Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,728,219	T→A	5.0%	I385F (ATT→TTT)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,728,219	0	T	A	5.0%	247.1 / 2.4	79	I385F (ATT→TTT)	xylB	xylulokinase
Reads supporting (aligned to +/- strand):  ref base T (53/22);  new base A (2/2);  total (55/24)
Fisher's exact test for biased strand distribution p-value = 5.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

GATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGC  >  NC_000913/3728070‑3728364                                                                                                                                                        |                                                                                                                                                  gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTATTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑t                                                                                                                                                   <  2:1172743/150‑1 (MQ=255) gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAACCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTGACACTTTGCGGTTTAA‑t                                                                                                                                                   <  2:1010434/150‑1 (MQ=255)  aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑Ta                                                                                                                                                  <  1:828902/150‑1 (MQ=255)    cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TAcc                                                                                                                                                <  1:1564185/150‑1 (MQ=255)          cAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTATTA‑AACCGCaaag                                                                                                                                          <  1:971816/150‑4 (MQ=255)          cAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTATTA‑AACCGCaaag                                                                                                                                          <  2:1025396/150‑4 (MQ=255)               ttGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCa                                                                                                                                     <  2:629872/150‑1 (MQ=255)               ttGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCa                                                                                                                                     <  2:272760/150‑1 (MQ=255)                   tgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGAc                                                                                                                                 <  1:1572447/150‑1 (MQ=255)                   tgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGAc                                                                                                                                 <  1:156439/150‑1 (MQ=255)                    gcgcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACa                                                                                                                                <  2:859947/150‑1 (MQ=255)                          aGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATg                                                                                                                          <  2:781206/150‑1 (MQ=255)                          aGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATg                                                                                                                          <  2:1402073/150‑1 (MQ=255)                               tGGCCCCACATCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCAt                                                                                                                      <  2:1029897/150‑1 (MQ=255)                                              cccgcGTACGGTAATCGAGCTTCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAc                                                                                                       <  2:293404/150‑1 (MQ=255)                                               ccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAcc                                                                                                      <  1:199448/150‑1 (MQ=255)                                                 ccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCa                                                                                                    >  1:602653/1‑150 (MQ=255)                                                  cGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCAGGCCATCTGCCAGCGCATAACCCAc                                                                                                   >  2:970620/1‑150 (MQ=255)                                                   gTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACg                                                                                                  <  2:8103/150‑1 (MQ=255)                                                      cGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCt                                                                                               >  2:395606/1‑150 (MQ=255)                                                        gTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTc                                                                                             >  2:24067/1‑150 (MQ=255)                                                          aaTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTccc                                                                                           >  2:1216966/1‑149 (MQ=255)                                                            tCGAGCTGCTGACCGCTGATATCCGCCCGCATCTGACCCCAGTACTAACTACGCGCCCCGCCCCCAAACAACGTAACACTTTTCGGATTAA‑TACCGCAGGCATGCACGACAGCCATGCCATCTGCCAGCGCAAACCCCACGCCTTCCAGc                                                                                         >  1:1704973/1‑150 (MQ=255)                                                              gAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCAc                                                                                       >  1:1157741/1‑150 (MQ=255)                                                                gctgctGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCCCGCCTTCCCGCCCTg                                                                                     >  2:235641/1‑150 (MQ=255)                                                                   gctgACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCATGCACCGTAACAATTT‑CGGTTTAATTACCGCGGGCATGCCGGACTTCCAGGCCAGGGGCCAGCGCATACCCCACGCGTTCCGGccaggggg                                                                                  >  2:714355/1‑143 (MQ=255)                                                                        ccGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCCCGCAGTCCACCACGGCTCGCGcc                                                                             >  1:1688685/1‑150 (MQ=255)                                                                        ccGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGcc                                                                             <  2:1860637/150‑1 (MQ=255)                                                                        ccGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCAGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCAAGCGCTTAACCCAAGCCTTCCAGCACTGCTCGCGcc                                                                             >  1:1115351/1‑150 (MQ=255)                                                                           cTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGt                                                                          >  2:852768/1‑150 (MQ=255)                                                                           cTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGt                                                                          >  2:850947/1‑150 (MQ=255)                                                                              atatCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCa                                                                       >  1:1414451/1‑150 (MQ=255)                                                                                atCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCAtt                                                                     >  1:1733398/1‑150 (MQ=255)                                                                                     ccAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGc                                                                <  2:1052095/150‑1 (MQ=255)                                                                                       aGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCa                                                              >  2:1875773/1‑150 (MQ=255)                                                                                          cgcTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGt                                                           <  2:1761458/148‑1 (MQ=255)                                                                                            cTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTtg                                                         >  2:19416/1‑150 (MQ=255)                                                                                             tGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCTCGACTTCCATGCCATCTGCCAGCACATAACCCGCGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTtgg                                                        >  2:1853287/1‑149 (MQ=255)                                                                                             tGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCTGCGCATAAACCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCGATGTtgg                                                        >  2:904368/1‑149 (MQ=255)                                                                                             tGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTtga                                                        >  1:1355974/1‑150 (MQ=255)                                                                                              gACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGGCAGTTCATTGGTGCCATGTtgat                                                       >  2:1213462/1‑150 (MQ=255)                                                                                              gACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTtgat                                                       >  2:1179559/1‑150 (MQ=255)                                                                                                cGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTACCACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATATCCAACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCAGGTtgatga                                                     >  2:534399/1‑150 (MQ=255)                                                                                                cGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTtgatga                                                     >  1:1639575/1‑150 (MQ=255)                                                                                                cGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACACCCGCCTTCCAGCACGGCTCGCGCCAGTGCATTAGGGCCATGTtgatgc                                                     >  1:117880/1‑149 (MQ=255)                                                                                                 gCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAg                                                    <  1:198754/150‑1 (MQ=255)                                                                                                   cAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTc                                                  >  2:368706/1‑150 (MQ=255)                                                                                                       aCTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAAc                                              <  2:685605/150‑1 (MQ=255)                                                                                                        cTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAccc                                             >  2:1538785/1‑150 (MQ=255)                                                                                                        cTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAAcc                                             >  2:369688/1‑150 (MQ=255)                                                                                                         tCACTACGCGACCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCAAGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCa                                            >  1:1440046/1‑150 (MQ=255)                                                                                                          cACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCaa                                           >  2:1082541/1‑150 (MQ=255)                                                                                                          cACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGAATGCACGACATCCATGCCATCTGCCAGCGCATACCCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCaa                                           >  2:1175525/1‑150 (MQ=255)                                                                                                           aCTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGAGGAGTCAAACCaaa                                          >  2:177364/1‑150 (MQ=255)                                                                                                            cTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATACCCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAg                                         >  2:814584/1‑150 (MQ=255)                                                                                                             tACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGa                                        <  1:331312/150‑1 (MQ=255)                                                                                                             tACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGa                                        >  1:1005895/1‑150 (MQ=255)                                                                                                              aCGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGaa                                       <  1:772712/150‑1 (MQ=255)                                                                                                               cgcgCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGaaa                                      >  2:1208020/1‑150 (MQ=255)                                                                                                               cgcgCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGaaa                                      >  1:1107289/1‑150 (MQ=255)                                                                                                                  gccccgccccCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAACAcc                                   >  1:1436183/1‑150 (MQ=255)                                                                                                                        cccccAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTc                             >  2:1724038/1‑150 (MQ=255)                                                                                                                         ccccAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCg                            >  2:1334874/1‑150 (MQ=255)                                                                                                                                 aaCGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGa                    >  1:1726010/1‑150 (MQ=255)                                                                                                                                 aaCGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGa                    >  1:1706958/1‑150 (MQ=255)                                                                                                                                     tAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGAGCCAGTTCATTGGGGCCAACATGATGAGTCAAACCAAAGAAAACCCCCATCGCCTGGGGattat                >  2:1258514/1‑150 (MQ=255)                                                                                                                                      aaCACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGattatt               >  1:1569293/1‑150 (MQ=255)                                                                                                                                        cacTTTGCGGTTTAA‑TACCGCAGGCCTGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAAACACCTTCTCCTGGGCATTATtgt             >  1:72462/1‑150 (MQ=255)                                                                                                                                        cacTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgt             >  1:643435/1‑150 (MQ=255)                                                                                                                                          gcctGCGGTATTA‑AACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgtgg           >  1:1119594/4‑149 (MQ=255)                                                                                                                                          cTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgtgt           >  1:64295/1‑150 (MQ=255)                                                                                                                                          cTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCGGGGGATTATtgtgt           >  2:1627719/1‑150 (MQ=255)                                                                                                                                           cctGCGGTATTA‑AACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCCAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgtgtg          >  2:91694/3‑150 (MQ=255)                                                                                                                                            ttGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCAAACCCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTgg         <  2:1157741/150‑1 (MQ=255)                                                                                                                                              gCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCg       <  2:1414451/150‑1 (MQ=255)                                                                                                                                               cGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGAGTATTGTGTGGCGt      >  1:1479889/1‑150 (MQ=255)                                                                                                                                                ggTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTa     >  2:1084336/1‑150 (MQ=255)                                                                                                                                                ggTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAAACAAATAAAACCCCCTTCGCCTGGTGATTATTGTGTGGCGTa     >  2:177543/1‑150 (MQ=255)                                                                                                                                                 gTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTAc    >  2:1097/1‑150 (MQ=255)                                                                                                                                                  tttAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCACAGACAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACg   >  1:441001/1‑150 (MQ=255)                                                                                                                                                   ttAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGc  >  2:1847875/1‑150 (MQ=255)                                                                                                                                                        |                                                                                                                                                  GATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAA‑TACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGC  >  NC_000913/3728070‑3728364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑