Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,000,245 C→T 9.9% intergenic (+100/+47) uvrD → / ← yigE DNA‑dependent ATPase I and helicase II/DUF2233 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,000,2450CT 9.9% 266.7 / 13.9 91intergenic (+100/+47)uvrD/yigEDNA‑dependent ATPase I and helicase II/DUF2233 family protein
Reads supporting (aligned to +/- strand):  ref base C (41/41);  new base T (6/3);  total (47/44)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01

Only 100 of 102 total aligned reads displayed.

AGGGTATTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGCCATCAAGATAA  >  NC_000913/4000096‑4000394
                                                                                                                                                     |                                                                                                                                                      
aGGGTATTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTAcgccgc                                                                                                                                                        <  1:911364/150‑1 (MQ=255)
  ggTATTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCAt                                                                                                                                                      <  1:1325272/150‑1 (MQ=255)
  ggTATTAAATGGCTGGTGGCGGAATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCAt                                                                                                                                                      <  1:1797317/150‑1 (MQ=255)
    tATTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATGCGGCGTAAACGCCCTATcc                                                                                                                                                    <  1:1620933/150‑1 (MQ=255)
    tATTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATcc                                                                                                                                                    <  1:390126/150‑1 (MQ=255)
    tATTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATcc                                                                                                                                                    <  1:1810609/150‑1 (MQ=255)
     aTTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCCGATAGGGCGTTTACGCCGCATCCg                                                                                                                                                   >  1:54729/1‑150 (MQ=255)
      ttAAATGGCTGGGGCCGGCATACGCCCGGCTGGAGTCGGGGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATGCGGCGTAAACGCCCTATCCgg                                                                                                                                                  <  1:185504/150‑1 (MQ=255)
        aaaTGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCCCCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCa                                                                                                                                                >  2:668367/1‑150 (MQ=255)
        aaaTGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCa                                                                                                                                                <  1:72250/150‑1 (MQ=255)
        aaaTGGCTGGTGGCGGCATACGCCCGGCTGGAGGCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCa                                                                                                                                                <  2:1798426/150‑1 (MQ=255)
           tGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATGCGGCGTAAACGCCCTATCCGGcctac                                                                                                                                             <  2:150374/150‑5 (MQ=255)
             gCTGGTGGCGGCATACGCCCGGCTCGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTCCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGAATCTgc                                                                                                                                           >  2:777698/1‑150 (MQ=255)
              cTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATGCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTgcg                                                                                                                                          >  2:942810/1‑150 (MQ=255)
                 gtggCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCa                                                                                                                                       <  1:495181/150‑1 (MQ=255)
                      ggcATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTc                                                                                                                                  <  1:1547613/150‑1 (MQ=255)
                      ggcATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTc                                                                                                                                  <  2:1047794/150‑1 (MQ=255)
                        cATACGCCCGTCTGGAGTCGTTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAg                                                                                                                                <  1:1433559/150‑1 (MQ=255)
                         aTACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCAGTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGt                                                                                                                               >  2:218905/1‑150 (MQ=255)
                            cGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATc                                                                                                                            >  2:1088381/1‑150 (MQ=255)
                              cccGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTg                                                                                                                          <  1:1660240/150‑1 (MQ=255)
                              cccGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTg                                                                                                                          <  2:1495710/150‑1 (MQ=255)
                                   cTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGAcaca                                                                                                                     >  1:36320/1‑150 (MQ=255)
                                           ggTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATATGACACAAAACTATc                                                                                                             >  1:1343357/1‑150 (MQ=255)
                                            gtgtAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCg                                                                                                            <  2:1235000/150‑1 (MQ=255)
                                              gtAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGtt                                                                                                          <  2:870772/150‑1 (MQ=255)
                                              gtAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGtt                                                                                                          <  2:862924/150‑1 (MQ=255)
                                                aaCGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGtttt                                                                                                        <  2:1092282/150‑1 (MQ=255)
                                                 aCGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTa                                                                                                       <  2:1234796/150‑1 (MQ=255)
                                                 aCGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTa                                                                                                       <  2:81586/150‑1 (MQ=255)
                                                  cGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTaa                                                                                                      >  2:231734/1‑150 (MQ=255)
                                                   gTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAAc                                                                                                     <  2:333879/150‑1 (MQ=255)
                                                   gTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAAc                                                                                                     >  2:1757953/1‑150 (MQ=255)
                                                   gTTGCCAGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAAc                                                                                                     <  2:1209139/150‑1 (MQ=255)
                                                     tGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCt                                                                                                   >  1:46898/1‑150 (MQ=255)
                                                         ggATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGTCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTcc                                                                                               <  2:1399786/150‑1 (MQ=255)
                                                         ggATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTcc                                                                                               <  2:1490865/150‑1 (MQ=255)
                                                              cGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCg                                                                                          <  2:926136/150‑1 (MQ=255)
                                                              cGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCg                                                                                          <  2:1086619/150‑1 (MQ=255)
                                                               ggTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGa                                                                                         >  2:1579951/1‑150 (MQ=255)
                                                                        cACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCa                                                                               >  1:639273/1‑150 (MQ=255)
                                                                         aCCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGTAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGTCGAGCGGaaaggtt                                                                              <  1:1380672/150‑5 (MQ=39)
                                                                          ccTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATg                                                                             >  2:1695851/1‑150 (MQ=255)
                                                                          ccTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATg                                                                             >  2:1485875/1‑150 (MQ=255)
                                                                          ccTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGAGTTTACGCCGCATCCGCCATCTGCGGCATCTTCAGTATCTGGCACAAAACTATCGATTTAACCTTTCCGATCGACGGAAATCATg                                                                             >  2:1775609/1‑150 (MQ=255)
                                                                                   gCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACaaaa                                                                    >  1:1830221/1‑150 (MQ=255)
                                                                                    ccTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAg                                                                   >  1:452164/1‑150 (MQ=255)
                                                                                    ccTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAg                                                                   >  1:1367937/1‑150 (MQ=255)
                                                                                    ccTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAg                                                                   >  1:1536249/1‑150 (MQ=255)
                                                                                      tAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATAATGGTGACAAAAGGa                                                                 >  1:1813994/1‑150 (MQ=255)
                                                                                      tAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAAATATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAggt                                                                 >  1:180992/1‑149 (MQ=255)
                                                                                       aaaaaaTCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGAt                                                                <  2:143442/150‑1 (MQ=255)
                                                                                                ttCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGcc                                                       >  1:1637666/1‑150 (MQ=255)
                                                                                                 tCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTTACAAAAGGATAGCGTTGccc                                                      >  2:73074/1‑149 (MQ=255)
                                                                                                     aTTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACggg                                                  <  1:616160/150‑1 (MQ=255)
                                                                                                         aaTAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAACACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCCCGGGATTg                                              >  2:847535/1‑150 (MQ=255)
                                                                                                         aaTAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTg                                              >  1:518435/1‑150 (MQ=255)
                                                                                                         aaTAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTg                                              >  2:1098304/1‑150 (MQ=255)
                                                                                                          aTAAATTGCAACGTCATGTAGGACGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGc                                             <  1:208434/150‑1 (MQ=255)
                                                                                                            aaaTTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGccc                                           >  2:420289/1‑150 (MQ=255)
                                                                                                            aaaTTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGacc                                           >  1:551854/1‑150 (MQ=255)
                                                                                                             aaTTTCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGcccc                                          <  2:778136/150‑1 (MQ=255)
                                                                                                             aaTTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGcccc                                          <  2:778133/150‑1 (MQ=255)
                                                                                                                  cAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCtt                                     >  2:867472/1‑150 (MQ=255)
                                                                                                                    aCGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTc                                   <  1:1251158/150‑1 (MQ=255)
                                                                                                                    aCGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTc                                   <  2:1536249/150‑1 (MQ=255)
                                                                                                                     cGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCa                                  <  2:1418048/150‑1 (MQ=255)
                                                                                                                     cGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCAGCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCa                                  <  1:305744/150‑1 (MQ=255)
                                                                                                                      gTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCat                                 >  2:848769/1‑150 (MQ=255)
                                                                                                                       tCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCata                                <  2:645109/150‑1 (MQ=255)
                                                                                                                        cATGTAGGCCGGATAGGGCGTTTACGCCGCATCAGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAGTCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCatat                               >  1:548624/1‑150 (MQ=255)
                                                                                                                         gcagatgCCGGATGCGGCGTAAACGCCCTATCCGGCATCTGCGCAATCTTCAGTATCTGACACAAAACTATCGTTTTATCCTTTCCGCTCGACGGAATTCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCAtata                              >  1:1098981/7‑150 (MQ=38)
                                                                                                                          cagatgCCGGATGCGGCGTAAACGCCCTATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATAc                             >  1:116344/6‑150 (MQ=255)
                                                                                                                            tAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACat                           >  1:455874/1‑150 (MQ=255)
                                                                                                                            gatgCCGGATGCGGCGTAAACGCCCTATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACat                           >  1:1628669/4‑150 (MQ=255)
                                                                                                                             cggCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCATCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACata                          <  2:1577908/149‑1 (MQ=255)
                                                                                                                             aGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACata                          >  2:669876/1‑150 (MQ=255)
                                                                                                                              tgCCGGATGCGGCGTAAACGCCCTATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGCCAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCGTTCATATACatat                         >  2:1737029/2‑150 (MQ=255)
                                                                                                                              tgCCGGATGCGGCGTAAACGCCCTATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTATCATATACattt                         >  2:1324546/2‑148 (MQ=255)
                                                                                                                              tgCCGGATGCGGCGTAAACGCCCTATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACGATCGTGTTACCCTTTCCGCTCGACGGAAATCATGGGGACAACAGGATAGCGTTGCCACGGGATTGCCCCGCCTTGCATATACatat                         >  2:1318213/2‑150 (MQ=255)
                                                                                                                               gCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATAtg                        <  1:1741157/150‑1 (MQ=255)
                                                                                                                               gCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATAtg                        >  1:1488200/1‑150 (MQ=255)
                                                                                                                                ccGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGCCGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATAtgt                       >  1:1588615/1‑150 (MQ=255)
                                                                                                                                ccGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATAtgt                       >  1:1749430/1‑150 (MQ=255)
                                                                                                                                     tAGGGCGGTTACGCCGCATGCGGCATCTGCGCCATCTTCAGTATGTGACACAAAACTATCGTTTTAACCTTTGCGCTCGACGGAAATCATTGTGACAAAAGGATAGGGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGaatt                  <  2:1478030/150‑3 (MQ=255)
                                                                                                                                        ggCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGt               >  1:1606757/1‑150 (MQ=255)
                                                                                                                                        ggCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGt               >  1:387532/1‑150 (MQ=255)
                                                                                                                                          cGTTTACGCCGCATCCGGCAGCTGCGCCATCTTCAGTATCTGACACAATACTATCGTTTTAACCTTTCCGATCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGc             <  1:319502/150‑1 (MQ=255)
                                                                                                                                            tttACGCCGCATCCGGCATCTGCGACATCTTCAGTATATGACACAAAACTATCGTTTTAACATTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGTATTGCCCCGCATTTCAAATACATATGTGAAATCGTGCCa           <  1:1225649/150‑1 (MQ=255)
                                                                                                                                               aCGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGCCATCa        <  1:1088381/150‑1 (MQ=255)
                                                                                                                                                cgccgcATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGCCATCaa       >  2:1460456/1‑150 (MQ=255)
                                                                                                                                                cgccgcATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGCCATCaa       <  2:36320/150‑1 (MQ=255)
                                                                                                                                                cgccgcATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGCCATCaa       >  1:880966/1‑150 (MQ=255)
                                                                                                                                                cgccgcATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGCCATCaa       <  1:867472/150‑1 (MQ=255)
                                                                                                                                                cgccgcATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTAGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGGCATCaa       >  1:182111/1‑150 (MQ=255)
                                                                                                                                                 gccgcATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGCCATCAAg      >  1:473957/1‑150 (MQ=255)
                                                                                                                                                 gccgcAGACGGCATCTGCGCAATCTTCAGTATCTGACTCAAAACTATCGTGTAAACCTTTCCGCTCGACGGAATTCATGGTGTCAAAAGGATAGCGTGGCCACGGGAGTGCCCCGCCTTTCATATACATATGTGAAATCGTGCCATCAAg      <  2:279247/150‑1 (MQ=255)
                                                                                                                                                  ccgcATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTAGCGTTTTAACCTTTCCGCTCGCCGGAAATCATGGTGACAAAAGGATAGCCTTGCCACGGGCTTGCCCCGCCTGTCTGATCCATATGTGATATCGTGCCATAAAGa     >  2:1126428/1‑150 (MQ=255)
                                                                                                                                                   cgcATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGCCATCAAGAt    >  2:855843/1‑150 (MQ=255)
                                                                                                                                                     cATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTCCCATCAAGATaa  >  2:1779764/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AGGGTATTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGCCATCAAGATAA  >  NC_000913/4000096‑4000394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: