Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,639,217 C→A 5.8% intergenic (+76/+168) pitA → / ← uspB phosphate transporter, low‑affinity; tellurite importer/universal stress (ethanol tolerance) protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,639,2170CA 5.8% 350.9 / 7.9 125intergenic (+76/+168)pitA/uspBphosphate transporter, low‑affinity; tellurite importer/universal stress (ethanol tolerance) protein B
Reads supporting (aligned to +/- strand):  ref base C (58/58);  new base A (4/4);  total (63/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 129 total aligned reads displayed.

GGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCG  >  NC_000913/3639071‑3639366
                                                                                                                                                  |                                                                                                                                                     
gggTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATgcggc                                                                                                                                                    >  1:1096663/1‑150 (MQ=255)
  gtgaTTACCCTTCCGGCTGAGGTACTGCTTTCCGCCGGGCTGCACTGGCTCTCCTTGCAGTTCCCGTAATCGTACGCACCAAACCGAGCGGGTCCGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGt                                                                                                                                                  <  2:1648731/150‑1 (MQ=255)
   tgtTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTg                                                                                                                                                 <  2:1819385/150‑1 (MQ=255)
    gtTTACCCTTCCGCCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGCGCGGGTCAGCTGGCCCGCTTCAGATTGGGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGa                                                                                                                                                <  2:369519/150‑1 (MQ=255)
      ttACCCTTGCGGCTGCGGTACTGCTTTCGGGCGGGCTGTTCTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTGCATGCCGGATGCGGCGTGAAc                                                                                                                                              <  2:1323506/150‑1 (MQ=255)
          ccTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGAACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCt                                                                                                                                          >  2:659759/1‑150 (MQ=255)
           cTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCACGCCGCATCCGGCATGAAcaaagc                                                                                                                                         <  1:259804/150‑6 (MQ=255)
           cTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGACCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCtt                                                                                                                                         >  1:1314970/1‑150 (MQ=255)
                ggCTGCGGTACTGCTTTCCGGGGGGCTGTACTGGCTCTCCTTGCCGTTCCGGTAATCGTACGCACCAAAACTAGGGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCg                                                                                                                                    <  2:1661107/150‑1 (MQ=38)
                  cTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGCGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                  <  1:1185881/150‑1 (MQ=255)
                   tGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                 <  2:505802/150‑1 (MQ=255)
                    gCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                                                                                                                <  2:1067192/150‑1 (MQ=255)
                     cGGTACTGCTTTCCGGCGGGTTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGcatg                                                                                                                               <  1:1812066/150‑4 (MQ=38)
                      ggTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc                                                                                                                              >  2:1093796/1‑150 (MQ=255)
                      ggTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGcatga                                                                                                                              <  1:1042528/150‑5 (MQ=38)
                           tGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAACCGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaaaaa                                                                                                                         <  1:749856/150‑1 (MQ=255)
                           tGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGAGTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaaata                                                                                                                         >  2:1448418/1‑148 (MQ=255)
                            gCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAAc                                                                                                                        >  2:98001/1‑150 (MQ=255)
                             cTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACa                                                                                                                       <  2:186992/150‑1 (MQ=255)
                              tttCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACAt                                                                                                                      >  1:314088/1‑150 (MQ=255)
                                 ccGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCa                                                                                                                   <  2:461756/150‑1 (MQ=255)
                                  cggcggGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCaa                                                                                                                  <  1:419845/150‑1 (MQ=255)
                                  cggcggGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCaa                                                                                                                  >  2:1147179/1‑150 (MQ=255)
                                  cggcggGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCaa                                                                                                                  >  1:1150919/1‑150 (MQ=255)
                                      gggCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAACACGAGCGGGTCAGCTGGCCCGCTTCAGATTGAGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTc                                                                                                              <  2:332121/150‑1 (MQ=255)
                                       ggCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCa                                                                                                             <  2:710217/150‑1 (MQ=255)
                                          tGTACTGGCTCTCCTTGCAGCTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATa                                                                                                          <  2:35006/150‑1 (MQ=255)
                                            tACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGa                                                                                                        >  1:894930/1‑150 (MQ=255)
                                              cTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGAtt                                                                                                      >  2:537919/1‑150 (MQ=255)
                                                ggCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGc                                                                                                    <  2:597696/150‑1 (MQ=255)
                                                  ctctCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCag                                                                                                  >  1:525311/1‑150 (MQ=255)
                                                   tctcCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCaga                                                                                                 >  1:1319365/1‑150 (MQ=255)
                                                   tctcCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCaga                                                                                                 <  1:832395/150‑1 (MQ=255)
                                                     tcCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGAGCGCTTTGTTCATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTAAATAGATTGCAgaga                                                                                               >  1:804741/1‑150 (MQ=255)
                                                         tGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTAt                                                                                           >  2:139816/1‑150 (MQ=255)
                                                         cgCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGAGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCATATTCAATAGATTGCAGAGATTAt                                                                                           >  1:697319/2‑150 (MQ=255)
                                                           cAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGt                                                                                         <  1:869174/150‑1 (MQ=255)
                                                           cAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGt                                                                                         <  1:400569/150‑1 (MQ=255)
                                                            aGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTa                                                                                        >  1:1128880/1‑150 (MQ=255)
                                                                ccTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGcc                                                                                    <  2:1096663/150‑1 (MQ=255)
                                                                 cTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCt                                                                                   >  2:527452/1‑150 (MQ=255)
                                                                 cTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCt                                                                                   >  1:638897/1‑150 (MQ=255)
                                                                  tGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTCCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTg                                                                                  <  2:948571/150‑1 (MQ=255)
                                                                      aTCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATaa                                                                              >  2:482330/1‑150 (MQ=255)
                                                                       tCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAg                                                                             <  2:660639/150‑1 (MQ=255)
                                                                        catACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGc                                                                            <  2:412101/148‑1 (MQ=255)
                                                                         gTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCg                                                                           <  2:1616453/150‑1 (MQ=255)
                                                                           aCGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTa                                                                         >  1:741639/1‑150 (MQ=255)
                                                                            cGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAg                                                                        >  1:186447/1‑150 (MQ=255)
                                                                              cACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAgcg                                                                      >  2:761296/1‑150 (MQ=255)
                                                                              cACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAgcg                                                                      >  2:1680074/1‑150 (MQ=255)
                                                                              cACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAgcg                                                                      >  2:1177619/1‑150 (MQ=255)
                                                                               aCCAAATCGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAgcgc                                                                     >  2:304762/1‑150 (MQ=255)
                                                                               aCCAAATCGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAgcgc                                                                     >  2:304761/1‑150 (MQ=255)
                                                                               aCCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAgcgc                                                                     >  2:1031662/1‑150 (MQ=255)
                                                                               aCCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAgcgc                                                                     >  1:434039/1‑150 (MQ=255)
                                                                                 aatctgaaGCGGGCCAGCTGACCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCAt                                                                   >  1:1861052/8‑150 (MQ=255)
                                                                                   tctgaaGCGGGCCAGCTGACCCGCTTCAGATTGTGACATAGAGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATACAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGAAGCGCATCa                                                                 >  2:1361483/6‑150 (MQ=21)
                                                                                   aaaCGAGCGGGGCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCa                                                                 <  1:1471080/150‑1 (MQ=255)
                                                                                      gaaGCGGGCCAGCTGACCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGc                                                              >  1:1310836/3‑150 (MQ=255)
                                                                                       gAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                             >  2:223644/1‑150 (MQ=255)
                                                                                            ggTCCGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAACTCATGCAAATTCAATAGATTGCAGAGATTATGTAGGCGTGATAAGCGTAGCGCATCAGGCAAtttt                                                        >  1:1310052/1‑150 (MQ=255)
                                                                                              tCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGc                                                      <  2:1819172/150‑1 (MQ=255)
                                                                                              tCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGc                                                      <  2:1760285/150‑1 (MQ=255)
                                                                                                 gCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGtt                                                   >  2:677498/1‑150 (MQ=255)
                                                                                                   tGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTg                                                 <  2:485442/150‑1 (MQ=255)
                                                                                                   tGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTg                                                 <  1:272273/150‑1 (MQ=255)
                                                                                                      cccGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTaa                                              >  1:589583/1‑150 (MQ=255)
                                                                                                       ccGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAAt                                             <  2:1628553/150‑1 (MQ=255)
                                                                                                            tCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGtc                                        <  2:464192/150‑1 (MQ=255)
                                                                                                                 ttGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAg                                   <  2:1231724/150‑1 (MQ=255)
                                                                                                                   gtgACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCg                                 <  2:1150919/150‑1 (MQ=255)
                                                                                                                    tgACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCgg                                <  1:1767255/150‑1 (MQ=255)
                                                                                                                           gTGCGCTTTGTTCATGCCGGATGTGGCGTGAACGCCTTATCCGGCCTACAAAACCATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGtt                         >  2:273070/1‑150 (MQ=255)
                                                                                                                            tGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGttt                        >  2:1683000/1‑150 (MQ=255)
                                                                                                                            tGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGttt                        >  2:1759680/1‑150 (MQ=255)
                                                                                                                              cgcTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTAc                      <  1:659759/150‑1 (MQ=255)
                                                                                                                                cTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTg                    >  1:330293/1‑150 (MQ=255)
                                                                                                                                cTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGAGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTg                    >  2:972675/1‑150 (MQ=255)
                                                                                                                                  ttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGc                  <  1:1183958/150‑1 (MQ=255)
                                                                                                                                    tttCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGTGTCAGTTTACTGGccc                <  1:293483/149‑1 (MQ=255)
                                                                                                                                    gTTCATGCCGGATGCGGCGTGAACGCCTTAGCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGGATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGccc                <  1:363126/150‑1 (MQ=255)
                                                                                                                                     ttCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTTTGTAGGCCTGATTAGCGTAGCGCAGCAGGCTATTTTGGGTTTGTTATCAGTCTCGAGCGGGTCTGGTTTCTGGccgg               >  2:1746387/1‑148 (MQ=18)
                                                                                                                                     atCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAATAACATGCAAATTCAATAGAATGCAGAGAGTTTGTAGGCCTCATAAGCGTAGCGCATCAGGCAATTTTGCGTAAGTACTCAGACTCGAGCGGGTCAGTGTACTCGCCCg               >  1:41203/2‑150 (MQ=18)
                                                                                                                                        aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCtt            >  2:403909/3‑150 (MQ=21)
                                                                                                                                        aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGTGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCtt            >  1:1592820/1‑150 (MQ=255)
                                                                                                                                         tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATCGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCttt           <  2:793568/150‑1 (MQ=255)
                                                                                                                                         tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCttt           >  1:813807/1‑150 (MQ=255)
                                                                                                                                          gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCtttt          >  1:186582/1‑150 (MQ=255)
                                                                                                                                          gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCtttt          <  2:1512615/150‑1 (MQ=255)
                                                                                                                                           ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCTTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCttttt         >  2:1592268/1‑150 (MQ=255)
                                                                                                                                            cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTTCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCtttttt        <  1:871988/150‑1 (MQ=255)
                                                                                                                                            cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCtttttt        <  2:590782/150‑1 (MQ=255)
                                                                                                                                            cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCtttttt        <  2:1814368/150‑1 (MQ=255)
                                                                                                                                             ggATGCGACGGGAACGCCTTATCCGGCCTACAAAAACATGCAATTTCATTAGATTGCAGAGATTATTTCGGCCTTATATTCGTAGAGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGCCAGTAAACTGACCCGCtcgagac       <  2:1433022/150‑7 (MQ=18)
                                                                                                                                               ctGCGGCGTGAACGCCTTATCCGGCCTACAAAAACCTGCAAATTCAATAGATTGCAGAGATAATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGGTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTAt     <  2:251495/149‑1 (MQ=255)
                                                                                                                                                tGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGTTTGCAGAGATTCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATg    <  1:1205166/150‑1 (MQ=255)
                                                                                                                                                tGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATg    <  2:1826228/150‑1 (MQ=255)
                                                                                                                                                 gcggcgTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGc   <  2:741639/150‑1 (MQ=255)
                                                                                                                                                  cggcgTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCg  >  1:926771/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCG  >  NC_000913/3639071‑3639366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: