Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,353,171 C→G 7.9% intergenic (+90/+29) ghoT → / ← lysU toxin of GhoTS toxin‑antitoxin pair; membrane‑lytic protein; stimulator of persister cell formation/lysine tRNA synthetase, inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,353,1710CG 7.9% 442.5 / 22.6 146intergenic (+90/+29)ghoT/lysUtoxin of GhoTS toxin‑antitoxin pair; membrane‑lytic protein; stimulator of persister cell formation/lysine tRNA synthetase, inducible
Reads supporting (aligned to +/- strand):  ref base C (71/60);  new base G (9/3);  total (81/65)
Fisher's exact test for biased strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

Only 100 of 148 total aligned reads displayed.

CTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCAT  >  NC_000913/4353022‑4353318
                                                                                                                                                     |                                                                                                                                                   
   gTCGGAATAACCCGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTg                                                                                                                                                  >  1:1118358/1‑150 (MQ=255)
     cGGAATAACCTGGCCAATGAGGCTGCCGGGGGCATTACTTTTTTCTGTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGGCGCCTGAc                                                                                                                                                <  1:659165/150‑1 (MQ=255)
      ggAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACt                                                                                                                                               <  1:652485/150‑1 (MQ=255)
           aaCCTGGCCAATGAGTCTGCCTGTGGCTTTTCTTTTTTCTCTCTTATAGGAGCCGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGTAACGATGAACGAAGCAGTCAGGCGACTGCTTc                                                                                                                                          <  2:913924/150‑1 (MQ=255)
           aaCCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCAGGCGACTGCTTc                                                                                                                                          <  2:1157793/150‑1 (MQ=255)
            aCCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCg                                                                                                                                         <  1:1405396/150‑1 (MQ=255)
             ccTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGt                                                                                                                                        >  1:1285198/1‑150 (MQ=255)
             ccTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGt                                                                                                                                        <  1:456588/150‑1 (MQ=255)
             ccTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGGCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGt                                                                                                                                        <  1:471350/150‑1 (MQ=255)
             ccTGGCCAATGAGTCGGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCAGACTGCTTCGt                                                                                                                                        <  1:1244109/150‑1 (MQ=255)
              cTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTCGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGtt                                                                                                                                       <  1:365404/150‑1 (MQ=255)
              cTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGtt                                                                                                                                       >  2:1851242/1‑150 (MQ=255)
              cTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGtt                                                                                                                                       <  1:434187/150‑1 (MQ=255)
                  ccAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCAtt                                                                                                                                   >  1:1276200/1‑150 (MQ=255)
                  ccAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCAtt                                                                                                                                   <  1:956219/150‑1 (MQ=255)
                   cAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTa                                                                                                                                  <  1:1271051/150‑1 (MQ=255)
                         gTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGa                                                                                                                            >  2:430087/1‑150 (MQ=255)
                            tGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAat                                                                                                                         <  2:836969/150‑1 (MQ=255)
                            tGCCTGTGGCATTACTTTTTTCGCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAat                                                                                                                         >  2:71696/1‑150 (MQ=255)
                             gCCTGTGGCATTACTTTTTTCTCTCATTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGAGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAatt                                                                                                                        >  2:1642453/1‑150 (MQ=255)
                                 gtgGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAatttatt                                                                                                                    >  2:1320249/1‑150 (MQ=255)
                                   ggCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTc                                                                                                                  >  1:1145168/1‑150 (MQ=255)
                                    gCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCt                                                                                                                 >  2:1843651/1‑150 (MQ=255)
                                       ttACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCtgtg                                                                                                              <  1:545683/150‑1 (MQ=255)
                                       ttACTTTTTTCTCTCATTTAGGAGCGGAAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCtgtg                                                                                                              <  2:1674957/150‑1 (MQ=255)
                                         aCTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTggg                                                                                                            <  1:146742/150‑1 (MQ=255)
                                           ttttttCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGgcg                                                                                                          >  2:1034581/1‑150 (MQ=255)
                                             ttttCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCa                                                                                                        <  1:372808/150‑1 (MQ=255)
                                                tctctcTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCg                                                                                                     <  1:1429823/150‑1 (MQ=255)
                                                  tctctTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGcc                                                                                                   >  2:245865/1‑150 (MQ=255)
                                                  tctctTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGcc                                                                                                   >  1:612092/1‑150 (MQ=255)
                                                    tctTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCgg                                                                                                 <  1:389353/150‑1 (MQ=255)
                                                     ctTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGGCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCggg                                                                                                <  1:1791971/150‑1 (MQ=255)
                                                      ttttAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGa                                                                                               <  2:1748573/150‑1 (MQ=255)
                                                       tttAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGaa                                                                                              <  2:1377498/150‑1 (MQ=255)
                                                             aGCTGTAGTTGAGTGGACCCTCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCAGGGAAGAGAAt                                                                                        >  1:7575/1‑150 (MQ=255)
                                                             aGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGCAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCACTCGCCTGACTGCTACGTTCATTAAAGTGAAATTAATTTCTGTGGGCGCATCGCCGGGCAGAGAAt                                                                                        >  1:103512/1‑150 (MQ=255)
                                                             aGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCAATAAAGTGAAAATTATATCTGTGGGCGCATCGCCGGGAAGAGAAt                                                                                        >  1:273143/1‑150 (MQ=255)
                                                              gCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATa                                                                                       <  2:1285198/150‑1 (MQ=255)
                                                              gCTGTAGGTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATa                                                                                       <  2:498128/150‑1 (MQ=255)
                                                               cTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATaa                                                                                      >  1:709298/1‑150 (MQ=255)
                                                                tGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGTAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAAc                                                                                     <  1:1517553/150‑1 (MQ=255)
                                                                   aGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGAGAAATTTATTTCTGTGGGCGCATCGCCGCGAAGAGAATAACGTc                                                                                  >  1:1182600/1‑150 (MQ=255)
                                                                    gTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTcg                                                                                 <  1:706636/150‑1 (MQ=255)
                                                                      tGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTcgcg                                                                               >  2:409104/1‑150 (MQ=255)
                                                                      tGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTcgcg                                                                               >  2:1082418/1‑150 (MQ=255)
                                                                      tGAGTGGAACCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGAGTTGAGGAACGATTAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTcgcg                                                                               <  2:320633/150‑1 (MQ=255)
                                                                       gAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGa                                                                              >  2:1438769/1‑150 (MQ=255)
                                                                        aGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTGGCGGACCGATGAACGAAGCAGTCGCCTGAATGCTTCGTGCAGTAAAGTGAAATTTATGTCTGTGGGCGCATCGCCGGGAAGAGAATATCGTCGCGaa                                                                             >  1:86864/1‑150 (MQ=255)
                                                                                cGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATgg                                                                     >  1:1653755/1‑150 (MQ=255)
                                                                                   tGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTg                                                                  <  2:1621782/150‑1 (MQ=255)
                                                                                   tGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTg                                                                  <  2:804630/150‑1 (MQ=255)
                                                                                   tGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTCTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTg                                                                  >  1:880687/1‑150 (MQ=255)
                                                                                     gTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGtt                                                                <  2:1561879/150‑1 (MQ=255)
                                                                                     gTTATCGTGAAACGAAGCGGCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGtt                                                                <  2:1119075/150‑1 (MQ=255)
                                                                                           gTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGGCTGACGGCTTCGTTCATTTAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGGCGCGAATTGTATGGCTGTTAGTaaa                                                          >  2:940677/1‑150 (MQ=255)
                                                                                           gTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGATGAGAATAACGTCGCGAATAGTATGGCTGTTAggtaa                                                          >  2:158615/1‑146 (MQ=255)
                                                                                           gTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTaaa                                                          >  1:768509/1‑150 (MQ=255)
                                                                                           gTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGAGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCAGTAAAGTGAAATTTATTTCTGTGGGCGCATCTCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGGAAGTaaa                                                          >  1:447055/1‑150 (MQ=255)
                                                                                                 cGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCAt                                                    >  2:1083984/1‑150 (MQ=255)
                                                                                                      cGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCa                                               <  2:1766902/150‑1 (MQ=255)
                                                                                                         ctctcCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTc                                            <  1:850526/150‑1 (MQ=255)
                                                                                                         ctctcCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTc                                            <  2:186628/150‑1 (MQ=255)
                                                                                                          tctcCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCg                                           >  1:1449454/1‑150 (MQ=255)
                                                                                                           ctcctcAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTATACAGCATACTCATTCgt                                          >  1:306213/1‑149 (MQ=255)
                                                                                                           ctcctcAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCgg                                          <  2:838733/150‑1 (MQ=255)
                                                                                                           ctcctcAAAAATGGATTTTCTGAACGATTAACGAATCAGTCGACTGACTGATTAGTTCATTAAATTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCgg                                          <  2:1412220/150‑1 (MQ=255)
                                                                                                            tcctcAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGt                                         <  2:60843/150‑1 (MQ=255)
                                                                                                             cctcAACACTGGATTTGCGTAACGATGAACGACGCCGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGACTAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTc                                        <  1:323973/150‑1 (MQ=255)
                                                                                                             cctcAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTc                                        <  2:612092/150‑1 (MQ=255)
                                                                                                             cctcAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTACTGTGGGCGCATCGCCGGGAAGAGAAAAACGTCGCGAATAGTATGGCATTTAGTAAACAGCATAATCAttagtta                                        >  1:402047/1‑145 (MQ=255)
                                                                                                              ctcAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCg                                       >  1:1494425/1‑150 (MQ=255)
                                                                                                                cAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGAt                                     >  2:1034139/1‑150 (MQ=255)
                                                                                                                aaaaaCTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGAt                                     <  2:754417/149‑1 (MQ=255)
                                                                                                                   aaCTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc                                  <  1:1341708/150‑1 (MQ=255)
                                                                                                                            tGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAg                         >  2:506046/1‑150 (MQ=255)
                                                                                                                              cGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAATCAGCATAATCATTCGGTCGTTACCAATCCCCAGAc                       >  1:50563/1‑150 (MQ=255)
                                                                                                                              cGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAc                       >  1:1108454/1‑150 (MQ=255)
                                                                                                                              cGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAc                       <  2:1845423/150‑1 (MQ=255)
                                                                                                                              cGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAc                       >  1:1868360/1‑150 (MQ=255)
                                                                                                                               ggAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTGGCGAATTGTATTGCTGTTAGTAGACAGCATAATCATTCGGTCGATACCAATACCCAGAcc                      >  2:166604/1‑150 (MQ=255)
                                                                                                                                  aCGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGATTAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGc                   >  1:509833/1‑150 (MQ=255)
                                                                                                                                    gATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCgg                 <  1:1710216/150‑1 (MQ=255)
                                                                                                                                     aTGAACGAAGCAGTCAGGCGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGt                >  1:1265310/1‑150 (MQ=255)
                                                                                                                                       gAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTg              >  2:1247817/1‑150 (MQ=255)
                                                                                                                                       gAACGAAGCAGTCAGGCGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTg              >  1:1280576/1‑150 (MQ=255)
                                                                                                                                         aCGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTggc            >  2:1289591/1‑150 (MQ=255)
                                                                                                                                         aCGAAGCAGTCAGGCGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCACTACCCAGACCAGCGGTTggc            >  1:1263524/1‑150 (MQ=255)
                                                                                                                                         aCGAAGCAGTCAGGCGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTggc            >  2:784318/1‑150 (MQ=255)
                                                                                                                                         aCGAAGCAGTCAGGCGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGGATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTggc            >  1:963684/1‑150 (MQ=255)
                                                                                                                                          cGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATAGCCGGGAAGAGATTAACGTGGCGAATTGTATGGCTGTTAGTAAACAGGATAATCATTCGGTCGCTACCAATACCCAGACCAGCGGTTggcg           >  2:312822/1‑150 (MQ=255)
                                                                                                                                          cGAAGCAGTCAGGCGACTGCTTCGTTCATTAAAGTGAAATTTACTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTggcg           >  2:1258134/1‑150 (MQ=255)
                                                                                                                                           gAAGCAGTCAGGCGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTggcgg          >  2:1754644/1‑150 (MQ=255)
                                                                                                                                            aaGCAGTCTCCTCACTGCTTCGTTCATTAAAGTGAAATTTCTATCTGTGGGCGCATCGCCGGGAAGAGAATAACCTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTggcggc         <  2:1220574/150‑1 (MQ=255)
                                                                                                                                            aaGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTTTTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGTATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCAAGAACAGCGGTTggcggc         >  1:83441/1‑150 (MQ=255)
                                                                                                                                            aaGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTggcggc         <  1:900394/150‑1 (MQ=255)
                                                                                                                                            aaGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTggcggc         <  1:1593409/150‑1 (MQ=255)
                                                                                                                                             aGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCTCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCa        <  2:1264793/150‑1 (MQ=255)
                                                                                                                                                aGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCacac     <  2:292128/150‑1 (MQ=255)
                                                                                                                                                   cGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCat  <  1:1716511/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCAT  >  NC_000913/4353022‑4353318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: