Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,131 T→G 8.6% intergenic (+114/‑84) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,1310TG 8.6% 181.6 / 2.9 82intergenic (+114/‑84)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (40/32);  new base G (3/5);  total (43/39)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGC  >  NC_000913/3780982‑3781280
                                                                                                                                                     |                                                                                                                                                     
gCACTGGCTCCCATGGCGAAAGGGTATGCTGCATAGTCTTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCt                                                                                                                                                       <  2:1738914/150‑1 (MQ=255)
      gCTCCCTTGGCGAAAGGGAATTCGTCATTGTCGTTTGCGCCCCCTCACCCTAAGCGTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTGCCTCGCCCCTCCgggg                                                                                                                                                 <  1:785167/150‑1 (MQ=255)
      gCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgggg                                                                                                                                                 <  1:1344214/150‑1 (MQ=255)
        acacATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgag                                                                                                                                               <  2:916472/147‑1 (MQ=255)
         cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCGAACCCACTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTTTGTTCTCCCTCACTCGGGAACGCCCCCCCCCTATTTTTTTATCCCTCGCCCCGCCggggggg                                                                                                                                              >  2:410588/1‑147 (MQ=255)
            aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggg                                                                                                                                           <  1:631611/150‑1 (MQ=255)
            aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggg                                                                                                                                           <  2:1090887/150‑1 (MQ=255)
             tGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGc                                                                                                                                          <  2:217287/150‑1 (MQ=255)
                cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGTGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                                                                                                                       <  1:1642771/150‑1 (MQ=255)
                 gAACGGGAATGCGGCATAGTCGACCGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                                                                                      <  1:225379/150‑1 (MQ=255)
                 gAAAGGGATTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                                                                                      <  2:1587926/150‑1 (MQ=255)
                 gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGTAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                                                                                      <  2:1580442/150‑1 (MQ=255)
                 gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTACCTCGCCCCTCCGGGGAGAGGGCCggg                                                                                                                                      >  2:1059318/1‑150 (MQ=255)
                    aGGGAAGGCGGCATAGGCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTg                                                                                                                                   <  1:1532/150‑1 (MQ=255)
                     gggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCAAACCCACTCCCTCAGGGAGAGGGGAACGTTCGGCGCTGTATGTACTCCCTCACACTGAAACGACACCCCACTCATTTTTTCTCCCTCCCCCCTCCGGGGAGACGGCCGGGGTGa                                                                                                                                  >  2:1029626/1‑150 (MQ=255)
                       gAACGCGGCATCGGCGTTTGCGCCCCCTCACCCTACCCCTCGCCCTCAGGGAGCGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                                                <  1:1870078/150‑1 (MQ=255)
                        aaTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAggg                                                                                                                               <  2:1720786/150‑1 (MQ=255)
                         aTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgggg                                                                                                                              <  1:1069874/150‑1 (MQ=255)
                            cgtcATAGTCGTTTGCGCCCCCTCACCCTATCCCTCTCCCTCATGGCGAGGGGACCGTTCGGCGCTGTATGTAATCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCACCTCCTGGGAGAGGGCCGGGGTGAGGGGaaa                                                                                                                           <  2:811811/147‑1 (MQ=255)
                              gCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTGCCTCAGGGAGTGGGGACCGTTCGGCGCGGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAg                                                                                                                         <  2:355133/150‑1 (MQ=255)
                              gCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGGATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAg                                                                                                                         <  2:2547/150‑1 (MQ=255)
                              gCATAGTCGTTGGCGCCCCCTCACCCAAACCCACTCCACCCGGGCGAGGGGACCGTTCGGCGCTGAATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAg                                                                                                                         <  1:1673602/150‑1 (MQ=255)
                                 ttgTCGTTTGCGCCCCCTCACCATAACCCTCTCCCTCTGGGAGAGGGTACCGTTCGGCGCTGTATTTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGTGGGCCGGGGTGAGGGGAAAAGGcc                                                                                                                      <  2:527973/148‑1 (MQ=255)
                                           cgcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGcgcc                                                                                                            >  2:870782/1‑147 (MQ=255)
                                           cgcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCGTTTTTTCTCCCTCGCCCCGCCGGGGAGAGGGCCGGGGTGAGGGGATAAGGCCGCACTGGTCa                                                                                                            >  1:1368550/1‑150 (MQ=255)
                                                 cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAtt                                                                                                      >  1:1343180/1‑150 (MQ=255)
                                                        gtccccTCTCCCTGAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGCAAAAGGCCGCACTGCTCACCAATTTTCTGCt                                                                                               >  2:538960/4‑150 (MQ=255)
                                                          aCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCAGTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCAACAATTTTCTGCTAt                                                                                             >  2:578094/1‑150 (MQ=255)
                                                            ccTCTCCCTCATGGAGAGGGGACCGTTCGGCGCTGTAAGTACTCCCTCACTCTGAAACGAAACCGCACTCTTTTTTACTCCCTCGCCCCTCCGGTTAGAGGTCCGGGGTGAGGGCAAAACGCCGCACAGCTCCCCAATTTTCTGCTAttc                                                                                           >  2:881024/1‑148 (MQ=255)
                                                            ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                                           >  2:1693216/1‑150 (MQ=255)
                                                            ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                                           >  1:759808/1‑150 (MQ=255)
                                                            ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGACCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                                           <  2:1399314/150‑1 (MQ=255)
                                                             ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGACCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCCCCAATTTTCTGCTATCCt                                                                                          >  2:1618349/1‑150 (MQ=255)
                                                                 cccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTCCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccc                                                                                      >  2:630776/1‑150 (MQ=255)
                                                                 cccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCCCTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGTAAAGGCCGCACTGCTCCCCAATTTTCTGCTATCCTGccc                                                                                      >  2:237686/1‑150 (MQ=255)
                                                                          agagGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGCCACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAATTTCTGCTATCCTGCCCCCGCACTaa                                                                             >  2:1167739/1‑150 (MQ=255)
                                                                            agGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgg                                                                           >  1:1700330/1‑150 (MQ=255)
                                                                             ggggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggg                                                                          >  1:1624440/1‑150 (MQ=255)
                                                                                  ccGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACCCCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGCGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTGAGCAATTTTCTGCTATCCGGCACCAGCACGAAGGGGGCAg                                                                     >  2:1746740/1‑150 (MQ=255)
                                                                                    gTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTa                                                                   >  1:617369/1‑150 (MQ=255)
                                                                                    gTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTa                                                                   >  1:743847/1‑150 (MQ=255)
                                                                                     ttCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt                                                                  >  2:354060/1‑150 (MQ=255)
                                                                                     tgcGGCGCTGGATCCACACCCTCACACTGACCCCCCCCCCCACCCTTTTTATCTCCCGCGCCCCTCCGGGGAGCGGGCCGGGGGGCGGGTACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCTTAGTGCGGGGGCAGGAt                                                                  <  1:304691/148‑1 (MQ=255)
                                                                                       cGGCGCTTTATGTCCCCCCCCACTCTTAAACCCCCCCCCACTGTTTTTTTCCCCCCCCCCCCGCCGGGGAGTGGGCGGGGGGGCGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGc                                                                <  2:1632942/150‑1 (MQ=255)
                                                                                                 tgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGCGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCCCTAAGGGGGCAGTATGCTAACCATCGt                                                      >  2:1659490/1‑150 (MQ=255)
                                                                                                  gtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATATTCTGCTTTCCTGCCCCCGCACTAAGGGGGCAGTCTGCTAAGCATCGtc                                                     >  2:95755/1‑149 (MQ=255)
                                                                                                   taCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGTGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTAc                                                    >  1:893781/1‑150 (MQ=255)
                                                                                                    aatCCCTCAATCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACt                                                   <  2:217729/148‑1 (MQ=255)
                                                                                                      tCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGCGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTCAACATCGTCCttt                                                 >  2:1616393/1‑150 (MQ=255)
                                                                                                        ccTCACTCTCAACCGCCCCCGCACGCTTTTTTTCTCCCGCGCCCCTCCGGGTAGTGGGCCGGGGTGCGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                                               <  1:1267614/150‑1 (MQ=255)
                                                                                                             ctctGAACCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGATAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc                                          <  2:84269/150‑1 (MQ=255)
                                                                                                             ctctGAAACGACACAGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAACCATAGTACATTACGAAcc                                          >  1:967462/1‑150 (MQ=255)
                                                                                                               tttaaaCTACACCTCACTCTTTTTTTCTCCCGCGCCCCTCCGGGGAGAGGGCCGGGGTGTGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg                                        <  2:759808/147‑1 (MQ=255)
                                                                                                               ctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg                                        >  2:511455/1‑150 (MQ=255)
                                                                                                                tGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTTAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGa                                       >  1:663809/1‑150 (MQ=255)
                                                                                                                 gAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGaa                                      <  2:43671/150‑1 (MQ=255)
                                                                                                                   acgaccacCGCCCTGTTTTTTTCACCCGCCCCCCCCCGGGGTGCGGGCGGGGGTGCGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAAt                                    <  2:324812/145‑1 (MQ=255)
                                                                                                                   aaCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGATAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCCGTCTGCTAAACCTCGGACTGTACGGACCAGAAAt                                    >  2:1357729/1‑150 (MQ=255)
                                                                                                                   aaCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAAt                                    >  2:1394361/1‑150 (MQ=255)
                                                                                                                    aCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAAtt                                   >  1:1469971/1‑150 (MQ=255)
                                                                                                                     cGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTc                                  >  2:1871046/1‑150 (MQ=255)
                                                                                                                        cacCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGc                               >  2:451211/1‑150 (MQ=255)
                                                                                                                          cccaaCTCTTTTTTTCCCCCGCCCCCCTCCGGGTCTTGGGCGGGGGTGTGTGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCa                             <  2:1407988/146‑1 (MQ=255)
                                                                                                                          ccGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACGAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCa                             >  1:1183477/1‑150 (MQ=255)
                                                                                                                          ccGCACGCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCa                             <  2:279957/150‑1 (MQ=255)
                                                                                                                                tctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACt                       >  2:1385227/1‑150 (MQ=255)
                                                                                                                                 tttttttcCTCCCGCGCCCCTCGGGGTTGTGGGCCGGGGGGTGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTg                      <  2:1368550/149‑1 (MQ=255)
                                                                                                                                 ctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAACCATCGTAGTTTACGAACCAGAAATTCCGCCAAATACTg                      >  1:529883/1‑150 (MQ=255)
                                                                                                                                 ctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTg                      >  1:1616784/1‑150 (MQ=255)
                                                                                                                                 ctTATTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTACACATCGTACTTTACGAACCAGAAATTCCGCCAAATCCTg                      >  1:1291211/1‑150 (MQ=255)
                                                                                                                                  tttttttCTCCCTCGCCCCTCGGGGTTGTGGGCGGGGGTTTGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                     <  1:1814092/150‑1 (MQ=255)
                                                                                                                                   tttttgCCCGCTGGCCCCGCGGGGGTTTGGGCCGGGGTGTGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGc                    <  2:443865/150‑1 (MQ=255)
                                                                                                                                    ttttgCGCCCGCCCCCCGCCGGGGTGCGGGCGGGGGGGTGTGGACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCa                   <  2:1411861/150‑1 (MQ=255)
                                                                                                                                         cacccTCCCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatc              <  1:337100/148‑1 (MQ=255)
                                                                                                                                          tcCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatca             >  2:1830334/1‑150 (MQ=255)
                                                                                                                                           cccTCTCCCCGGAGGGGCGAGGGCCGGGGTGAGGGGAAAAGGCCACACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatcat            >  1:965042/1‑150 (MQ=255)
                                                                                                                                            ccTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACatcatc           >  1:1526697/1‑150 (MQ=255)
                                                                                                                                            ccTCCCCCCCCGGGGGAGTGGGCGGGGGTGCGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACatcatc           <  1:650291/150‑1 (MQ=255)
                                                                                                                                              cccccccTCGGGGGAGTGGGCGGGGGTGTGGGCACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCg         <  1:538960/147‑1 (MQ=255)
                                                                                                                                              cccccccTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCg         <  1:244866/147‑1 (MQ=255)
                                                                                                                                                cccccccCGGGGATAGGGCCGGGGTGTGGGGACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTc       <  2:926847/149‑1 (MQ=255)
                                                                                                                                                 ccccTCCGGGGAGTGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCt      <  2:1252680/150‑1 (MQ=255)
                                                                                                                                                   ccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCttt    >  1:50008/1‑150 (MQ=255)
                                                                                                                                                   ccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCACCGCACTAATGGGGCAGTATGCTAAAAATCGTACTTTAAGAACCAGAAATTCCGCCAAATAATGGCAACATAATCCGTCttt    >  1:336820/1‑150 (MQ=255)
                                                                                                                                                     tCCGTGGAGAGGGCCGGGGTGAGGGGAACATGCAGCACTGCGCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGTTAAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCttttc  <  2:100364/150‑3 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGC  >  NC_000913/3780982‑3781280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: