_000913/3486068‑3486366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑