Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,106,371 C→A 5.7% intergenic (+51/‑98) cpxP → / → fieF inhibitor of the cpx response; periplasmic adaptor protein/ferrous iron and zinc transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,106,3710CA 5.7% 293.8 / 4.1 109intergenic (+51/‑98)cpxP/fieFinhibitor of the cpx response; periplasmic adaptor protein/ferrous iron and zinc transporter
Reads supporting (aligned to +/- strand):  ref base C (50/52);  new base A (4/3);  total (54/55)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

AACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGG  >  NC_000913/4106229‑4106520
                                                                                                                                              |                                                                                                                                                     
aacaacGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAATCCCTGTTTTCCTTGCCATAGAAACCATCCCTGTCTTCCCCACCATGCTGTgggg                                                                                                                                                >  2:144379/1‑150 (MQ=255)
 acaacGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGCAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTTCCATAGACACGATCCCTGTCTTCCCCCACATGCTGgggggg                                                                                                                                               >  2:124817/1‑150 (MQ=255)
  caacGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCGGTGGGGGt                                                                                                                                              >  2:1542206/1‑150 (MQ=255)
   aacGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGtt                                                                                                                                             <  2:842397/150‑1 (MQ=255)
    acGACTGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACAGCATGTgggggaag                                                                                                                                            <  1:965234/150‑4 (MQ=255)
    acGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCACCTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGttt                                                                                                                                            >  2:1644668/1‑150 (MQ=255)
    acGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACAGCATGTgggggaag                                                                                                                                            <  1:1447305/150‑4 (MQ=255)
     cGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGAAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACAGGCGGGGGGGGgttt                                                                                                                                           >  1:319859/1‑150 (MQ=255)
      gAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGttttt                                                                                                                                          >  1:269866/1‑150 (MQ=255)
       aaTGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGtttttt                                                                                                                                         <  2:43749/150‑1 (MQ=255)
       aaTGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGtttttt                                                                                                                                         <  1:1599995/150‑1 (MQ=255)
        aTGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGttttttt                                                                                                                                        <  1:1021320/150‑1 (MQ=255)
        aTGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGttttttt                                                                                                                                        <  2:35684/150‑1 (MQ=255)
        aTGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACAGCATGTgggggaagacag                                                                                                                                        <  1:43758/150‑8 (MQ=255)
          tgtgCATTTGCGTGACGTGACGCAATGGGAATAAAGTTCATCGTTGAAGCTATTTAGTAGTAGCAACTCTCGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTa                                                                                                                                      <  1:1398427/147‑1 (MQ=255)
          ggAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTa                                                                                                                                      <  2:331276/150‑1 (MQ=255)
           gAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTAt                                                                                                                                     <  1:1073476/150‑1 (MQ=255)
            aGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATc                                                                                                                                    >  2:761037/1‑150 (MQ=255)
             gCATTTGCGTGACGTGACGCATTGGCAAAAAATTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTTATCCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATcc                                                                                                                                   <  1:268581/150‑1 (MQ=255)
               aGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCCTCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGGTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACCTGCTGTGGGGGTTTTTTTTATCCTc                                                                                                                                 <  1:1681528/150‑1 (MQ=255)
                     gtgacgtgacGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTg                                                                                                                           >  1:573944/1‑150 (MQ=255)
                            gacgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCctgctg                                                                                                                    >  1:1528931/1‑150 (MQ=255)
                             acgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCtgctgc                                                                                                                   <  1:1608805/150‑1 (MQ=255)
                             acgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCtgctgc                                                                                                                   <  1:1601193/150‑1 (MQ=255)
                             acgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCtgctgc                                                                                                                   <  2:802031/150‑1 (MQ=255)
                             acgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCtgctgc                                                                                                                   <  1:1203904/150‑1 (MQ=255)
                             acgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCtgctgc                                                                                                                   <  1:285710/150‑1 (MQ=255)
                               gCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCtt                                                                                                                 <  2:1530527/150‑1 (MQ=255)
                                  aTGGCAAAAAATTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAAt                                                                                                              <  2:520791/150‑1 (MQ=255)
                                   aggCAAATAATATCAGCGTTGAAGCTATTGAGTACTCGCCACTCCCGCTCCCACTACTAAACCCTGTATTCCATCCCAACGACACCATCCCTGTCTTCCCCCCCCTGCTGTGGTGGTTTTTTTTATCCTCAATTGGCCTGCTGCTTAATg                                                                                                             <  2:324739/149‑1 (MQ=255)
                                      cAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCAt                                                                                                          >  2:793130/1‑150 (MQ=255)
                                      cAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCAt                                                                                                          >  2:809318/1‑150 (MQ=255)
                                      cAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGGTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCAt                                                                                                          >  2:1560379/1‑150 (MQ=255)
                                      cAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGATGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCAt                                                                                                          >  2:666175/1‑150 (MQ=255)
                                        aaaaaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTg                                                                                                        >  1:652588/1‑150 (MQ=255)
                                        aaaaaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTg                                                                                                        <  1:51863/150‑1 (MQ=255)
                                        aaaaaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACAATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAAAGCTTTg                                                                                                        >  1:357386/1‑150 (MQ=255)
                                         aaaaGTTCATCGTTGAAGCTATTGAGTAGTAGCTACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGc                                                                                                       <  1:958514/150‑1 (MQ=255)
                                           aaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAg                                                                                                     <  1:902271/150‑1 (MQ=255)
                                            aGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCCTCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCATTTTGCCTGCTGCTTAATGCATTGCTga                                                                                                    >  2:1202516/1‑150 (MQ=255)
                                             tttCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAgat                                                                                                   <  1:1282050/149‑1 (MQ=255)
                                                  tCGTTGAAGCAATAGAGTAGTAGCAACTCACGTTCCCAGTAGTACACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTACCCCACATGCTGTGGGGGTTTTTTTTAACCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGAttt                                                                                              <  2:342014/150‑1 (MQ=255)
                                                   ggTTGAAGCAATTGAGAAGTAGCTACTCACGTTGCCAGTAGTAAACCGTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCGTATTGCATTGCAGATGATTTg                                                                                             <  2:568258/149‑1 (MQ=255)
                                                     ttGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCt                                                                                           <  1:77764/150‑1 (MQ=255)
                                                      tGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGGTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCtt                                                                                          >  2:1656844/1‑150 (MQ=255)
                                                       gAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTACCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGAAGATTATTTGCCTc                                                                                         >  2:1435778/1‑150 (MQ=255)
                                                          gCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGt                                                                                      <  1:1092995/150‑1 (MQ=255)
                                                          gCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGt                                                                                      >  1:308206/1‑150 (MQ=255)
                                                            tATTGAGTAGTAGCAACTCTCGTTCCCAGTAGTAACCCCTGATTTGCTTGCCATAGACACCATCCCTGTCTTCCACCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGGAGATGATTTGCTTCCGttt                                                                                    >  2:936673/1‑149 (MQ=255)
                                                            tATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTta                                                                                    <  1:1709330/150‑1 (MQ=255)
                                                            tATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTta                                                                                    <  2:964638/150‑1 (MQ=255)
                                                            tATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGGTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGttt                                                                                    >  2:592953/1‑149 (MQ=255)
                                                             aTTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACCTGCGGTGGGGGGTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATTATTTGCTTCCGTtat                                                                                   >  1:1386069/1‑150 (MQ=255)
                                                             aTTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTtat                                                                                   >  2:1072391/1‑150 (MQ=255)
                                                             aTTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTtat                                                                                   <  1:733649/150‑1 (MQ=255)
                                                              ttGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGTTTTGCTTCCGTtatt                                                                                  >  2:1330346/1‑149 (MQ=255)
                                                              ttGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTtata                                                                                  >  1:1141106/1‑150 (MQ=255)
                                                                gAGTAGTAGCAACTCCCGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGGGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACt                                                                                >  1:826394/1‑150 (MQ=255)
                                                                  gtagtaGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCATGTCTTCCCCCACATGCTGTGGGGGTTTATTTTATCCTCAATTTGCCTGAGGGTTAAAGCATTGCAGATGATTTGCTTCAGTTATACTAg                                                                              >  2:1587869/1‑150 (MQ=255)
                                                                    agtagCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCg                                                                            <  1:1487667/150‑1 (MQ=255)
                                                                    agtagCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGGCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCg                                                                            <  1:1480166/150‑1 (MQ=255)
                                                                     gtagCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTTATGCATTGCAGATGATTTGCTTCCGTTATACTAGCgg                                                                           >  1:1730777/1‑149 (MQ=255)
                                                                       agCAACTCACGTTCCCAGTAGTAAACCCTGGTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCGGTGGGGGTTTTTTTTATCCTCAATTTGCGTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCa                                                                         >  2:226635/1‑150 (MQ=255)
                                                                          aaCTCACGTTCCCAGTATTAAACCCTGTTTTCCGTGCCATAGCCACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGtt                                                                      <  2:1401206/150‑1 (MQ=255)
                                                                          aaCTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCCTCCCTGTCTTCCCCCACATGCTTTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCTGATGATTTGCTTCCGTTATACTAGCGTCAGtt                                                                      <  1:1841978/150‑1 (MQ=255)
                                                                            cTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGa                                                                    <  1:1707506/150‑1 (MQ=255)
                                                                              cACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATACCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATa                                                                  <  2:653819/150‑1 (MQ=255)
                                                                                  ttCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCgg                                                              <  2:1261365/150‑1 (MQ=255)
                                                                                   tCCCAGTAGTAACCCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCggg                                                             <  2:269866/150‑1 (MQ=255)
                                                                                   tCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCggg                                                             <  2:1655092/150‑1 (MQ=255)
                                                                                    cccAGTAGTAAACCCTGTTTTACTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGa                                                            <  1:144379/150‑1 (MQ=255)
                                                                                     ccAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCCCATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGGTGCTTAATGCATTGCAGATGATTTGCTTCCGGTTTAATAGGGTCAGTTGATAGCGGGAg                                                           >  2:1183885/1‑150 (MQ=255)
                                                                                            taAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTAt                                                    >  2:970872/1‑150 (MQ=255)
                                                                                               aCCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCACTTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGaa                                                 <  2:1636840/150‑1 (MQ=255)
                                                                                                cccTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGaat                                                >  2:1507960/1‑150 (MQ=255)
                                                                                                cccTGTTTTCCTTGCCATAGACACCATCCCTGTCTTACCCCACCAGCTGTGGGGGTTTTTTTTATCCTCAAATTGCATGAATCTAAATGCATTGCAGATGATTTGCGTACGATATACTAGCGTAAATTGATAGCCGGAGTATTTATGaat                                                >  1:1372876/1‑150 (MQ=255)
                                                                                                  cTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGaatca                                              >  2:9436/1‑150 (MQ=255)
                                                                                                   tGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTTTGaatcaa                                             >  1:531886/1‑150 (MQ=255)
                                                                                                   tGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGaatcaa                                             <  1:433054/150‑1 (MQ=255)
                                                                                                      tttCCTTGCCATCCACACCTTCCCTGTGTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCt                                          <  1:1301557/150‑1 (MQ=255)
                                                                                                       ttCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCtt                                         <  2:43302/150‑1 (MQ=255)
                                                                                                         ccTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTAt                                       >  2:686158/1‑150 (MQ=255)
                                                                                                         ccTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTAt                                       <  2:681295/150‑1 (MQ=255)
                                                                                                          cTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTCGGGGTTTTTTTTAACCTCAATTAGCCTGCTGCTTAATGCATTGCAGATGATTTTCTTCCGTTATACTAGCGTCAGTTGATAGCGGCACTATTTATGATTCAATCTTATg                                      >  1:332557/1‑150 (MQ=255)
                                                                                                             gCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGAc                                   <  2:1375089/150‑1 (MQ=255)
                                                                                                               cATAGACACCATCCCTGTCTTCCCCCACATGCGGTGGGGGTTTTTTTTTTCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTTTACTAGCGTCAGTTGATTGCGGGAGTATTTATTAATCAATCTTTTGGACgg                                 >  1:1681774/1‑150 (MQ=255)
                                                                                                                   gccacCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTgg                             >  2:1723585/3‑150 (MQ=255)
                                                                                                                     cacGATCCCTGTCTTCCCGCACCTGCTGTGGGGGGTTTTTTTATCCTCAATTTTCCTGCTGCTTAATTCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGGTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTc                           <  1:774448/150‑1 (MQ=255)
                                                                                                                     cacCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTc                           <  1:1458391/150‑1 (MQ=255)
                                                                                                                                tCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGggcggc                >  2:50963/1‑150 (MQ=255)
                                                                                                                                  ttCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGGTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGa              <  1:1286295/150‑1 (MQ=255)
                                                                                                                                   tCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGAt             >  1:515694/1‑150 (MQ=255)
                                                                                                                                   acccccACAGCATGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGAt             >  1:852004/2‑150 (MQ=255)
                                                                                                                                   acccccACAGCATGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGAt             >  1:1871589/2‑150 (MQ=255)
                                                                                                                                     ccccACAGCATGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGATACAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATtg           >  2:596568/1‑150 (MQ=255)
                                                                                                                                      cccACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATtgc          >  1:525007/1‑150 (MQ=255)
                                                                                                                                      cccACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATtgc          >  1:1095894/1‑150 (MQ=255)
                                                                                                                                       ccACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATtgct         >  1:850206/1‑150 (MQ=255)
                                                                                                                                              cTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACgg  <  1:1644857/150‑1 (MQ=255)
                                                                                                                                              cTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACgg  >  1:1252497/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                     
AACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGG  >  NC_000913/4106229‑4106520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: