Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 603,393 T→A 5.5% intergenic (+58/+23) pheP → / ← ybdG phenylalanine transporter/mechanosensitive channel protein, miniconductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913603,3930TA 5.5% 361.5 / 3.4 129intergenic (+58/+23)pheP/ybdGphenylalanine transporter/mechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand):  ref base T (60/61);  new base A (2/6);  total (62/67)
Fisher's exact test for biased strand distribution p-value = 2.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

CACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCAT  >  NC_000913/603245‑603540
                                                                                                                                                    |                                                                                                                                                   
cACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCAtt                                                                                                                                                    >  2:39863/1‑150 (MQ=255)
  agatgtatgATATGCGCTTGTCAGCGATCCTGCTGCGGGTGGGGATTGTAATCATGTTTATGGCATTTAAAACGCTGCGTCGGAAAAAAGGCATTCACGCTACAACCGAAAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCg                                                                                                                                                  <  2:674280/149‑1 (MQ=255)
   gatggatgATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTATCATCCATTCGa                                                                                                                                                 >  1:947890/1‑150 (MQ=255)
      ggatgATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATga                                                                                                                                              >  2:437171/1‑150 (MQ=255)
       gatgatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATgag                                                                                                                                             <  2:871398/150‑1 (MQ=255)
       gaggatATGCGCTTGTCATCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATgag                                                                                                                                             <  1:1082667/147‑1 (MQ=255)
        atgatATGCGCTTGTCAGCGAGCCTGCAGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATgaga                                                                                                                                            <  2:943515/150‑1 (MQ=255)
         tgatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGagag                                                                                                                                           <  2:1653947/150‑1 (MQ=255)
         tgaactGCGCGTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGagag                                                                                                                                           <  2:938971/145‑1 (MQ=255)
          gatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCGAATGGATGAGAGc                                                                                                                                          <  2:1769065/150‑1 (MQ=255)
          gatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGAGGAGAGc                                                                                                                                          >  1:325991/1‑150 (MQ=255)
           atatGCTCTTGTCCGCGATCCTGCTGCCGTTGTGGATTGTATTCCTGTTTATGGCATTTAAAACTCTGCGTCTGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGagagnn                                                                                                                                         <  2:50801/150‑3 (MQ=255)
           atatGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCGAATGGATGAGAGCg                                                                                                                                         <  1:1630899/150‑1 (MQ=255)
           atatGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCg                                                                                                                                         >  1:181709/1‑150 (MQ=255)
           atatGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCg                                                                                                                                         >  1:452504/1‑150 (MQ=255)
            tctGCGCTGGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGGCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCGAATGGATGAGAGCgg                                                                                                                                        <  2:149429/148‑1 (MQ=255)
               gcgcTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGttt                                                                                                                                     <  1:196555/150‑1 (MQ=255)
                 gcTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTTTTCGTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTTCATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGttttt                                                                                                                                   <  1:1396677/150‑1 (MQ=255)
                    tGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTaa                                                                                                                               <  1:1392076/150‑1 (MQ=255)
                     gTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTaa                                                                                                                               >  1:1512837/1‑150 (MQ=255)
                         gCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTAc                                                                                                                           <  2:1699316/150‑1 (MQ=255)
                               ctgctgCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTa                                                                                                                     >  1:670948/1‑150 (MQ=255)
                               ctgctgCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATACAATCGATGAGAGAGGTTTTTTTAATTACTGCTTa                                                                                                                     >  2:103915/1‑150 (MQ=255)
                                 gctgcCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTaaa                                                                                                                   <  2:1839245/150‑1 (MQ=255)
                                   tgcCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATg                                                                                                                 <  2:1821765/150‑1 (MQ=255)
                                      cGGTGTGGATTGTATTCCTGTTTATGGCATTAAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCAc                                                                                                              >  1:62370/1‑150 (MQ=255)
                                           tgGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGcc                                                                                                         <  2:455593/150‑1 (MQ=255)
                                            ggATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCa                                                                                                        >  1:1416666/1‑150 (MQ=255)
                                             gATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCag                                                                                                       >  2:136311/1‑150 (MQ=255)
                                              aTTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCaga                                                                                                      >  1:1571900/1‑150 (MQ=255)
                                              aTTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCaga                                                                                                      >  1:260666/1‑150 (MQ=255)
                                               ttGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGAATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCagag                                                                                                     <  1:1072128/150‑1 (MQ=255)
                                                tGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGTTGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCagaga                                                                                                    <  1:1226234/150‑1 (MQ=255)
                                                tGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCAATCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCagaga                                                                                                    <  1:36399/150‑1 (MQ=255)
                                                 gTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCagagag                                                                                                   <  1:540532/150‑1 (MQ=255)
                                                    ttCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGa                                                                                                >  1:19374/1‑150 (MQ=255)
                                                     tCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTAGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGAGaa                                                                                               >  2:258406/1‑150 (MQ=255)
                                                      ccTGTTTATGGCATTTAAACCGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGaaa                                                                                              >  1:283374/1‑149 (MQ=255)
                                                       cTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAata                                                                                             <  1:1343124/150‑1 (MQ=255)
                                                         gTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATc                                                                                           >  1:1103871/1‑150 (MQ=255)
                                                              tGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGc                                                                                      >  2:1794528/1‑150 (MQ=255)
                                                                 cATTTAACACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGAGGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCgg                                                                                   <  2:128824/150‑1 (MQ=255)
                                                                 cATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCgg                                                                                   <  1:723740/150‑1 (MQ=255)
                                                                    ttAAAACGCTGCGTCGGAAATAAGGCATTCAAGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTg                                                                                <  2:698958/150‑1 (MQ=255)
                                                                     tAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTgg                                                                               <  2:1340145/150‑1 (MQ=255)
                                                                     tAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTgg                                                                               >  1:1050180/1‑150 (MQ=255)
                                                                     tAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTgg                                                                               <  2:610926/150‑1 (MQ=255)
                                                                      aaaaCGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGc                                                                              <  2:654196/150‑1 (MQ=255)
                                                                       aaaaGCTGCGTCTTAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGATACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCg                                                                             <  2:1480483/150‑1 (MQ=255)
                                                                       aaaCGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCg                                                                             >  1:1056072/1‑150 (MQ=255)
                                                                       aaaCGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCg                                                                             >  1:1617955/1‑150 (MQ=255)
                                                                            cTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGAGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACtga                                                                        >  2:525106/1‑150 (MQ=255)
                                                                             ggCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCCTCCGAACCCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACtgat                                                                       <  1:922404/149‑1 (MQ=255)
                                                                              gCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACtgatg                                                                      <  1:338055/150‑1 (MQ=255)
                                                                              gCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACtgatg                                                                      <  1:848985/150‑1 (MQ=255)
                                                                                 tCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAg                                                                   <  1:1140046/150‑1 (MQ=255)
                                                                                      aaTAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCa                                                              <  2:928836/150‑1 (MQ=255)
                                                                                      aaTAAGGCATTCACGATACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCa                                                              <  2:1259256/150‑1 (MQ=255)
                                                                                       aTAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAg                                                             <  2:829402/150‑1 (MQ=255)
                                                                                        tAAGGCATTCACGCTACCTCCGACAAAACGATGGCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGa                                                            <  1:1678081/150‑1 (MQ=255)
                                                                                        tAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAgc                                                            >  1:960406/1‑149 (MQ=255)
                                                                                        tAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGa                                                            >  2:1464556/1‑150 (MQ=255)
                                                                                        tAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGa                                                            >  2:1856798/1‑150 (MQ=255)
                                                                                         acggCAATCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAACGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGAc                                                           <  1:1237409/148‑1 (MQ=255)
                                                                                         aaGGCATTCATGCTACATCCGACAAAACGATGGCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGAc                                                           <  1:1472358/150‑1 (MQ=255)
                                                                                             cATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCaaa                                                       >  2:1048139/1‑150 (MQ=255)
                                                                                              aTTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAAc                                                      <  1:1113232/150‑1 (MQ=255)
                                                                                                    gCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCg                                                <  2:1261854/150‑1 (MQ=255)
                                                                                                      tACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGAc                                              <  1:657330/150‑1 (MQ=255)
                                                                                                        cATCCGACAACACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCAACCGCCAGAGAGCGAATATATTTGCCGGTTGGCGACTGATGAAGACGCAGACAAATCTCTTAGACaa                                            >  2:289783/1‑150 (MQ=255)
                                                                                                        cATCCGACAAAACGATGTCAACCCTCCGAAACCGCTCGCATCCATTCGATGAGAGCGGTTTTTTTAAATACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACaa                                            <  2:979056/150‑1 (MQ=255)
                                                                                                         aTCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAAt                                           >  2:701138/1‑150 (MQ=255)
                                                                                                           ccGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCg                                         <  2:1869901/150‑1 (MQ=255)
                                                                                                            cGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATTCACCCGCCAGAGAGCGAATATCATTGCCGGTTGTCGACTGATGAACTCGCAGACCAAACTCTGCGACAATCGc                                        >  2:263167/1‑150 (MQ=255)
                                                                                                            cGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGc                                        >  1:418540/1‑150 (MQ=255)
                                                                                                                  aaCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAatat                                  <  2:1571900/150‑1 (MQ=255)
                                                                                                                      aTGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGa                              >  2:640599/1‑150 (MQ=255)
                                                                                                                       tGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGAt                             <  2:1669793/150‑1 (MQ=255)
                                                                                                                           aaCCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGaa                         <  2:1749096/150‑1 (MQ=255)
                                                                                                                            aCCATCCGAAACCGCTCTCATACATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCAGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAat                        <  1:1291423/150‑1 (MQ=255)
                                                                                                                             ccATCCGAAACCGCTCTCATCCATTCGATGAGAGCTGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAata                       <  1:1568649/150‑1 (MQ=255)
                                                                                                                              cATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAatat                      >  1:1485621/1‑150 (MQ=255)
                                                                                                                              cATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAatat                      >  2:474567/1‑150 (MQ=255)
                                                                                                                              cATCCGAAACCGCTCTCATCCAATCGATGAGAGCGGTTTTTTTAATTCCTGCTTAAATGCACCCGCCAGAGAGCGAAGATCATTGCCGGTTGGCGACTGATGAACTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAatat                      <  1:275316/150‑1 (MQ=255)
                                                                                                                                   gAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAg                 <  1:908101/150‑1 (MQ=255)
                                                                                                                                     aaCCGCTCTCATCGAATGGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGcc               >  2:1157167/1‑150 (MQ=255)
                                                                                                                                     aaCCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGcc               <  1:1778011/150‑1 (MQ=255)
                                                                                                                                     aaCCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGcc               <  1:1794528/150‑1 (MQ=255)
                                                                                                                                       cctctctCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCATAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGGAAATATGTGATCGAATATATCAGCCTg             <  2:1225943/147‑1 (MQ=255)
                                                                                                                                       ccGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTg             <  2:1056072/150‑1 (MQ=255)
                                                                                                                                        cGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGa            >  1:1571177/1‑150 (MQ=255)
                                                                                                                                         gCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGAAGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATAAGCCTGaa           >  1:1782341/1‑150 (MQ=255)
                                                                                                                                            ctcATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGc        >  1:74485/1‑150 (MQ=255)
                                                                                                                                              cATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCGGCCTGAATGcgt      >  2:1858479/1‑148 (MQ=255)
                                                                                                                                               aTCCATTCGATGAGAGCGGTTTTTTTAATTTCTGGTTAAATGCACCCGCCAGAGTGCGAATATCATTGCCGGTTGGCGACTGATGTAGTCGCAGACCATACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCttt     <  1:941565/150‑1 (MQ=255)
                                                                                                                                                 ccATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCa   <  2:1512837/150‑1 (MQ=255)
                                                                                                                                                 ccATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCa   >  1:566127/1‑150 (MQ=255)
                                                                                                                                                 ccATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCa   >  2:1849513/1‑150 (MQ=255)
                                                                                                                                                  cATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGGTGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCat  >  2:916528/1‑150 (MQ=255)
                                                                                                                                                  cATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCat  >  2:426119/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCAT  >  NC_000913/603245‑603540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: