Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,741,091 G→T 5.5% intergenic (+133/+18) avtA → / ← ysaA valine‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase/putative hydrogenase, 4Fe‑4S ferredoxin‑type component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,741,0910GT 5.5% 306.6 / 5.2 103intergenic (+133/+18)avtA/ysaAvaline‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase/putative hydrogenase, 4Fe‑4S ferredoxin‑type component
Reads supporting (aligned to +/- strand):  ref base G (51/46);  new base T (2/4);  total (53/50)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01

Only 100 of 109 total aligned reads displayed.

GGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTAATCGC  >  NC_000913/3740942‑3741240
                                                                                                                                                     |                                                                                                                                                     
gggCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTgag                                                                                                                                                       <  2:688654/150‑1 (MQ=255)
 ggCTGAAAGTCACTAATAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTgaga                                                                                                                                                      <  2:1009859/150‑1 (MQ=255)
 ggCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTgaga                                                                                                                                                      <  2:989710/150‑1 (MQ=255)
   cTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGaaa                                                                                                                                                    >  2:1469263/1‑150 (MQ=255)
      aaaGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGc                                                                                                                                                 <  2:1129780/150‑1 (MQ=255)
         gTCACTAAAAATTGGCTGTTGCGCAACGCGTATCAGGCCTACATGATTTCTGCAATATATTGAATTAGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTTCTCAACGCCgg                                                                                                                                              <  2:1152802/150‑1 (MQ=18)
         gTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTTCTCAACGCCgg                                                                                                                                              <  2:962113/150‑1 (MQ=255)
          tCACTAAAAATTGCCTGCTGCGCTACGCTTATCAGGCCTACATGATTTCTTCAATATATTGAATTTGGAAGAATTTGTAGGCCGTATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTTCTCAACGCCGGa                                                                                                                                             <  1:1398586/150‑1 (MQ=18)
          tCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGACTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTTCTCAACGCCGGa                                                                                                                                             <  1:159568/150‑1 (MQ=18)
            aCTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaa                                                                                                                                           >  1:438187/1‑150 (MQ=255)
            aCTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaa                                                                                                                                           >  1:198410/1‑150 (MQ=255)
            aCTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaa                                                                                                                                           >  2:1753331/1‑150 (MQ=255)
             cTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACTCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAACCGCCGGaaaa                                                                                                                                          >  2:1217285/1‑150 (MQ=255)
             cTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaaa                                                                                                                                          >  2:1147798/1‑150 (MQ=255)
             cTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaaa                                                                                                                                          >  2:1397616/1‑150 (MQ=255)
               aaaaaTTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACg                                                                                                                                        >  1:575201/1‑150 (MQ=255)
                 aatttGCCTTATGCGCTACGCTTATCAGGCCTACATGTTTTCTCAAATATATTTATTTTGTAATAATTTGTTGGCCGTATACGGCGTTTACGCCGCTTCTGGAATAAACATCGCGCACTGTTCCGGCTTTTAGAACCGCGGGAAAACGtt                                                                                                                                      <  2:1561108/147‑1 (MQ=16)
                  aaTTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGttt                                                                                                                                     <  2:663340/150‑1 (MQ=255)
                   aTTGCCTGATGCGCTACGCTTATCAGGCGTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACATAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTc                                                                                                                                    <  1:308687/150‑1 (MQ=255)
                    ttGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCa                                                                                                                                   <  1:1045826/150‑1 (MQ=255)
                     cgCCCGATCCGCTACGCTTAGCAGGCCTACATGACTTCTGCAATAAATTGAATATGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAACCGCCGGAAAACGTTTCaa                                                                                                                                  <  2:1849570/149‑1 (MQ=21)
                         tGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAg                                                                                                                              >  2:170054/1‑150 (MQ=255)
                         tGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAg                                                                                                                              >  1:305339/1‑150 (MQ=255)
                         tGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAg                                                                                                                              <  2:1099788/150‑1 (MQ=255)
                          gATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAgg                                                                                                                             >  2:880356/1‑150 (MQ=255)
                           aTGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTACCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGgc                                                                                                                            <  1:763815/150‑1 (MQ=255)
                                  aCGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGcc                                                                                                                     <  2:1326115/150‑1 (MQ=255)
                                     cTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCCGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCTTTGAGAAACGCCGGAAAACGTTTCAAACACACTGCTGCCGTa                                                                                                                  >  1:274652/1‑150 (MQ=255)
                                       tATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGc                                                                                                                >  2:1288742/1‑150 (MQ=255)
                                       tATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGc                                                                                                                >  2:391713/1‑150 (MQ=255)
                                        aTCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAGCGTTTCAAACAGGCTGCTGCCGTAGcc                                                                                                               <  2:869906/150‑1 (MQ=255)
                                        aTCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGcc                                                                                                               <  2:212427/150‑1 (MQ=255)
                                          cAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATATGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGa                                                                                                             <  2:26345/150‑1 (MQ=255)
                                          cAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGTCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGa                                                                                                             >  2:971962/1‑150 (MQ=255)
                                           aGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGAt                                                                                                            <  1:269484/150‑1 (MQ=255)
                                            ggCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGACGCATCTGGCATAAACAAAGCGCACTGTAACGGCGTTGAGAAACGCAGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATg                                                                                                           >  2:1068665/1‑150 (MQ=255)
                                             gCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTACGGCAGCAGcctgttt                                                                                                          <  2:825340/150‑6 (MQ=25)
                                              ccTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGcc                                                                                                         <  1:1272641/150‑1 (MQ=255)
                                                tACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGc                                                                                                       >  2:1344982/1‑150 (MQ=255)
                                                  aaTGATATCTGCAATAAATTGAATTTGGAATAATTTGTCGGCCGTATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGGTCCGGCGTTGAGAACCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTg                                                                                                     <  1:366754/149‑1 (MQ=37)
                                                     gATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCAc                                                                                                  <  2:1480481/150‑1 (MQ=255)
                                                       tttCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACtt                                                                                                >  1:167549/1‑150 (MQ=255)
                                                       tttCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACtt                                                                                                >  1:149018/1‑150 (MQ=255)
                                                           tGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTtctc                                                                                            <  2:1322872/150‑1 (MQ=255)
                                                             cAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGCAAACGTTTCAAACAGGCTGCTCCCGTAGCCGATGCCGCTGCACATTCTCGa                                                                                          >  2:1148084/1‑150 (MQ=255)
                                                              aaTATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGAc                                                                                         >  1:1797736/1‑150 (MQ=255)
                                                              aaTATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCAGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGAc                                                                                         <  2:862252/150‑1 (MQ=255)
                                                               atatatTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACa                                                                                        <  1:331963/150‑1 (MQ=255)
                                                                 atatTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATc                                                                                      >  1:483808/1‑150 (MQ=255)
                                                                  tatTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATcc                                                                                     <  2:1786266/150‑1 (MQ=255)
                                                                   atTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCa                                                                                    >  2:1305843/1‑150 (MQ=255)
                                                                   atTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCa                                                                                    >  2:484874/1‑150 (MQ=255)
                                                                   atTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCa                                                                                    <  2:1160274/150‑1 (MQ=255)
                                                                   atTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCa                                                                                    >  1:1252326/1‑150 (MQ=255)
                                                                    ttGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCAt                                                                                   >  1:1598826/1‑150 (MQ=255)
                                                                     tGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATg                                                                                  >  2:1124762/1‑150 (MQ=255)
                                                                      gAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCCTGc                                                                                 >  2:1162714/1‑150 (MQ=255)
                                                                      gAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGc                                                                                 <  1:1288742/150‑1 (MQ=255)
                                                                        aTTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATACATTCAc                                                                               >  1:1184237/1‑150 (MQ=255)
                                                                              aagaaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAACACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAAc                                                                         >  1:132456/1‑150 (MQ=255)
                                                                              aagaaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAAc                                                                         >  1:688317/1‑150 (MQ=255)
                                                                               agaaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCTACATCCATGCACTGCAACg                                                                        >  1:950769/1‑150 (MQ=255)
                                                                                     tGTAGGCCGGATACGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCAGTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTg                                                                  <  2:127338/150‑1 (MQ=255)
                                                                                     tGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTg                                                                  >  2:424278/1‑150 (MQ=255)
                                                                                        tggCCGGATAAGGCGTTTACGCCGCATCTGGCAAAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCg                                                               <  1:1534900/149‑1 (MQ=255)
                                                                                        aGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACCTCCATGTACTGCAACGCCTTTGTCg                                                               >  2:1107586/1‑150 (MQ=255)
                                                                                         ggCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCCCTGCAACGCCTTTGTCgg                                                              >  1:944299/1‑150 (MQ=255)
                                                                                         ggCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCgg                                                              >  2:749264/1‑150 (MQ=255)
                                                                                            cGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCa                                                           <  1:1585368/150‑1 (MQ=255)
                                                                                             ggATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAg                                                          >  2:676395/1‑150 (MQ=255)
                                                                                                 aaGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCtt                                                      <  2:220911/150‑1 (MQ=255)
                                                                                                  aGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTc                                                     <  2:377705/150‑1 (MQ=255)
                                                                                                   ggCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCa                                                    <  2:1536993/150‑1 (MQ=255)
                                                                                                   ggCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGACTTTGTCGGGCAGGCTTCa                                                    <  2:1589279/150‑1 (MQ=255)
                                                                                                       tttACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAacac                                                <  2:1550483/150‑1 (MQ=255)
                                                                                                           cgccgcATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGc                                            <  1:617876/150‑1 (MQ=255)
                                                                                                                    tGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGTTCCCGt                                   >  1:142544/1‑150 (MQ=255)
                                                                                                                    tGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGt                                   >  1:1717393/1‑150 (MQ=255)
                                                                                                                       cATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGtctc                                >  1:1179685/1‑150 (MQ=255)
                                                                                                                        aTAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTcc                               <  2:1440291/150‑1 (MQ=255)
                                                                                                                          aaaCAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCg                             <  2:1233209/150‑1 (MQ=255)
                                                                                                                            aCAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGAt                           <  1:484874/150‑1 (MQ=255)
                                                                                                                              aaaGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGCCATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGc                         >  1:1604730/1‑150 (MQ=255)
                                                                                                                               aaGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGcc                        <  1:391713/150‑1 (MQ=255)
                                                                                                                                   gcACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGCCATCCATGCACTGCAACGCCTTTGTCGGGCCGGCTTCCACCCCGGCCGGTCCCGTCGCCCGATGCCCGcc                    >  1:1693648/1‑149 (MQ=255)
                                                                                                                                   gcACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCACCGGCTGTGTCGGGCCGGCTGCAACACCGGGCGGTGCCGTCTCCCGATGGCAGcc                    >  2:299311/1‑149 (MQ=255)
                                                                                                                                   gcACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCGGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACCCAGGCCGGTCCCGTCTCCCGATGCCAGca                    <  2:305339/150‑1 (MQ=255)
                                                                                                                                      gttttCCGGCGTTTCTCAACGCCGGAAAACGTTTCAAGCAGGCTGCTGCCGTAGCCGATGCCGCTGCGCGTTCTCGCCATCCATGCACTGCAAAGGCTGTGTCGGGCAGGCGTCCACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAg                 >  1:1166512/4‑150 (MQ=255)
                                                                                                                                          tCCGGCGTTTCTCAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCg             >  2:1300527/1‑150 (MQ=255)
                                                                                                                                           ccGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGTAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGc            >  1:1239290/1‑150 (MQ=255)
                                                                                                                                           ccGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGCTGCCAGCACAGATCGc            >  1:1179822/1‑150 (MQ=255)
                                                                                                                                           ccGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGc            <  2:1569018/150‑1 (MQ=255)
                                                                                                                                             ggCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGAGATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACTCAGGCCGGTCCCGTCTCCCGATGCCAGCACCGATCGCAc          >  1:98738/1‑150 (MQ=255)
                                                                                                                                              gCGTTTAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACt         <  1:1455486/150‑1 (MQ=255)
                                                                                                                                              gCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGTAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACt         <  2:1239290/150‑1 (MQ=255)
                                                                                                                                              gCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACt         <  2:1702679/150‑1 (MQ=255)
                                                                                                                                                  tGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCTACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTAAt     <  2:1313080/150‑1 (MQ=255)
                                                                                                                                                     gaAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCCGCACAGATCGCACTTAATCGc  >  2:1393338/1‑150 (MQ=255)
                                                                                                                                                     gaAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTAATCGc  <  2:1252326/150‑1 (MQ=255)
                                                                                                                                                     gaAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGACCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTAATCGc  >  2:364954/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTAATCGC  >  NC_000913/3740942‑3741240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: