Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,094,489 G→A 7.2% intergenic (‑217/+234) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,094,4890GA 7.2% 261.6 / 10.4 111intergenic (‑217/+234)rhaD/rhaArhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
Reads supporting (aligned to +/- strand):  ref base G (53/49);  new base A (3/5);  total (56/55)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-01

Only 100 of 112 total aligned reads displayed.

ATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGA  >  NC_000913/4094343‑4094638
                                                                                                                                                  |                                                                                                                                                     
aTCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTg                                                                                                                                                    <  2:496685/150‑1 (MQ=32)
   cGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTAATGCCGACCTGTAGGCCTGATAAGGCACTAGTGCCGCATCCGGCAATCAATGCCAGCTGCGACGCTGTCTCGTAATATAAGGCCTACAACTGTTGCCg                                                                                                                                                 >  2:391807/1‑150 (MQ=11)
    ggCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGCCCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTTTAAGGCCTACAACTGTTGCCgg                                                                                                                                                >  2:1583523/1‑149 (MQ=12)
              gCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGcc                                                                                                                                      >  1:621002/1‑150 (MQ=34)
                cTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCgg                                                                                                                                    <  2:1041061/150‑1 (MQ=34)
                cTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCgg                                                                                                                                    <  2:1223297/150‑1 (MQ=34)
                 tGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGa                                                                                                                                   <  1:682115/150‑1 (MQ=34)
                  gATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTAGAGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGAt                                                                                                                                  >  2:651017/1‑150 (MQ=21)
                   aTGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATa                                                                                                                                 <  2:154677/150‑1 (MQ=34)
                     gCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAg                                                                                                                               <  2:663365/150‑1 (MQ=34)
                      cGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAgg                                                                                                                              <  1:630150/150‑1 (MQ=34)
                      cGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAgg                                                                                                                              >  2:1755167/1‑150 (MQ=17)
                       gACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGc                                                                                                                             <  1:53255/150‑1 (MQ=34)
                       gACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGc                                                                                                                             <  1:1210241/150‑1 (MQ=34)
                        aCGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCa                                                                                                                            <  2:1546290/150‑1 (MQ=35)
                           cTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACtt                                                                                                                         >  1:803037/1‑150 (MQ=37)
                               cgcgTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGc                                                                                                                     >  1:553929/1‑150 (MQ=37)
                                gcgTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGcc                                                                                                                    <  1:791856/150‑1 (MQ=37)
                                 cgTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCg                                                                                                                   <  2:218110/150‑1 (MQ=37)
                                      tATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATcc                                                                                                              >  1:1551420/1‑150 (MQ=37)
                                       aTCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCg                                                                                                             >  1:1314079/1‑150 (MQ=37)
                                           ggCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaa                                                                                                         >  2:926064/1‑150 (MQ=37)
                                               tACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaatcaa                                                                                                     >  2:494785/1‑150 (MQ=37)
                                                 cAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATg                                                                                                   <  2:515207/150‑1 (MQ=37)
                                                 cAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATg                                                                                                   >  2:64421/1‑150 (MQ=37)
                                                 cAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATg                                                                                                   <  2:1010827/150‑1 (MQ=37)
                                                 cAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATg                                                                                                   >  2:892029/1‑150 (MQ=37)
                                                   aCTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGcc                                                                                                 <  1:494785/150‑1 (MQ=17)
                                                   aCTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTCATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGcc                                                                                                 <  1:766770/150‑1 (MQ=37)
                                                        tGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACACCGGTTGCCGACCTGTCGGCCGGATACGGCACTTGTGCCGCCTCAGGCAATCAATGCctgctg                                                                                            >  1:1188789/1‑150 (MQ=11)
                                                        tGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATg                                                                                            >  2:1643228/1‑150 (MQ=34)
                                                         gCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGc                                                                                           >  1:1858259/1‑150 (MQ=34)
                                                         gCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGc                                                                                           >  2:1754795/1‑150 (MQ=34)
                                                         gCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGc                                                                                           >  2:1712285/1‑150 (MQ=34)
                                                         gCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGAACTGTAGGCCGGATAAGGCCCTTGTGCCGCAGCCGGCAATCAATGCCTGATGc                                                                                           >  2:305695/1‑150 (MQ=255)
                                                          ccGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCg                                                                                          >  1:766207/1‑150 (MQ=34)
                                                              ccTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGc                                                                                      >  2:1807925/1‑150 (MQ=34)
                                                               cTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCt                                                                                     >  2:518995/1‑150 (MQ=34)
                                                                tGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTg                                                                                    >  2:445637/1‑150 (MQ=34)
                                                                tGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACAGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATCCCAGAAGCGACGCTg                                                                                    >  2:637742/1‑150 (MQ=255)
                                                                ggTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTg                                                                                    <  2:1353253/149‑1 (MQ=21)
                                                                 gTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCTATCATTTCATGATTCGACGATGTCGCGTCTTATCAGGCCGACACCTGTTGCCGACCTGGAGGCCGGATAAGGCACTTGGGCCGCATCCGGCACTCACTGCCTGATGCGAAGCTGt                                                                                   >  2:90308/1‑150 (MQ=11)
                                                                  tAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTc                                                                                  <  2:471463/150‑1 (MQ=34)
                                                                   aGGCCTGATAAGGCTCTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcg                                                                                 <  1:921950/150‑1 (MQ=21)
                                                                    tgCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcgc                                                                                >  1:1325729/2‑150 (MQ=21)
                                                                     gCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcgcg                                                                               <  2:1119595/150‑1 (MQ=34)
                                                                     accTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACCACTGTAGCCGACCTGTAGGCAGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcgcg                                                                               >  1:159937/2‑150 (MQ=255)
                                                                        tGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCt                                                                            <  1:597747/150‑1 (MQ=32)
                                                                        tGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCt                                                                            >  2:79862/1‑150 (MQ=32)
                                                                         gATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCtt                                                                           >  2:1606542/1‑150 (MQ=32)
                                                                          aTAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTa                                                                          <  1:1348109/150‑1 (MQ=32)
                                                                          aTAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTa                                                                          <  1:454323/150‑1 (MQ=32)
                                                                             aGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCa                                                                       <  1:1800400/150‑1 (MQ=32)
                                                                               gCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGCCCTGTAGGCCGGAGAAGGCACTTGTGCCGCATCCGGCAATCAATGCCAGATGCGACGCTGTAGCGACTTATCAgg                                                                     >  2:1132112/1‑150 (MQ=11)
                                                                               gCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAgg                                                                     >  2:1785065/1‑150 (MQ=32)
                                                                               gCACTTGTGCCGCATACGGAAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTAGAACTGTTGCCGACCTGAAGGCCGGATAAGGCACTTGTGCCGCATCCGGCCATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAgg                                                                     <  2:866182/150‑1 (MQ=11)
                                                                               gCACTTGCGCCGCAGCCGGCAATCAATGCCTGATGCGACGCTGTCGCGGCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAgg                                                                     <  2:262017/150‑1 (MQ=12)
                                                                                cACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGc                                                                    >  2:668357/1‑150 (MQ=32)
                                                                                cACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGc                                                                    >  2:1671592/1‑150 (MQ=32)
                                                                                 aCTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGcc                                                                   <  2:553929/150‑1 (MQ=32)
                                                                                  cTTGTGCCGCATCCGGCAATCAATGCCCGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCt                                                                  <  2:1087799/150‑1 (MQ=17)
                                                                                     gcgCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACa                                                               <  2:1005318/148‑1 (MQ=17)
                                                                                       gCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTGTTATCAGGCCTTCTACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAc                                                             <  1:482240/150‑1 (MQ=12)
                                                                                       gCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAc                                                             >  1:487131/1‑150 (MQ=32)
                                                                                        ccGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCGACAACt                                                            >  1:191470/1‑150 (MQ=17)
                                                                                         cGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCGACAACTa                                                           >  1:1109200/1‑150 (MQ=17)
                                                                                            aTCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTg                                                        <  2:1557085/150‑1 (MQ=34)
                                                                                            aTCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTg                                                        <  2:1341814/150‑1 (MQ=34)
                                                                                              ccGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGcc                                                      <  1:1490519/150‑1 (MQ=34)
                                                                                               cGGCAATCACTGCCTGATGAGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGACTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCg                                                     <  2:1435855/150‑1 (MQ=255)
                                                                                               cGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCGACAACTATTGCCg                                                     >  1:22569/1‑150 (MQ=21)
                                                                                                      caatGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTa                                              <  2:1552683/150‑1 (MQ=37)
                                                                                                         tGCCTGATGCGACGCTGTCGCGTCTTATCAGGGCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACACCTATTGCCGACCTGTAGGc                                           >  1:527579/1‑150 (MQ=255)
                                                                                                           ccTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCg                                         >  1:720275/1‑150 (MQ=37)
                                                                                                           ccTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCg                                         >  2:641014/1‑150 (MQ=17)
                                                                                                            cTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCgg                                        <  1:641014/150‑1 (MQ=37)
                                                                                                             tGATGCGACGCTGTCGCGTCTTATCATGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATTCCAGATGCGACGCTGTCGAGTATTTTAAGGCCAACAACTATTGCCGAGCTGTATGCCGGa                                       >  2:1660129/1‑150 (MQ=11)
                                                                                                              gATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGAt                                      <  1:59037/150‑1 (MQ=37)
                                                                                                                tGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATaa                                    <  2:803037/150‑1 (MQ=37)
                                                                                                                 gCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTAACAGGCCTACAACTATTGCCGACCTGTAGGCCGGATTAg                                   >  2:646735/1‑150 (MQ=255)
                                                                                                                  cGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATTCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAgg                                  <  2:1858259/150‑1 (MQ=37)
                                                                                                                   gACGCTGTCGCGTCTTATCAGGCCTACAATTTTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGAGGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGc                                 <  1:260802/150‑1 (MQ=255)
                                                                                                                   gACGCTGTAGCGTATTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGAGCCGCATCCGTCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGc                                 <  2:1743706/150‑1 (MQ=255)
                                                                                                                          tCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTtgtg                          <  2:586471/150‑1 (MQ=37)
                                                                                                                          tCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTtgtg                          <  2:741401/150‑1 (MQ=17)
                                                                                                                              gTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGc                      <  1:127162/150‑1 (MQ=37)
                                                                                                                                  tATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATcc                  <  1:469184/150‑1 (MQ=37)
                                                                                                                                     cAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGc               >  2:1803284/1‑150 (MQ=37)
                                                                                                                                      aGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCCGGCCTTCACCTATTGACGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGca              >  2:1567672/1‑150 (MQ=255)
                                                                                                                                      aGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGca              <  1:1754795/150‑1 (MQ=37)
                                                                                                                                       ggCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaa             >  1:857648/1‑150 (MQ=37)
                                                                                                                                        gCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaat            <  2:748566/150‑1 (MQ=17)
                                                                                                                                         ccTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaatc           >  2:1813855/1‑150 (MQ=37)
                                                                                                                                         ccTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTACCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaatc           <  1:1044264/150‑1 (MQ=17)
                                                                                                                                          cTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaatca          >  2:1535139/1‑150 (MQ=37)
                                                                                                                                               aCTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCAGTAGGCCGGATAAGGCACTTGAGCCGCATCCGGCAATCAATGcc     >  1:88848/1‑150 (MQ=255)
                                                                                                                                               aCTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGcc     <  2:1551420/150‑1 (MQ=17)
                                                                                                                                                 tGTTGCCTACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTg   <  2:134366/150‑1 (MQ=25)
                                                                                                                                                 tGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTg   <  1:843109/150‑1 (MQ=35)
                                                                                                                                                  gTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGa  <  2:720275/150‑1 (MQ=35)
                                                                                                                                                  |                                                                                                                                                     
ATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGA  >  NC_000913/4094343‑4094638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: