Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,741,090 A→C 5.8% intergenic (+132/+19) avtA → / ← ysaA valine‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase/putative hydrogenase, 4Fe‑4S ferredoxin‑type component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,741,0900AC 5.8% 293.5 / 5.6 103intergenic (+132/+19)avtA/ysaAvaline‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase/putative hydrogenase, 4Fe‑4S ferredoxin‑type component
Reads supporting (aligned to +/- strand):  ref base A (51/46);  new base C (2/4);  total (53/50)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01

Only 100 of 107 total aligned reads displayed.

TGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTAAT  >  NC_000913/3740941‑3741237
                                                                                                                                                     |                                                                                                                                                   
tGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTga                                                                                                                                                     <  1:1616123/150‑1 (MQ=255)
 gggCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTgag                                                                                                                                                    <  2:688654/150‑1 (MQ=255)
  ggCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTgaga                                                                                                                                                   <  2:989710/150‑1 (MQ=255)
    cTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGaaa                                                                                                                                                 >  2:1469263/1‑150 (MQ=255)
       aaaGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGc                                                                                                                                              <  2:1129780/150‑1 (MQ=255)
          gTCACTAAAAATTGGCTGTTGCGCAACGCGTATCAGGCCTACATGATTTCTGCAATATATTGAATTAGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTTCTCAACGCCgg                                                                                                                                           <  2:1152802/150‑1 (MQ=18)
          gTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTTCTCAACGCCgg                                                                                                                                           <  2:962113/150‑1 (MQ=255)
           tCACTAAAAATTGCCTGCTGCGCTACGCTTATCAGGCCTACATGATTTCTTCAATATATTGAATTTGGAAGAATTTGTAGGCCGTATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTTCTCAACGCCGGa                                                                                                                                          <  1:1398586/150‑1 (MQ=18)
           tCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGACTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTTCTCAACGCCGGa                                                                                                                                          <  1:159568/150‑1 (MQ=18)
             aCTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaa                                                                                                                                        >  1:198410/1‑150 (MQ=255)
             aCTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaa                                                                                                                                        >  2:1753331/1‑150 (MQ=255)
             aCTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaa                                                                                                                                        >  2:1795913/1‑150 (MQ=255)
             aCTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaa                                                                                                                                        >  1:438187/1‑150 (MQ=255)
              cTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACTCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAACCGCCGGaaaa                                                                                                                                       >  2:1217285/1‑150 (MQ=255)
              cTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCTCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaaa                                                                                                                                       >  2:607144/1‑150 (MQ=255)
              cTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaaa                                                                                                                                       >  2:1147798/1‑150 (MQ=255)
              cTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaaa                                                                                                                                       >  2:1397616/1‑150 (MQ=255)
                aaaaaTTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACg                                                                                                                                     >  1:575201/1‑150 (MQ=255)
                   aaTTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGttt                                                                                                                                  <  2:663340/150‑1 (MQ=255)
                     ttGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCa                                                                                                                                <  1:1045826/150‑1 (MQ=255)
                      cgCCCGATCCGCTACGCTTAGCAGGCCTACATGACTTCTGCAATAAATTGAATATGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAACCGCCGGAAAACGTTTCaa                                                                                                                               <  2:1849570/149‑1 (MQ=21)
                          tGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAg                                                                                                                           <  2:1099788/150‑1 (MQ=255)
                          tGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAg                                                                                                                           >  2:170054/1‑150 (MQ=255)
                          tGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAg                                                                                                                           >  1:305339/1‑150 (MQ=255)
                           gATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAgg                                                                                                                          >  2:880356/1‑150 (MQ=255)
                            aTGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGgc                                                                                                                         <  2:1109677/150‑1 (MQ=255)
                            aTGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTACCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGgc                                                                                                                         <  1:763815/150‑1 (MQ=255)
                                   aCGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGcc                                                                                                                  <  2:1326115/150‑1 (MQ=255)
                                      cTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTa                                                                                                               >  1:1440291/1‑150 (MQ=255)
                                      cTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCCGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCTTTGAGAAACGCCGGAAAACGTTTCAAACACACTGCTGCCGTa                                                                                                               >  1:274652/1‑150 (MQ=255)
                                        tATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGc                                                                                                             >  2:1288742/1‑150 (MQ=255)
                                         aTCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAGCGTTTCAAACAGGCTGCTGCCGTAGcc                                                                                                            <  2:869906/150‑1 (MQ=255)
                                         aTCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGcc                                                                                                            <  2:212427/150‑1 (MQ=255)
                                           cAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATATGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGa                                                                                                          <  2:26345/150‑1 (MQ=255)
                                           cAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGTCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGa                                                                                                          >  2:971962/1‑150 (MQ=255)
                                            aGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGAt                                                                                                         <  1:269484/150‑1 (MQ=255)
                                             ggCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGACGCATCTGGCATAAACAAAGCGCACTGTAACGGCGTTGAGAAACGCAGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATg                                                                                                        >  2:1068665/1‑150 (MQ=255)
                                              gCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTACGGCAGCAGcctgttt                                                                                                       <  2:825340/150‑6 (MQ=25)
                                               ccTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGcc                                                                                                      <  1:1272641/150‑1 (MQ=255)
                                                 tACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGc                                                                                                    >  2:1344982/1‑150 (MQ=255)
                                                   aaTGATATCTGCAATAAATTGAATTTGGAATAATTTGTCGGCCGTATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGGTCCGGCGTTGAGAACCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTg                                                                                                  <  1:366754/149‑1 (MQ=37)
                                                      gATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCAc                                                                                               <  2:1480481/150‑1 (MQ=255)
                                                        tttCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACtt                                                                                             >  1:149018/1‑150 (MQ=255)
                                                        tttCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACtt                                                                                             >  1:167549/1‑150 (MQ=255)
                                                        tttCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAAGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGGTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACtt                                                                                             <  1:1825035/150‑1 (MQ=255)
                                                            tGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTtctc                                                                                         <  2:1322872/150‑1 (MQ=255)
                                                              cAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGCAAACGTTTCAAACAGGCTGCTCCCGTAGCCGATGCCGCTGCACATTCTCGa                                                                                       >  2:1148084/1‑150 (MQ=255)
                                                               aaTATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGAc                                                                                      >  1:1797736/1‑150 (MQ=255)
                                                               aaTATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCAGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGAc                                                                                      <  2:862252/150‑1 (MQ=255)
                                                                atatatTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACa                                                                                     <  1:331963/150‑1 (MQ=255)
                                                                  atatTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATc                                                                                   >  1:483808/1‑150 (MQ=255)
                                                                   tatTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATcc                                                                                  <  2:1786266/150‑1 (MQ=255)
                                                                    atTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCa                                                                                 <  2:1160274/150‑1 (MQ=255)
                                                                    atTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCa                                                                                 >  1:1252326/1‑150 (MQ=255)
                                                                    atTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCa                                                                                 >  2:484874/1‑150 (MQ=255)
                                                                    atTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCa                                                                                 >  2:1305843/1‑150 (MQ=255)
                                                                     ttGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCAt                                                                                >  1:1598826/1‑150 (MQ=255)
                                                                      tGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATg                                                                               >  2:1124762/1‑150 (MQ=255)
                                                                       gAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCCTGc                                                                              >  2:1162714/1‑150 (MQ=255)
                                                                       gAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGc                                                                              <  1:1288742/150‑1 (MQ=255)
                                                                         aTTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATACATTCAc                                                                            >  1:1184237/1‑150 (MQ=255)
                                                                               aagaaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAACACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAAc                                                                      >  1:132456/1‑150 (MQ=255)
                                                                               aagaaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAAc                                                                      >  1:688317/1‑150 (MQ=255)
                                                                                agaaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCTACATCCATGCACTGCAACg                                                                     >  1:950769/1‑150 (MQ=255)
                                                                                      tGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTg                                                               >  2:424278/1‑150 (MQ=255)
                                                                                      tGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTg                                                               <  2:1261975/150‑1 (MQ=255)
                                                                                         aGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACCTCCATGTACTGCAACGCCTTTGTCg                                                            >  2:1107586/1‑150 (MQ=255)
                                                                                          ggCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCCCTGCAACGCCTTTGTCgg                                                           >  1:944299/1‑150 (MQ=255)
                                                                                          ggCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCgg                                                           >  2:749264/1‑150 (MQ=255)
                                                                                             cGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCa                                                        <  1:1585368/150‑1 (MQ=255)
                                                                                              ggATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAg                                                       >  2:676395/1‑150 (MQ=255)
                                                                                                  aaGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCtt                                                   <  2:220911/150‑1 (MQ=255)
                                                                                                   aGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTc                                                  <  2:377705/150‑1 (MQ=255)
                                                                                                   aGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTACTCGACATCCATGCACTGCAACGCCATTGTCGGGCAGGCGTc                                                  >  1:49766/1‑150 (MQ=255)
                                                                                                    ggCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCa                                                 <  2:1536993/150‑1 (MQ=255)
                                                                                                    ggCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGACTTTGTCGGGCAGGCTTCa                                                 <  2:1589279/150‑1 (MQ=255)
                                                                                                        tttACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAacac                                             <  2:1550483/150‑1 (MQ=255)
                                                                                                            cgccgcATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGc                                         <  1:617876/150‑1 (MQ=255)
                                                                                                                     tGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGTTCCCGt                                >  1:142544/1‑150 (MQ=255)
                                                                                                                     tGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGt                                >  1:1717393/1‑150 (MQ=255)
                                                                                                                        cATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGtctc                             >  1:1179685/1‑150 (MQ=255)
                                                                                                                         aTAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTcc                            <  2:1440291/150‑1 (MQ=255)
                                                                                                                           aaaCAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCg                          <  2:1233209/150‑1 (MQ=255)
                                                                                                                             aCAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGAt                        <  1:484874/150‑1 (MQ=255)
                                                                                                                               aaaGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGCCATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGc                      >  1:1604730/1‑150 (MQ=255)
                                                                                                                                aaGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGcc                     <  1:391713/150‑1 (MQ=255)
                                                                                                                                    gcACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGCCATCCATGCACTGCAACGCCTTTGTCGGGCCGGCTTCCACCCCGGCCGGTCCCGTCGCCCGATGCCCGcc                 >  1:1693648/1‑149 (MQ=255)
                                                                                                                                    gcACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCACCGGCTGTGTCGGGCCGGCTGCAACACCGGGCGGTGCCGTCTCCCGATGGCAGcc                 >  2:299311/1‑149 (MQ=255)
                                                                                                                                    gcACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCGGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACCCAGGCCGGTCCCGTCTCCCGATGCCAGca                 <  2:305339/150‑1 (MQ=255)
                                                                                                                                       gttttCCGGCGTTTCTCAACGCCGGAAAACGTTTCAAGCAGGCTGCTGCCGTAGCCGATGCCGCTGCGCGTTCTCGCCATCCATGCACTGCAAAGGCTGTGTCGGGCAGGCGTCCACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAg              >  1:1166512/4‑150 (MQ=255)
                                                                                                                                           tCCGGCGTTTCTCAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCg          >  2:1300527/1‑150 (MQ=255)
                                                                                                                                           tCCGGCGTTGAGAACCGCCGGAAATCGTTTCAAACAGGCTGCTGCCGTAGCCGCTGCCGCTGCACTTTCTCGACATCCATGCCCTGCAACGCCTTTGTCGGGCAGGCTTCAACCCAGGCCGGTCCCGTCTCCCGATGGCCGGACAGCTCg          >  2:1458217/1‑150 (MQ=255)
                                                                                                                                            ccGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGTAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGc         >  1:1239290/1‑150 (MQ=255)
                                                                                                                                            ccGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGCTGCCAGCACAGATCGc         >  1:1179822/1‑150 (MQ=255)
                                                                                                                                            ccGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGc         <  2:1569018/150‑1 (MQ=255)
                                                                                                                                              ggCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGAGATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACTCAGGCCGGTCCCGTCTCCCGATGCCAGCACCGATCGCAc       >  1:98738/1‑150 (MQ=255)
                                                                                                                                               gCGTTTAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACt      <  1:1455486/150‑1 (MQ=255)
                                                                                                                                               gCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGTAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACt      <  2:1239290/150‑1 (MQ=255)
                                                                                                                                               gCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACt      <  2:1702679/150‑1 (MQ=255)
                                                                                                                                                   tGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCTACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTAAt  <  2:1313080/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTAAT  >  NC_000913/3740941‑3741237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: