Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,295,955 G→A 7.1% intergenic (+161/+481) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,295,9550GA 7.1% 155.5 / 4.7 71intergenic (+161/+481)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base G (33/32);  new base A (2/3);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

GGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAG  >  NC_000913/4295809‑4296102
                                                                                                                                                  |                                                                                                                                                   
ggATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGa                                                                                                                                                  <  2:591754/150‑1 (MQ=255)
ggATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGa                                                                                                                                                  <  2:599582/150‑1 (MQ=255)
 gATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGAc                                                                                                                                                 <  1:590884/150‑1 (MQ=255)
             gCATTGTGTGTATGCCTGATGCGACGCTTGCCGCGTCTTCTCAGGCCTCAGCCAGACAGCGTAATAGCCTGATTTAGCGTGATTTTGTAGGTGGGATCAGGCGTTTAAGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTc                                                                                                                                     <  1:286187/150‑1 (MQ=11)
             gCAATGTGTGAATGCCTGTTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAGTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTc                                                                                                                                     <  1:352398/150‑1 (MQ=255)
             gCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTc                                                                                                                                     <  1:357738/150‑1 (MQ=40)
              cAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCt                                                                                                                                    <  2:1609462/150‑1 (MQ=39)
                   tgtgAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGGTTAACTCGTCTTATct                                                                                                                               >  1:1226687/1‑149 (MQ=25)
                   tgtgAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTTTCa                                                                                                                               >  1:1157200/1‑150 (MQ=25)
                   tgtgAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCa                                                                                                                               <  1:1828341/150‑1 (MQ=35)
                    gtgAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATTGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAg                                                                                                                              <  2:1196417/150‑1 (MQ=21)
                    gtgAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGATTATGCCGCCAACGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAg                                                                                                                              >  2:1095741/1‑150 (MQ=255)
                     tgAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAgg                                                                                                                             <  1:1309685/150‑1 (MQ=33)
                      gAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                            <  2:733427/150‑1 (MQ=32)
                      gAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATAAGACGCGTTAAGCGTCGCATCAGGc                                                                                                                            <  1:1087934/150‑1 (MQ=11)
                        aTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATAAGACGCGTTAAGCGTCGCATCAGGcgt                                                                                                                          <  1:499389/150‑3 (MQ=11)
                        aTGCCTGATGCGACGCTGGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCTGATATGGCGTTTATGCCGCATCCGACATCAACGCCTGATAAGACGCGTTAAGCGTCGCATCAGGcgt                                                                                                                          <  1:1202480/150‑3 (MQ=11)
                                                                                      ttAGCGTGATTTTGTATGTCGGATTTGGCGTTTATTCCGCATCCGTCATCAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAcgc                                                            <  1:421735/150‑1 (MQ=11)
                                                                                      ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAcgc                                                            <  1:572460/150‑1 (MQ=31)
                                                                                       tAGCGTGATTTTGTAGTGCTTATACGGCGTTTATGCCGCTTCCGTCTTCAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAcgcc                                                           <  2:1443498/150‑1 (MQ=11)
                                                                                       tAGCGTGATTTTGTAGGTCTGATAAGGGGTTTAAGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAcgcc                                                           <  1:493128/150‑1 (MQ=12)
                                                                                       tAGCGTGATTTTGTAGGGCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAcgcc                                                           <  1:1266309/150‑1 (MQ=17)
                                                                                        aGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGACTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAcgccg                                                          >  2:393402/1‑150 (MQ=17)
                                                                                         gCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAcgccgc                                                         >  2:577517/1‑150 (MQ=32)
                                                                                           gTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCAt                                                       >  2:1776909/1‑150 (MQ=17)
                                                                                              aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCg                                                    >  1:1401226/1‑150 (MQ=32)
                                                                                               ttttGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGa                                                   >  1:460791/1‑150 (MQ=32)
                                                                                                  tGTAGCTCGGATAAGGCTTTTATGCCTCATCCGACTTTAACGACTTATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACAt                                                <  2:1322842/150‑1 (MQ=11)
                                                                                                      ggTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaat                                            >  2:1795422/1‑149 (MQ=17)
                                                                                                       gTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatg                                           >  2:999896/1‑148 (MQ=17)
                                                                                                       gTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatg                                           >  1:761957/1‑148 (MQ=17)
                                                                                                       atCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAAGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTATGTCGGATAAGGCGATTACGACGCATCCGACATCaaag                                           >  1:1317796/2‑148 (MQ=11)
                                                                                                        tCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGc                                          >  2:819976/1‑150 (MQ=17)
                                                                                                          ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt                                        >  1:1819015/1‑150 (MQ=17)
                                                                                                           gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCATAAGCCTGATATAGCGTGAATTAGTAGATCGGATGAGGCGTTTACGCCGCATCGGCAATCAATGCCTg                                       >  2:213066/1‑150 (MQ=11)
                                                                                                            ttAAGGCGTTTATGCCGCATCAGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGa                                      <  2:1048347/149‑1 (MQ=17)
                                                                                                            aaaaGGCGTTTATGCCGCATCCGAAATCAACGCCTGATGCGACGCTTCACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGa                                      <  2:1018339/148‑1 (MQ=12)
                                                                                                               aGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc                                   >  2:1802797/1‑150 (MQ=17)
                                                                                                                ggCGTTTATGCCGCTTCCGACATGAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCg                                  <  2:441004/150‑1 (MQ=17)
                                                                                                                        tGCCGCATCCGACATCAACGCCTGATGCGACGCTTCCCGCGTCCTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGc                          <  2:1285352/150‑1 (MQ=12)
                                                                                                                         gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGcc                         >  1:1648679/1‑150 (MQ=21)
                                                                                                                         gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGACTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTAACGACGTATCCGAAATAAGCACCAGATGCGTCGCtggaa                         >  2:1143697/1‑146 (MQ=11)
                                                                                                                           cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgc                       >  2:1414944/1‑150 (MQ=25)
                                                                                                                           cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGACTGATGCGACGCTTGCcgc                       >  2:1422003/1‑150 (MQ=25)
                                                                                                                            gCATCCGTCATCAACGCCTGATGCGACGCTTCACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATATCCTGATTTAGCGTGATTTTTTAGGTCTGTTAAGGCTTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcgcg                      <  2:1233966/150‑1 (MQ=11)
                                                                                                                              aTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc                    >  2:787354/1‑150 (MQ=25)
                                                                                                                              aTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc                    <  1:518730/150‑1 (MQ=25)
                                                                                                                                ccGACATCAACGCCTGATGCGACGCTTCACGCGTCTTATCAGGCCTACGCCAGACATCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCtt                  <  2:4905/150‑1 (MQ=255)
                                                                                                                                    cATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCGAGACGGCGCTATAGCGTGATTTAGCGTGAGTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGCCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCa              >  1:165800/1‑150 (MQ=11)
                                                                                                                                      tCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg            >  1:1758838/1‑150 (MQ=37)
                                                                                                                                       cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc           >  1:1073076/1‑150 (MQ=25)
                                                                                                                                       cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAAAAAGCGTGAATATCTAGGTCGGATAAGGCGTTTACGCCGCATCCCACATCAACGCCAGATGCGACGCTTGACGCGTCTTATCAgcc           >  2:1166996/1‑148 (MQ=11)
                                                                                                                                       cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGACAGACAGCGCAATAGCCTGATTTAGCGTGATAAAGTAGTTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGATTGCAGCGTCTTAACAGGc           >  2:1201070/1‑150 (MQ=11)
                                                                                                                                        aatgCCTGATGCTACGCTTAACTGGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATATGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc          <  2:1492210/147‑1 (MQ=11)
                                                                                                                                        aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc          >  2:966218/1‑150 (MQ=25)
                                                                                                                                        aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc          <  1:393402/150‑1 (MQ=25)
                                                                                                                                          ggCCTGATAAGACGCGTTAAGCGTCGCATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa        >  1:1851177/2‑150 (MQ=11)
                                                                                                                                          cGCCTGATTCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACTGCTCAATTGCCTGATTTAGCGTGATTTTGTAGGTCGGTTAAGGCGTTTACGCCGCATCCGTCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa        <  1:578883/150‑1 (MQ=11)
                                                                                                                                           gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat       >  1:965988/1‑149 (MQ=21)
                                                                                                                                            ccTGATAAGACGCGTTAAGCGTCGCATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatc      >  2:233762/1‑148 (MQ=11)
                                                                                                                                             cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct     <  1:1467471/150‑4 (MQ=17)
                                                                                                                                             cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct     <  2:1607848/150‑4 (MQ=17)
                                                                                                                                              tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt                                        >  2:414467/1‑114 (MQ=17)
                                                                                                                                              tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt                                        <  1:414467/114‑1 (MQ=17)
                                                                                                                                              tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctt    >  2:648678/1‑146 (MQ=17)
                                                                                                                                              tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctt    >  2:1483709/1‑146 (MQ=17)
                                                                                                                                              tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGcc    <  1:1850236/150‑1 (MQ=37)
                                                                                                                                               gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctta   >  2:1407846/1‑145 (MQ=9)
                                                                                                                                               gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctta   >  2:243921/1‑145 (MQ=9)
                                                                                                                                                aTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAg  <  1:1870132/150‑1 (MQ=17)
                                                                                                                                                aTGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAg  <  1:1631514/150‑1 (MQ=37)
                                                                                                                                                  |                                                                                                                                                   
GGATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAG  >  NC_000913/4295809‑4296102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: