Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,947,332 A→T 5.6% A212A (GCT→GCA hdfR ← flhDC operon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,947,3320AT 5.6% 328.1 / 5.4 114A212A (GCT→GCAhdfRflhDC operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (50/56);  new base T (4/3);  total (54/60)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 118 total aligned reads displayed.

TTATCGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGAAATA  >  NC_000913/3947183‑3947481
                                                                                                                                                     |                                                                                                                                                     
ttATCGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTca                                                                                                                                                       <  2:1654416/150‑1 (MQ=255)
     gCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCATCCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGa                                                                                                                                                  >  1:79327/1‑150 (MQ=255)
         ttttGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTg                                                                                                                                              <  1:1817313/150‑1 (MQ=255)
            tGCAGCCATATGGCTTAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCTTTAATCATCGCTATCTGTTGCTGTGCCAGTTCAGCAGAACTGGtt                                                                                                                                           <  1:1110102/150‑1 (MQ=255)
             gCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTg                                                                                                                                          <  1:18977/150‑1 (MQ=255)
              cAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCTGCTGAACTggcaca                                                                                                                                         <  1:1572039/150‑5 (MQ=255)
              cAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGATCTGGTTGt                                                                                                                                         >  1:131134/1‑150 (MQ=255)
              cAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGt                                                                                                                                         >  1:1013458/1‑150 (MQ=255)
               aGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCTGCTGAACTggcacag                                                                                                                                        <  2:1694419/150‑6 (MQ=255)
               aGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTc                                                                                                                                        <  2:843218/150‑1 (MQ=255)
                gCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGGTCAGCAGAACTGGTTGTCa                                                                                                                                       <  1:323858/150‑1 (MQ=255)
                 ccATATGTCATAATCCGGCCGTGTAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTTGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGGGCCAGTTCCGCAGAACTGGTTGTCAg                                                                                                                                      <  2:1638755/150‑1 (MQ=255)
                 ccATATGGCATAAAGCGGCCGTGAACGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAg                                                                                                                                      <  1:913203/150‑1 (MQ=255)
                  cATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGa                                                                                                                                     >  2:1553108/1‑150 (MQ=255)
                        gCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCTGAATGGGc                                                                                                                               >  1:382661/1‑150 (MQ=255)
                        gCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGc                                                                                                                               <  1:1789174/150‑1 (MQ=255)
                        gCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGCGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGc                                                                                                                               <  1:856523/150‑1 (MQ=255)
                           tAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACt                                                                                                                            >  2:1725126/1‑150 (MQ=255)
                           tAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTAAGCAGAACTGGTTGGCAGAATGGGCACt                                                                                                                            >  2:43616/1‑150 (MQ=255)
                               gCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGt                                                                                                                        <  2:1193520/150‑1 (MQ=255)
                                 ggCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCa                                                                                                                      <  1:701069/150‑1 (MQ=255)
                                      tGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCTGGTGCAACCATTAAGCTTCGCAATCTGGTGGTGTGCCAGTTCAGCAGAACTGGTTGTCTGAATGGGCACTTCGTCAGCAcc                                                                                                                 >  2:306063/1‑150 (MQ=255)
                                      agAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTAACGCGCCAAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGAACTTCGTCAGCaac                                                                                                                 >  2:1418507/2‑148 (MQ=255)
                                       gAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCg                                                                                                                <  2:1102632/150‑1 (MQ=255)
                                       gAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCg                                                                                                                <  2:1712982/150‑1 (MQ=255)
                                         aaGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGAt                                                                                                              <  1:1656693/150‑1 (MQ=255)
                                              ttgtGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAAc                                                                                                         <  2:1159319/150‑1 (MQ=255)
                                              ttgtGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAAc                                                                                                         <  1:860380/150‑1 (MQ=255)
                                               tgtgCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAAcc                                                                                                        <  1:1506476/150‑1 (MQ=255)
                                                  gCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGc                                                                                                     >  1:1069744/1‑150 (MQ=255)
                                                   cTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCACCCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGcc                                                                                                    >  2:522247/1‑150 (MQ=255)
                                                   cTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGcc                                                                                                    <  1:1070531/150‑1 (MQ=255)
                                                     aTCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTc                                                                                                  <  1:1494125/150‑1 (MQ=255)
                                                       cGACAACGGTATGCATGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAAGATGTTGCTGTGCCAGTTCAGCAGAAGTGGTTGTCAGAATGGGCACTTCGTCAGACCAGAGCAAACCTGCcacag                                                                                                >  2:319996/1‑149 (MQ=255)
                                                        gACAACGGTAGGCAGGCCGCCTTTTTTACTCTCCCAGCTGACGGGTAGCCAGGGGCAACCATTAAGCATCGCAATCTGTTGCTGGGCCTGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATg                                                                                               <  2:1654897/150‑1 (MQ=255)
                                                          cAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGCCGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGTTTGTCAGAATGGGCACATCGTCAGCCCCGATCAAACCTGCCTCATgat                                                                                             >  2:1318129/1‑148 (MQ=255)
                                                            aCGGTCTGCAGGCCGCCTTTATTACGCGCCCAGCTGCCGGGTAGCCAGGTGCAACCATTACGCATCGCAATCTGTGTCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGt                                                                                           <  1:137883/150‑1 (MQ=255)
                                                            aCGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCCCTTCGTCAGCACCGATCTAACCTGCCTCATGCTgg                                                                                           >  1:1527939/1‑149 (MQ=255)
                                                                tATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGaa                                                                                       <  1:832167/150‑1 (MQ=255)
                                                                 aTGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGaaa                                                                                      <  1:1271269/150‑1 (MQ=255)
                                                                     aGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCGGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCt                                                                                  <  1:694519/150‑1 (MQ=255)
                                                                     aGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCt                                                                                  <  2:1019788/150‑1 (MQ=255)
                                                                        ccgccTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGc                                                                               >  1:1803815/1‑150 (MQ=255)
                                                                        ccgccTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGc                                                                               <  2:445977/150‑1 (MQ=255)
                                                                            cTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGccccc                                                                           >  2:1518636/1‑150 (MQ=255)
                                                                            cTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGGTGTCAGAATGGGCACTTCGTCAGCAGCGATCACACCTGCCTCATGCTGGTGAAAATCTGGccccc                                                                           >  1:299850/1‑150 (MQ=255)
                                                                              tttttACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCAc                                                                         <  2:1779668/150‑1 (MQ=255)
                                                                               ttttACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACt                                                                        >  1:1585749/1‑150 (MQ=255)
                                                                                  tACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCaa                                                                     >  2:734985/1‑150 (MQ=255)
                                                                                   aCGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAg                                                                    <  1:204318/150‑1 (MQ=255)
                                                                                       gcCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGc                                                                >  2:11654/1‑150 (MQ=255)
                                                                                       gcCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGc                                                                >  1:1418722/1‑150 (MQ=255)
                                                                                       gcCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGc                                                                >  2:18273/1‑150 (MQ=255)
                                                                                        cccAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCa                                                               <  2:1160763/150‑1 (MQ=255)
                                                                                        cccAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCa                                                               <  1:794587/150‑1 (MQ=255)
                                                                                           aGCTGACGGGTAGCCAGGTGCAACCATTAAGCATAGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCACCACACAAGTCCCAGaa                                                            >  2:133086/1‑149 (MQ=255)
                                                                                              tGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAAt                                                         <  2:1402114/150‑1 (MQ=255)
                                                                                              tGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAAt                                                         <  1:1281435/150‑1 (MQ=255)
                                                                                                    gTAGCCAGGTGCAACCATTAAGCATCTCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGTGCACTTCGTCAGCACCGATCAAAACTTCCTCTTGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGAt                                                   <  1:298510/150‑1 (MQ=255)
                                                                                                       gCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGTGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGAtctc                                                <  2:814121/150‑1 (MQ=255)
                                                                                                       gCCAGGGGCAACCATTAATCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGGTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGAtctc                                                <  2:821201/150‑1 (MQ=255)
                                                                                                        ccAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTcc                                               >  2:609521/1‑150 (MQ=255)
                                                                                                         cAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTccc                                              >  1:25005/1‑150 (MQ=255)
                                                                                                           ggTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCtt                                            <  2:1013458/150‑1 (MQ=255)
                                                                                                           ggTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCtt                                            >  2:763785/1‑150 (MQ=255)
                                                                                                            gTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCttt                                           <  2:236310/150‑1 (MQ=255)
                                                                                                            gTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCCATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGAAGATAATAAAGATCTCCCttt                                           >  1:692807/1‑150 (MQ=255)
                                                                                                              gCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAAGTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGCCACCCCCTCAAGTCGCAGCTAAGTAAGATCTCCCTTGAg                                         >  2:337975/1‑150 (MQ=255)
                                                                                                                  ccATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGCTCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAACTCGCAGATAATTAACATCTCCCTATAGtttt                                     >  1:893065/1‑150 (MQ=255)
                                                                                                                  ccATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGtttt                                     >  1:1672026/1‑150 (MQ=255)
                                                                                                                   cATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTg                                    <  2:1866534/150‑1 (MQ=255)
                                                                                                                     ttAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGaa                                  <  2:25005/150‑1 (MQ=255)
                                                                                                                       aaGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAgg                                <  1:1725126/150‑1 (MQ=255)
                                                                                                                        aGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAggg                               <  1:726160/150‑1 (MQ=255)
                                                                                                                        aGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAggg                               <  1:1367771/150‑1 (MQ=255)
                                                                                                                        aGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCGCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTATAGTTTTGAAggg                               >  2:897987/1‑150 (MQ=255)
                                                                                                                          cATCGCATTCTGTTGCTGTGCCAGTTCAGCAGAACTGGCTGTCAGAATGGGCACTTCGTGAGCACCGATCAAACCTGCCTCATTCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGAAAATTAAGATCTCCCTTTAGTTTTGAAGGGGc                             <  2:1372397/150‑1 (MQ=255)
                                                                                                                          cATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGTCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGc                             <  2:570262/150‑1 (MQ=255)
                                                                                                                             cGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACt                          >  2:450472/1‑150 (MQ=255)
                                                                                                                             cGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCAAGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACt                          >  2:60822/1‑150 (MQ=255)
                                                                                                                              gCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAACCCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTg                         >  1:967183/1‑150 (MQ=255)
                                                                                                                                aaTCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGt                       >  2:397467/1‑150 (MQ=255)
                                                                                                                                  tCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGtat                     <  2:28833/150‑1 (MQ=255)
                                                                                                                                   cTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGGTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGtata                    <  2:411563/150‑1 (MQ=255)
                                                                                                                                      ttGCTGTGCCAGTTCAGCAGAACTGGGTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCCTGCTGTTGAAAATCTGGCCCCCACTCAAGGCGCAGATATTTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAg                 <  2:1720443/150‑1 (MQ=255)
                                                                                                                                       tGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAgc                >  2:1309263/1‑150 (MQ=255)
                                                                                                                                       ctgacaaccAGTTCTGCTGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAgc                >  2:1547155/8‑150 (MQ=255)
                                                                                                                                         cTGTGCCAGTTCTGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAgcgc              <  2:1607554/150‑1 (MQ=255)
                                                                                                                                         cTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAgcgc              <  1:292960/150‑1 (MQ=255)
                                                                                                                                         cTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGAAGATAATTAAGATCTCCCTTTAGTATTGAAGGGGCACAGGTATAAAgcgc              >  1:21837/1‑150 (MQ=255)
                                                                                                                                           gtgCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTACGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAGTCTGGCCCCCACTCAAGGCGCAGATAATTAAGATCTCCCTTTAGTGGTGAAGGGGCACTGGTATAAAGCGATa            >  2:1158315/1‑150 (MQ=255)
                                                                                                                                           caaccAGTTCTGCTGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTa            >  1:1613213/4‑150 (MQ=255)
                                                                                                                                           caaccAGTTCTGCTGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTa            >  1:415613/4‑150 (MQ=255)
                                                                                                                                           caaccAGTTCTGCTGAACAGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCAGCCTCATGCTGTTGAAAATATGGCCCCCACTCAAGTCGCAGATTAGTAAGATCTCCCGTTAGTTTTGAAGGGGCACGGGTATAAAGCGGTa            >  2:227429/4‑150 (MQ=255)
                                                                                                                                              ccAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAg         <  1:19117/150‑1 (MQ=255)
                                                                                                                                              ccAGGTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGGCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAg         <  2:79327/150‑1 (MQ=255)
                                                                                                                                                 gTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGa      >  1:524988/1‑150 (MQ=255)
                                                                                                                                                    cagcagAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGAAat   >  1:426901/1‑150 (MQ=255)
                                                                                                                                                     agcagAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGAAata  >  1:1535323/1‑150 (MQ=255)
                                                                                                                                                     agcagAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGAAata  >  2:1794299/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTATCGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGAAATA  >  NC_000913/3947183‑3947481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: