Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,817,948 G→T 7.5% E63D (GAG→GAT dinD → DNA damage‑inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,817,9480GT 7.5% 309.8 / 12.2 106E63D (GAG→GATdinDDNA damage‑inducible protein
Reads supporting (aligned to +/- strand):  ref base G (49/49);  new base T (6/2);  total (55/51)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

Only 100 of 112 total aligned reads displayed.

ATTAATGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGAC  >  NC_000913/3817799‑3818097
                                                                                                                                                     |                                                                                                                                                     
aTTAATGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCgaggag                                                                                                                                                       <  2:1546932/150‑1 (MQ=255)
 ttAATGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGgaga                                                                                                                                                      >  2:90004/1‑150 (MQ=255)
        aaaCGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGa                                                                                                                                               <  1:395301/150‑1 (MQ=255)
          aCGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGata                                                                                                                                             >  2:1658591/1‑150 (MQ=255)
           cGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGatat                                                                                                                                            <  2:1149905/150‑1 (MQ=255)
             gAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCATAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATgg                                                                                                                                          <  2:634753/150‑1 (MQ=255)
             gAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATgg                                                                                                                                          <  2:591978/150‑1 (MQ=255)
               ccAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATATCGAGGATCTCCTCGAAATGGTc                                                                                                                                        <  1:656744/149‑1 (MQ=255)
                 aGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTTTCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACGATTGATGACCATTTCGAGGAGATCCTCGATATGGTCaa                                                                                                                                      >  1:944685/1‑150 (MQ=255)
                 aGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACATTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCaa                                                                                                                                      >  2:404739/1‑150 (MQ=255)
                 aGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCaa                                                                                                                                      <  2:204301/150‑1 (MQ=255)
                       aTGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTgg                                                                                                                                >  1:1282766/1‑150 (MQ=255)
                       aTGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTgg                                                                                                                                <  1:723035/150‑1 (MQ=255)
                        tGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGc                                                                                                                               <  2:674102/150‑1 (MQ=255)
                        tGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGc                                                                                                                               <  2:64954/150‑1 (MQ=255)
                        tGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGc                                                                                                                               <  1:1027164/150‑1 (MQ=255)
                          ggcAGCAAGACAAATTGGGAAACAAATTGGTTATTCATAGGATCGTGACTTTATAACTTTATTAAAGAGCGCCCAAGAAGCCTGTGAGAACAGTCGTCACACAATTGATGACCATTTAGAGGAGATCCTCGAAATGGTCAAAATTTGCta                                                                                                                             >  2:931530/3‑149 (MQ=255)
                            cAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCaa                                                                                                                           >  2:1309639/1‑150 (MQ=255)
                            cAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCaa                                                                                                                           >  2:411554/1‑150 (MQ=255)
                                 aGACAACTTGCCAAAATACTGGTATATACATAGTATCGTCACTTTATACCTGTATTTACGCCCCCCCACGAAGCCTGTGAGAACAGTTGTCACACATATGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGcc                                                                                                                      <  2:1746464/150‑1 (MQ=255)
                                          gggAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCa                                                                                                             <  1:741706/150‑1 (MQ=255)
                                          gggAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCa                                                                                                             >  1:1319098/1‑150 (MQ=255)
                                                ctactGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTaaaa                                                                                                       <  1:1176035/150‑1 (MQ=255)
                                                ctactGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTaaaa                                                                                                       >  1:952444/1‑150 (MQ=255)
                                                   ctGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGAc                                                                                                    <  2:868525/150‑1 (MQ=255)
                                                     gggTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACAt                                                                                                  >  1:1074178/1‑150 (MQ=255)
                                                       gTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATct                                                                                                >  1:229388/1‑149 (MQ=255)
                                                          aTTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAATAAGCCTGTGAGAAAAGTGGTCACACAATTGATGCCCATTTCGCGGAGCTCCTCGATATGGTCAAAATTGGCTCAAATGACAAAAGAGCATTAAAAGACATCGTAc                                                                                             >  1:308993/1‑150 (MQ=255)
                                                                tgTATCGTCACTTTATACCTGTATTAACGCGCGCCTAAGAAGCCTGTGAGAACAGTGGTCACACATTTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTccc                                                                                       <  2:565563/149‑1 (MQ=255)
                                                                  tATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGATAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCATAAGAGCATTAAAAGACATCGTACTCTCCCGc                                                                                     <  1:942350/150‑1 (MQ=255)
                                                                  tATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGc                                                                                     <  1:1446565/150‑1 (MQ=255)
                                                                  tATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGc                                                                                     <  2:423635/150‑1 (MQ=255)
                                                                   aTCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCt                                                                                    <  1:1194454/150‑1 (MQ=255)
                                                                   aTCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACAATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGACAAAAGAGCATTAAAAGACATCGTACTCTCCCGCt                                                                                    >  1:1650466/1‑150 (MQ=255)
                                                                    tCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTa                                                                                   <  1:1658591/150‑1 (MQ=255)
                                                                     cGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTTATGACCAATTCGAGGAGATCCTCGATATGGTCAAAATAGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTAt                                                                                  >  2:792811/1‑150 (MQ=255)
                                                                     cGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTAt                                                                                  >  2:960919/1‑150 (MQ=255)
                                                                       tCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGc                                                                                <  1:1697618/150‑1 (MQ=255)
                                                                         aCTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACGATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCt                                                                              <  2:944685/150‑1 (MQ=255)
                                                                         aCTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCAATTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCt                                                                              <  2:289024/150‑1 (MQ=255)
                                                                          cTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTg                                                                             <  2:1277297/150‑1 (MQ=255)
                                                                            ttATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGtt                                                                           >  1:537450/1‑150 (MQ=255)
                                                                              ataCCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTAGATATGGTCAAAATTGGCTCAAATGCAAAAAGAGCATTAAAAGCCATCGTACTCTCCCGCTATGCCTGTGAc                                                                         >  1:1681405/1‑150 (MQ=255)
                                                                                 ccTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAATACATCGTACTCTCCCGCTATGCCTGTTACCTg                                                                      >  1:245528/1‑150 (MQ=255)
                                                                                 ccTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTg                                                                      >  1:880775/1‑150 (MQ=255)
                                                                                  cTGTATTAACGCGCGCCAAAGAAGCCTGTGATAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTgg                                                                     <  2:1131471/150‑1 (MQ=255)
                                                                                      aTTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgtagt                                                                 >  1:1451921/1‑150 (MQ=255)
                                                                                      aTTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgtagt                                                                 <  2:847746/150‑1 (MQ=255)
                                                                                        tAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTAc                                                               <  2:1519735/150‑1 (MQ=255)
                                                                                         aaCGCGCGCCAAAGAAGCCTTTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACa                                                              <  2:1547667/150‑1 (MQ=255)
                                                                                               cgcCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCACATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACgg                                                        >  2:1671436/1‑150 (MQ=255)
                                                                                                tctgaagaagCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGTTAGTACAAAACGGc                                                       >  1:139419/5‑150 (MQ=255)
                                                                                                gcCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGc                                                       <  1:49020/150‑1 (MQ=255)
                                                                                                gcCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCGCGATATGGTCAAAATTGGCTCAAATGCCAAAAGCGCATTAAAAGCCATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGc                                                       >  1:491234/1‑150 (MQ=255)
                                                                                                 ccAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCg                                                      >  1:186456/1‑150 (MQ=255)
                                                                                                   aaaGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGAc                                                    <  1:484295/150‑1 (MQ=255)
                                                                                                   aaaGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCCTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGCCgcc                                                    >  2:1146032/1‑148 (MQ=255)
                                                                                                    aagaagCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGAcc                                                   <  2:1241494/150‑1 (MQ=255)
                                                                                                     agaagCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGAccc                                                  >  2:64045/1‑150 (MQ=255)
                                                                                                      gaagCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCTTTAAAAGCCATCGTACTCTCCCGCTATGCATGTTACCTGGTTGTACAAAACGGCGCCCCt                                                 >  2:302764/1‑150 (MQ=255)
                                                                                                        agCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGc                                               >  1:1361459/1‑150 (MQ=255)
                                                                                                        agCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGc                                               <  1:1547960/150‑1 (MQ=255)
                                                                                                             gtgAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAAc                                          <  1:1615732/150‑1 (MQ=255)
                                                                                                                agaACAGTTGTCACACAATTGATGAGCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCGTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCgg                                       <  1:303642/150‑1 (MQ=255)
                                                                                                                 gaACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGt                                      >  1:1752998/1‑150 (MQ=255)
                                                                                                                   aCAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCa                                    >  2:1349411/1‑150 (MQ=255)
                                                                                                                   aCAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCa                                    >  2:1692282/1‑150 (MQ=255)
                                                                                                                    cAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCAt                                   <  1:1084669/150‑1 (MQ=255)
                                                                                                                     aGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCAtt                                  >  2:525634/1‑150 (MQ=255)
                                                                                                                     aGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCAtt                                  >  2:1717611/1‑150 (MQ=255)
                                                                                                                       gggTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGc                                <  2:708530/149‑1 (MQ=255)
                                                                                                                         gTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCgg                              >  1:1658539/1‑150 (MQ=255)
                                                                                                                          acacacaATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACTGCGACCCTTCGAAACCGGTCATTGCggc                             <  1:90004/149‑1 (MQ=255)
                                                                                                                            acacaATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAAATTGATCAAATCACAAAAGAGCATTAAAACAAAAAATACTATCCCGCTATGCCTGTTACCTGGTAGTACAAAAAGGCGACCCTGCGAAACCGGGAATTGCggcag                           >  2:1086326/1‑150 (MQ=255)
                                                                                                                             cacaATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGCCCCTGCGAAACCGGTCATTGCggcagg                          >  2:1783600/1‑150 (MQ=255)
                                                                                                                              acaATGGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACAGCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcaggg                         <  1:1188046/150‑1 (MQ=255)
                                                                                                                               caATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcagggc                        <  2:1043519/150‑1 (MQ=255)
                                                                                                                               caATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTATCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTCCGAAACCGGTAATTGCggcagggc                        >  2:1411857/1‑150 (MQ=255)
                                                                                                                               aaaTTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAGGAGCATAAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGAAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcagggc                        <  1:633650/149‑1 (MQ=255)
                                                                                                                                aaTTGATGACCATTTCGAGGAGATCCTCGATATTGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcagggca                       <  1:839248/150‑1 (MQ=255)
                                                                                                                                    gatgaCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACt                   >  2:1700334/1‑150 (MQ=255)
                                                                                                                                    gatgaCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACAGCGTACTCTACCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACGCTGCGAACCCGGTCATTGCGGCAGCGCAGACt                   >  2:1121087/1‑150 (MQ=255)
                                                                                                                                     ttgaCCATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAGAAGAGCTTTTAAAGCCCTCGTACTCTCCCGCTATGCGTGTTACCTGGTAGTACAAAACGGCGACCCTGCGATACCGGTCGTTGCGGCAGGGGAGACtt                  >  1:110680/2‑150 (MQ=255)
                                                                                                                                     ttgaCCATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACtt                  >  1:964958/2‑150 (MQ=255)
                                                                                                                                     ttgaCCATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACtt                  >  2:67185/2‑150 (MQ=255)
                                                                                                                                      tgaCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTa                 >  1:1076141/1‑150 (MQ=255)
                                                                                                                                      tgaCCATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCGATGCCTGTTACATGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTTCGGCAGGGCAGaatga                 >  1:294262/1‑146 (MQ=255)
                                                                                                                                         ccATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAACCGGCGACCCTGCGAAACCGGGCATTGCGGCAGGGCAGACTTAttt              >  2:79630/1‑150 (MQ=255)
                                                                                                                                          cATTTCGCGGAGATCCTCGATATGGTCAAAATTTGCTCATATGCCAAAAGAGCATTAAATGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACATAACGTCGACCCTGCGAAACCGGTCTTTGCGGCAGGGCAGACTTAtttt             <  1:762634/150‑1 (MQ=255)
                                                                                                                                          cATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGTCAGGGCAGACTTAtttt             >  2:1608465/1‑150 (MQ=255)
                                                                                                                                          cATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAtttt             <  1:1204289/150‑1 (MQ=255)
                                                                                                                                           aTTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTg            >  2:1418850/1‑150 (MQ=255)
                                                                                                                                           aTTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTg            >  1:1744411/1‑150 (MQ=255)
                                                                                                                                            tttCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCTCTATGCCTGGTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGc           <  1:1638977/150‑1 (MQ=255)
                                                                                                                                            tttCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGc           <  1:1423764/150‑1 (MQ=255)
                                                                                                                                             ttCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCt          <  2:1319098/150‑1 (MQ=255)
                                                                                                                                               cGAGGAGATCCTCGATATGGTCAAAATTGGGTCAAATGCCAAAAGAGCATTTAATGCCTTCGTACTCTCCCGCGTTGCCTGTTACCTGGTAGTTCAAAACGGCGACCCTGCGATACCGGTCATTGCGGCAGGGGAGGCTTTTTTTGGTAt        >  2:130177/1‑150 (MQ=255)
                                                                                                                                                  ggagATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCa     <  2:1702557/150‑1 (MQ=255)
                                                                                                                                                    agaTCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGa   <  1:1309639/150‑1 (MQ=255)
                                                                                                                                                     gaTCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGAc  >  2:1839980/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTAATGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGAC  >  NC_000913/3817799‑3818097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: