Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,874,049 G→C 6.3% C142W (TGC→TGG) ‡ dgoK ← 2‑oxo‑3‑deoxygalactonate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,874,0490GC 6.3% 418.1 / 18.5 143C142W (TGC→TGG) ‡dgoK2‑oxo‑3‑deoxygalactonate kinase
Reads supporting (aligned to +/- strand):  ref base G (59/74);  new base C (3/6);  total (62/81)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.60e-02

Only 100 of 144 total aligned reads displayed.

TCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAACTGACG  >  NC_000913/3873900‑3874197
                                                                                                                                                     |                                                                                                                                                    
 cAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGc                                                                                                                                                     <  2:997286/150‑1 (MQ=255)
 cAAGGACAGCTGTGAAGGCATCGGCAGAGGTTTCCTGCGGCGGCAAACCTGTGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGc                                                                                                                                                     <  1:39473/150‑1 (MQ=255)
   aGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCaa                                                                                                                                                   <  1:1062965/150‑1 (MQ=255)
     gCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATg                                                                                                                                                 >  1:761428/1‑150 (MQ=255)
       cAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATggg                                                                                                                                               >  2:503811/1‑150 (MQ=255)
       cAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATggg                                                                                                                                               >  2:661448/1‑150 (MQ=255)
       cAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTGCAAATggg                                                                                                                                               <  1:1378230/150‑1 (MQ=255)
       cAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATATAACAGTAAATGATGAAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCGGCACCCATTGCAAATggg                                                                                                                                               <  1:1414047/150‑1 (MQ=255)
        aGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTGCAAATGGGt                                                                                                                                              <  1:33578/150‑1 (MQ=255)
           tgtgAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTcc                                                                                                                                           >  2:1113609/1‑150 (MQ=255)
            gtgAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCg                                                                                                                                          >  1:1557710/1‑150 (MQ=255)
             tgAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCgg                                                                                                                                         >  2:204911/1‑150 (MQ=255)
              gAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACGGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCggg                                                                                                                                        <  1:201956/150‑1 (MQ=255)
              gAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCggg                                                                                                                                        >  1:1351404/1‑150 (MQ=255)
              gAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGAATTGCTGGCTATCGGCCTGCACCCATTGCAAATGGGtgcaggc                                                                                                                                        <  1:1448128/150‑7 (MQ=255)
               aaGGCTTCTGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGc                                                                                                                                       <  1:1639556/150‑1 (MQ=255)
               aaGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGc                                                                                                                                       >  1:117015/1‑150 (MQ=255)
                aGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCa                                                                                                                                      <  2:836730/150‑1 (MQ=255)
                   cATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGa                                                                                                                                   >  1:213708/1‑150 (MQ=255)
                   cATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGa                                                                                                                                   <  1:1190688/150‑1 (MQ=255)
                   cATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGa                                                                                                                                   >  1:1126840/1‑150 (MQ=255)
                   cATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGa                                                                                                                                   <  1:619249/150‑1 (MQ=255)
                    aTCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGAc                                                                                                                                  <  1:325651/150‑1 (MQ=255)
                          agagTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAag                                                                                                                            <  1:1320447/150‑1 (MQ=255)
                           gagTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAaga                                                                                                                           <  2:1477233/150‑1 (MQ=255)
                           gagTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAaga                                                                                                                           >  1:233137/1‑150 (MQ=255)
                              ttttCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAagagag                                                                                                                        <  2:932612/150‑1 (MQ=255)
                               tttCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGagg                                                                                                                       >  2:223302/1‑150 (MQ=255)
                               tttCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGagg                                                                                                                       <  2:926556/150‑1 (MQ=255)
                                ttCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACTGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGagga                                                                                                                      <  2:536703/150‑1 (MQ=255)
                                      cggcggCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGc                                                                                                                >  1:1612937/1‑150 (MQ=255)
                                         cggcAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCag                                                                                                             >  1:675885/1‑150 (MQ=255)
                                           gcAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCagag                                                                                                           >  2:554820/1‑150 (MQ=255)
                                            cAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGc                                                                                                          >  2:613497/1‑150 (MQ=255)
                                                  tGCGCCAATCAATGAGTGATTTAACAGTAATTGATTTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTAGCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTcgcgc                                                                                                    <  1:158581/150‑1 (MQ=255)
                                                     gcCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGcc                                                                                                 >  1:1392945/1‑150 (MQ=255)
                                                      ccAATCAATGTTTGATTTAACAGTAAATTATGTAAATCACCGGTCATCACGGTGCTAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAACAGAGGACGGAGCCAGAGCTCGCGCGCCg                                                                                                <  2:1428256/150‑1 (MQ=255)
                                                      ccAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCg                                                                                                <  1:1114010/150‑1 (MQ=255)
                                                         atctatGAGTGATTTAACAGTAAATGATGTAATTCTCCGGTAATGACGGTGCGAAACTCGGTGATTTGCTGGCTATCGGGCTGCACCAATTTGCAATGGGTTCCGGGCAGGACATAAAGTGAGGATGGAGCCAGAGCTCGCGCGCCGATc                                                                                             <  1:65402/150‑1 (MQ=255)
                                                         atcaatGAGTGATTTAACAGTAAATGATGTAATTCCCCGGTCATCACGGTGCGAAACGCGTTGAGTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGCGAGGAAGGAGCCAGAGCTCGCGCGCCGATc                                                                                             <  1:1704214/150‑1 (MQ=255)
                                                         atcaatGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATc                                                                                             >  1:1867948/1‑150 (MQ=255)
                                                            aatGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGGAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCTGAGCTCGCGCGCCGCTCAAt                                                                                          >  2:1139614/1‑150 (MQ=255)
                                                            aatGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAAt                                                                                          >  2:980660/1‑150 (MQ=255)
                                                                aGTGTTTTAACAGTAAATGAGGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATTGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATtgtg                                                                                      <  1:770686/150‑1 (MQ=255)
                                                                aGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATtgtg                                                                                      <  2:1685358/150‑1 (MQ=255)
                                                                 gTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTgtgt                                                                                     >  1:1287576/1‑150 (MQ=255)
                                                                  tGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTCTCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGtt                                                                                    <  2:1869982/150‑1 (MQ=255)
                                                                   gATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGttt                                                                                   >  1:1855676/1‑150 (MQ=255)
                                                                           tgTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGAGATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGAGCAATTGTGTTTCTTcgccg                                                                           >  2:185671/2‑150 (MQ=255)
                                                                           aGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTAATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTcgccg                                                                           <  1:686792/150‑1 (MQ=255)
                                                                               aaTGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCgcgcc                                                                       >  1:1876078/1‑149 (MQ=255)
                                                                               aaTGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCa                                                                       <  1:974626/150‑1 (MQ=255)
                                                                                 ttatgTAATTCACCGGTCATCACGTTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGAATTACATAAAGAGACGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATc                                                                     <  2:1623705/148‑1 (MQ=255)
                                                                                 tgatgTAATGCTCCGGTCATCACGGTGCGCAACTCGTGGTTTTGCTGGCTCTCTGCCTGCACCCATATGCCATGGGTTCCGGGCATGTCCTAAAGCTAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTGTCTTCGCAGCGCATc                                                                     <  2:1623704/150‑1 (MQ=255)
                                                                                     gTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACAt                                                                 <  1:797073/150‑1 (MQ=255)
                                                                                         ttCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATtgtg                                                             >  1:1829927/1‑150 (MQ=255)
                                                                                           cACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGt                                                           >  2:612638/1‑150 (MQ=255)
                                                                                               ggTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCTGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAATGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCAGCGCATCACATTGTGGTTATc                                                       >  1:259532/1‑150 (MQ=255)
                                                                                               ggTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCGGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATc                                                       <  2:1067323/150‑1 (MQ=255)
                                                                                                 tcatcaCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGt                                                     <  1:1555293/150‑1 (MQ=255)
                                                                                                 tcatcaCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGt                                                     <  1:1630605/150‑1 (MQ=255)
                                                                                                   atcaCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGTAGCCAGAGCTCGCGCGCAGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCa                                                   <  1:126657/150‑1 (MQ=255)
                                                                                                   atcaCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCa                                                   <  2:1044208/150‑1 (MQ=255)
                                                                                                    tcaCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCAt                                                  >  1:538752/1‑150 (MQ=255)
                                                                                                     caCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATg                                                 <  2:1832414/150‑1 (MQ=255)
                                                                                                     caCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATg                                                 <  2:117015/150‑1 (MQ=255)
                                                                                                     caCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATg                                                 >  1:616069/1‑150 (MQ=255)
                                                                                                       cGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATATGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATgag                                               <  2:1869059/150‑1 (MQ=255)
                                                                                                          tGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACACATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATgagaga                                            >  2:545416/1‑150 (MQ=255)
                                                                                                            cGAAAATCGGTGATTTGCTCGGTTTCGGCCTGCACGCTTTTGCCTTGGGTTCCGGGCATGACATAAAGACCGGAAGGAGCCAGAGCTCGCGCGCCGATCATTTGTGTTGCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGaca                                          <  2:364495/150‑1 (MQ=255)
                                                                                                            cGAAAACCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGaca                                          >  1:326493/1‑150 (MQ=255)
                                                                                                              aaaaTCTTTGATTTGCTGGCTATCGGACTGCACCAATTTGCAATGGGTTCCGGGCATGACATATAGTGCGGAAGGAGCAAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCTTGAGAGAcaca                                        <  1:1147206/150‑1 (MQ=255)
                                                                                                               aaaTCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACAt                                       >  2:1381064/1‑150 (MQ=255)
                                                                                                                    gTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATcc                                  >  2:1505135/1‑150 (MQ=255)
                                                                                                                      tGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCgg                                <  2:233137/150‑1 (MQ=255)
                                                                                                                       gagttGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCggg                               <  2:1832522/147‑1 (MQ=255)
                                                                                                                       gATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCggg                               <  1:1415226/150‑1 (MQ=255)
                                                                                                                             cTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGTGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGaataat                         <  1:4160/150‑1 (MQ=255)
                                                                                                                              tGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATc                        <  2:1351404/150‑1 (MQ=255)
                                                                                                                              tGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATc                        <  1:653012/150‑1 (MQ=255)
                                                                                                                                 cacgcGGCCTGCAGCAATTTGCAATGCGTTCCGGGCATGACATACAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCTATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTAGCGGCATGAGAGACACATAATCCGGGAATAATCCAg                     <  2:323678/146‑1 (MQ=255)
                                                                                                                                 cTATCGGCCTGCACGCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAg                     <  2:1576002/150‑1 (MQ=255)
                                                                                                                                 cTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCCCTTTGTGGTTATCGTCATGAGAGACACATCATCCGGGAATAATCCAg                     >  1:140748/1‑150 (MQ=255)
                                                                                                                                 cTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAg                     <  2:538752/150‑1 (MQ=255)
                                                                                                                                 cTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAg                     <  2:1208506/150‑1 (MQ=255)
                                                                                                                                 cTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAg                     >  2:1772438/1‑150 (MQ=255)
                                                                                                                                 cTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAg                     <  2:1568557/150‑1 (MQ=255)
                                                                                                                                  tATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGa                    <  1:110569/150‑1 (MQ=255)
                                                                                                                                   aTCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGat                   >  1:1502680/1‑150 (MQ=255)
                                                                                                                                      ggCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGGCGCTCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATAtt                >  2:298646/1‑150 (MQ=255)
                                                                                                                                         cggaaCCCATTGCAAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTc             >  1:1641032/5‑150 (MQ=255)
                                                                                                                                         cggaaCCCATTGCAAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTc             >  1:1295522/5‑150 (MQ=255)
                                                                                                                                           gCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGc           >  2:1875290/1‑150 (MQ=255)
                                                                                                                                           gCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGc           >  2:834270/1‑150 (MQ=255)
                                                                                                                                           gCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGc           <  1:1351846/150‑1 (MQ=255)
                                                                                                                                            cACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGcc          >  1:578913/1‑150 (MQ=255)
                                                                                                                                              cccATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCaa        >  1:1317581/1‑150 (MQ=255)
                                                                                                                                                cATTTGCAATGGGTTCCGGGAATGACATAAAGAGAGGAAGGAGCCAGAGCTCTCTCGACGATCAATTGAGTTTCTTCGCCTCGCATCACATTGTGGTTATCGTCATGAGACACACATAATCCCGGAATAATACAGATATTGTCGCCAACt      >  2:472721/1‑150 (MQ=255)
                                                                                                                                                  tttGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAACTGa    <  1:980660/150‑1 (MQ=255)
                                                                                                                                                   ttGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCATCTGAc   <  2:1557710/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAACTGACG  >  NC_000913/3873900‑3874197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: