Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,891,506 C→G 5.8% intergenic (+23/‑109) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,891,5060CG 5.8% 422.9 / 12.3 134intergenic (+23/‑109)tnaB/mdtLtryptophan transporter of low affinity/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (64/62);  new base G (5/3);  total (69/65)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

Only 100 of 135 total aligned reads displayed.

TCCCAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGT  >  NC_000913/3891360‑3891649
                                                                                                                                                  |                                                                                                                                               
tCCCTATCAGATGTTCAGGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCGCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCt                                                                                                                                              <  2:1009081/150‑1 (MQ=255)
tCCCAATGAGATGGTGTCGGGCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTTTTACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTATCGTGTTTCCTAATTTTGGCTAAATCCTTCTAGAAGCCAGCCATTCGCt                                                                                                                                              <  2:1055913/150‑1 (MQ=255)
 cccAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAATAAGCCAGCCATTCGCTg                                                                                                                                             >  2:1524577/1‑150 (MQ=255)
  ccAATCAGCTGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTgg                                                                                                                                            <  2:534437/150‑1 (MQ=255)
  ccAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTCTCATTCTCGTAGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTTCCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTgg                                                                                                                                            >  1:1159476/1‑150 (MQ=255)
   cAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGc                                                                                                                                           <  1:1612912/150‑1 (MQ=255)
   cAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGc                                                                                                                                           >  2:683572/1‑150 (MQ=255)
    aaTCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATggctggct                                                                                                                                          <  1:1745722/150‑1 (MQ=255)
     aTCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGAGTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGCAGCCAGCCATTCGCTGGctt                                                                                                                                         >  2:1602569/1‑150 (MQ=255)
        cgATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGcttctt                                                                                                                                      <  2:873603/149‑1 (MQ=255)
        aGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGcttctt                                                                                                                                      >  2:55624/1‑150 (MQ=255)
         gATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTg                                                                                                                                     <  2:874964/150‑1 (MQ=255)
         gATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGATTCTTg                                                                                                                                     >  1:36650/1‑150 (MQ=255)
         gATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTGCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGCTTCTTg                                                                                                                                     <  1:1062153/150‑1 (MQ=255)
          aTGTTCACGGTCGGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGc                                                                                                                                    <  2:78476/150‑1 (MQ=255)
           tGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGcc                                                                                                                                   <  2:1800203/150‑1 (MQ=255)
            gTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCct                                                                                                                                  >  2:1257916/1‑150 (MQ=255)
            gTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGAATGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGCTTCTTgaag                                                                                                                                  <  1:1631526/150‑4 (MQ=255)
            gTTCACGGGCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTGCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCct                                                                                                                                  <  2:1106975/150‑1 (MQ=255)
              tCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTCCCTAAATTTGGCTAAATCCTACAAGAAGACAGCCATTCGCTGGCTTCTTGCctcg                                                                                                                                >  2:885234/1‑149 (MQ=255)
              tCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCctct                                                                                                                                >  1:199620/1‑150 (MQ=255)
              tCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTAAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCctct                                                                                                                                >  1:14164/1‑150 (MQ=255)
                 cGGTCTGGGGCGGCAATCATATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTATAACGTGTTACATAAATTTGGCTTAAACCTTCAAGAAGCCAGACATTCGCTGGCTTCTTGCCTCTCat                                                                                                                             >  2:1463638/1‑149 (MQ=255)
                  ggTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAgg                                                                                                                            <  1:1226364/150‑1 (MQ=255)
                  ggTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAgg                                                                                                                            <  2:827761/150‑1 (MQ=255)
                    tCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGaa                                                                                                                          >  1:1656508/1‑150 (MQ=255)
                       ggggCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATc                                                                                                                       <  1:828246/150‑1 (MQ=255)
                         ggcggcAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCAc                                                                                                                     <  1:1390736/150‑1 (MQ=255)
                           cggcAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACtt                                                                                                                   >  1:852177/1‑150 (MQ=255)
                            ggcAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTa                                                                                                                  >  1:1662778/1‑150 (MQ=255)
                             gcAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTAt                                                                                                                 <  1:1663195/150‑1 (MQ=255)
                             gcAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTAAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTAt                                                                                                                 >  1:843420/1‑150 (MQ=255)
                             gcAATCTTAATCCGGCGATTGTCATTCTCTTTGGTATAACCGTGAATTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTAt                                                                                                                 >  1:1766039/1‑150 (MQ=255)
                              cAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATg                                                                                                                <  2:576555/150‑1 (MQ=255)
                                aTCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTc                                                                                                              <  2:604153/150‑1 (MQ=255)
                                  cTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCa                                                                                                            <  1:860793/150‑1 (MQ=255)
                                     aTTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAAt                                                                                                         >  2:430756/1‑150 (MQ=255)
                                      taccGGCGATTCTCAATCTCTTTGCTATATCCGTGATTTTGTGATGGTTCGGCAACGTCTTTAACGGGTTACCTAACTTTGGCTAAATCCTTCAAGAAGCCAGCCATTGGCTGGCATCTAGCCTCTCAGGAAATCACATATGTACAAATg                                                                                                        >  2:404481/3‑150 (MQ=255)
                                            cGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACt                                                                                                  >  1:673929/1‑150 (MQ=255)
                                             gATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTc                                                                                                 <  1:965563/150‑1 (MQ=255)
                                                tGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAGATCACTTATGTCCAAATGGCAACTCGcc                                                                                              <  2:377570/150‑1 (MQ=255)
                                                tGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGcc                                                                                              >  1:1841278/1‑150 (MQ=255)
                                                 gTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTAGCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCAGGCTTCTTGCCTCTCAGGAAATCACGTATGTCCAAATGGCCACTCGCCt                                                                                             >  2:1777563/1‑150 (MQ=255)
                                                   cATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGa                                                                                           <  2:502939/150‑1 (MQ=255)
                                                    aTTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAt                                                                                          <  1:618362/150‑1 (MQ=255)
                                                    aTTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAt                                                                                          >  2:1663978/1‑150 (MQ=255)
                                                     ttCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtc                                                                                         <  2:624973/150‑1 (MQ=255)
                                                      tctcTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcc                                                                                        <  1:1090365/150‑1 (MQ=255)
                                                             ggTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTca                                                                                 >  1:1006910/1‑150 (MQ=255)
                                                             ggTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGCGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTca                                                                                 <  2:1608707/150‑1 (MQ=255)
                                                              gTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTcac                                                                                >  1:703448/1‑150 (MQ=255)
                                                                ataACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACATAAATTTGGATAAATCCTTCAAGAAGCCAGACATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATACTCCTTcacca                                                                              >  1:135335/1‑150 (MQ=255)
                                                                 taACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGACAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGTCAACTCGCCTGATCCTCCTTcaccac                                                                             >  1:1562928/1‑150 (MQ=255)
                                                                  aaCCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACg                                                                            >  1:754680/1‑150 (MQ=255)
                                                                  aaCCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACg                                                                            <  1:473727/150‑1 (MQ=255)
                                                                    ccGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCAATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTa                                                                          >  1:124893/1‑150 (MQ=255)
                                                                     cGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTAt                                                                         >  2:1046078/1‑150 (MQ=255)
                                                                     cGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATATCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTAt                                                                         >  2:621043/1‑150 (MQ=255)
                                                                         aTTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCtt                                                                     >  2:979377/1‑150 (MQ=255)
                                                                             tgtgCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCg                                                                 <  1:982286/150‑1 (MQ=255)
                                                                             tgtgCGGGGTCGGCTACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTTGCCTGATCCTCCTTCACCACGTATGCTTTGCg                                                                 <  1:1014049/150‑1 (MQ=255)
                                                                               tgCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTc                                                               <  2:1860247/150‑1 (MQ=255)
                                                                               ggCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTc                                                               <  2:961792/149‑1 (MQ=255)
                                                                                gCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCa                                                              <  1:1831627/150‑1 (MQ=255)
                                                                                    gTTCGGCAACGTCTTTAACGTGTTTCCTAAATTTGGCTAAATCCTTCAAGAAGGCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCtt                                                          <  2:25442/150‑1 (MQ=255)
                                                                                       cGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGAGTCACCTTACt                                                       >  2:299678/1‑150 (MQ=255)
                                                                                          cAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATc                                                    <  1:163286/150‑1 (MQ=255)
                                                                                           aaCGTCTTTAACGTGTTACCTAAATTTGGCTAAATCGTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGATATAACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCa                                                   <  1:302899/150‑1 (MQ=255)
                                                                                            aagTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAg                                                  <  2:1674383/148‑1 (MQ=255)
                                                                                                 tttAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCCCCACGTATGCTTTGCGTCCCCTTACTATCAGGACGc                                             >  1:438505/1‑150 (MQ=255)
                                                                                                   tAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCtt                                           <  1:1088300/150‑1 (MQ=255)
                                                                                                    aaCGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTAACCTTACTATCAGGACGCtta                                          >  1:716727/1‑149 (MQ=255)
                                                                                                    aaCGTGTTACCTAAATGTGGCTAAATCCTTCAAGAAGCCTGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCttt                                          <  1:953480/150‑1 (MQ=255)
                                                                                                            aCCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATg                                  >  2:693754/1‑150 (MQ=255)
                                                                                                            aCCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATACGCTGGCTTCTTGCCTCTCAGGAAATAACTTATGTCCAAATGGCAACTCGCCAGATCCTCCTTCACCACGTATGCTTAGCGTCACCTAACTATCAGGACGCTTTAGCACATg                                  >  1:1775332/1‑150 (MQ=255)
                                                                                                             ccTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGt                                 >  1:517882/1‑150 (MQ=255)
                                                                                                                aaaTTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAATTCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTccc                              <  1:55624/150‑1 (MQ=255)
                                                                                                                aaaTTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTccc                              <  2:284563/150‑1 (MQ=255)
                                                                                                                    ttGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCtt                          <  1:955713/150‑1 (MQ=255)
                                                                                                                       gCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCttttt                       <  1:1017527/150‑1 (MQ=255)
                                                                                                                       gCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCttttt                       <  1:1424420/150‑1 (MQ=255)
                                                                                                                        cTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTg                      <  1:625025/150‑1 (MQ=255)
                                                                                                                        cTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCAAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTg                      >  1:876362/1‑150 (MQ=255)
                                                                                                                         tAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCATGACGCTTTAGCCCATGTCCCGCTTTTTGa                     >  1:1208036/1‑150 (MQ=255)
                                                                                                                              tcTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGGCTCTCAGGAAATAACTTATGTCCAAATGGCAACTCGCCTGATACTCCTTCACCACGTTTGCTTTTCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGt                <  1:1524577/149‑1 (MQ=255)
                                                                                                                              gaggcAAGAAGCCAGCGAATGGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGt                >  2:223646/5‑150 (MQ=255)
                                                                                                                                ggcAAGAAGCCAGCGAATGGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTAGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAg              >  2:1001118/3‑150 (MQ=255)
                                                                                                                                   aagaagCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGTCGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGatt           >  2:1119466/1‑150 (MQ=255)
                                                                                                                                     gaagCCAGCGAATGGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTg         >  1:1414343/1‑150 (MQ=255)
                                                                                                                                     gaagCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGttttt         >  2:1505745/1‑149 (MQ=255)
                                                                                                                                      aagCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCCGGACGCTTTAGCCGATGGCGGGCGTTTTGATTTTTAGTTTTGc        >  2:295086/1‑150 (MQ=255)
                                                                                                                                       agccagccATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGcc       <  1:479579/150‑1 (MQ=255)
                                                                                                                                        gccagccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGccc      <  2:1656508/150‑1 (MQ=255)
                                                                                                                                         ccagccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCt     <  1:805803/150‑1 (MQ=255)
                                                                                                                                          cagccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTg    >  1:557662/1‑150 (MQ=255)
                                                                                                                                          cagccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTg    <  1:1345763/150‑1 (MQ=255)
                                                                                                                                          cagccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTg    >  1:1100874/1‑150 (MQ=255)
                                                                                                                                          cagccaTTCGCTGGCTTCTTGACTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCATACTTCACCAAGTATGCTTTGAGTCACCTTAATATCAGGACGCTTTAGCACATGTCCAGCTTTTTGATTTGTAGTTTTGCCCTg    >  1:1150981/1‑150 (MQ=255)
                                                                                                                                           agccaTTCGCTGGCTTCTTGCCTCTCCGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCCCCACGTATGCGTTGCGTCACCTTACTATCACGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTgg   >  2:520179/1‑150 (MQ=255)
                                                                                                                                            gccaTTCGCTGGCTTCTTGCCTCTAAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGt  <  2:14164/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TCCCAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGT  >  NC_000913/3891360‑3891649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: