BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,339,802	A→G	6.3%	A240A (GCT→GCC)	adiA ←	arginine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,339,802	0	A	G	6.3%	373.6 / 11.7	126	A240A (GCT→GCC)	adiA	arginine decarboxylase
Reads supporting (aligned to +/- strand): ref base A (60/58); new base G (6/2); total (66/60)
Fisher's exact test for biased strand distribution p-value = 2.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-02

Only 100 of 127 total aligned reads displayed.

TCCTGCGGATAGATTGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGC  >  NC_000913/4339656‑4339950

tCCTGCGGATAGATTGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGcc                                                                                                                                                   <  1:1419910/150‑1 (MQ=255)

 ccTGCGGATAGATTGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCt                                                                                                                                                  >  1:753926/1‑150 (MQ=255)

  cTGCGGATAGATTGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAGGCTTg                                                                                                                                                 <  1:1809979/150‑1 (MQ=255)

   tGCGGATAGATTGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCCCGACATCGTATTCGGTCATGCAAGCCTGc                                                                                                                                                >  2:76784/1‑150 (MQ=255)

     cGGATAGATTGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATATATGGCAGTTACGGTCAACGACCACGACATCGGTATCGGTCATGCAAGCCTGCat                                                                                                                                              <  2:690568/150‑1 (MQ=255)

      ggATAGATTGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCatg                                                                                                                                             <  1:1301930/150‑1 (MQ=255)

       gATAGATTGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACAGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAAAGACCCCGACATCGTTATCGGTCATGCAAGCCTGCatga                                                                                                                                            >  2:785644/1‑150 (MQ=255)

          aGATTGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATgg                                                                                                                                         <  2:1744513/150‑1 (MQ=255)

           taTTGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATTTAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAGGCTTGCatgaccga                                                                                                                                        <  2:619538/149‑5 (MQ=255)

           gATTGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGt                                                                                                                                        >  1:1347522/1‑150 (MQ=255)

             ttGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGc                                                                                                                                      >  2:379588/1‑150 (MQ=255)

               ggCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCgg                                                                                                                                    >  2:668569/1‑150 (MQ=255)

                gCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGt                                                                                                                                   <  2:552758/150‑1 (MQ=255)

                gCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACAACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGt                                                                                                                                   >  1:1783529/1‑150 (MQ=255)

                 cccGATAATGCCGTAGCGGTTGCGGCTTGGCACCATCTAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGtt                                                                                                                                  <  1:1233803/150‑1 (MQ=255)

                 cccGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGtt                                                                                                                                  <  2:633349/150‑1 (MQ=255)

                 cccGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGtt                                                                                                                                  >  1:1495049/1‑150 (MQ=255)

                 cccGAAAATGCCGTAGCGGTTGCGGCTTGGCAACATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGtt                                                                                                                                  <  1:1674119/150‑1 (MQ=255)

                  ccGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTa                                                                                                                                 <  1:494618/150‑1 (MQ=255)

                  ccGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTa                                                                                                                                 <  1:1680636/150‑1 (MQ=255)

                  ccGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTa                                                                                                                                 >  2:841930/1‑150 (MQ=255)

                     aTAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTagag                                                                                                                              <  1:841930/150‑1 (MQ=255)

                        aTGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCg                                                                                                                           >  2:465938/1‑150 (MQ=255)

                         tGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCgg                                                                                                                          <  2:23167/150‑1 (MQ=255)

                          gCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGa                                                                                                                         <  1:988126/150‑1 (MQ=255)

                          gCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGa                                                                                                                         <  1:1028427/150‑1 (MQ=255)

                           ccGTAGCGGTTGCGGCTAGGCACCAAATAGCCCGGTATCGCGCCAGTCAGCATCAAACCTGCGTCTAACGATATATGGCAGTTACGGTCAACGACCACTCCAGCGTTATCGGTCATGCAAGCCTGAATGAAGCTGCCGTTAGAGCCGGaa                                                                                                                        >  1:1140668/1‑150 (MQ=255)

                            cGTCGCGGTTGCGGCTTGGCACCATCTAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGGCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAg                                                                                                                       <  1:1720341/150‑1 (MQ=255)

                            cGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCGACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAg                                                                                                                       >  1:1069943/1‑150 (MQ=255)

                              tAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCCTGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTa                                                                                                                     >  1:1488032/1‑150 (MQ=255)

                                   gTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACcgac                                                                                                                >  1:676799/1‑150 (MQ=255)

                                   gTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTAACGGTCATGCAAGCCTGCATGATGGTGCGGATAGAGCCGGAAGTACcgac                                                                                                                >  2:89835/1‑150 (MQ=255)

                                    ttGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACcgacg                                                                                                               <  2:1202497/150‑1 (MQ=255)

                                       cGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCTGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTTTCGGTCATGCAAGCCTGAATGATGGTGCGGTTTGAGCCGGAAGTAACGACGCCt                                                                                                            >  1:120992/1‑150 (MQ=255)

                                       cGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGCCATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACt                                                                                                            >  1:542805/1‑150 (MQ=255)

                                         gCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACcga                                                                                                                 <  2:1704016/143‑1 (MQ=255)

                                         gCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACcga                                                                                                                 >  1:1704016/1‑143 (MQ=255)

                                             ggCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGAc                                                                                                      >  2:1621449/1‑150 (MQ=255)

                                                    tataGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGc                                                                                               >  1:911634/1‑150 (MQ=255)

                                                    aataGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGc                                                                                               <  2:479975/149‑1 (MQ=255)

                                                      taGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGa                                                                                             >  1:1549283/1‑150 (MQ=255)

                                                        gcccGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATc                                                                                           <  1:1819146/148‑1 (MQ=255)

                                                         aCCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACAGCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCg                                                                                          <  2:1079439/150‑1 (MQ=255)

                                                          ccGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGCGCGATCgg                                                                                         >  1:1421199/1‑150 (MQ=255)

                                                          ccGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGAGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCgg                                                                                         >  1:1413308/1‑150 (MQ=255)

                                                             gTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCAc                                                                                      >  1:464029/1‑150 (MQ=255)

                                                             gTTTCGCGCCTGGCAGCATCAAACCTTGTTCGATGGATTTAAGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCAc                                                                                      <  1:179214/150‑1 (MQ=255)

                                                               ttCGCGCATGTCAGCATCAAACCTTGATCGATGGATTTATGGCAGTTACGGTCAACGACCCCGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGCTCGGCCCCa                                                                                    >  2:1626728/1‑150 (MQ=255)

                                                                   cgcCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATa                                                                                >  1:1492931/1‑150 (MQ=255)

                                                                   cgcCTGTCAGCATCAAACCTTGGGCGATGGATTAATGGCAGTTACGGTCACCGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGGGCGGGTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATa                                                                                <  2:890675/150‑1 (MQ=255)

                                                                    gcCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATAc                                                                               <  1:876967/150‑1 (MQ=255)

                                                                          cAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGc                                                                         <  1:714310/150‑1 (MQ=255)

                                                                           aGCATTAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCg                                                                        <  1:1063275/150‑1 (MQ=255)

                                                                           aGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCg                                                                        >  1:1403423/1‑150 (MQ=255)

                                                                            gCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGACAACGACCAAGACATCGTTATCGGTAATGCAAGACTGCATGATGGTGCGGTTAGAGACGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAAAACGCGTGCgc                                                                       >  1:412270/1‑149 (MQ=255)

                                                                             cATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGc                                                                      <  2:1352911/150‑1 (MQ=255)

                                                                              aTCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGAAATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGCGCCGGAAGTACCGCCGACTACCGACCAGGAGCGATCGGCACCAACTACGCGTGCGGCa                                                                     >  2:1433650/1‑150 (MQ=255)

                                                                                    ccTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATAtttt                                                               >  1:1572828/1‑150 (MQ=255)

                                                                                      ttGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTtttc                                                             <  1:842872/150‑1 (MQ=255)

                                                                                       tGTTCGAAGGATTTATGGCAGTTACCGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTCCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTtttcg                                                            <  1:1590841/150‑1 (MQ=255)

                                                                                        gTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTtttcgc                                                           <  2:799363/150‑1 (MQ=255)

                                                                                        gTTCGATGGATATATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGATACAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATAATtttcgc                                                           >  1:991776/1‑150 (MQ=255)

                                                                                         ttCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTtttcgct                                                          >  1:1170380/1‑150 (MQ=255)

                                                                                          tCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTtttcgctt                                                         >  2:1090213/1‑150 (MQ=255)

                                                                                          tCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTtttcgctt                                                         >  1:1042740/1‑150 (MQ=255)

                                                                                            taTGGATTTATTGCAGTTCCGGTCAACGACCACGACATCGTTATGGGTCATGCAAGCGTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGAGCGGCACCAAATACGCGTGCGGCATATTtttcgctttc                                                       <  2:1843882/149‑1 (MQ=255)

                                                                                            gATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTtttcgctttc                                                       >  1:1800906/1‑150 (MQ=255)

                                                                                             aTGGATTTATGGCAGGTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTtttcgctttcg                                                      <  1:1328130/150‑1 (MQ=255)

                                                                                              tGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTtttcgctttcgc                                                     <  1:810495/150‑1 (MQ=255)

                                                                                                gATTTATGGCAGTTACGGTCAACGACCACGACAGCGTTATCGGGCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCa                                                   <  1:377195/150‑1 (MQ=255)

                                                                                                 aTTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCaa                                                  >  1:1810471/1‑150 (MQ=255)

                                                                                                  tttATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCaaa                                                 <  1:829892/150‑1 (MQ=255)

                                                                                                  tttATGGCAGTTACGGTCAACGACCACGACATCGTTATAGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCAGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAAAACGCGTGCGGCATATTTTTCGCTTTCGCCaaa                                                 >  1:1373097/1‑150 (MQ=255)

                                                                                                              aCGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATg                                     >  1:896384/1‑150 (MQ=255)

                                                                                                               cGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATgg                                    >  2:1421575/1‑150 (MQ=255)

                                                                                                                ggTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGt                                   <  2:1394453/150‑1 (MQ=255)

                                                                                                                ggTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGt                                   <  1:4488/150‑1 (MQ=255)

                                                                                                                 gTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTc                                  >  1:776317/1‑150 (MQ=255)

                                                                                                                    aaCGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAg                               <  1:1707786/150‑1 (MQ=255)

                                                                                                                        aCCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCaaa                           >  2:633720/1‑150 (MQ=255)

                                                                                                                         ccACGACATCGTTATCGGTCATGCAAGCCTGCATGTGGGGGCGGCTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGGGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAg                          <  2:308727/150‑1 (MQ=255)

                                                                                                                         ccACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAg                          <  2:1728620/150‑1 (MQ=255)

                                                                                                                         ccACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAg                          <  2:1784149/150‑1 (MQ=255)

                                                                                                                           aCGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGaa                        >  1:924537/1‑150 (MQ=255)

                                                                                                                            cGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAAc                       <  1:1864524/150‑1 (MQ=255)

                                                                                                                             gACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAACTGCGCCAGTATGGTCAAGCAAAGAAcc                      <  2:1069943/150‑1 (MQ=255)

                                                                                                                              aCATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCg                     >  1:446237/1‑150 (MQ=255)

                                                                                                                                 tCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAgg                  >  1:1280561/1‑150 (MQ=255)

                                                                                                                                        ccatCATGCAGGCTTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAgttc           >  2:1611386/4‑150 (MQ=255)

                                                                                                                                        ccatCATGCAGGCTTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAgttc           >  1:1270897/4‑150 (MQ=255)

                                                                                                                                        ccatCATGCAGGCTTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAgttc           >  2:77928/4‑150 (MQ=255)

                                                                                                                                        ccatCATGCAGGCTTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAgttc           >  1:1174122/4‑150 (MQ=255)

                                                                                                                                         ggTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAgttcg          >  2:659160/1‑150 (MQ=255)

                                                                                                                                          atCATGCAGGCTTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAgttcgt         >  2:1605648/2‑150 (MQ=255)

                                                                                                                                             aTGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGttcgttcg      >  2:681320/1‑150 (MQ=255)

                                                                                                                                             aTGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGttcgttcg      <  1:668569/150‑1 (MQ=255)

                                                                                                                                              tGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCAAATTTTTCGCTTTCGCCAACTGCGCCAGTATGCTCAAGCAAAGCACCGAGGGAAGTTCGTGCGa     >  1:149920/1‑150 (MQ=255)

                                                                                                                                                cAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATg   >  1:1779705/1‑150 (MQ=255)

                                                                                                                                                cAAGCCTGCATGATGGTGCGGTAAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACAAAATACGCGTGCGGCATATATTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGGTCGATg   >  1:222481/1‑150 (MQ=255)

                                                                                                                                                 aaGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGc  <  2:1042740/150‑1 (MQ=255)

TCCTGCGGATAGATTGGCCCGATAATGCCGTAGCGGTTGCGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGC  >  NC_000913/4339656‑4339950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`