BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	423,989	A→C	5.2%	N491T (AAC→ACC)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	423,989	0	A	C	5.2%	308.1 / 4.9	108	N491T (AAC→ACC)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand): ref base A (58/44); new base C (4/2); total (62/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 110 total aligned reads displayed.

TAATCAGCTCGACAGCCACGATACTGCGCGATTTAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGA  >  NC_000913/423840‑424133

tAATCAGCTCGACAGCCACGATACTGCGCGATTTAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCaaaaa                                                                                                                                                  <  2:459868/150‑1 (MQ=255)

  aTCAGCTCGACAGCCACGATACTGCGCGATTTAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACg                                                                                                                                                >  1:1851985/1‑150 (MQ=255)

   tCAGCTCGACAGCCACGATACTGCGCGATTTAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGa                                                                                                                                               >  2:793006/1‑150 (MQ=255)

    cAGCTCGACAGCCACGATACTGCGCGATTTAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGAt                                                                                                                                              <  1:1397019/150‑1 (MQ=255)

     aGCTCGACAGCCACGATACTGCGCGATTTAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATc                                                                                                                                             <  1:1217488/150‑1 (MQ=255)

     aGCTCGACAGCCACGATACTGCGCGATTTAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATc                                                                                                                                             <  1:1380285/150‑1 (MQ=255)

      gCTCGACAGCCACGATACTGCGCGATTTAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATcc                                                                                                                                            >  1:379441/1‑150 (MQ=255)

         cGACAGCCACGATACTGCGCGATTTAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGtt                                                                                                                                         >  1:1476420/1‑150 (MQ=255)

             aGCCACGATACTGCGCGATTTAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGc                                                                                                                                     <  2:1671533/150‑1 (MQ=255)

               ccACGATACTGCGCGATTTAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCg                                                                                                                                   >  1:394771/1‑150 (MQ=255)

                 ccGATACTGCGCGATTTAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAACGGATCGTTTTTGccatc                                                                                                                                 <  1:965036/149‑4 (MQ=255)

                    aTACTGCGCGATTTAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAAc                                                                                                                              >  2:577366/1‑150 (MQ=255)

                     tACTTCGCGATGTAACCCGCTGCTCGGGCGGGATATTGCGCGCCTGCCGCGGGCGGGGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTCCGGTGAGGAAGTAGGACTGGCTGGCAAAAACGATCCGTTTTGCCGTAAAcc                                                                                                                             <  2:215042/150‑1 (MQ=255)

                       cTGCGCGATTTAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGt                                                                                                                           >  1:393390/1‑150 (MQ=255)

                        tGCGCGATTTAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGtt                                                                                                                          >  1:691808/1‑150 (MQ=255)

                          cgcgATTTAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGATCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTcc                                                                                                                        >  2:245363/1‑150 (MQ=255)

                            ctaTTTAAAACGCTGCTCGGTGGGGATCTTGCGCGCCTGACTATGTCGGTTGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCTTTTATTACGGTGCTGAAGTAGGACTGGATGGCAAAACCGATCCGTTTTGACGTAAACCGTTcccc                                                                                                                      <  1:171394/148‑1 (MQ=255)

                             gATTTAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCt                                                                                                                     >  1:212862/1‑150 (MQ=255)

                               tttAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTgg                                                                                                                   >  1:545262/1‑150 (MQ=255)

                                 tAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCa                                                                                                                 >  2:1716675/1‑150 (MQ=255)

                                 tAAAACGCTGCTCGGTCGGGATATTGCGCGCATGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAAAGATCCGTTTTGCCGTAAACCGTTCCCCTGGCa                                                                                                                 >  1:1462203/1‑150 (MQ=255)

                                   aaaCGCTGCTCGGTCGGGAAATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGGTCACCGGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTATGCCTTAAACCTTTCCCCTGGGAgg                                                                                                               >  2:1775111/1‑150 (MQ=255)

                                     aCGCAGCGCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGGGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTg                                                                                                             <  1:260203/150‑1 (MQ=255)

                                      cGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGGGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTgg                                                                                                            <  1:138196/150‑1 (MQ=255)

                                       gctgctCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGa                                                                                                           <  1:1078000/150‑1 (MQ=255)

                                           gtcggtcggGATATTTCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAg                                                                                                       <  1:639147/149‑1 (MQ=255)

                                              ggtcggGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAg                                                                                                    <  1:1279315/150‑1 (MQ=255)

                                              ggtcggGATATGGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGATCACCTGGCCGGGTGTACCGTGCATTTATTCCGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAg                                                                                                    <  2:366625/150‑1 (MQ=255)

                                               gtcggGCTCTTGCGCGCCTGCCGCGGGCGGTGGTCAGTCTGTTGACCGGGCCTGGGGTACCGTGCATTTAATACGGAGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAgg                                                                                                   <  1:298945/150‑1 (MQ=255)

                                               ggcggGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAgg                                                                                                   <  2:1854714/148‑1 (MQ=255)

                                                   ggATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTAGTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACTGTTCCCCTGGCAGGTGGAAAAGCAGGATAc                                                                                               >  1:979834/1‑150 (MQ=255)

                                                      tatTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGc                                                                                            <  1:558803/150‑1 (MQ=255)

                                                       atTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCg                                                                                           <  2:1070965/150‑1 (MQ=255)

                                                       atTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCg                                                                                           >  1:1077889/1‑150 (MQ=255)

                                                           cgcgcCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGGGTACCTTTCATTTATTACGGTGATGAAGTAGGACTGCATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTAt                                                                                       <  2:312574/150‑1 (MQ=255)

                                                              gcCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTcg                                                                                    <  1:1104616/150‑1 (MQ=255)

                                                               ccTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCATAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTcgc                                                                                   <  2:792161/150‑1 (MQ=255)

                                                                 tGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCgcgc                                                                                 <  1:38119/150‑1 (MQ=255)

                                                                 tGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCACTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCgcgc                                                                                 >  1:242631/1‑150 (MQ=255)

                                                                  gCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCt                                                                                <  2:1711955/150‑1 (MQ=255)

                                                                      cgggCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGCAGTAGGACTGGATGCCAAAAACGATCCGGTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTAc                                                                            <  2:1316723/148‑1 (MQ=255)

                                                                      cTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTAc                                                                            >  1:228140/1‑150 (MQ=255)

                                                                       gggCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTAcc                                                                           <  2:1208154/149‑1 (MQ=255)

                                                                       gggCGGGGGGCTGGCTGTTCACCTGGCCTGGTGAACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGATCCCCAGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTAcc                                                                           <  1:278742/149‑1 (MQ=255)

                                                                        ggCGGTGGTCTGGCTGTTCACCTGGACTGGTGAACCGTGCATTTATTACGGTGATGAAGTAGGACAGGATGGCAAAGACGATCCGTTTTGCCGTAATCCGATCCCCTGGCAGGTGGAAACGCAGGATACGTCGTTATTAGCGCTGTACCa                                                                          >  1:1372956/1‑150 (MQ=255)

                                                                             gggtCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGaa                                                                     <  2:1721510/149‑1 (MQ=255)

                                                                                tCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTAATACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTTCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGTGCTCTACAAGCGAATGa                                                                  >  1:1527614/1‑150 (MQ=255)

                                                                                 cTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGAt                                                                 >  2:1758432/1‑150 (MQ=255)

                                                                                 cTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGAt                                                                 <  2:1058624/150‑1 (MQ=255)

                                                                                 cTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAAAGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGAt                                                                 >  2:98045/1‑150 (MQ=255)

                                                                                  tGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGAtt                                                                <  2:1476420/150‑1 (MQ=255)

                                                                                      tGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTgcgc                                                            >  1:1271237/1‑150 (MQ=255)

                                                                                       gTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCt                                                           >  1:453098/1‑150 (MQ=255)

                                                                                        ttCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTg                                                          >  1:1254343/1‑150 (MQ=255)

                                                                                          cACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGTAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCg                                                        >  2:813907/1‑150 (MQ=255)

                                                                                           aCCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAATCCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGt                                                       >  1:1201927/1‑150 (MQ=255)

                                                                                            cctggcctggTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTa                                                      <  2:1084913/150‑1 (MQ=255)

                                                                                               ggccgggTGTACCGTGCAGTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGa                                                   <  2:379441/150‑1 (MQ=255)

                                                                                                  ctggTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCG‑TTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAg                                               >  1:20153/1‑150 (MQ=255)

                                                                                                     gtgtACCGTGCAATTATTACGGTGATGAAGTCGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTc                                             <  2:1511253/150‑1 (MQ=255)

                                                                                                      tgtACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCa                                            >  1:565110/1‑150 (MQ=255)

                                                                                                      tgtACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCa                                            <  1:577366/150‑1 (MQ=255)

                                                                                                            gTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCt                                      >  2:334456/1‑150 (MQ=255)

                                                                                                             ggCATTTATTCCGCTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCCGGGGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTa                                     <  2:73751/149‑1 (MQ=255)

                                                                                                              gCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTAc                                    <  1:1251441/150‑1 (MQ=255)

                                                                                                                aTTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAATCCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGt                                  >  2:833652/1‑150 (MQ=255)

                                                                                                                 tttATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTc                                 >  1:1042628/1‑150 (MQ=255)

                                                                                                                   tattaCGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAAGGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCAt                               >  2:134595/1‑150 (MQ=255)

                                                                                                                   tattaCGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCACTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGCATGATTTCGCTGCGTAAGAAAAGTCAGGCGCTACGTCAt                               >  1:906820/1‑150 (MQ=255)

                                                                                                                        cGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATggcgg                          >  2:1028056/1‑150 (MQ=255)

                                                                                                                         ggggatgaAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGGAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGGCATggcggc                         <  2:734045/147‑1 (MQ=255)

                                                                                                                         ggTGATGAAGTCGGACTGGATGGCAAAAACGATCCGTTTTGCCGGAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATggcggc                         <  2:177784/150‑1 (MQ=255)

                                                                                                                         ggTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATggcggc                         >  1:1456122/1‑150 (MQ=255)

                                                                                                                         ggTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATAGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATggcggc                         >  1:44575/1‑150 (MQ=255)

                                                                                                                           tgatgaAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTg                       <  2:479211/150‑1 (MQ=255)

                                                                                                                           tgatgaAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTg                       <  2:1851985/150‑1 (MQ=255)

                                                                                                                             atgaAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTc                     >  2:599718/1‑150 (MQ=255)

                                                                                                                              tgaAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCa                    >  1:724812/1‑150 (MQ=255)

                                                                                                                              tgaAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCa                    >  1:1560883/1‑150 (MQ=255)

                                                                                                                                aaGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAgg                  >  1:1803590/1‑150 (MQ=255)

                                                                                                                                 aGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCGTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAggg                 >  2:907317/1‑149 (MQ=255)

                                                                                                                                 aGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGt                 >  1:1852094/1‑150 (MQ=255)

                                                                                                                                 aGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGt                 >  1:907808/1‑150 (MQ=255)

                                                                                                                                     ggACTGGATTGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTg             <  2:1703373/150‑1 (MQ=255)

                                                                                                                                     ggACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGGGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTATGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTg             >  2:965744/1‑150 (MQ=255)

                                                                                                                                       aCTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTa           >  2:119516/1‑150 (MQ=255)

                                                                                                                                        gtttacggCAAAACGGAT‑CGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTAGCGCTGTACCAGAGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATg         >  2:1278066/7‑150 (MQ=255)

                                                                                                                                           gATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCg       <  2:630465/150‑1 (MQ=255)

                                                                                                                                           gATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCg       >  1:1492934/1‑150 (MQ=255)

                                                                                                                                           gATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGAGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCg       >  1:98740/1‑150 (MQ=255)

                                                                                                                                            acggCAAAACGGATCGTTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCgg      >  2:660422/3‑150 (MQ=255)

                                                                                                                                             tGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGa     <  1:1267999/150‑1 (MQ=255)

                                                                                                                                             cggCAAAACGGATCGTTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGATGTACCAGCGAATGATTGCGCTGCGAAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGCTGCTGTATGCGGa     >  1:382086/2‑150 (MQ=255)

                                                                                                                                             cggCAAAACGGATCGTTTTTGCCGTAAACAGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGATGTCAGGTGCTGTATGCGGa     >  1:929060/2‑150 (MQ=255)

                                                                                                                                              gtcataaaCGATCCGTTTTGACGTAAACCGTACCCCTTGCAGGTGGAAAAGCAGGATACCTCGTTATTCTCGCTGTACCAGCGAATGATTGCGCTGCGTAACAAAAGTCAGGCGCTACGTCATTGCGGCTGTCAGGAGCTGTATGCggta    <  2:1658417/145‑3 (MQ=255)

                                                                                                                                              ggCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGaa    <  1:1866899/150‑1 (MQ=255)

                                                                                                                                               gCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAg   >  1:631787/1‑150 (MQ=255)

                                                                                                                                                cAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGANNAGCAGGNTACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGa  >  1:868922/1‑150 (MQ=255)

                                                                                                                                                cAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGa  >  2:1426901/1‑150 (MQ=255)

                                                                                                                                                cAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGa  >  1:649106/1‑150 (MQ=255)

TAATCAGCTCGACAGCCACGATACTGCGCGATTTAAAACGCTGCTCGGTCGGGATATTGCGCGCCTGCCGCTGGCGGTGGTCTGGCTGTTCACCTGGCCTGGTGTACCGTGCATTTATTACGGTGATGAAGTAGGACTGGATGGCAAAAACGATCCGTTTTGCCGTAAACCGTTCCCCTGGCAGGTGGAAAAGCAGGATACGGCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGA  >  NC_000913/423840‑424133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`