Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 569,160 G→T 5.6% D87Y (GAC→TAC)  ybcK → DLP12 prophage; putative recombinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913569,1600GT 5.6% 381.1 / 6.7 137D87Y (GAC→TAC) ybcKDLP12 prophage; putative recombinase
Reads supporting (aligned to +/- strand):  ref base G (62/66);  new base T (4/5);  total (66/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

TTATTATCTTGATACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCATTAATAAAAGCCATACT  >  NC_000913/569012‑569308
                                                                                                                                                    |                                                                                                                                                    
ttattaTCTTGATACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGa                                                                                                                                                     <  2:1720571/150‑1 (MQ=255)
    tatCTTGATACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGa                                                                                                                                                 <  1:1694115/150‑1 (MQ=255)
    tatCTTGATACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGa                                                                                                                                                 <  1:1482331/150‑1 (MQ=255)
     atCTTGATACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATAGCCAGGAACTAAATTGTTAGTTGAAAGTATTGACAGAc                                                                                                                                                >  1:1314408/1‑150 (MQ=255)
      tCTTGATACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCTCAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACt                                                                                                                                               >  1:1756491/1‑150 (MQ=255)
       cTTGATACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACtt                                                                                                                                              >  2:1875814/1‑150 (MQ=255)
        ttGATACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACttt                                                                                                                                             >  2:160531/1‑150 (MQ=255)
        ttGATACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACttt                                                                                                                                             >  1:1510279/1‑150 (MQ=255)
         tGATAGCATAACATATTAAGATATAGTATTAATAGCATTCAAACGATATCTTTCACAATCAGGAGCGTTGTCGGAAGTTTTAGATGCTAAAGAGCATGGTTATATATTTCCAGGAACTACATTGTTAGTTGAAAGTATGTATAGACTTTc                                                                                                                                            <  2:884041/150‑1 (MQ=255)
         tGATACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTc                                                                                                                                            <  1:1064475/150‑1 (MQ=255)
         tGATACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTc                                                                                                                                            >  2:779296/1‑150 (MQ=255)
          gATAGCATCTCATATGCTGAGTGAGGTTTTAGTGCATTCATAGGAAAGCATCCACAATCAGGACCGTTTTCGGAAATTTTAGATGCTATAGAGCATGGTTATATATTGCCAGTAACTACATTGTTAGTAGAAAGTCTGGACAGACTTTCa                                                                                                                                           <  2:941688/150‑1 (MQ=255)
            tACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCTGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCTAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaac                                                                                                                                         <  2:300142/150‑2 (MQ=255)
            tACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAag                                                                                                                                         <  1:709957/150‑1 (MQ=255)
              ccATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaacta                                                                                                                                       <  1:255458/150‑4 (MQ=255)
              ccATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAagag                                                                                                                                       <  1:1253576/150‑1 (MQ=255)
                        ggAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGATTTTTCGGAATTTTTAGATGATATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCgg                                                                                                                             >  2:1494500/2‑150 (MQ=255)
                         taaGATTTATGATTAAGTGCATTCAAATGAAAGCATGCACAATCAGGATCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGt                                                                                                                            <  2:90359/149‑1 (MQ=255)
                         taaGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGt                                                                                                                            <  1:836672/149‑1 (MQ=255)
                         gAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGt                                                                                                                            >  2:448334/1‑150 (MQ=255)
                          aaGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTg                                                                                                                           >  2:904424/1‑150 (MQ=255)
                           aGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCTGGAGCTTTGTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCGAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGa                                                                                                                          >  2:352907/1‑150 (MQ=255)
                            gATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGTGAAAAAGTCGGTGaa                                                                                                                         >  1:345966/1‑150 (MQ=255)
                            gATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGaa                                                                                                                         >  2:1081055/1‑150 (MQ=255)
                                  ggtttAAGTGCATTCAAAGGAAAGCATGCACAATCAGGCGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGAtt                                                                                                                   <  1:590105/147‑1 (MQ=255)
                                   taTTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTg                                                                                                                  <  2:380125/149‑1 (MQ=255)
                                   taTTAAGTGCATTCAAAGGAAAGAATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTg                                                                                                                  <  2:27212/149‑1 (MQ=255)
                                         ttGCATTTAAACGAAAGCATTCACAAATATGTTCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTc                                                                                                            <  2:72350/149‑1 (MQ=255)
                                         gTGCATTCTAATGAAAGCTTGCACAATCAGGAGCTTTTTCGGATTTTTTAGATGATATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTc                                                                                                            <  1:1494777/150‑1 (MQ=255)
                                         gTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTc                                                                                                            >  1:863379/1‑150 (MQ=255)
                                          tGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGCTGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCTGTGAAGCGATTGAACGTCt                                                                                                           >  1:1006390/1‑150 (MQ=255)
                                          tGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCt                                                                                                           >  1:563932/1‑150 (MQ=255)
                                          tGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGGGAAGCGATTGAACGTCt                                                                                                           <  2:1331736/150‑1 (MQ=255)
                                              ttCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGaaa                                                                                                       >  1:1743725/1‑150 (MQ=255)
                                               tCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAAt                                                                                                      >  2:1415583/1‑150 (MQ=255)
                                                cAAAGGAAAGCATGCACAATCAGGAGATTTTTCGGAATATTTAGATGCTAAAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGTTGAAGCTATTGAACTTCTGAAAtt                                                                                                     >  1:143823/1‑150 (MQ=255)
                                                 aaaGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTg                                                                                                    >  1:1714662/1‑150 (MQ=255)
                                                 aaaGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTg                                                                                                    <  2:360316/150‑1 (MQ=255)
                                                    ggAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGAtt                                                                                                 >  1:920514/1‑150 (MQ=255)
                                                         gCATTCACAATCAGGAGCTTTTTCGGAATTTTTATATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGaa                                                                                            <  1:1209692/150‑1 (MQ=255)
                                                         gCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGaa                                                                                            <  1:485394/150‑1 (MQ=255)
                                                           aTGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGTAGAAGCGATTGAACGTCTGAAATTGAATTTGAATc                                                                                          >  1:1234667/1‑150 (MQ=255)
                                                            tGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCa                                                                                         >  2:667303/1‑150 (MQ=255)
                                                                 aaTCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTa                                                                                    >  1:1042399/1‑150 (MQ=255)
                                                                  aTCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTAt                                                                                   >  2:1305139/1‑150 (MQ=255)
                                                                    cAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTg                                                                                 <  2:1169962/150‑1 (MQ=255)
                                                                     aGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGa                                                                                <  1:486841/150‑1 (MQ=255)
                                                                     aGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGa                                                                                >  1:238939/1‑150 (MQ=255)
                                                                      ggAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGAt                                                                               >  1:678062/1‑150 (MQ=255)
                                                                         gCTTTTTCGGAATTTTTAGATGCTATAGAGCATGTTTATATATTGCCAGGAACTACATTGTTATTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGtt                                                                            <  1:1121250/150‑1 (MQ=255)
                                                                            ttttCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTtata                                                                         <  2:1108332/150‑1 (MQ=255)
                                                                                 ggAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTAGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAActct                                                                    >  1:338051/1‑150 (MQ=255)
                                                                                 ggAATTTTTAGATGCTATAGAGCATGGATATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTACAGACTTTAAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTCAATCACGGTATTGATGTTATAActct                                                                    >  1:222094/1‑150 (MQ=255)
                                                                                  gAATTTTTAGATGCTAAAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGAAAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCAAGGTATTGATGATATAActcat                                                                   >  1:336712/1‑148 (MQ=255)
                                                                                   aaTTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGTAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCttt                                                                  <  2:1494108/150‑1 (MQ=255)
                                                                                   aaTTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCttt                                                                  <  1:448334/150‑1 (MQ=255)
                                                                                     tttttAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTTTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAATGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGc                                                                <  2:921811/150‑1 (MQ=255)
                                                                                     tttttAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGc                                                                <  1:18060/150‑1 (MQ=255)
                                                                                     tttttAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGc                                                                <  2:1178795/150‑1 (MQ=255)
                                                                                       tttAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGa                                                              >  2:728836/1‑150 (MQ=255)
                                                                                        ttAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGAc                                                             <  1:160531/150‑1 (MQ=255)
                                                                                        ttAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGAc                                                             <  2:1510279/150‑1 (MQ=255)
                                                                                        ttAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGAc                                                             <  2:1070412/150‑1 (MQ=255)
                                                                                             tGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATAc                                                        <  2:585955/150‑1 (MQ=255)
                                                                                             tGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATAc                                                        >  1:1547020/1‑150 (MQ=255)
                                                                                               cTACAGAGCATGGTTATATATTTCCAGGAACTACATTGTTAATTGAAACACTCGACACACTTTCAAGAGAAAAAGTCGGCGAAGCGATTGAACGTCTGAAATTGATTTAGACTCACGGTATTGATGTTAAAACTCTTTGCGACACTACAg                                                      <  2:1121608/150‑1 (MQ=255)
                                                                                                tataGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACTGTATTGATGTTATAACTCTTTGCTACAATACAGt                                                     >  1:109728/1‑150 (MQ=255)
                                                                                                 ataGAGCATGGTTTTATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTc                                                    <  1:1284830/150‑1 (MQ=255)
                                                                                                 ataGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTc                                                    <  1:1513288/150‑1 (MQ=255)
                                                                                                  taGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCt                                                   <  1:403274/150‑1 (MQ=255)
                                                                                                    gagCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCTATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCtat                                                 >  1:156741/1‑150 (MQ=255)
                                                                                                     agCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCtata                                                <  1:769465/150‑1 (MQ=255)
                                                                                                          ggTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATAtt                                           <  2:1375616/150‑1 (MQ=255)
                                                                                                           gTTATATATTGCCATTAACTACATTGTTATTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCTGTGAAGCGAATGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTg                                          <  2:1314408/150‑1 (MQ=255)
                                                                                                           gTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTg                                          >  2:1668117/1‑150 (MQ=255)
                                                                                                                atatTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGACCGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGActct                                     <  2:516043/150‑1 (MQ=255)
                                                                                                                 tatTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTAtt                                    >  1:253104/1‑150 (MQ=255)
                                                                                                                  atTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCttt                                   >  2:423971/1‑150 (MQ=255)
                                                                                                                   ttGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCtttt                                  >  2:825911/1‑149 (MQ=255)
                                                                                                                     gCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGaa                                >  1:1576780/1‑150 (MQ=255)
                                                                                                                     gCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGaa                                <  1:481878/150‑1 (MQ=255)
                                                                                                                     gCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTCCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGaa                                >  2:511293/1‑150 (MQ=255)
                                                                                                                      ccAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAAt                               >  1:17988/1‑150 (MQ=255)
                                                                                                                       cAGGAACTACATTGTTAGTTGGAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATg                              >  2:1023238/1‑150 (MQ=255)
                                                                                                                         ggAACTACATTGTTAGTTGATAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAg                            <  2:1756491/150‑1 (MQ=255)
                                                                                                                            aCTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCTGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCa                         <  1:78167/150‑1 (MQ=255)
                                                                                                                            aCTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCa                         <  1:779296/150‑1 (MQ=255)
                                                                                                                                 aTTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGACATAtta                    >  1:457679/1‑149 (MQ=255)
                                                                                                                                    tttAGTTGAAAGTCTTGACATACTTTCAAGAGAAAAAGTCGGTGAAGCTATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTATTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCAtt                 <  1:1767417/149‑1 (MQ=255)
                                                                                                                                      ctcttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCATtaa               >  1:415434/4‑150 (MQ=255)
                                                                                                                                        gTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCATtaata             >  1:1742676/1‑150 (MQ=255)
                                                                                                                                        cttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCATtaata             >  1:722154/2‑150 (MQ=255)
                                                                                                                                          tGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGATCCATATTCATTAATaaa           >  1:619793/1‑150 (MQ=255)
                                                                                                                                          tGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCATTAATaaa           <  1:914381/150‑1 (MQ=255)
                                                                                                                                           gAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCATTAATaaaa          >  2:55133/1‑150 (MQ=255)
                                                                                                                                            aaaGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCATTAATAAAAg         <  1:904424/150‑1 (MQ=255)
                                                                                                                                             aaGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCATTAATAAAAGc        <  1:664186/150‑1 (MQ=255)
                                                                                                                                              aGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCATTAATAAAAGcc       >  1:1536315/1‑150 (MQ=255)
                                                                                                                                               gTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCATTAATAAAAGCCa      <  2:78725/150‑1 (MQ=255)
                                                                                                                                                   ggACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCATTAATAAAAGCCATACt  >  1:1710402/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTATTATCTTGATACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCATTAATAAAAGCCATACT  >  NC_000913/569012‑569308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑