Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,741,092 A→C 5.5% intergenic (+134/+17) avtA → / ← ysaA valine‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase/putative hydrogenase, 4Fe‑4S ferredoxin‑type component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,741,0920AC 5.5% 312.2 / 5.8 102intergenic (+134/+17)avtA/ysaAvaline‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase/putative hydrogenase, 4Fe‑4S ferredoxin‑type component
Reads supporting (aligned to +/- strand):  ref base A (50/46);  new base C (2/4);  total (52/50)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

Only 100 of 109 total aligned reads displayed.

GGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTAATCGCC  >  NC_000913/3740943‑3741241
                                                                                                                                                     |                                                                                                                                                     
ggCTGAAAGTCACTAATAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTgaga                                                                                                                                                       <  2:1009859/150‑1 (MQ=255)
ggCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTgaga                                                                                                                                                       <  2:989710/150‑1 (MQ=255)
  cTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGaaa                                                                                                                                                     >  2:1469263/1‑150 (MQ=255)
     aaaGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGc                                                                                                                                                  <  2:1129780/150‑1 (MQ=255)
        gTCACTAAAAATTGGCTGTTGCGCAACGCGTATCAGGCCTACATGATTTCTGCAATATATTGAATTAGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTTCTCAACGCCgg                                                                                                                                               <  2:1152802/150‑1 (MQ=18)
        gTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTTCTCAACGCCgg                                                                                                                                               <  2:962113/150‑1 (MQ=255)
         tCACTAAAAATTGCCTGCTGCGCTACGCTTATCAGGCCTACATGATTTCTTCAATATATTGAATTTGGAAGAATTTGTAGGCCGTATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTTCTCAACGCCGGa                                                                                                                                              <  1:1398586/150‑1 (MQ=18)
         tCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGACTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTTCTCAACGCCGGa                                                                                                                                              <  1:159568/150‑1 (MQ=18)
           aCTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaa                                                                                                                                            >  1:438187/1‑150 (MQ=255)
           aCTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaa                                                                                                                                            >  2:1795913/1‑150 (MQ=255)
           aCTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaa                                                                                                                                            >  2:1753331/1‑150 (MQ=255)
           aCTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaa                                                                                                                                            >  1:198410/1‑150 (MQ=255)
            cTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACTCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAACCGCCGGaaaa                                                                                                                                           >  2:1217285/1‑150 (MQ=255)
            cTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCTCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaaa                                                                                                                                           >  2:607144/1‑150 (MQ=255)
            cTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaaa                                                                                                                                           >  2:1147798/1‑150 (MQ=255)
            cTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaaa                                                                                                                                           >  2:1397616/1‑150 (MQ=255)
              aaaaaTTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACg                                                                                                                                         >  1:575201/1‑150 (MQ=255)
                aatttGCCTTATGCGCTACGCTTATCAGGCCTACATGTTTTCTCAAATATATTTATTTTGTAATAATTTGTTGGCCGTATACGGCGTTTACGCCGCTTCTGGAATAAACATCGCGCACTGTTCCGGCTTTTAGAACCGCGGGAAAACGtt                                                                                                                                       <  2:1561108/147‑1 (MQ=16)
                 aaTTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGttt                                                                                                                                      <  2:663340/150‑1 (MQ=255)
                  aTTGCCTGATGCGCTACGCTTATCAGGCGTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACATAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTc                                                                                                                                     <  1:308687/150‑1 (MQ=255)
                   ttGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCa                                                                                                                                    <  1:1045826/150‑1 (MQ=255)
                    cgCCCGATCCGCTACGCTTAGCAGGCCTACATGACTTCTGCAATAAATTGAATATGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAACCGCCGGAAAACGTTTCaa                                                                                                                                   <  2:1849570/149‑1 (MQ=21)
                        tGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAg                                                                                                                               >  2:170054/1‑150 (MQ=255)
                        tGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAg                                                                                                                               <  2:1099788/150‑1 (MQ=255)
                        tGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAg                                                                                                                               >  1:305339/1‑150 (MQ=255)
                         gATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAgg                                                                                                                              >  2:880356/1‑150 (MQ=255)
                          aTGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGgc                                                                                                                             <  2:1109677/150‑1 (MQ=255)
                          aTGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTACCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGgc                                                                                                                             <  1:763815/150‑1 (MQ=255)
                                 aCGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGcc                                                                                                                      <  2:1326115/150‑1 (MQ=255)
                                    cTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCCGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCTTTGAGAAACGCCGGAAAACGTTTCAAACACACTGCTGCCGTa                                                                                                                   >  1:274652/1‑150 (MQ=255)
                                      tATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGc                                                                                                                 >  2:391713/1‑150 (MQ=255)
                                      tATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGc                                                                                                                 >  2:1288742/1‑150 (MQ=255)
                                       aTCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAGCGTTTCAAACAGGCTGCTGCCGTAGcc                                                                                                                <  2:869906/150‑1 (MQ=255)
                                       aTCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGcc                                                                                                                <  2:212427/150‑1 (MQ=255)
                                         cAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATATGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGa                                                                                                              <  2:26345/150‑1 (MQ=255)
                                         cAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGTCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGa                                                                                                              >  2:971962/1‑150 (MQ=255)
                                          aGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGAt                                                                                                             <  1:269484/150‑1 (MQ=255)
                                           ggCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGACGCATCTGGCATAAACAAAGCGCACTGTAACGGCGTTGAGAAACGCAGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATg                                                                                                            >  2:1068665/1‑150 (MQ=255)
                                            gCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTACGGCAGCAGcctgttt                                                                                                           <  2:825340/150‑6 (MQ=25)
                                             ccTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGcc                                                                                                          <  1:1272641/150‑1 (MQ=255)
                                               tACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGc                                                                                                        >  2:1344982/1‑150 (MQ=255)
                                                 aaTGATATCTGCAATAAATTGAATTTGGAATAATTTGTCGGCCGTATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGGTCCGGCGTTGAGAACCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTg                                                                                                      <  1:366754/149‑1 (MQ=37)
                                                    gATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCAc                                                                                                   <  2:1480481/150‑1 (MQ=255)
                                                      tttCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACtt                                                                                                 >  1:167549/1‑150 (MQ=255)
                                                      tttCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACtt                                                                                                 >  1:149018/1‑150 (MQ=255)
                                                      tttCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAAGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGGTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACtt                                                                                                 <  1:1825035/150‑1 (MQ=255)
                                                          tGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTtctc                                                                                             <  2:1322872/150‑1 (MQ=255)
                                                            cAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGCAAACGTTTCAAACAGGCTGCTCCCGTAGCCGATGCCGCTGCACATTCTCGa                                                                                           >  2:1148084/1‑150 (MQ=255)
                                                             aaTATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCAGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGAc                                                                                          <  2:862252/150‑1 (MQ=255)
                                                              atatatTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACa                                                                                         <  1:331963/150‑1 (MQ=255)
                                                                atatTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATc                                                                                       >  1:483808/1‑150 (MQ=255)
                                                                  atTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCa                                                                                     >  1:1252326/1‑150 (MQ=255)
                                                                  atTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCa                                                                                     >  2:1305843/1‑150 (MQ=255)
                                                                  atTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCa                                                                                     <  2:1160274/150‑1 (MQ=255)
                                                                   ttGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCAt                                                                                    >  1:1598826/1‑150 (MQ=255)
                                                                    tGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATg                                                                                   >  2:1124762/1‑150 (MQ=255)
                                                                     gAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCCTGc                                                                                  >  2:1162714/1‑150 (MQ=255)
                                                                     gAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGc                                                                                  <  1:1288742/150‑1 (MQ=255)
                                                                       aTTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATACATTCAc                                                                                >  1:1184237/1‑150 (MQ=255)
                                                                             aagaaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAACACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAAc                                                                          >  1:132456/1‑150 (MQ=255)
                                                                             aagaaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAAc                                                                          >  1:688317/1‑150 (MQ=255)
                                                                              agaaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCTACATCCATGCACTGCAACg                                                                         >  1:950769/1‑150 (MQ=255)
                                                                                    tGTAGGCCGGATACGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCAGTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTg                                                                   <  2:127338/150‑1 (MQ=255)
                                                                                    tGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTg                                                                   <  2:1261975/150‑1 (MQ=255)
                                                                                    tGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTg                                                                   >  2:424278/1‑150 (MQ=255)
                                                                                       tggCCGGATAAGGCGTTTACGCCGCATCTGGCAAAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCg                                                                <  1:1534900/149‑1 (MQ=255)
                                                                                        ggCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCCCTGCAACGCCTTTGTCgg                                                               >  1:944299/1‑150 (MQ=255)
                                                                                        ggCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCgg                                                               >  2:749264/1‑150 (MQ=255)
                                                                                           cGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCa                                                            <  1:1585368/150‑1 (MQ=255)
                                                                                            ggATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAg                                                           >  2:676395/1‑150 (MQ=255)
                                                                                                aaGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCtt                                                       <  2:220911/150‑1 (MQ=255)
                                                                                                 aGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTc                                                      <  2:377705/150‑1 (MQ=255)
                                                                                                 aGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTACTCGACATCCATGCACTGCAACGCCATTGTCGGGCAGGCGTc                                                      >  1:49766/1‑150 (MQ=255)
                                                                                                  ggCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCa                                                     <  2:1536993/150‑1 (MQ=255)
                                                                                                  ggCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGACTTTGTCGGGCAGGCTTCa                                                     <  2:1589279/150‑1 (MQ=255)
                                                                                                      tttACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAacac                                                 <  2:1550483/150‑1 (MQ=255)
                                                                                                          cgccgcATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGc                                             <  1:617876/150‑1 (MQ=255)
                                                                                                                   tGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGTTCCCGt                                    >  1:142544/1‑150 (MQ=255)
                                                                                                                   tGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGt                                    >  1:1717393/1‑150 (MQ=255)
                                                                                                                      cATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGtctc                                 >  1:1179685/1‑150 (MQ=255)
                                                                                                                         aaaCAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCg                              <  2:1233209/150‑1 (MQ=255)
                                                                                                                             aaaGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGCCATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGc                          >  1:1604730/1‑150 (MQ=255)
                                                                                                                              aaGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGcc                         <  1:391713/150‑1 (MQ=255)
                                                                                                                                  gcACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGCCATCCATGCACTGCAACGCCTTTGTCGGGCCGGCTTCCACCCCGGCCGGTCCCGTCGCCCGATGCCCGcc                     >  1:1693648/1‑149 (MQ=255)
                                                                                                                                  gcACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCACCGGCTGTGTCGGGCCGGCTGCAACACCGGGCGGTGCCGTCTCCCGATGGCAGcc                     >  2:299311/1‑149 (MQ=255)
                                                                                                                                  gcACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCGGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACCCAGGCCGGTCCCGTCTCCCGATGCCAGca                     <  2:305339/150‑1 (MQ=255)
                                                                                                                                     gttttCCGGCGTTTCTCAACGCCGGAAAACGTTTCAAGCAGGCTGCTGCCGTAGCCGATGCCGCTGCGCGTTCTCGCCATCCATGCACTGCAAAGGCTGTGTCGGGCAGGCGTCCACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAg                  >  1:1166512/4‑150 (MQ=255)
                                                                                                                                         tCCGGCGTTTCTCAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCg              >  2:1300527/1‑150 (MQ=255)
                                                                                                                                         tCCGGCGTTGAGAACCGCCGGAAATCGTTTCAAACAGGCTGCTGCCGTAGCCGCTGCCGCTGCACTTTCTCGACATCCATGCCCTGCAACGCCTTTGTCGGGCAGGCTTCAACCCAGGCCGGTCCCGTCTCCCGATGGCCGGACAGCTCg              >  2:1458217/1‑150 (MQ=255)
                                                                                                                                          ccGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGTAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGc             >  1:1239290/1‑150 (MQ=255)
                                                                                                                                          ccGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGCTGCCAGCACAGATCGc             >  1:1179822/1‑150 (MQ=255)
                                                                                                                                          ccGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGc             <  2:1569018/150‑1 (MQ=255)
                                                                                                                                            ggCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGAGATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACTCAGGCCGGTCCCGTCTCCCGATGCCAGCACCGATCGCAc           >  1:98738/1‑150 (MQ=255)
                                                                                                                                             gCGTTTAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACt          <  1:1455486/150‑1 (MQ=255)
                                                                                                                                             gCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGTAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACt          <  2:1239290/150‑1 (MQ=255)
                                                                                                                                             gCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACt          <  2:1702679/150‑1 (MQ=255)
                                                                                                                                                 tGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCTACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTAAt      <  2:1313080/150‑1 (MQ=255)
                                                                                                                                                    gaAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCCGCACAGATCGCACTTAATCGc   >  2:1393338/1‑150 (MQ=255)
                                                                                                                                                    gaAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGACCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTAATCGc   >  2:364954/1‑150 (MQ=255)
                                                                                                                                                     aaaCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCAGAGATCGCACTTAATCGcc  >  1:1707938/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTAATCGCC  >  NC_000913/3740943‑3741241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: