BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,009,779	T→G	5.2%	L204V (TTG→GTG)	pldB →	lysophospholipase L2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,009,779	0	T	G	5.2%	392.3 / 7.2	138	L204V (TTG→GTG)	pldB	lysophospholipase L2
Reads supporting (aligned to +/- strand): ref base T (65/65); new base G (5/3); total (70/68)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 143 total aligned reads displayed.

GTGTATGTGACGCCATTGCGCTAACTGCGCCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCC  >  NC_000913/4009630‑4009928

gtgtATGTGACGCCATTGCGCTAACTGCGCCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGAGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGt                                                                                                                                                       >  1:297528/1‑150 (MQ=255)

 tgtATGTGACGCCATTGCGCTAACTGCGCCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGtt                                                                                                                                                      >  1:1863740/1‑150 (MQ=255)

 tgtATGTGACGCCATTGCGCTAACTGCGCCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATACACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGtt                                                                                                                                                      >  2:509346/1‑150 (MQ=255)

  gtATGTGACGCCATTGCGCTAACTGCGCCAATGTTTGGGTTCGTGATGCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTg                                                                                                                                                     <  2:266455/150‑1 (MQ=255)

  gtATGTGACGCCATTGCGCTAACTGCGCCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTg                                                                                                                                                     <  2:1632157/150‑1 (MQ=255)

     tgtgACGCCATTGCGCTAACTGCGCCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCg                                                                                                                                                  >  1:671951/1‑150 (MQ=255)

      gtgACGCCATTGCGCTAACTGCGCCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGt                                                                                                                                                 >  2:1563303/1‑150 (MQ=255)

       tgACGCCATTGCGCTAACTGCGCCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGtt                                                                                                                                                <  2:1782470/150‑1 (MQ=255)

         aCGCCATTGCGCTAACTGCGCCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTg                                                                                                                                              <  1:1449716/150‑1 (MQ=255)

          cGCCATTGCGCTAACTGCGCCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCTCCGGGCGCTGGCGCGCGTTGCAGTATGc                                                                                                                                             >  1:158739/1‑150 (MQ=255)

          cGCCATTGCGCTAACTGCGCCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGc                                                                                                                                             <  1:626338/150‑1 (MQ=255)

             cATTGCGCTAACTGCGCCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTAt                                                                                                                                          <  1:1302979/150‑1 (MQ=255)

              aTTGCGCTAACTGCGCCAATGGTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGAGCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATc                                                                                                                                         <  2:937705/150‑1 (MQ=255)

                tGCGCTAACTGCGCCAATGTGTGGGAGCGTGATTCGTATCCCGTCATATATGGCACGGCAGATCCTCATCTGGGCCGAAGCGCAGCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCaa                                                                                                                                       <  2:85389/150‑1 (MQ=255)

                    gtaccTGCGCGAAGGTTTGGGCTCGTGATTCGTATGCCGTCATTTATGGGCCGGCAGATCCTCACCTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTa                                                                                                                                   <  1:167047/146‑1 (MQ=255)

                      aaCTGCGCCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCACCGTACt                                                                                                                                 >  1:184453/1‑150 (MQ=255)

                       aCTGCGCCAATGTTTGGGATCGTGATTCGTGTGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTg                                                                                                                                >  2:1136201/1‑150 (MQ=255)

                        cTGCGCCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGa                                                                                                                               >  2:1415403/1‑150 (MQ=255)

                         tGCGCCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGAc                                                                                                                              <  2:1210753/150‑1 (MQ=255)

                          gcgcCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGAcc                                                                                                                             <  2:317519/150‑1 (MQ=255)

                          gcgcCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGAcc                                                                                                                             >  2:933859/1‑150 (MQ=255)

                           cgcCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGcccc                                                                                                                            >  1:215243/1‑150 (MQ=255)

                           atcctaTGTTTGTGATCGTGATTCGTATGCCGTCATTTATTGCAATGCAGATCCTCAACTGGGCAGAAGCGGATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGAccc                                                                                                                            <  2:97814/145‑1 (MQ=255)

                            gcCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGAcccc                                                                                                                           >  2:1222043/1‑149 (MQ=255)

                              cAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCcaca                                                                                                                         >  1:1023322/1‑150 (MQ=255)

                              cAATGTTTGGGATCGTGATTCGTAGGCCGTCATTTATGGCCCGGCAGATCCTCACCTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCcaca                                                                                                                         <  2:359423/150‑1 (MQ=255)

                                aTGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCAcagc                                                                                                                       <  1:763109/150‑1 (MQ=255)

                                  gTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCAcagcag                                                                                                                     <  1:1655479/150‑1 (MQ=255)

                                    ttGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCCCAGCAGAc                                                                                                                   >  2:1088544/1‑150 (MQ=255)

                                     tGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACa                                                                                                                  >  2:1066471/1‑150 (MQ=255)

                                       ggATCGTGATTCGTATGCCGTCATTTATGGCACGGCTGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGc                                                                                                                <  1:1712866/150‑1 (MQ=255)

                                       ggATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGc                                                                                                                >  2:460199/1‑150 (MQ=255)

                                        gATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCg                                                                                                               >  1:855/1‑150 (MQ=255)

                                         ctCGTGATTCGTACGCCGTCATTTATGGCACGGCAGAGCCTCACCTGGGCCGAAGCGCATCCCCGTTTCCGGGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGa                                                                                                              <  2:297528/149‑1 (MQ=255)

                                         aTCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGa                                                                                                              <  1:1227433/150‑1 (MQ=255)

                                         aTCGTGATGCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGa                                                                                                              <  2:231121/150‑1 (MQ=255)

                                           cGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCACCGTACTGACCCACAGCAGACAGCGata                                                                                                            >  2:865584/1‑150 (MQ=255)

                                            gTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGatat                                                                                                           >  1:1752769/1‑150 (MQ=255)

                                                 tagTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACg                                                                                                      <  1:1810035/148‑1 (MQ=255)

                                                 tCGTATGGCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCTCGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACg                                                                                                      <  1:1584238/150‑1 (MQ=255)

                                                 tCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACg                                                                                                      <  1:460986/150‑1 (MQ=255)

                                                     aTGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAAc                                                                                                  >  1:1386639/1‑150 (MQ=255)

                                                     aTGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAAc                                                                                                  >  2:1296820/1‑150 (MQ=255)

                                                     aTGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAAc                                                                                                  <  1:619183/150‑1 (MQ=255)

                                                     aTGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAAc                                                                                                  <  1:809487/150‑1 (MQ=255)

                                                        ccGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTa                                                                                               >  1:980766/1‑150 (MQ=255)

                                                            cATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGTACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCt                                                                                           >  1:706394/1‑150 (MQ=255)

                                                             aTTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCtt                                                                                          >  2:1287210/1‑150 (MQ=255)

                                                             aTTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCtt                                                                                          >  1:1123669/1‑150 (MQ=255)

                                                                tATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTTCTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCtt                                                                                       >  2:1645376/1‑149 (MQ=255)

                                                                  tGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACTCTTCTATg                                                                                     >  2:949529/1‑150 (MQ=255)

                                                                  tGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACGGACCCACAGCAGACAGCGCTATCGACGTAACTTACGCTTCTATg                                                                                     >  2:1577599/1‑150 (MQ=255)

                                                                     cACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGCCGTAACTTACGCTTCTATGCtg                                                                                  >  2:397291/1‑150 (MQ=255)

                                                                     cACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCtg                                                                                  <  2:480733/150‑1 (MQ=255)

                                                                        ggCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCtgatg                                                                               <  1:470159/150‑1 (MQ=255)

                                                                         gCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCtgatga                                                                              >  1:1841087/1‑150 (MQ=255)

                                                                               ccTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAAc                                                                        <  1:981467/150‑1 (MQ=255)

                                                                               ccTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAAc                                                                        <  2:421013/150‑1 (MQ=255)

                                                                                cTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACg                                                                       <  1:1462168/150‑1 (MQ=255)

                                                                                    aCTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTc                                                                   >  2:57769/1‑150 (MQ=255)

                                                                                     cTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTcg                                                                  <  1:7753/150‑1 (MQ=255)

                                                                                     cTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTcg                                                                  >  1:1124850/1‑150 (MQ=255)

                                                                                       gggCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTcgcg                                                                <  1:1611076/150‑1 (MQ=255)

                                                                                          ccGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCg                                                             >  1:1736034/1‑150 (MQ=255)

                                                                                          ccGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCg                                                             >  1:404084/1‑150 (MQ=255)

                                                                                               gcgcATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTggg                                                        <  2:256344/150‑1 (MQ=255)

                                                                                                 gcATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGcc                                                      <  1:1057720/150‑1 (MQ=255)

                                                                                                 gcATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGcc                                                      <  2:695204/150‑1 (MQ=255)

                                                                                                  cATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGACGGTGGGCCg                                                     >  1:675053/1‑150 (MQ=255)

                                                                                                      cACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCt                                                 >  1:447908/1‑150 (MQ=255)

                                                                                                         gTTTCCGTTCTGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACCGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTAcc                                              <  1:195761/150‑1 (MQ=255)

                                                                                                             ccGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGAAACTTACGCGTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTg                                          <  2:1813478/150‑1 (MQ=255)

                                                                                                               gTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTggg                                        <  2:1033537/150‑1 (MQ=255)

                                                                                                                 gATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCGGCTATGCTGTTGACGCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTa                                      <  2:661725/150‑1 (MQ=255)

                                                                                                                   tGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTAcg                                    <  1:561517/150‑1 (MQ=255)

                                                                                                                     gCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTAcgcg                                  >  1:545097/1‑150 (MQ=255)

                                                                                                                     gCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTAcgcg                                  <  1:1553449/150‑1 (MQ=255)

                                                                                                                      cTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCCACGATTCGCGTCGGTGGGCCGCCCTACCATTGGGTACGCGa                                 >  1:369190/1‑150 (MQ=255)

                                                                                                                      cTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGa                                 >  2:1662706/1‑150 (MQ=255)

                                                                                                                      cTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGa                                 >  1:1861224/1‑150 (MQ=255)

                                                                                                                      cTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTAAGCTGATGACCAAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGa                                 >  2:202129/1‑150 (MQ=255)

                                                                                                                         tGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTACCAACGTACTGACCCACAGCAGACAGCGATATCGACGAAACGTACGCTTCTATGCTGATGACCCACCGATTCGCGGCGGTGGGCCGACCTACCATTGGGTACGCGAAAg                              <  2:1335195/150‑1 (MQ=255)

                                                                                                                         tGCAATAGGCACAGGGCGCTGGCGCGCGGTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACCATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAg                              <  2:1328743/150‑1 (MQ=255)

                                                                                                                          gcaaacggCAACGCGCGCCAGCGCCCGGTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGt                             >  2:760577/7‑150 (MQ=255)

                                                                                                                           caaacggCAACGCGCGCCAGCGCCCGGTGCCGTTTGCTATCAACGTACTGCCCCCCAGCAGACAGCGAGATCGCCGTAACTTACGCTTCTATGCTGCTGACCCCACGCTTCGCGTCGGTGGGCCGCCCTACCATTGGGTCCGCGAAAGtg                            >  1:69546/6‑149 (MQ=255)

                                                                                                                           cAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTa                            >  1:1809670/1‑150 (MQ=255)

                                                                                                                             aacggCAACGCGCGCCAGCGCCCGGTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGCCGTAACTTACGCTTCTATGCTGATGACCAAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCTAAAGTAtt                          >  2:1075544/4‑150 (MQ=255)

                                                                                                                              tAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGCCCTACCATTGGGTACGCGAAAGTATTc                         >  1:1384834/1‑150 (MQ=255)

                                                                                                                                ggCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTctg                       >  1:250284/1‑150 (MQ=255)

                                                                                                                                 gCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTctgg                      <  1:1287210/150‑1 (MQ=255)

                                                                                                                                  cACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGAACTGACCCACAGCAGACAGCGATAGCGACGTAACTTTCGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTctggc                     <  1:159761/150‑1 (MQ=255)

                                                                                                                                  cACCGGGCGCTGGCGCGCGGTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGGGGGCCGACCTACCATTGGGTACGCGAAAGTATTctggc                     <  2:506114/150‑1 (MQ=255)

                                                                                                                                   aCCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTctggct                    <  2:1724264/150‑1 (MQ=255)

                                                                                                                                       ggCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCg                <  1:1066471/150‑1 (MQ=255)

                                                                                                                                       ggCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGAGATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGCCCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCg                >  1:420121/1‑150 (MQ=255)

                                                                                                                                        gcgcTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGCCCTAGCATGGGGTACGCGAAAGTATTCTGGCGGGCGa               >  2:371253/1‑150 (MQ=255)

                                                                                                                                          gcTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAAc             <  1:940753/150‑1 (MQ=255)

                                                                                                                                               cgcgcgTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGgtg        <  1:1296820/150‑1 (MQ=255)

                                                                                                                                                gcgcgTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGgtgt       >  1:638332/1‑150 (MQ=255)

                                                                                                                                                     ttGCCGTTTGCTATCAACGTACTGACCCACAGCTGACTGCGATATCGACGTAACTTACGCTTGTTTGCTGATGACCCTACGATTCGCGTCGGTGGGCCGACCTACCATTGGTTACGCGATAGTATTCTGGCTGGCGAACAGGTGTTAGcc  <  1:1156302/150‑1 (MQ=255)

GTGTATGTGACGCCATTGCGCTAACTGCGCCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCATCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACAGCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGGGTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCC  >  NC_000913/4009630‑4009928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`