Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,811,212 C→T 5.4% intergenic (‑60/+38) mutM ← / ← rpmG formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase/50S ribosomal subunit protein L33

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,811,2120CT 5.4% 463.0 / 9.8 148intergenic (‑60/+38)mutM/rpmGformamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase/50S ribosomal subunit protein L33
Reads supporting (aligned to +/- strand):  ref base C (63/77);  new base T (3/5);  total (66/82)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-02

Only 100 of 150 total aligned reads displayed.

TGCGCACCACTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAG  >  NC_000913/3811065‑3811358
                                                                                                                                                   |                                                                                                                                                  
tGCGCACCACTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCccg                                                                                                                                                  <  1:29244/150‑1 (MQ=255)
  cgcACCACTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCccgcc                                                                                                                                                >  2:1408319/1‑150 (MQ=255)
      ccacTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAg                                                                                                                                            <  2:1574952/150‑1 (MQ=255)
        acTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCg                                                                                                                                          >  1:1748664/1‑150 (MQ=255)
           gCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAgg                                                                                                                                       <  1:1243339/150‑1 (MQ=255)
                 agaaTGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGtttttt                                                                                                                                 >  2:1640148/1‑150 (MQ=255)
                  gaaTGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCTCGCTCCGGCGGGGTTTTTTg                                                                                                                                <  2:1311249/150‑1 (MQ=255)
                  gaaTGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTg                                                                                                                                <  1:1833435/150‑1 (MQ=255)
                  gaaTGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTg                                                                                                                                <  1:1482344/150‑1 (MQ=255)
                  caatgcgtGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTg                                                                                                                                <  2:974954/143‑1 (MQ=255)
                  caaTGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTg                                                                                                                                <  2:1137410/149‑1 (MQ=255)
                  caaTGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACACCGCCGGAGCGAGGTTTTTTg                                                                                                                                <  1:341643/149‑1 (MQ=255)
                   aaTGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCTCGCTCCGGCGGGGTTTTTTGt                                                                                                                               <  1:1409129/150‑1 (MQ=255)
                   aaTGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCTCGCTCCGGCGGGGTTTTTTGt                                                                                                                               <  2:704245/150‑1 (MQ=255)
                   aaTGGTTGCACCAACGAGATGCGGGTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCTCGCTCCGGCGGGGTTTTTTGt                                                                                                                               <  1:975473/150‑1 (MQ=255)
                     tGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTa                                                                                                                             <  1:1325532/150‑1 (MQ=255)
                     tGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTa                                                                                                                             <  2:905114/150‑1 (MQ=255)
                     tGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTa                                                                                                                             <  2:1728916/150‑1 (MQ=255)
                     tGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTa                                                                                                                             <  2:1217477/150‑1 (MQ=255)
                      ggTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTAc                                                                                                                            <  1:1162786/150‑1 (MQ=255)
                       gTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACa                                                                                                                           <  2:1585112/150‑1 (MQ=255)
                       gTTGCACCAACGAGATGCGGTTCTATGACGCGGCGGCTGGTTTCTCTTCGGGTAATTCAGGCATAGCATCTCAAGGAATGAACAGATGCAGTCAATATGGGGGCATGCAGATTACAAAAAACCCCGCCGGAGCGACGTATTTTTTTACAt                                                                                                                          >  2:1853481/1‑150 (MQ=255)
                           cACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCaa                                                                                                                       <  1:1865865/150‑1 (MQ=255)
                             ccAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAg                                                                                                                     >  1:1728497/1‑150 (MQ=255)
                                  gagaTGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCgaga                                                                                                                <  1:1739039/150‑1 (MQ=255)
                                   agaTGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCTGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGaa                                                                                                               >  2:717158/1‑150 (MQ=255)
                                      tGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAatta                                                                                                            <  1:711697/150‑1 (MQ=255)
                                        cGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAattatt                                                                                                          <  2:64537/150‑1 (MQ=255)
                                        cGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAattatt                                                                                                          <  1:595074/150‑1 (MQ=255)
                                         ggTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTAttt                                                                                                         <  1:170489/150‑1 (MQ=255)
                                           ttCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGa                                                                                                       <  1:1198734/150‑1 (MQ=255)
                                            tCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGAt                                                                                                      >  2:808985/1‑150 (MQ=255)
                                                 gCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCg                                                                                                 <  1:603530/150‑1 (MQ=255)
                                                 gCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCg                                                                                                 <  1:1472112/150‑1 (MQ=255)
                                                        cggcTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGTAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCttt                                                                                          <  2:1264142/150‑1 (MQ=255)
                                                         ggcTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGTGCAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCGAAGCGAAAATCAAATAATTCTCGctttgatg                                                                                         <  1:1128023/150‑6 (MQ=255)
                                                         ggcTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAAAATCAAATAATTCTCGctttgatg                                                                                         <  1:229250/150‑6 (MQ=255)
                                                         ggcTGGTTCCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTg                                                                                         <  1:1557985/150‑1 (MQ=255)
                                                           cTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTa                                                                                       <  2:902291/150‑1 (MQ=255)
                                                            tGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGTAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAg                                                                                      <  2:1804387/150‑1 (MQ=255)
                                                            tGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAg                                                                                      <  1:1498816/150‑1 (MQ=255)
                                                                 tCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCAc                                                                                 >  2:1116989/1‑150 (MQ=255)
                                                                        cGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTgg                                                                          >  2:1171817/1‑150 (MQ=255)
                                                                         gggTAATTCAGGCATCGCATCACCAGGAATGCACAGATGCACTCAATATGGGGGCTAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGAGCACGAGCTGGc                                                                         <  2:309239/150‑1 (MQ=255)
                                                                         gggTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGc                                                                         >  1:1321226/1‑150 (MQ=255)
                                                                          ggTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCg                                                                        <  2:1867241/150‑1 (MQ=255)
                                                                             aaTTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGaac                                                                     <  2:1044212/150‑1 (MQ=255)
                                                                             aaTTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGaac                                                                     >  1:987392/1‑150 (MQ=255)
                                                                              aTTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGaaca                                                                    >  1:190078/1‑150 (MQ=255)
                                                                              aTTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCCAGGTTTTTTCTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGaaca                                                                    >  1:955217/1‑150 (MQ=255)
                                                                               ttCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGaacaa                                                                   >  1:642212/1‑150 (MQ=255)
                                                                               ttCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGaacaa                                                                   >  1:387203/1‑150 (MQ=255)
                                                                                 cAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACt                                                                 >  1:1551958/1‑150 (MQ=255)
                                                                                 cAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACt                                                                 >  1:540110/1‑150 (MQ=255)
                                                                                 cAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACt                                                                 >  1:468824/1‑150 (MQ=255)
                                                                                  aGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTg                                                                >  1:111295/1‑150 (MQ=255)
                                                                                  aGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTg                                                                <  1:800364/150‑1 (MQ=255)
                                                                                    gCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGATTTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGa                                                              <  1:1245391/150‑1 (MQ=255)
                                                                                      aTAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATc                                                            <  1:836054/150‑1 (MQ=255)
                                                                                      aTAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATc                                                            <  2:445688/150‑1 (MQ=255)
                                                                                      aTAGCAGCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATc                                                            <  2:128284/150‑1 (MQ=255)
                                                                                       tAGCATCTCCAGGAATGAACAGATGCATTAAATATGGGGGCAAGCAGATAACAATAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCg                                                           <  1:6461/150‑1 (MQ=255)
                                                                                          cATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAt                                                        >  2:482162/1‑150 (MQ=255)
                                                                                          cATCTCCAGGAATGAACAGATGCAGTCAATATGGGCGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGAGCGAAt                                                        <  1:1465018/150‑1 (MQ=255)
                                                                                           aTCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAtt                                                       >  1:1747112/1‑150 (MQ=255)
                                                                                             ctcCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAtttt                                                     >  1:874738/1‑150 (MQ=255)
                                                                                              tcCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAttttt                                                    <  1:613661/150‑1 (MQ=255)
                                                                                               ccAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAtttttt                                                   >  2:979815/1‑150 (MQ=255)
                                                                                               ccAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTCCTGGCGAACAACTGGATCGAAtttttt                                                   >  2:164714/1‑150 (MQ=255)
                                                                                                cAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACGCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTc                                                  <  2:1513969/150‑1 (MQ=255)
                                                                                                cAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTc                                                  <  1:452007/150‑1 (MQ=255)
                                                                                                   gAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGt                                               <  1:834474/150‑1 (MQ=255)
                                                                                                    aaTGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCTAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATATTTTCAGtt                                              >  1:218852/1‑150 (MQ=255)
                                                                                                    aaTAAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGtt                                              >  2:790650/1‑150 (MQ=255)
                                                                                                        aaCAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAg                                          <  1:1846839/150‑1 (MQ=255)
                                                                                                           aGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGttt                                       >  2:1504852/1‑150 (MQ=255)
                                                                                                            gATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGtttt                                      <  2:1702750/150‑1 (MQ=255)
                                                                                                               gCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTcc                                   <  1:904703/150‑1 (MQ=255)
                                                                                                                         tGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTAc                         <  1:1684337/150‑1 (MQ=255)
                                                                                                                         tGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTAc                         >  1:599280/1‑150 (MQ=255)
                                                                                                                             ggCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGttt                     <  2:1047242/150‑1 (MQ=255)
                                                                                                                             ggCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGttt                     <  2:1476820/150‑1 (MQ=255)
                                                                                                                              gaaaGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTg                    <  1:136289/148‑1 (MQ=255)
                                                                                                                              gCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTg                    <  2:133376/150‑1 (MQ=255)
                                                                                                                               cAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGt                   >  1:757653/1‑150 (MQ=255)
                                                                                                                                  gCAGATAACAAAAAACCCCGCCGGAGCGAGGGTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGGACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGttct                <  2:1735126/150‑1 (MQ=255)
                                                                                                                                   cAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGttctt               <  1:1223262/150‑1 (MQ=255)
                                                                                                                                    atgtAACAAAAAACCTCGCTCCGGCGGGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGttcttc              >  2:1604142/4‑150 (MQ=38)
                                                                                                                                    aGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGttcttc              <  2:1252442/150‑1 (MQ=255)
                                                                                                                                    aGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGttcttc              >  2:175938/1‑150 (MQ=255)
                                                                                                                                     gATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCg             >  2:716080/1‑150 (MQ=255)
                                                                                                                                          cAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTTGTACGTTTGTTCTTCGTAGTg        >  2:1144469/1‑150 (MQ=255)
                                                                                                                                          cAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTg        >  2:1712540/1‑150 (MQ=255)
                                                                                                                                          cAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTg        >  1:1356300/1‑150 (MQ=255)
                                                                                                                                          cAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTg        <  2:1032772/150‑1 (MQ=255)
                                                                                                                                           aaaaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTgg       <  2:1482729/150‑1 (MQ=255)
                                                                                                                                            aaaaaCCCCTCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTTTTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGt      <  1:714244/150‑1 (MQ=255)
                                                                                                                                            aaaaaCCCCTCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGt      <  2:288337/150‑1 (MQ=255)
                                                                                                                                            aaaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGt      <  1:1436372/150‑1 (MQ=255)
                                                                                                                                                aCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGCACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAg  >  1:1078989/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGCGCACCACTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAG  >  NC_000913/3811065‑3811358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: