Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,874,050 C→A 6.3% C142F (TGC→TTC) ‡ dgoK ← 2‑oxo‑3‑deoxygalactonate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,874,0500CA 6.3% 374.9 / 13.6 140C142F (TGC→TTC) ‡dgoK2‑oxo‑3‑deoxygalactonate kinase
Reads supporting (aligned to +/- strand):  ref base C (58/72);  new base A (3/6);  total (62/78)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-01

Only 100 of 145 total aligned reads displayed.

CAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAACTGACGTT  >  NC_000913/3873901‑3874199
                                                                                                                                                     |                                                                                                                                                     
  aGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCaa                                                                                                                                                     <  1:1062965/150‑1 (MQ=255)
  aGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCaa                                                                                                                                                     >  1:400058/1‑150 (MQ=255)
    gCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATg                                                                                                                                                   >  1:761428/1‑150 (MQ=255)
      cAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTGCAAATggg                                                                                                                                                 <  1:1378230/150‑1 (MQ=255)
      cAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATATAACAGTAAATGATGAAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCGGCACCCATTGCAAATggg                                                                                                                                                 <  1:1414047/150‑1 (MQ=255)
       aGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTGCAAATGGGt                                                                                                                                                <  1:33578/150‑1 (MQ=255)
           gtgAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCg                                                                                                                                            >  1:1557710/1‑150 (MQ=255)
            tgAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAAAGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTGCAAATGGGtgcagg                                                                                                                                           <  2:970827/150‑6 (MQ=255)
             gAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACGGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCggg                                                                                                                                          <  1:201956/150‑1 (MQ=255)
             gAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCggg                                                                                                                                          >  1:1351404/1‑150 (MQ=255)
             gAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGAATTGCTGGCTATCGGCCTGCACCCATTGCAAATGGGtgcaggc                                                                                                                                          <  1:1448128/150‑7 (MQ=255)
              aaGGCTTCTGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGc                                                                                                                                         <  1:1639556/150‑1 (MQ=255)
              aaGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGc                                                                                                                                         >  1:117015/1‑150 (MQ=255)
               aGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCa                                                                                                                                        >  1:1303297/1‑150 (MQ=255)
                  cATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGa                                                                                                                                     <  1:1190688/150‑1 (MQ=255)
                  cATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGa                                                                                                                                     >  1:1126840/1‑150 (MQ=255)
                   aTCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGAc                                                                                                                                    <  1:325651/150‑1 (MQ=255)
                         agagTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAag                                                                                                                              <  1:1320447/150‑1 (MQ=255)
                          gagTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAaga                                                                                                                             >  1:233137/1‑150 (MQ=255)
                          gagTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAaga                                                                                                                             <  2:1477233/150‑1 (MQ=255)
                             ttttCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAagagag                                                                                                                          <  2:1873487/150‑1 (MQ=255)
                              tttCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGagg                                                                                                                         >  2:223302/1‑150 (MQ=255)
                              tttCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGagg                                                                                                                         <  2:926556/150‑1 (MQ=255)
                               ttCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACTGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGagga                                                                                                                        <  2:536703/150‑1 (MQ=255)
                               ttCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGagga                                                                                                                        <  2:529332/150‑1 (MQ=255)
                                 ccTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGaggaag                                                                                                                      >  1:625326/1‑150 (MQ=255)
                                 ccTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGaggaag                                                                                                                      >  2:1013736/1‑150 (MQ=255)
                                     cggcggCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGc                                                                                                                  >  1:1612937/1‑150 (MQ=255)
                                        cggcAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCag                                                                                                               >  1:675885/1‑150 (MQ=255)
                                          gcAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCagag                                                                                                             >  2:554820/1‑150 (MQ=255)
                                           cAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGc                                                                                                            >  2:613497/1‑150 (MQ=255)
                                             aaCCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTc                                                                                                          >  2:110569/1‑150 (MQ=255)
                                                 tGCGCCAATCAATGAGTGATTTAACAGTAATTGATTTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTAGCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTcgcgc                                                                                                      <  1:158581/150‑1 (MQ=255)
                                                    gcCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGcc                                                                                                   >  1:1392945/1‑150 (MQ=255)
                                                     ccAATCAATGTTTGATTTAACAGTAAATTATGTAAATCACCGGTCATCACGGTGCTAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAACAGAGGACGGAGCCAGAGCTCGCGCGCCg                                                                                                  <  2:1428256/150‑1 (MQ=255)
                                                     ccAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCg                                                                                                  >  1:1832414/1‑150 (MQ=255)
                                                     ccAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCg                                                                                                  <  1:1114010/150‑1 (MQ=255)
                                                       aatcaatGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGAt                                                                                                <  2:542298/150‑1 (MQ=255)
                                                        atcaatGAGTGATTTAACAGTAAATGATGTAATTCCCCGGTCATCACGGTGCGAAACGCGTTGAGTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGCGAGGAAGGAGCCAGAGCTCGCGCGCCGATc                                                                                               <  1:1704214/150‑1 (MQ=255)
                                                        atcaatGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATc                                                                                               >  1:1867948/1‑150 (MQ=255)
                                                          caatGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCaa                                                                                             >  1:842043/1‑150 (MQ=255)
                                                           aatGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGGAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCTGAGCTCGCGCGCCGCTCAAt                                                                                            >  2:1139614/1‑150 (MQ=255)
                                                           aatGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAAt                                                                                            >  2:980660/1‑150 (MQ=255)
                                                               aGTGTTTTAACAGTAAATGAGGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATTGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATtgtg                                                                                        <  1:770686/150‑1 (MQ=255)
                                                               aGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATtgtg                                                                                        <  2:1685358/150‑1 (MQ=255)
                                                                gTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTgtgt                                                                                       >  1:1287576/1‑150 (MQ=255)
                                                                  gATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGTCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGttt                                                                                     >  1:1436896/1‑150 (MQ=255)
                                                                  gATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGttt                                                                                     >  1:1855676/1‑150 (MQ=255)
                                                                      tAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCCCCCATTTGCAATGGGTACCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGAACAAATGTGTttcgtc                                                                                 >  1:287201/1‑150 (MQ=255)
                                                                          tgTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGAGATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGAGCAATTGTGTTTCTTcgccg                                                                             >  2:185671/2‑150 (MQ=255)
                                                                              aaTGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCgcgcc                                                                         >  1:1876078/1‑149 (MQ=255)
                                                                               atgatgTAATTCACCGGTCATCACGGTGCGAAAATCGTGGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCAt                                                                        <  2:1587296/150‑1 (MQ=255)
                                                                                ttatgTAATTCACCGGTCATCACGTTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGAATTACATAAAGAGACGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATc                                                                       <  2:1623705/148‑1 (MQ=255)
                                                                                tgatgTAATGCTCCGGTCATCACGGTGCGCAACTCGTGGTTTTGCTGGCTCTCTGCCTGCACCCATATGCCATGGGTTCCGGGCATGTCCTAAAGCTAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTGTCTTCGCAGCGCATc                                                                       <  2:1623704/150‑1 (MQ=255)
                                                                                          cACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGt                                                             >  2:612638/1‑150 (MQ=255)
                                                                                          cACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGt                                                             <  1:1553949/150‑1 (MQ=255)
                                                                                              ggTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCGGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATc                                                         <  2:1067323/150‑1 (MQ=255)
                                                                                               gTCAGCACGGTGCGAAAATCGTTGATTTGCTGGCTTTCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCg                                                        <  1:334289/150‑1 (MQ=255)
                                                                                                tcatcaCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGt                                                       <  1:1630605/150‑1 (MQ=255)
                                                                                                tcatcaCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGt                                                       <  1:1555293/150‑1 (MQ=255)
                                                                                                  atcaCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGTAGCCAGAGCTCGCGCGCAGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCa                                                     <  1:126657/150‑1 (MQ=255)
                                                                                                   tcaCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCAt                                                    >  1:538752/1‑150 (MQ=255)
                                                                                                    caCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATg                                                   <  2:1832414/150‑1 (MQ=255)
                                                                                                    caCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATg                                                   >  1:616069/1‑150 (MQ=255)
                                                                                                    caCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATg                                                   <  2:117015/150‑1 (MQ=255)
                                                                                                     aCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATga                                                  <  1:164148/150‑1 (MQ=255)
                                                                                                      cGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATATGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATgag                                                 <  2:1869059/150‑1 (MQ=255)
                                                                                                         tGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACACATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATgagaga                                              >  2:545416/1‑150 (MQ=255)
                                                                                                             aaaaTCTTTGATTTGCTGGCTATCGGACTGCACCAATTTGCAATGGGTTCCGGGCATGACATATAGTGCGGAAGGAGCAAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCTTGAGAGAcaca                                          <  1:1147206/150‑1 (MQ=255)
                                                                                                              aaaTCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACAt                                         >  2:1381064/1‑150 (MQ=255)
                                                                                                                   gTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATcc                                    >  2:1505135/1‑150 (MQ=255)
                                                                                                                     tGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCgg                                  <  2:233137/150‑1 (MQ=255)
                                                                                                                     tGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCgg                                  >  1:749047/1‑150 (MQ=255)
                                                                                                                      gagttGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCggg                                 <  2:1832522/147‑1 (MQ=255)
                                                                                                                      gATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCggg                                 <  2:400058/150‑1 (MQ=255)
                                                                                                                       aTTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGa                                <  2:761428/150‑1 (MQ=255)
                                                                                                                            cTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGTGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGaataat                           <  1:4160/150‑1 (MQ=255)
                                                                                                                             tGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATc                          <  1:653012/150‑1 (MQ=255)
                                                                                                                             tGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATc                          <  2:1351404/150‑1 (MQ=255)
                                                                                                                                cacgcGGCCTGCAGCAATTTGCAATGCGTTCCGGGCATGACATACAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCTATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTAGCGGCATGAGAGACACATAATCCGGGAATAATCCAg                       <  2:323678/146‑1 (MQ=255)
                                                                                                                                cTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCCCTTTGTGGTTATCGTCATGAGAGACACATCATCCGGGAATAATCCAg                       >  1:140748/1‑150 (MQ=255)
                                                                                                                                cTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAg                       >  2:1772438/1‑150 (MQ=255)
                                                                                                                                cTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAg                       <  2:1208506/150‑1 (MQ=255)
                                                                                                                                cTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAg                       <  1:661448/150‑1 (MQ=255)
                                                                                                                                cTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAg                       <  2:1568557/150‑1 (MQ=255)
                                                                                                                                 tATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGa                      <  1:110569/150‑1 (MQ=255)
                                                                                                                                     ggCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGGCGCTCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATAtt                  >  2:298646/1‑150 (MQ=255)
                                                                                                                                         tGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCg              <  1:223302/150‑1 (MQ=255)
                                                                                                                                          gCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGc             <  1:1351846/150‑1 (MQ=255)
                                                                                                                                          gCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGc             >  2:1875290/1‑150 (MQ=255)
                                                                                                                                          gCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGc             >  2:834270/1‑150 (MQ=255)
                                                                                                                                           cACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGTGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGcc            <  2:882582/150‑1 (MQ=255)
                                                                                                                                           cACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGcc            >  1:602846/1‑150 (MQ=255)
                                                                                                                                             cccATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCaa          >  1:1317581/1‑150 (MQ=255)
                                                                                                                                               cATTTGCAATGGGTTCCGGGAATGACATAAAGAGAGGAAGGAGCCAGAGCTCTCTCGACGATCAATTGAGTTTCTTCGCCTCGCATCACATTGTGGTTATCGTCATGAGACACACATAATCCCGGAATAATACAGATATTGTCGCCAACt        >  2:472721/1‑150 (MQ=255)
                                                                                                                                                 tttGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAACTGa      <  1:980660/150‑1 (MQ=255)
                                                                                                                                                  ttGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCATCTGAc     <  2:1557710/150‑1 (MQ=255)
                                                                                                                                                   tGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAACTGACg    <  2:213708/150‑1 (MQ=255)
                                                                                                                                                     cAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAACTGACGtt  >  2:1352712/1‑150 (MQ=255)
                                                                                                                                                     cAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAACTGACGtt  >  1:926618/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAACTGACGTT  >  NC_000913/3873901‑3874199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: