Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,585,061 C→T 6.2% intergenic (+18/+20) yrhB → / ← ggt stable heat shock chaperone/gamma‑glutamyltranspeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,585,0610CT 6.2% 339.0 / 7.5 113intergenic (+18/+20)yrhB/ggtstable heat shock chaperone/gamma‑glutamyltranspeptidase
Reads supporting (aligned to +/- strand):  ref base C (52/53);  new base T (3/4);  total (56/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

CGCTTAGCTGGTAACGCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGACCTTTTGCT  >  NC_000913/3584912‑3585210
                                                                                                                                                     |                                                                                                                                                     
cgcTTAGCTGGTAACGCACCTTTTATAATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCATccc                                                                                                                                                       <  2:760343/150‑1 (MQ=255)
     aGCTGGTAACGCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGaa                                                                                                                                                  <  1:1282871/150‑1 (MQ=255)
       cTGGTAACGCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTAGCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAaga                                                                                                                                                <  1:481266/150‑1 (MQ=255)
           tAACGCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCGACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCGTGGGAAGAGGGc                                                                                                                                            <  2:171235/150‑1 (MQ=255)
             aCGCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCGTGGGAAGAGGGcta                                                                                                                                          <  1:40749/150‑3 (MQ=255)
             aCGCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCg                                                                                                                                          <  2:198128/150‑1 (MQ=255)
              cGCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGc                                                                                                                                         >  1:1394313/1‑150 (MQ=255)
               gCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCt                                                                                                                                        >  1:753864/1‑150 (MQ=255)
               gCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCt                                                                                                                                        >  2:1562606/1‑150 (MQ=255)
                  ccTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCGTGGGAAGAGGGctattttg                                                                                                                                     <  1:1597089/150‑8 (MQ=255)
                   cTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGTGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAAcc                                                                                                                                    <  1:1032069/150‑1 (MQ=255)
                   cTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAAcc                                                                                                                                    <  1:1005955/150‑1 (MQ=255)
                      ttattaTTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTa                                                                                                                                 <  2:927872/150‑1 (MQ=255)
                       tattatTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAg                                                                                                                                <  1:1543346/150‑1 (MQ=255)
                       tattatTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAg                                                                                                                                <  2:467556/150‑1 (MQ=255)
                              gATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCccg                                                                                                                         >  2:462649/1‑150 (MQ=255)
                                tAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAACACCGCTGGAAGAATATCTACAGGATTAAGATATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGCGGGCCGCTAACCTTAGTACCccgcc                                                                                                                       >  1:315241/1‑150 (MQ=255)
                                tAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCCCGAAGAGGGCCGCTAACCTTAGTACCccgcc                                                                                                                       <  1:755822/150‑1 (MQ=255)
                                  aaGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGt                                                                                                                     <  1:462554/150‑1 (MQ=255)
                                   ataCAGTTGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATATACAGGATTACTAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATATCCCTCATCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGtt                                                                                                                    <  2:421796/148‑1 (MQ=255)
                                       aGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAat                                                                                                                <  2:1621115/150‑1 (MQ=255)
                                          ggtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAatcat                                                                                                             >  2:104988/1‑150 (MQ=255)
                                            tgAAATTGATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAATGGCTACGTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATcc                                                                                                           <  2:133342/150‑1 (MQ=255)
                                            tgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATcc                                                                                                           <  2:931144/150‑1 (MQ=255)
                                               aaTTCATTCTCTGGGAACGGCAAAACCGCTGGATGAATATCTACAGGATTACGAAATAAAAACGGCTACCTTCTGCTTGCCCTGACATAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGAAACCCGCCGTTAAATCATCCaac                                                                                                        >  2:289902/1‑148 (MQ=255)
                                                    attcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcg                                                                                                   >  2:1686477/1‑150 (MQ=255)
                                                     ttcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcgc                                                                                                  >  1:979803/1‑150 (MQ=255)
                                                        tctGTGAACGGCAAAACCGCTGGAAGAATATCTACATTATTACTAAATAAAAAAGTATACCTTCGGCTTGCCCTGACAAAATAGCACTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCggg                                                                                               <  1:285126/150‑1 (MQ=255)
                                                        tctGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCATCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCAGTTAAATCATCCACCGAGCGCggg                                                                                               >  2:424975/1‑150 (MQ=255)
                                                         ctGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGt                                                                                              >  2:747064/1‑150 (MQ=255)
                                                          tgtgAACTGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTc                                                                                             <  1:12002/147‑1 (MQ=255)
                                                            ggAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCgg                                                                                           >  1:1577594/1‑150 (MQ=255)
                                                            ggAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCgg                                                                                           <  1:742291/150‑1 (MQ=255)
                                                              aaCGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGAt                                                                                         <  1:1060666/150‑1 (MQ=255)
                                                                      aaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTa                                                                                 >  1:556532/1‑150 (MQ=255)
                                                                        ccGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACa                                                                               <  2:1265912/150‑1 (MQ=255)
                                                                          gCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGTCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAAc                                                                             <  2:1581185/150‑1 (MQ=255)
                                                                          gCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAAc                                                                             >  1:1820577/1‑150 (MQ=255)
                                                                          gCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAAc                                                                             <  2:603275/150‑1 (MQ=255)
                                                                           cTGTAAGAATATCTACAGGATTACGAATTATAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACt                                                                            <  2:746140/150‑1 (MQ=255)
                                                                           cTGGAAGAATATCTACAGGATTACGATATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACt                                                                            <  2:1095406/150‑1 (MQ=255)
                                                                            tGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTc                                                                           >  1:1554435/1‑150 (MQ=255)
                                                                            tGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCATCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTc                                                                           <  1:285146/150‑1 (MQ=255)
                                                                             ggAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAACTCATCCACCGAGCGCGGGTCGGATGCGCCGTCCCACTcc                                                                          >  1:497192/1‑149 (MQ=255)
                                                                             ggAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACACCTcc                                                                          >  1:504336/1‑149 (MQ=255)
                                                                             ggAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCa                                                                          >  2:1361062/1‑150 (MQ=255)
                                                                                   atatCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTcc                                                                    <  2:1782511/150‑1 (MQ=255)
                                                                                     atCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACACCTCACCGTCCgg                                                                  >  1:1575386/1‑150 (MQ=255)
                                                                                      tCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGc                                                                 <  1:1821023/150‑1 (MQ=255)
                                                                                       cTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGcc                                                                >  1:434627/1‑150 (MQ=255)
                                                                                        tACAGGAGTACGAAATAAAAAAGGCAACCTTCGGCATGCCCTGACAAAATAGCCATCTTCCCACCAAGAGGGCCGCTAACCTTAGTACCCAGCCGATAAATAAACACCCGAGCGCGGGTCGGATGCGCCGTACAACACCCCGTCCggagc                                                               >  1:1507563/1‑147 (MQ=255)
                                                                                            ggATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAAcc                                                           >  2:941560/1‑150 (MQ=255)
                                                                                                 aCGAAATAAAAAAGGCTACCTTCGGCTTCCCCTGACAAACTAGCCCTCATCCCACGAAGAGTGCCGCTAACCTTATTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATTAt                                                      <  1:740123/150‑1 (MQ=255)
                                                                                                  cGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGCAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTCCAACTCACCGTCCGGCCCAACCATAATg                                                     >  2:271915/1‑150 (MQ=255)
                                                                                                  cGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTATATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATaaag                                                     >  2:133622/1‑148 (MQ=255)
                                                                                                  cGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATg                                                     <  2:678642/150‑1 (MQ=255)
                                                                                                  cGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCAACGAAGAGGGCCGCTAACCATAGTACCACGCCGTTAAATCATCCACCCAGCCCGGGTCGGATGCGCCGTCCAACTCACCGTCCGGCCCAACCATAATg                                                     >  1:945179/1‑150 (MQ=255)
                                                                                                  cGAAATAAAAAAGGCTACCTTCGGCTAGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATg                                                     <  2:1205513/150‑1 (MQ=255)
                                                                                                   gAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGc                                                    >  2:385600/1‑150 (MQ=255)
                                                                                                    aaaTAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTTCCCTTAGTACCCCGCCGTTAAATCATCCCCCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGcg                                                   >  2:61505/1‑149 (MQ=255)
                                                                                                     aaTAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCAGCCGTTAAATCATACACCGAGCGCGGGTCGGATGCGCCTTACAACTCACCGTCAGGCCCAACCATAATGAtt                                                  >  2:1592924/1‑150 (MQ=255)
                                                                                                       tAAAAAAGGCTACCTTCGGCTTGCCCTGACTAAATAGCCCTCTTCCCAAGAAGAGGGCCGCTAACCTTAGTACAACGACGTTTAATCATCCACAGAGATCGGGTCGGATGCGCCGTACACCTCACAGTCCGGCACACCCATAATGGttgg                                                >  2:189494/1‑148 (MQ=255)
                                                                                                       tAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGGTAACTCATCCACCGAGCGCGGCTCGGATGCGCCGTCCCACCCACCGTCCGGCCCAACCAGAATGCGTtg                                                >  1:295035/1‑150 (MQ=255)
                                                                                                        aaaaaaGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTtgt                                               >  1:619139/1‑150 (MQ=255)
                                                                                                         aaaaaGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCACCCATAATGCTTtgtg                                              >  1:956165/1‑150 (MQ=255)
                                                                                                         aaaaaGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCATCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTtgtg                                              <  1:1621986/150‑1 (MQ=255)
                                                                                                           aaaGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCGACCATAATGCTTTGTGTa                                            <  2:357851/150‑1 (MQ=255)
                                                                                                            atggCTACCTTCGGCTTGCCCTGACAAAATCGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACAGTCCGGCCCAACCATAATGCTTTGTGTAc                                           <  2:927637/148‑1 (MQ=255)
                                                                                                             aGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACt                                          <  1:1872374/150‑1 (MQ=255)
                                                                                                               gCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCCCCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCCACCAGAATGCTTTGTGTACTGc                                        >  2:1169739/1‑150 (MQ=255)
                                                                                                               gCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGc                                        <  1:869181/150‑1 (MQ=255)
                                                                                                                cTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGcc                                       <  2:1465946/150‑1 (MQ=255)
                                                                                                                cTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGcc                                       >  1:1204467/1‑150 (MQ=255)
                                                                                                                 tACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGccc                                      >  1:453004/1‑150 (MQ=255)
                                                                                                                  aCCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCa                                     >  2:1291687/1‑150 (MQ=255)
                                                                                                                    cTTCGGCTTGCCCTGACAAAATCGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTTAATCATCCACCGCGCGCGGGTCGGATGCGCCGTACAAGTCACCGTCCGGCCCAGCCCTAATGCTTTGTGTACTGCCCATc                                   >  2:1212767/1‑150 (MQ=255)
                                                                                                                         gCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCctc                              <  1:1048309/150‑1 (MQ=255)
                                                                                                                           ttGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCtt                            <  1:1730124/150‑1 (MQ=255)
                                                                                                                             gCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAACTCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCGTTGTGTACTGCCCATCGCCTCTTTc                          >  1:1240883/1‑150 (MQ=255)
                                                                                                                              cccTGACAAAATAGCCCTCTTCCCACGAAGCGGGCCGCGAACCTTAGTACCCCGCCGTTAAATCATCCTCCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCGGGCGCAACCATAATGCTTTGTGTACTGCCCATCGCCGCTTTCa                         >  2:345890/1‑150 (MQ=255)
                                                                                                                              cccTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTACCCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCa                         <  2:841872/150‑1 (MQ=255)
                                                                                                                              cccTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCTGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCa                         <  1:841994/150‑1 (MQ=255)
                                                                                                                              cccTGACAAAATAGACCTCTTCCCACGATGAGGGCCGCTATCCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCa                         <  1:849302/150‑1 (MQ=255)
                                                                                                                               ccTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAg                        <  1:847327/150‑1 (MQ=255)
                                                                                                                                cTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAgc                       >  2:577365/1‑150 (MQ=255)
                                                                                                                                 tGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCCCCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAgcg                      >  2:510425/1‑150 (MQ=255)
                                                                                                                                    cAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCACCCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCa                   >  1:1519607/1‑150 (MQ=255)
                                                                                                                                    cAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGccc                   >  1:1207942/1‑149 (MQ=255)
                                                                                                                                          ggCCCTCTTCGTGGGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCACCCATAATGCGTTGTGTACTGCCCATCGGCTCTTTCAGCGCCACTTTCt             >  1:884350/2‑150 (MQ=255)
                                                                                                                                          ggCCCTCTTCGTGGGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTcg             >  1:623663/2‑149 (MQ=255)
                                                                                                                                          aGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGGGTACTGCCCATCGCCTCTTTCAGCGCCCCTTTcg             >  2:1208474/1‑149 (MQ=255)
                                                                                                                                           gCCCTCTTCGTGGGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTg            >  1:746450/1‑150 (MQ=255)
                                                                                                                                             ccTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCCCCGTCCGGCGCATCCCTACTGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCGACGATCTGAc          >  1:298696/1‑150 (MQ=255)
                                                                                                                                              ctctTCCCACGACGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGAcc         <  2:934256/150‑1 (MQ=255)
                                                                                                                                              ctctTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGAcc         <  2:753864/150‑1 (MQ=255)
                                                                                                                                                  tCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGACCtttt     <  1:1114277/150‑1 (MQ=255)
                                                                                                                                                    ccACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCCCTTTCTGACCTTTTGc   >  1:1722474/1‑150 (MQ=255)
                                                                                                                                                    ccACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGACCTTTTGc   >  1:1488578/1‑150 (MQ=255)
                                                                                                                                                     cACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTACATCATCCACCGAGCGCGGGTCGGATGCGCAGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGCCCtttggat  >  2:295803/1‑146 (MQ=255)
                                                                                                                                                     cACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGACCTTTTGCt  <  2:1577594/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCTTAGCTGGTAACGCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGACCTTTTGCT  >  NC_000913/3584912‑3585210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: