Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,817,957 T→A 7.7% D66E (GAT→GAA dinD → DNA damage‑inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,817,9570TA 7.7% 294.1 / 8.7 108D66E (GAT→GAAdinDDNA damage‑inducible protein
Reads supporting (aligned to +/- strand):  ref base T (49/50);  new base A (7/2);  total (56/52)
Fisher's exact test for biased strand distribution p-value = 1.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

ACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGACCCGACGGC  >  NC_000913/3817809‑3818105
                                                                                                                                                    |                                                                                                                                                    
aCGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGata                                                                                                                                                     >  2:1658591/1‑150 (MQ=255)
 cGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGatat                                                                                                                                                    <  2:1149905/150‑1 (MQ=255)
   gAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCATAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATgg                                                                                                                                                  <  2:634753/150‑1 (MQ=255)
   gAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATgg                                                                                                                                                  <  2:591978/150‑1 (MQ=255)
     ccAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATATCGAGGATCTCCTCGAAATGGTc                                                                                                                                                <  1:656744/149‑1 (MQ=255)
       aGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTTTCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACGATTGATGACCATTTCGAGGAGATCCTCGATATGGTCaa                                                                                                                                              >  1:944685/1‑150 (MQ=255)
       aGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACATTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCaa                                                                                                                                              >  2:404739/1‑150 (MQ=255)
       aGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCaa                                                                                                                                              <  2:204301/150‑1 (MQ=255)
             aTGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTgg                                                                                                                                        <  1:723035/150‑1 (MQ=255)
              tGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGc                                                                                                                                       <  2:64954/150‑1 (MQ=255)
              tGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGc                                                                                                                                       <  1:1027164/150‑1 (MQ=255)
                ggcAGCAAGACAAATTGGGAAACAAATTGGTTATTCATAGGATCGTGACTTTATAACTTTATTAAAGAGCGCCCAAGAAGCCTGTGAGAACAGTCGTCACACAATTGATGACCATTTAGAGGAGATCCTCGAAATGGTCAAAATTTGCta                                                                                                                                     >  2:931530/3‑149 (MQ=255)
                  cAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCaa                                                                                                                                   >  2:1309639/1‑150 (MQ=255)
                   aGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCaaa                                                                                                                                  <  1:1716038/150‑1 (MQ=255)
                       aGACAACTTGCCAAAATACTGGTATATACATAGTATCGTCACTTTATACCTGTATTTACGCCCCCCCACGAAGCCTGTGAGAACAGTTGTCACACATATGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGcc                                                                                                                              <  2:1746464/150‑1 (MQ=255)
                            aCTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAag                                                                                                                         >  2:1547960/1‑150 (MQ=255)
                                gggAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCa                                                                                                                     >  1:1319098/1‑150 (MQ=255)
                                gggAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCa                                                                                                                     <  1:741706/150‑1 (MQ=255)
                                 ggAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCAt                                                                                                                    <  2:545610/150‑1 (MQ=255)
                                  gAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCAtt                                                                                                                   <  2:1302047/150‑1 (MQ=255)
                                      ctactGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTaaaa                                                                                                               <  1:1176035/150‑1 (MQ=255)
                                      ctactGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTaaaa                                                                                                               >  1:952444/1‑150 (MQ=255)
                                         ctGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGAc                                                                                                            <  2:868525/150‑1 (MQ=255)
                                           gggTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACAt                                                                                                          >  1:1074178/1‑150 (MQ=255)
                                             gTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATct                                                                                                        >  1:229388/1‑149 (MQ=255)
                                                aTTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAATAAGCCTGTGAGAAAAGTGGTCACACAATTGATGCCCATTTCGCGGAGCTCCTCGATATGGTCAAAATTGGCTCAAATGACAAAAGAGCATTAAAAGACATCGTAc                                                                                                     >  1:308993/1‑150 (MQ=255)
                                                  tCAGAGTATCCTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGAGAGAACAGTGGTCAAACAATTGATGACCATTTCGAGGAGATACTCGAAATCGTCAAAATTGGCTCAAAACCCAAAAGAGCATTAAAAGACATCGTActa                                                                                                   >  2:762634/1‑149 (MQ=255)
                                                      tgTATCGTCACTTTATACCTGTATTAACGCGCGCCTAAGAAGCCTGTGAGAACAGTGGTCACACATTTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTccc                                                                                               <  2:565563/149‑1 (MQ=255)
                                                        tATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGc                                                                                             <  2:423635/150‑1 (MQ=255)
                                                        tATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGc                                                                                             <  1:1446565/150‑1 (MQ=255)
                                                         aTCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCt                                                                                            >  1:1269623/1‑150 (MQ=255)
                                                         aTCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCt                                                                                            <  1:1194454/150‑1 (MQ=255)
                                                         aTCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACAATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGACAAAAGAGCATTAAAAGACATCGTACTCTCCCGCt                                                                                            >  1:1650466/1‑150 (MQ=255)
                                                          tCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTa                                                                                           <  1:1658591/150‑1 (MQ=255)
                                                           cGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTTATGACCAATTCGAGGAGATCCTCGATATGGTCAAAATAGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTAt                                                                                          >  2:792811/1‑150 (MQ=255)
                                                           cGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTAt                                                                                          >  2:960919/1‑150 (MQ=255)
                                                             tCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGc                                                                                        <  1:1697618/150‑1 (MQ=255)
                                                               aCTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACGATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCt                                                                                      <  2:944685/150‑1 (MQ=255)
                                                               aCTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCAATTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCt                                                                                      <  2:289024/150‑1 (MQ=255)
                                                                cTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTg                                                                                     <  2:1277297/150‑1 (MQ=255)
                                                                    ataCCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTAGATATGGTCAAAATTGGCTCAAATGCAAAAAGAGCATTAAAAGCCATCGTACTCTCCCGCTATGCCTGTGAc                                                                                 >  1:1681405/1‑150 (MQ=255)
                                                                     taCCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTAcc                                                                                >  1:978129/1‑150 (MQ=255)
                                                                       ccTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAATACATCGTACTCTCCCGCTATGCCTGTTACCTg                                                                              >  1:245528/1‑150 (MQ=255)
                                                                       ccTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTg                                                                              >  1:880775/1‑150 (MQ=255)
                                                                        cTGTATTAACGCGCGCCAAAGAAGCCTGTGATAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTgg                                                                             <  2:1131471/150‑1 (MQ=255)
                                                                            aTTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgtagt                                                                         <  2:847746/150‑1 (MQ=255)
                                                                            aTTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgtagt                                                                         >  1:1451921/1‑150 (MQ=255)
                                                                              tAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTAc                                                                       <  2:1519735/150‑1 (MQ=255)
                                                                               aaCGCGCGCCAAAGAAGCCTTTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACa                                                                      <  2:1547667/150‑1 (MQ=255)
                                                                                     cgcCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCACATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACgg                                                                >  2:1671436/1‑150 (MQ=255)
                                                                                      tctgaagaagCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGTTAGTACAAAACGGc                                                               >  1:139419/5‑150 (MQ=255)
                                                                                      gcCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGc                                                               <  1:49020/150‑1 (MQ=255)
                                                                                      gcCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCGCGATATGGTCAAAATTGGCTCAAATGCCAAAAGCGCATTAAAAGCCATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGc                                                               >  1:491234/1‑150 (MQ=255)
                                                                                       ccAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCg                                                              >  1:186456/1‑150 (MQ=255)
                                                                                         aaaGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGAc                                                            <  1:484295/150‑1 (MQ=255)
                                                                                          aagaagCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGAcc                                                           <  2:1241494/150‑1 (MQ=255)
                                                                                           agaagCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGAccc                                                          >  2:64045/1‑150 (MQ=255)
                                                                                            gaagCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCTTTAAAAGCCATCGTACTCTCCCGCTATGCATGTTACCTGGTTGTACAAAACGGCGCCCCt                                                         >  2:302764/1‑150 (MQ=255)
                                                                                              agCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGc                                                       >  1:1361459/1‑150 (MQ=255)
                                                                                              agCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGc                                                       <  1:1547960/150‑1 (MQ=255)
                                                                                                      agaACAGTTGTCACACAATTGATGAGCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCGTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCgg                                               <  1:303642/150‑1 (MQ=255)
                                                                                                       gaACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGt                                              >  1:1752998/1‑150 (MQ=255)
                                                                                                         aCAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCa                                            >  2:1692282/1‑150 (MQ=255)
                                                                                                         aCAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCa                                            >  2:1349411/1‑150 (MQ=255)
                                                                                                          cAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCAt                                           <  1:1084669/150‑1 (MQ=255)
                                                                                                           aGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCAtt                                          >  2:525634/1‑150 (MQ=255)
                                                                                                             gggTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGc                                        <  2:708530/149‑1 (MQ=255)
                                                                                                               gTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCgg                                      >  1:1658539/1‑150 (MQ=255)
                                                                                                                acacacaATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACTGCGACCCTTCGAAACCGGTCATTGCggc                                     <  1:90004/149‑1 (MQ=255)
                                                                                                                  acacaATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAAATTGATCAAATCACAAAAGAGCATTAAAACAAAAAATACTATCCCGCTATGCCTGTTACCTGGTAGTACAAAAAGGCGACCCTGCGAAACCGGGAATTGCggcag                                   >  2:1086326/1‑150 (MQ=255)
                                                                                                                   cacaATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGCCCCTGCGAAACCGGTCATTGCggcagg                                  >  2:1783600/1‑150 (MQ=255)
                                                                                                                    acaATGGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACAGCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcaggg                                 <  1:1188046/150‑1 (MQ=255)
                                                                                                                     caATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcagggc                                <  2:1043519/150‑1 (MQ=255)
                                                                                                                     caATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTATCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTCCGAAACCGGTAATTGCggcagggc                                >  2:1411857/1‑150 (MQ=255)
                                                                                                                     aaaTTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAGGAGCATAAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGAAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcagggc                                <  1:633650/149‑1 (MQ=255)
                                                                                                                      aaTTGATGACCATTTCGAGGAGATCCTCGATATTGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcagggca                               <  1:839248/150‑1 (MQ=255)
                                                                                                                         tgatgaCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGAc                            <  1:742574/150‑1 (MQ=255)
                                                                                                                          gatgaCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACt                           >  2:1700334/1‑150 (MQ=255)
                                                                                                                          gatgaCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACAGCGTACTCTACCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACGCTGCGAACCCGGTCATTGCGGCAGCGCAGACt                           >  2:1121087/1‑150 (MQ=255)
                                                                                                                           ttgaCCATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAGAAGAGCTTTTAAAGCCCTCGTACTCTCCCGCTATGCGTGTTACCTGGTAGTACAAAACGGCGACCCTGCGATACCGGTCGTTGCGGCAGGGGAGACtt                          >  1:110680/2‑150 (MQ=255)
                                                                                                                           ttgaCCATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACtt                          >  1:964958/2‑150 (MQ=255)
                                                                                                                           ttgaCCATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACtt                          >  2:67185/2‑150 (MQ=255)
                                                                                                                            tgaCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTa                         >  1:1076141/1‑150 (MQ=255)
                                                                                                                            tgaCCATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCGATGCCTGTTACATGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTTCGGCAGGGCAGaatga                         >  1:294262/1‑146 (MQ=255)
                                                                                                                             gaCCATATCGAGGATCTCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACTTCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAt                        >  2:1750548/1‑150 (MQ=255)
                                                                                                                               ccATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAACCGGCGACCCTGCGAAACCGGGCATTGCGGCAGGGCAGACTTAttt                      >  2:79630/1‑150 (MQ=255)
                                                                                                                                cATTTCGCGGAGATCCTCGATATGGTCAAAATTTGCTCATATGCCAAAAGAGCATTAAATGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACATAACGTCGACCCTGCGAAACCGGTCTTTGCGGCAGGGCAGACTTAtttt                     <  1:762634/150‑1 (MQ=255)
                                                                                                                                cATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGTCAGGGCAGACTTAtttt                     >  2:1608465/1‑150 (MQ=255)
                                                                                                                                cATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAtttt                     <  1:1204289/150‑1 (MQ=255)
                                                                                                                                 aTTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTg                    >  1:1744411/1‑150 (MQ=255)
                                                                                                                                  tttCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCTCTATGCCTGGTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGc                   <  1:1638977/150‑1 (MQ=255)
                                                                                                                                  tttCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGc                   <  1:1423764/150‑1 (MQ=255)
                                                                                                                                   ttCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCt                  <  2:1319098/150‑1 (MQ=255)
                                                                                                                                     cGAGGAGATCCTCGATATGGTCAAAATTGGGTCAAATGCCAAAAGAGCATTTAATGCCTTCGTACTCTCCCGCGTTGCCTGTTACCTGGTAGTTCAAAACGGCGACCCTGCGATACCGGTCATTGCGGCAGGGGAGGCTTTTTTTGGTAt                >  2:130177/1‑150 (MQ=255)
                                                                                                                                        ggagATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCa             <  2:1702557/150‑1 (MQ=255)
                                                                                                                                          agaTCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGa           <  1:1309639/150‑1 (MQ=255)
                                                                                                                                          agaTCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGa           >  1:1518469/1‑150 (MQ=255)
                                                                                                                                           gaTCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGAc          >  2:1839980/1‑150 (MQ=255)
                                                                                                                                             tCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGAccc        >  2:348771/1‑150 (MQ=255)
                                                                                                                                                   atatGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGACCCGACGGc  >  2:1042743/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGACCCGACGGC  >  NC_000913/3817809‑3818105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: