Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,325,948 A→G 6.0% intergenic (‑207/+451) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,325,9480AG 6.0% 202.9 / 3.5 86intergenic (‑207/+451)yjdN/yjdMmetalloprotein superfamily protein/zinc‑ribbon family protein
Reads supporting (aligned to +/- strand):  ref base A (27/50);  new base G (3/2);  total (31/55)
Fisher's exact test for biased strand distribution p-value = 3.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

AGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGG  >  NC_000913/4325799‑4326095
                                                                                                                                                     |                                                                                                                                                   
agagGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCCCTGTTGCCGGCTGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCCGTCGGCCCCa                                                                                                                                                     >  1:909937/1‑150 (MQ=255)
   ggcggcCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGGGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCCGGCGGCCCCGTTg                                                                                                                                                  >  1:1435315/1‑150 (MQ=11)
      tgcCTGATGCGACGCTCGCGCGTCTTATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCg                                                                                                                                               >  2:1303054/2‑150 (MQ=11)
      ggcCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCg                                                                                                                                               <  2:1252135/150‑1 (MQ=37)
       gcCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCgg                                                                                                                                              <  2:20521/150‑1 (MQ=37)
             tcagcagcgcgAGCGGCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTCAACGCCTTATCCGGCCGACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATgcgg                                                                                                                                        <  2:325222/144‑1 (MQ=11)
              aaGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATgcggc                                                                                                                                       >  2:1047901/1‑150 (MQ=37)
               cgACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATgcggcg                                                                                                                                      <  1:1568763/149‑1 (MQ=37)
               aGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATgcggcg                                                                                                                                      <  1:1447441/150‑1 (MQ=37)
                caCGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGCTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCCTTGCCGGATgcggcgg                                                                                                                                     >  1:332821/2‑149 (MQ=19)
                  cgcgcgAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTaa                                                                                                                                   <  2:1494361/150‑1 (MQ=37)
                  cgcgcgAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCCGCATTGCCGGAGGCGGCGGaa                                                                                                                                   >  1:1601852/1‑150 (MQ=11)
                   gcgctAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTaaa                                                                                                                                  <  1:70078/150‑1 (MQ=21)
                    cgcgAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAAc                                                                                                                                 <  2:374408/150‑1 (MQ=37)
                     gcgAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACg                                                                                                                                <  2:539328/150‑1 (MQ=37)
                      cgAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGc                                                                                                                               <  1:1207027/150‑1 (MQ=35)
                       gAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGcc                                                                                                                              <  2:190275/150‑1 (MQ=35)
                       gAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGcc                                                                                                                              <  1:1871259/150‑1 (MQ=35)
                            gcggaTCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTACACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATc                                                                                                                         <  1:355557/146‑1 (MQ=21)
                             cgcaggaGGCAGTCGGCCCGGCTGCCGGATGGGGCGTAAACGCCTTATGCGGCCTACGGAGGGTGCGGGAAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGAGGTGGCGTAAACGCCTTATcc                                                                                                                        <  1:287105/144‑1 (MQ=11)
                                 tCAGGCAGTCGGCTCTGTTGGCGGATGCGGCGTAAACGCCTTCTCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                    <  1:1219704/150‑1 (MQ=255)
                                 tCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                    <  2:1609496/150‑1 (MQ=34)
                                  cAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                                                                   <  2:290985/150‑1 (MQ=34)
                                  cAGGCAGTCGGCACTGTTGACGGAGGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                                                                   <  2:30661/150‑1 (MQ=21)
                                     gCAGTCGGCACTGTTGCCGGATGCGGCGTAAAGGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATAAGGCGTTTACGCCGCATCCGGcaatgg                                                                                                                <  2:60154/150‑6 (MQ=11)
                                      cAGGCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGGCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACgg                                                                                                               <  1:932359/150‑1 (MQ=18)
                                            gCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTg                                                                                                         <  2:1808956/150‑1 (MQ=32)
                                                ggTTGCCGGATGCGGCGTACCCGCCTTATCCGGCCGCCGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACCCGCAAGCGGCGCATCAGGCAGGCGGCACGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCggg                                                                                                     <  1:985281/149‑1 (MQ=11)
                                                   tGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAt                                                                                                  >  1:792978/1‑150 (MQ=32)
                                                   tGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCATGATAAGACGCGCAAGCGTCGCATCAGGCAGACGGCACCATTGCCGGATGCGGCGTAAACGCCTTATACGGCATACGGAGGGTGCGGGTAt                                                                                                  >  2:215943/1‑150 (MQ=11)
                                                    gCCGGATGCGGCGTAAACGCCTTCTCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAtt                                                                                                 <  1:499730/150‑1 (MQ=17)
                                                       ggttGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGt                                                                                              <  2:310751/147‑1 (MQ=17)
                                                       ggATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGt                                                                                              <  1:682621/150‑1 (MQ=32)
                                                         aTGCGGCGTTAACGCCGTATCCGGCCTACGGTGGGTGCGGGTATTAGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAACCGCCTTATCCGGCCGACGGAGGGTGCGGGAATTTGTAg                                                                                            <  2:277488/150‑1 (MQ=11)
                                                         aTGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATGTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAg                                                                                            <  1:1009514/150‑1 (MQ=17)
                                                          tGCGGCGTAAACGTCTTATGCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGACGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAgg                                                                                           <  2:55450/150‑1 (MQ=12)
                                                           gcggcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGc                                                                                          >  1:1773085/1‑150 (MQ=32)
                                                             gtcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCt                                                                                        <  2:1226481/148‑1 (MQ=17)
                                                              gcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTg                                                                                       <  2:1059847/150‑1 (MQ=32)
                                                                gTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGAt                                                                                     <  2:191864/150‑1 (MQ=32)
                                                                  aaaCGCCTTATCCGGCCGACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATaa                                                                                   <  2:701217/150‑1 (MQ=17)
                                                                           aTCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACGGTGGGTGCGGGGATTTGTAGGCGTGATAAGACgcgcga                                                                          >  1:915803/1‑148 (MQ=255)
                                                                           aTCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCaa                                                                          >  1:841349/1‑150 (MQ=32)
                                                                              ctgCCTACGGTGGTTTCTGTATTTTGTATGCCTGATATGACGCGCAAGCGTCGCATCATGCAGTCGGCACCATTGCCGGATGCGTCGTAAACGCCTTATCCGGCCTACGGAGTGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCg                                                                       <  2:595856/148‑1 (MQ=11)
                                                                              cGGCCTACTGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGGCGCATCAGGCAGTAGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCg                                                                       <  2:144269/150‑1 (MQ=12)
                                                                              cGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCg                                                                       <  2:1773085/150‑1 (MQ=32)
                                                                                gCCTCCGGATGGTGCGGGAAATCGTAGGCCTGATAAGCCGCGCCAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGTGAATTTGTAGGCCTGATAAGACGCGCAAGCGTc                                                                     <  2:909937/150‑1 (MQ=11)
                                                                                gCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTc                                                                     <  2:1835298/150‑1 (MQ=32)
                                                                                 ccTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCg                                                                    <  1:1770468/150‑1 (MQ=32)
                                                                                      ggAGTGTGCGGGATTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCa                                                               <  1:1043365/150‑1 (MQ=17)
                                                                                      ggAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACgcgc                                                                            >  1:933137/1‑137 (MQ=32)
                                                                                         gggTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGc                                                            >  2:507131/1‑150 (MQ=32)
                                                                                             gCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACAATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAGTTGTAGGCCTGATACGACGCGCAAGCGTCGCATCAGGCAGTc                                                        >  1:1406057/1‑150 (MQ=255)
                                                                                              cGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCg                                                       >  1:1064078/1‑150 (MQ=35)
                                                                                               gggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCgg                                                      >  2:1499646/1‑150 (MQ=35)
                                                                                                   agttGTAGGCCTGATCAGACGCGCACGCGTCGCATCAGGCAGCCGGCACCCTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTCCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCAcc                                                  <  2:49422/148‑1 (MQ=11)
                                                                                                    tttGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAGTAGGCACCg                                                 >  1:1155144/1‑150 (MQ=25)
                                                                                                         cggCCTGATAAGACTCGCTAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGcc                                            <  2:1174075/149‑1 (MQ=255)
                                                                                                          ggCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGCTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACAGTTGCCg                                           >  1:415700/1‑150 (MQ=255)
                                                                                                           gCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCgg                                          >  1:1529573/1‑150 (MQ=17)
                                                                                                                 tAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCt                                                                                        >  2:196245/1‑98 (MQ=33)
                                                                                                                 tAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCt                                                                                        <  1:196245/98‑1 (MQ=33)
                                                                                                                  aaGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAggg                                                                                                           >  1:662773/1‑78 (MQ=34)
                                                                                                                  aaGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATgcggc                                   <  2:613746/150‑1 (MQ=37)
                                                                                                                   aGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATgcggcg                                  <  2:1757819/150‑1 (MQ=37)
                                                                                                                    gACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCGCAGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGAGGCGGCGt                                 >  2:1230514/1‑150 (MQ=255)
                                                                                                                         gcgaGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACg                            <  1:970763/147‑1 (MQ=1)
                                                                                                                           aaGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGcc                          <  2:1490178/150‑1 (MQ=37)
                                                                                                                              cGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTa                       <  2:676380/150‑1 (MQ=37)
                                                                                                                                tCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATc                     >  1:209723/1‑150 (MQ=37)
                                                                                                                                   cATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATa                                                                                    >  2:1513717/1‑84 (MQ=33)
                                                                                                                                   cATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                  >  2:1040550/1‑150 (MQ=37)
                                                                                                                                   cATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                  >  2:69923/1‑150 (MQ=37)
                                                                                                                                     tCAGGCAGGCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTTCGGTGGGTGCTGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGcc                <  2:303716/150‑1 (MQ=255)
                                                                                                                                       cggCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa              <  1:1328569/149‑1 (MQ=17)
                                                                                                                                           aGTAGGCACCTTTGCCGGATGCGGCGTAAAAGCCTTATCCGGCCTTCGGATGGTGCGGTAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGa          <  2:235753/150‑1 (MQ=11)
                                                                                                                                            gTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACggtg         >  2:1328569/1‑148 (MQ=37)
                                                                                                                                              cGGCACTTTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATgg       <  2:1819746/150‑1 (MQ=11)
                                                                                                                                              cGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACAGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCGACGGATgg       >  1:36829/1‑150 (MQ=255)
                                                                                                                                              cGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGGGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAggg       <  1:1040550/150‑1 (MQ=17)
                                                                                                                                               ggCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAACCGCCTTATCCGGGCGACGGAGGGt      >  2:483847/1‑150 (MQ=255)
                                                                                                                                                  aCCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAgg                                                                                           <  2:243788/62‑1 (MQ=35)
                                                                                                                                                  aCCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAgg                                                                                           >  1:243788/1‑62 (MQ=35)
                                                                                                                                                   ctaTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTTCgg  <  2:653316/148‑1 (MQ=17)
                                                                                                                                                   ccATTGCCGGATGCGGCGTAAACGCCTTATCCTGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCgg  <  2:165180/150‑1 (MQ=21)
                                                                                                                                                   ccATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAg                                                                                            >  1:706137/1‑60 (MQ=35)
                                                                                                                                                   ccATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAg                                                                                            <  2:706137/60‑1 (MQ=35)
                                                                                                                                                   ccATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCgg  >  2:1249282/1‑150 (MQ=34)
                                                                                                                                                     |                                                                                                                                                   
AGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGG  >  NC_000913/4325799‑4326095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: