Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,585,062 A→G 5.9% intergenic (+19/+19) yrhB → / ← ggt stable heat shock chaperone/gamma‑glutamyltranspeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,585,0620AG 5.9% 338.4 / 9.6 118intergenic (+19/+19)yrhB/ggtstable heat shock chaperone/gamma‑glutamyltranspeptidase
Reads supporting (aligned to +/- strand):  ref base A (56/54);  new base G (3/4);  total (59/59)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-02

Only 100 of 120 total aligned reads displayed.

CTTAGCTGGTAACGCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGACCTTTTGCTT  >  NC_000913/3584914‑3585211
                                                                                                                                                    |                                                                                                                                                     
cTTAGCTGGTAACGCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGATGAATATCAACAGGATTACGAAATAAAAAAGTCTACCTTCGGCTTGACCTTACAAAATAGCCCTCTTCacac                                                                                                                                                      >  1:218976/1‑150 (MQ=255)
cTTAGCTGGTAACGCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCAc                                                                                                                                                      <  2:376292/150‑1 (MQ=255)
  tAGCTGGTAACGCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAAAATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGa                                                                                                                                                    >  2:1114277/1‑150 (MQ=255)
   aGCTGGTAACGCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGaa                                                                                                                                                   <  1:1282871/150‑1 (MQ=255)
    gCTGGTAACGCACATTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAag                                                                                                                                                  <  1:890302/150‑1 (MQ=255)
         tAACGCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCGACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCGTGGGAAGAGGGc                                                                                                                                             <  2:171235/150‑1 (MQ=255)
           aCGCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCGTGGGAAGAGGGcta                                                                                                                                           <  1:40749/150‑3 (MQ=255)
            cGCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGc                                                                                                                                          >  1:1394313/1‑150 (MQ=255)
             gCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCt                                                                                                                                         >  2:1562606/1‑150 (MQ=255)
             gCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCt                                                                                                                                         >  1:753864/1‑150 (MQ=255)
                ccTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCGTGGGAAGAGGGctattttg                                                                                                                                      <  1:1597089/150‑8 (MQ=255)
                 cTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGTGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAAcc                                                                                                                                     <  1:1032069/150‑1 (MQ=255)
                 cTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAAcc                                                                                                                                     <  1:1005955/150‑1 (MQ=255)
                    ttattaTTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTa                                                                                                                                  <  2:927872/150‑1 (MQ=255)
                     tattatTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAg                                                                                                                                 <  1:1543346/150‑1 (MQ=255)
                            gATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCccg                                                                                                                          >  2:462649/1‑150 (MQ=255)
                            gATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCccg                                                                                                                          <  1:338406/150‑1 (MQ=255)
                               aaaGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCccgccg                                                                                                                       <  2:488313/150‑1 (MQ=255)
                                aaGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGt                                                                                                                      <  1:462554/150‑1 (MQ=255)
                                 ataCAGTTGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATATACAGGATTACTAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATATCCCTCATCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGtt                                                                                                                     <  2:421796/148‑1 (MQ=255)
                                        ggtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAatcat                                                                                                              >  2:104988/1‑150 (MQ=255)
                                        ggtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAatcat                                                                                                              >  2:1664945/1‑150 (MQ=255)
                                          tgAAATTGATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAATGGCTACGTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATcc                                                                                                            <  2:133342/150‑1 (MQ=255)
                                          tgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATcc                                                                                                            <  2:931144/150‑1 (MQ=255)
                                             aaTTCATTCTCTGGGAACGGCAAAACCGCTGGATGAATATCTACAGGATTACGAAATAAAAACGGCTACCTTCTGCTTGCCCTGACATAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGAAACCCGCCGTTAAATCATCCaac                                                                                                         >  2:289902/1‑148 (MQ=255)
                                                  attcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcg                                                                                                    >  2:1686477/1‑150 (MQ=255)
                                                   ttcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcgc                                                                                                   >  1:979803/1‑150 (MQ=255)
                                                      tctGTGAACGGCAAAACCGCTGGAAGAATATCTACATTATTACTAAATAAAAAAGTATACCTTCGGCTTGCCCTGACAAAATAGCACTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCggg                                                                                                <  1:285126/150‑1 (MQ=255)
                                                      tctGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCATCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCAGTTAAATCATCCACCGAGCGCggg                                                                                                >  2:424975/1‑150 (MQ=255)
                                                       ctGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGt                                                                                               >  2:747064/1‑150 (MQ=255)
                                                        tgtgAACTGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTc                                                                                              <  1:12002/147‑1 (MQ=255)
                                                          ggAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCgg                                                                                            >  1:1577594/1‑150 (MQ=255)
                                                          ggAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCgg                                                                                            <  1:742291/150‑1 (MQ=255)
                                                            aaCGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGAt                                                                                          <  1:1060666/150‑1 (MQ=255)
                                                               ggCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAACAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATgcg                                                                                       <  1:491233/150‑1 (MQ=255)
                                                                    aaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTa                                                                                  >  1:556532/1‑150 (MQ=255)
                                                                        gCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGTCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAAc                                                                              <  2:1581185/150‑1 (MQ=255)
                                                                        gCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAAc                                                                              >  1:1820577/1‑150 (MQ=255)
                                                                        gCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAAc                                                                              <  2:603275/150‑1 (MQ=255)
                                                                         cTGTAAGAATATCTACAGGATTACGAATTATAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACt                                                                             <  2:746140/150‑1 (MQ=255)
                                                                         cTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACt                                                                             >  2:728445/1‑150 (MQ=255)
                                                                          tGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTc                                                                            >  1:1554435/1‑150 (MQ=255)
                                                                          tGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCATCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTc                                                                            <  1:285146/150‑1 (MQ=255)
                                                                           ggAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAACTCATCCACCGAGCGCGGGTCGGATGCGCCGTCCCACTcc                                                                           >  1:497192/1‑149 (MQ=255)
                                                                           ggAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACACCTcc                                                                           >  1:504336/1‑149 (MQ=255)
                                                                           ggAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCa                                                                           >  2:1361062/1‑150 (MQ=255)
                                                                                 atatCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTcc                                                                     <  2:1782511/150‑1 (MQ=255)
                                                                                   atCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACACCTCACCGTCCgg                                                                   >  1:1575386/1‑150 (MQ=255)
                                                                                    tCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGc                                                                  <  1:1821023/150‑1 (MQ=255)
                                                                                     cTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGcc                                                                 >  1:434627/1‑150 (MQ=255)
                                                                                      tACAGGAGTACGAAATAAAAAAGGCAACCTTCGGCATGCCCTGACAAAATAGCCATCTTCCCACCAAGAGGGCCGCTAACCTTAGTACCCAGCCGATAAATAAACACCCGAGCGCGGGTCGGATGCGCCGTACAACACCCCGTCCggagc                                                                >  1:1507563/1‑147 (MQ=255)
                                                                                          ggATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAAcc                                                            >  2:941560/1‑150 (MQ=255)
                                                                                              tACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCACCCATaa                                                        >  2:1286372/1‑150 (MQ=255)
                                                                                               aCGAAATAAAAAAGGCTACCTTCGGCTTCCCCTGACAAACTAGCCCTCATCCCACGAAGAGTGCCGCTAACCTTATTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATTAt                                                       <  1:740123/150‑1 (MQ=255)
                                                                                                cGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGCAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTCCAACTCACCGTCCGGCCCAACCATAATg                                                      >  2:271915/1‑150 (MQ=255)
                                                                                                cGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTATATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATaaag                                                      >  2:133622/1‑148 (MQ=255)
                                                                                                cGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCAACGAAGAGGGCCGCTAACCATAGTACCACGCCGTTAAATCATCCACCCAGCCCGGGTCGGATGCGCCGTCCAACTCACCGTCCGGCCCAACCATAATg                                                      >  1:945179/1‑150 (MQ=255)
                                                                                                cGAAATAAAAAAGGCTACCTTCGGCTAGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATg                                                      <  2:1205513/150‑1 (MQ=255)
                                                                                                 gAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGc                                                     >  2:385600/1‑150 (MQ=255)
                                                                                                  aaaTAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTTCCCTTAGTACCCCGCCGTTAAATCATCCCCCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGcg                                                    >  2:61505/1‑149 (MQ=255)
                                                                                                   aaTAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCAGCCGTTAAATCATACACCGAGCGCGGGTCGGATGCGCCTTACAACTCACCGTCAGGCCCAACCATAATGAtt                                                   >  2:1592924/1‑150 (MQ=255)
                                                                                                     tAAAAAAGGCTACCTTCGGCTTGCCCTGACTAAATAGCCCTCTTCCCAAGAAGAGGGCCGCTAACCTTAGTACAACGACGTTTAATCATCCACAGAGATCGGGTCGGATGCGCCGTACACCTCACAGTCCGGCACACCCATAATGGttgg                                                 >  2:189494/1‑148 (MQ=255)
                                                                                                     tAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGGTAACTCATCCACCGAGCGCGGCTCGGATGCGCCGTCCCACCCACCGTCCGGCCCAACCAGAATGCGTtg                                                 >  1:295035/1‑150 (MQ=255)
                                                                                                      aaaaaaGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTtgt                                                >  1:619139/1‑150 (MQ=255)
                                                                                                       aaaaaGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCACCCATAATGCTTtgtg                                               >  1:956165/1‑150 (MQ=255)
                                                                                                          atggCTACCTTCGGCTTGCCCTGACAAAATCGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACAGTCCGGCCCAACCATAATGCTTTGTGTAc                                            <  2:927637/148‑1 (MQ=255)
                                                                                                           aGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACt                                           <  2:21065/150‑1 (MQ=255)
                                                                                                             gCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCCCCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCCACCAGAATGCTTTGTGTACTGc                                         >  2:1169739/1‑150 (MQ=255)
                                                                                                             gCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGc                                         <  1:869181/150‑1 (MQ=255)
                                                                                                              cTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGcc                                        >  1:1204467/1‑150 (MQ=255)
                                                                                                               tACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGccc                                       >  1:453004/1‑150 (MQ=255)
                                                                                                                aCCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCa                                      >  2:1291687/1‑150 (MQ=255)
                                                                                                                  cTTCGGCTTGCCCTGACAAAATCGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTTAATCATCCACCGCGCGCGGGTCGGATGCGCCGTACAAGTCACCGTCCGGCCCAGCCCTAATGCTTTGTGTACTGCCCATc                                    >  2:1212767/1‑150 (MQ=255)
                                                                                                                       gCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCctc                               <  1:1048309/150‑1 (MQ=255)
                                                                                                                         ttGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCtt                             <  1:1730124/150‑1 (MQ=255)
                                                                                                                           gCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAACTCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCGTTGTGTACTGCCCATCGCCTCTTTc                           >  1:1240883/1‑150 (MQ=255)
                                                                                                                            cccTGACAAAATAGCCCTCTTCCCACGAAGCGGGCCGCGAACCTTAGTACCCCGCCGTTAAATCATCCTCCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCGGGCGCAACCATAATGCTTTGTGTACTGCCCATCGCCGCTTTCa                          >  2:345890/1‑150 (MQ=255)
                                                                                                                            cccTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTACCCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCa                          <  2:841872/150‑1 (MQ=255)
                                                                                                                            cccTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCTGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCa                          <  1:841994/150‑1 (MQ=255)
                                                                                                                            cccTGACAAAATAGACCTCTTCCCACGATGAGGGCCGCTATCCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCa                          <  1:849302/150‑1 (MQ=255)
                                                                                                                             ccTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAg                         <  1:847327/150‑1 (MQ=255)
                                                                                                                             ccTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAg                         >  1:820007/1‑150 (MQ=255)
                                                                                                                              cTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAgc                        >  2:577365/1‑150 (MQ=255)
                                                                                                                               tGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCCCCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAgcg                       >  2:510425/1‑150 (MQ=255)
                                                                                                                                  cAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCACCCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCa                    >  1:1519607/1‑150 (MQ=255)
                                                                                                                                  cAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGccc                    >  1:1207942/1‑149 (MQ=255)
                                                                                                                                       tAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTc               <  2:1554435/150‑1 (MQ=255)
                                                                                                                                        aGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGGGTACTGCCCATCGCCTCTTTCAGCGCCCCTTTcg              >  2:1208474/1‑149 (MQ=255)
                                                                                                                                         gCCCTCTTCGTGGGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTg             >  1:746450/1‑150 (MQ=255)
                                                                                                                                           ccTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCCCCGTCCGGCGCATCCCTACTGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCGACGATCTGAc           >  1:298696/1‑150 (MQ=255)
                                                                                                                                            ctctTCCCACGACGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGAcc          <  2:934256/150‑1 (MQ=255)
                                                                                                                                            ctctTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGAcc          <  2:753864/150‑1 (MQ=255)
                                                                                                                                             tctTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGACCt         <  1:728445/150‑1 (MQ=255)
                                                                                                                                                tCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGACCtttt      <  1:1114277/150‑1 (MQ=255)
                                                                                                                                                 cccACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGACCTTTTg     <  1:1656922/150‑1 (MQ=255)
                                                                                                                                                  ccACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCCCTTTCTGACCTTTTGc    >  1:1722474/1‑150 (MQ=255)
                                                                                                                                                  ccACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGACCTTTTGc    >  1:1488578/1‑150 (MQ=255)
                                                                                                                                                   cACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTACATCATCCACCGAGCGCGGGTCGGATGCGCAGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGCCCtttggat   >  2:295803/1‑146 (MQ=255)
                                                                                                                                                   cACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGACCTTTTGCt   <  2:1577594/150‑1 (MQ=255)
                                                                                                                                                    aCGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGACCTTTTGCtt  <  1:1562606/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTTAGCTGGTAACGCACCTTTTATTATTGATAAAGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCCCATCGCCTCTTTCAGCGCCACTTTCTGACCTTTTGCTT  >  NC_000913/3584914‑3585211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: