Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 424,873 A→C 5.7% S16A (TCG→GCG)  acpH ← acyl carrier protein (ACP) phosphodiesterase; ACP hydrolyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913424,8730AC 5.7% 346.8 / 8.2 116S16A (TCG→GCG) acpHacyl carrier protein (ACP) phosphodiesterase; ACP hydrolyase
Reads supporting (aligned to +/- strand):  ref base A (55/54);  new base C (5/2);  total (60/56)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

Only 100 of 119 total aligned reads displayed.

AAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGAGTCAGTGTCATGCGCGTTACC  >  NC_000913/424724‑425022
                                                                                                                                                     |                                                                                                                                                     
aaaCCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGCCGTCGGGCGGAAAACTTTCTTCAGGATTTCAGCGTACGAAATCAGCCAGTAAATTGCCGGAAACCGa                                                                                                                                                       >  2:1799463/1‑150 (MQ=255)
  aCCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGc                                                                                                                                                     <  1:79707/150‑1 (MQ=255)
        cccGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCcg                                                                                                                                               >  1:1642639/1‑150 (MQ=255)
        cccGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCcg                                                                                                                                               >  1:1297311/1‑150 (MQ=255)
         ccGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATCCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCcgc                                                                                                                                              >  1:877055/1‑150 (MQ=255)
          cGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCTCGCTTTctggc                                                                                                                                             <  2:1600747/150‑5 (MQ=255)
          cGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCTCGCTTTCcggc                                                                                                                                             <  1:1796334/150‑3 (MQ=255)
            tGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCgag                                                                                                                                           >  2:703261/1‑150 (MQ=255)
              cTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGAt                                                                                                                                         >  1:1868450/1‑150 (MQ=255)
                tCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCCCGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATgg                                                                                                                                       >  1:45488/1‑150 (MQ=255)
                tCGCGGACTTCCGGCAGATTGTCAGACAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATgg                                                                                                                                       <  1:706696/150‑1 (MQ=255)
                 ctcgGACTTCCGGCAGATTGTCAGTCATTACGTCGATACGTCGATGGATATGAATGCTAGACACGAAGTCGGTGTTAATACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGAAAATTGCCGGAAAGCGAGCTTTCCGCGAGTTggg                                                                                                                                      <  1:75784/148‑1 (MQ=255)
                 cgcgGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATggg                                                                                                                                      <  2:1603490/150‑1 (MQ=255)
                  gcgGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGc                                                                                                                                     >  1:1686425/1‑150 (MQ=255)
                     gACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTaa                                                                                                                                  <  1:1209553/150‑1 (MQ=255)
                       cTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAAt                                                                                                                                <  2:442829/150‑1 (MQ=255)
                          ccGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCTGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCa                                                                                                                             <  1:1249486/150‑1 (MQ=255)
                          ccGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCa                                                                                                                             >  2:1484354/1‑150 (MQ=255)
                          ccGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCa                                                                                                                             >  2:1867584/1‑150 (MQ=255)
                          ccGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCa                                                                                                                             >  2:589620/1‑150 (MQ=255)
                           cGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAg                                                                                                                            >  2:1592773/1‑150 (MQ=255)
                            ggCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAgg                                                                                                                           <  2:788051/150‑1 (MQ=255)
                              cAGCTTGTCAGGAAATTCGTCGATCCGTCGATGCATATGAATGCCAGCCCCGACGTCGGGCGGAAAACTTTCTTCAGCATTTCCGGGTACGAAATCAGCCCGTAAATTGCCCGAATGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTg                                                                                                                         <  2:1576475/150‑1 (MQ=255)
                               aGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAATACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGa                                                                                                                        <  2:887254/150‑1 (MQ=255)
                                gATTGTCAGTCAATCCGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAg                                                                                                                       <  2:1171210/150‑1 (MQ=255)
                                    gtcagtcaATCCGTCGATACGTCGCTGCAGATGAATGCCCGCCCCGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTCCGAAATCACCCAGTAAATTGCCGGAACGCGCGCTGTCCGAGAGATGGGCGAAATGCAGGTGCGCTaa                                                                                                                   >  2:1782823/1‑150 (MQ=255)
                                       agtcaATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTaaaaa                                                                                                                <  2:1231876/150‑1 (MQ=255)
                                        gtcaATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTaaaaaa                                                                                                               >  1:1059262/1‑150 (MQ=255)
                                         tcaATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAAt                                                                                                              <  1:1118035/150‑1 (MQ=255)
                                         gcaATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAAt                                                                                                              <  2:1510161/149‑1 (MQ=255)
                                           aaTACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTc                                                                                                            >  2:462135/1‑150 (MQ=255)
                                              acgtcgatacgtcgatGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATc                                                                                                         <  1:962155/150‑1 (MQ=255)
                                                 tcgatacgtcgatGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGAt                                                                                                      >  2:1570928/1‑150 (MQ=255)
                                                  cgatacgtcgatGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGAtt                                                                                                     >  1:128098/1‑150 (MQ=255)
                                                   gatacgtcgatGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATta                                                                                                    >  1:1780729/1‑150 (MQ=255)
                                                      acgtcgatGCATATGAATGCCAGCCACGACGTCGGGCTGAAAACTTTCTACATGATTTCCGAGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATCCAGGTGAGCTAAAAAATTCATCGATAatat                                                                                                 <  2:506113/150‑1 (MQ=255)
                                                       cgtcgatGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATAtt                                                                                                >  2:791986/1‑150 (MQ=255)
                                                       cgtcgatGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGGACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAAGTCATCGATTATAtt                                                                                                >  1:652397/1‑150 (MQ=255)
                                                           gatGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTAt                                                                                            <  1:1110688/150‑1 (MQ=255)
                                                           gatGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGAGAGCTAAAAAATTCATCGATTATATTCTAt                                                                                            >  2:1419740/1‑150 (MQ=255)
                                                            atGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTGTCCGCGAGATGGGCTAAATGCAGGTGAGCTTAAAAATTCATCGATTATATTCTATc                                                                                           <  2:1708868/150‑1 (MQ=255)
                                                              gCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTATATCCa                                                                                         >  2:601819/1‑150 (MQ=255)
                                                               cATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCaa                                                                                        >  2:1058203/1‑150 (MQ=255)
                                                               cATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCaa                                                                                        >  1:118097/1‑150 (MQ=255)
                                                                atatGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCaaa                                                                                       >  1:1734463/1‑150 (MQ=255)
                                                                  atGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAg                                                                                     >  2:7596/1‑150 (MQ=255)
                                                                   tGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAgg                                                                                    >  1:1134381/1‑150 (MQ=255)
                                                                   tGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAgg                                                                                    >  1:434976/1‑150 (MQ=255)
                                                                    taaTTCCAGCCACGACGTCGGGAGTATAACTTTCTTCAGGTTTTCCGCGTACTAAATCAGCCAGTATATTGCCGGAAAGAGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAggg                                                                                   <  2:618509/149‑1 (MQ=255)
                                                                    gAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAggg                                                                                   <  2:613051/150‑1 (MQ=255)
                                                                      aTGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCTGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAggggg                                                                                 <  1:478920/150‑1 (MQ=255)
                                                                      aTGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAggggg                                                                                 <  2:1387501/150‑1 (MQ=255)
                                                                          cagccaCGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTaaa                                                                             >  1:1177588/1‑150 (MQ=255)
                                                                            gccaCGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAgg                                                                           >  1:101987/1‑150 (MQ=255)
                                                                             ccaCGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGt                                                                          <  2:901567/150‑1 (MQ=255)
                                                                             ccaCGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGt                                                                          <  1:284240/150‑1 (MQ=255)
                                                                               acgacgTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTg                                                                        >  2:1366676/1‑150 (MQ=255)
                                                                                 gacgTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCa                                                                      <  1:702687/150‑1 (MQ=255)
                                                                                 gacgTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCa                                                                      <  1:359980/150‑1 (MQ=255)
                                                                                  ccgTCGGGCGGAAAACTTTCTTCAGGCTTACCGCGCACGAAATCAGCCAGTAAATAGCCCGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCTGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAACGTTGCAg                                                                     <  2:148228/149‑1 (MQ=255)
                                                                                  ccgTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAg                                                                     <  2:176131/149‑1 (MQ=255)
                                                                                     tCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGga                                                                  <  2:1044740/150‑1 (MQ=255)
                                                                                       gggCGGAAAACTTTCTTCAGGTTTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTTATGGTTGCAGGgaga                                                                >  2:1421484/1‑150 (MQ=255)
                                                                                            gAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGcc                                                           >  1:308865/1‑150 (MQ=255)
                                                                                                 cTTTGTTCAGGTTTTCCGCTTAAGAAATAAGCCATTAAATTGCCGGAAAGCGAGCTGTCCGCGAGATGGGCTAAATGCAGGTGAGATAAAAAATTCATCTATTATATTCTATCCAAAAGGGTGTAAAGGTTGCAGGGAGAGCGCCCCGGc                                                      <  2:329859/150‑1 (MQ=255)
                                                                                                 cTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGc                                                      >  1:1345589/1‑150 (MQ=255)
                                                                                                 cTTTCATCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGc                                                      <  1:522722/150‑1 (MQ=255)
                                                                                                    ccatcAGGATTTCCGCGCTCGAAAGCAGCCAGTAAATTGCCGCAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGACTATATTCTATCCAAAAGGGCGGAAAGGCTGCAGCGCCCGCGCCCCGGCACt                                                   <  2:121148/147‑1 (MQ=255)
                                                                                                        cAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGAc                                               <  2:1739930/150‑1 (MQ=255)
                                                                                                            aTTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTAcc                                           >  1:1402591/1‑150 (MQ=255)
                                                                                                                  gcgTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCctc                                     <  2:45488/150‑1 (MQ=255)
                                                                                                                   cgcaCGAACACATCCAGTAAATTGCCTGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAATAATTCATCGATTATATTCTATGCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCctct                                    <  2:914116/147‑1 (MQ=255)
                                                                                                                   cgTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAACAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCctct                                    <  2:1657758/150‑1 (MQ=255)
                                                                                                                   cgTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCctct                                    <  1:1512762/150‑1 (MQ=255)
                                                                                                                     tACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTa                                  >  1:1038532/1‑150 (MQ=255)
                                                                                                                       cGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTAtt                                >  2:861877/1‑150 (MQ=255)
                                                                                                                        gAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTAttt                               >  1:1814911/1‑150 (MQ=255)
                                                                                                                         aaaTCTGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTAATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTAtttt                              <  1:939609/150‑1 (MQ=255)
                                                                                                                         aaaTCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCCTCGATTATATTCTATCCAAAAGGGGGTATAGGTTGCAGTGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTAtttt                              <  2:118097/150‑1 (MQ=255)
                                                                                                                         aaaTCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTAtttt                              >  1:1804737/1‑150 (MQ=255)
                                                                                                                            tCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGt                           >  1:1844359/1‑150 (MQ=255)
                                                                                                                               gCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTg                        >  2:52217/1‑150 (MQ=255)
                                                                                                                                ccAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGa                       >  2:7656/1‑150 (MQ=255)
                                                                                                                                 gaGTAAATTGCCGGAAGGCAATATTTTCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGAg                      <  1:177948/149‑1 (MQ=255)
                                                                                                                                 ccgTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGAg                      <  2:128098/148‑1 (MQ=255)
                                                                                                                                 cAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGAg                      >  2:207409/1‑150 (MQ=255)
                                                                                                                                     aaaTTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGAGTCAg                  >  1:1524891/1‑150 (MQ=255)
                                                                                                                                     aaaTTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGAGTCAg                  <  2:608798/150‑1 (MQ=255)
                                                                                                                                     aaaTTGCCGGAAAGCGAGCTATCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGAGTCAg                  <  2:1430242/150‑1 (MQ=255)
                                                                                                                                      aaTTGACGGAAAGCGAGCTTTCCGCGAGATGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGAGTCAgcg                <  1:703261/150‑3 (MQ=255)
                                                                                                                                         tGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGAGTCAGTGTc              <  2:511009/150‑1 (MQ=255)
                                                                                                                                          gCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCGTATTTTAGTCTGAGTCAGTGTCa             >  1:993900/1‑150 (MQ=255)
                                                                                                                                           gcGGAAAGCTCGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGAGTCAGTGTctt            >  1:133620/2‑148 (MQ=255)
                                                                                                                                           gcGGAAAGCTCGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGAGTCAGTGTCAt            >  2:903085/2‑150 (MQ=255)
                                                                                                                                           gcGGAAAGCTCGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGAGTCAGTGTCAt            >  1:1209278/2‑150 (MQ=255)
                                                                                                                                                 aGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGAGTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGAGTCAGTGTCATGCGCGt      >  2:1201719/1‑150 (MQ=255)
                                                                                                                                                  gCGAGCTTTCCGCGCGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGAGTCAGTGTCATGCGCGtt     <  2:1364934/150‑1 (MQ=255)
                                                                                                                                                  gCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGAGTCAGTGTCATGCGCGtt     >  2:1715129/1‑150 (MQ=255)
                                                                                                                                                    taGCTTTCCGCGAGATGTGCTAAATGCCGGTGAGCTAAAATATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGTAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGAGTCAGTGTCATGCGCGTTAc   <  1:1615906/149‑1 (MQ=255)
                                                                                                                                                     aGCTTTCCGCGAGATGGGCTAACTGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGCGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGAGTCAGTGTCATGCGCGTTAcc  <  2:1297311/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCACGACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTCTTATTTTAGTCTGAGTCAGTGTCATGCGCGTTACC  >  NC_000913/424724‑425022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: