Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,415,526 A→C 11.8% M418L (ATG→CTG) ‡ aidB → DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,415,5260AC11.8% 327.1 / 29.7 131M418L (ATG→CTG) ‡aidBDNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (56/59);  new base C (8/8);  total (64/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.24e-01

Only 100 of 136 total aligned reads displayed.

CCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGT  >  NC_000913/4415377‑4415674
                                                                                                                                                     |                                                                                                                                                    
ccAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTATGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGgaga                                                                                                                                                      >  2:1062154/1‑150 (MQ=255)
 cAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGAt                                                                                                                                                     >  1:1018328/1‑150 (MQ=255)
     gaagCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCg                                                                                                                                                 >  1:573481/1‑150 (MQ=255)
      aagCCCTGTGGGCGCGTTTATTTACGCCGGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCgg                                                                                                                                                <  1:857017/150‑1 (MQ=255)
       atcccTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGGGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGt                                                                                                                                               <  1:925215/148‑1 (MQ=255)
        gCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTa                                                                                                                                              <  2:1758996/150‑1 (MQ=255)
            tgtgGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGCATCTCCCGGTaaagc                                                                                                                                          <  1:1642673/150‑3 (MQ=255)
            tgtgGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGGTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGCATCTCCCGGTaaagc                                                                                                                                          <  1:667606/150‑3 (MQ=255)
            tgtgGGCGCGTCTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGGGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGCATCTCCCGGTaaagc                                                                                                                                          <  1:450358/150‑3 (MQ=255)
             gtgGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAg                                                                                                                                         <  1:1024666/150‑1 (MQ=255)
               gggCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCTGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTa                                                                                                                                       >  2:956551/1‑150 (MQ=255)
               gggCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTTCCGGGAGATGCCGGTAAACAGTa                                                                                                                                       >  2:341192/1‑150 (MQ=255)
               gggCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCAGTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTa                                                                                                                                       >  2:1463685/1‑150 (MQ=255)
                  cgcgTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTAttt                                                                                                                                    <  1:382672/150‑1 (MQ=255)
                   gcgTTAATTTCCGCCTGCGGCAAAATTTGTGATCAGCAAAAGAGGGATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGACGGTAAACAGTATTTg                                                                                                                                   <  2:459830/150‑1 (MQ=255)
                      tttatttaCGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGCAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGa                                                                                                                                >  1:1668279/1‑150 (MQ=255)
                      tttatttaCGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGa                                                                                                                                >  2:49263/1‑150 (MQ=255)
                      attatttaCGCCTGCGGCAAAATTTGTGATCTGCAATAGACATATGCACTTTGAGGACCAAGCGATGGAGGTGCTGGGCGGCATTCGTTTTTGCGAGGAGAGACAGCTGCCGCGACTTTACCGGGAGATGCCGGTAAACAGTATTTgtca                                                                                                                                >  1:320967/2‑147 (MQ=255)
                         atttaCGCCTGCGGCAAAATTTCTGATCTCCAAAAGAGGTATGCCGCTTGTGGCCGAAGCGAGGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAgg                                                                                                                             <  2:252138/150‑1 (MQ=255)
                              cGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAACCAGGATTTGGGATGGTTCCg                                                                                                                        >  2:86968/1‑150 (MQ=255)
                               gCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCgg                                                                                                                       <  2:1485376/150‑1 (MQ=255)
                               cacTGCGGAAAAATTTTGTATCTGCAAAAGAGGTATGACGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGTGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGTGAAGGTTCCgg                                                                                                                       <  2:655053/148‑1 (MQ=255)
                                   gCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAat                                                                                                                   <  1:1286694/150‑1 (MQ=255)
                                   gCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAat                                                                                                                   <  2:1120096/150‑1 (MQ=255)
                                    cGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAata                                                                                                                  >  2:389269/1‑150 (MQ=255)
                                       cAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGAAGGAGGAGCTGGGCGCCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCTGTAAACAGTATTTGGGAAGGTTCCGGCAAtatta                                                                                                               >  1:1213999/1‑150 (MQ=255)
                                          aaTTTTTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTAtgt                                                                                                            <  1:1277026/150‑1 (MQ=255)
                                          aaTTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTAtgt                                                                                                            >  2:1565215/1‑150 (MQ=255)
                                          aaTTTGTGATATGCAAAAGAGGTATGCCGTTTGTGGCCGTAGCGTTGGAGGTGCTGTGCTGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGTCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTAtgt                                                                                                            <  1:322077/150‑1 (MQ=255)
                                          aaTTTGGGATCTGCAAATGAGGTATGCCGGTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTAtgt                                                                                                            <  1:629458/150‑1 (MQ=255)
                                            tttGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGc                                                                                                          <  2:1455280/150‑1 (MQ=255)
                                               gtgATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTg                                                                                                       >  1:398977/1‑150 (MQ=255)
                                                 gATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGTGAGTGAGCTGCAGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGAAATATTATGTGCCTGGa                                                                                                     >  1:81442/1‑150 (MQ=255)
                                                  aTCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTACACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGAt                                                                                                    >  1:1429933/1‑150 (MQ=255)
                                                     tGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAATCAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGAtgtg                                                                                                 >  2:186066/1‑150 (MQ=255)
                                                        aaaaGAGGTATGCCGTTTGTGGCCGAAGCCATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACAGGGAGATGCCGGTAAACAGTATTTGGGATGGTTCCGGCAATATTATGTGCCTGGATGTGTTg                                                                                              >  1:150803/1‑150 (MQ=255)
                                                         aaaGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCTAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTgc                                                                                             >  2:96208/1‑150 (MQ=255)
                                                          aaGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTgcg                                                                                            <  1:389269/150‑1 (MQ=255)
                                                            tagGTATGCCGTTGGTGGCCGAAGCGATGGATGTGCTGGGCGTGAATGGTTATTGCGATGAGAGTCAGCTGCCGCGTCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGcgcg                                                                                          <  1:330443/149‑1 (MQ=255)
                                                            gagGTATGCCGTTTGAGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGGGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTAATTGGGAAGGTTCCGGCAAAATTATGTGCCTGGATGTGTTGcgcg                                                                                          <  2:1643992/150‑1 (MQ=255)
                                                               gTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTtc                                                                                       <  2:973888/150‑1 (MQ=255)
                                                               gTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTtc                                                                                       <  2:653096/150‑1 (MQ=255)
                                                                tATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTtct                                                                                      <  1:1386253/150‑1 (MQ=255)
                                                                tATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTtct                                                                                      >  1:1186/1‑150 (MQ=255)
                                                                 ctGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTtctc                                                                                     <  2:1838279/149‑1 (MQ=255)
                                                                  tgtcGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCa                                                                                    <  1:14252/147‑1 (MQ=255)
                                                                  tGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTATACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCa                                                                                    <  1:1045643/150‑1 (MQ=255)
                                                                  tGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCa                                                                                    >  1:825660/1‑150 (MQ=255)
                                                                   gCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCaa                                                                                   <  2:627538/150‑1 (MQ=255)
                                                                    ccGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAAt                                                                                  >  1:1516711/1‑150 (MQ=255)
                                                                        ttGTGGCCGCTGCGATCGAGGTGCTGGGCGGGATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCAGGTAAACATTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaagc                                                                              <  2:1251760/150‑1 (MQ=255)
                                                                         tgtgGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaagca                                                                             >  2:1722523/1‑150 (MQ=255)
                                                                         tgtgGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGAGAGCGGCCGCGGCGTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaagca                                                                             <  1:794501/150‑1 (MQ=255)
                                                                            ggCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaagcaagc                                                                          >  2:1646332/1‑150 (MQ=255)
                                                                              ccGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTTCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCgg                                                                        >  1:1108063/1‑150 (MQ=255)
                                                                              ccGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATACGCAAGCgg                                                                        >  1:344564/1‑150 (MQ=255)
                                                                              ccGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCgg                                                                        >  1:601879/1‑150 (MQ=255)
                                                                              ccGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCgg                                                                        >  1:1269667/1‑150 (MQ=255)
                                                                                gAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGGGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGc                                                                      >  2:666371/1‑150 (MQ=255)
                                                                                      aTGGAGGTGCTGGGCGGCATTGGGTATTGCGAGGAGAGGGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGGTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATAc                                                                <  2:755070/150‑1 (MQ=255)
                                                                                       tGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACg                                                               <  1:1641504/150‑1 (MQ=255)
                                                                                        ggAGGTGCAGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGa                                                              >  2:1853149/1‑150 (MQ=255)
                                                                                           ggTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACtt                                                           <  2:932799/150‑1 (MQ=255)
                                                                                            gTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTa                                                          >  2:5731/1‑150 (MQ=255)
                                                                                             tGCTGGGCGGCATTGGTTATTGCGAGGAGTGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTAt                                                         <  2:1513151/150‑1 (MQ=255)
                                                                                              gCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTAtt                                                        >  2:1074577/1‑150 (MQ=255)
                                                                                                  ggcggcATTGGATATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGCTGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCg                                                    <  2:148519/150‑1 (MQ=255)
                                                                                                     ggcATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGaa                                                 <  1:1241358/150‑1 (MQ=255)
                                                                                                              tATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTtgtg                                        <  1:1070799/150‑1 (MQ=255)
                                                                                                               aTTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTgg                                       >  2:1148998/1‑150 (MQ=255)
                                                                                                                  gCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAg                                    >  1:1773168/1‑150 (MQ=255)
                                                                                                                  gCGAGCAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAg                                    <  1:540521/150‑1 (MQ=255)
                                                                                                                    gaggagAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTAGGGAAGGTTCCGGCAATATTATGTGCGTGGATGTGTTGCGCGTTCTCAATCAGCAAGCGGGCGTATACGCCTTATTGTCGGAAGCATTTGTGGAAGTg                                  >  2:1131425/1‑150 (MQ=255)
                                                                                                                     aggagAGTGAGCTGCCGCGGCTTTACCGGGGGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGa                                 >  1:737037/1‑150 (MQ=255)
                                                                                                                        agagTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAg                              <  2:573481/150‑1 (MQ=255)
                                                                                                                              aGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAgg                        >  1:904750/1‑150 (MQ=255)
                                                                                                                                  gCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCg                    >  2:1363515/1‑150 (MQ=255)
                                                                                                                                  gCCGCGGCGTTACCGGGAGCTGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCg                    <  2:64514/150‑1 (MQ=255)
                                                                                                                                   ccGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGGGAAAGGGCAGGCTCGc                   <  2:1269667/150‑1 (MQ=255)
                                                                                                                                     gcgGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTa                 >  1:1464969/1‑150 (MQ=255)
                                                                                                                                       ggCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtt               <  2:398977/150‑1 (MQ=255)
                                                                                                                                       ctgtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGATGTGAAAGGGCAGGATCGCTAtt               >  1:1091218/4‑150 (MQ=255)
                                                                                                                                       ctgtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtt               >  2:1340802/4‑150 (MQ=255)
                                                                                                                                        tgtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAttt              >  2:1048422/3‑150 (MQ=255)
                                                                                                                                        gCTTTAGCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAttt              <  1:440234/150‑1 (MQ=255)
                                                                                                                                         gtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATTTTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTTTTGTCGGAAGCATTTGTGGAAGTGAAAGGGCTGGATCGCTAtttt             >  1:371962/2‑150 (MQ=255)
                                                                                                                                         gtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtttt             >  1:87322/2‑150 (MQ=255)
                                                                                                                                         gtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtttt             >  1:1734901/2‑150 (MQ=255)
                                                                                                                                         gtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGGGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtttt             >  1:1323252/2‑150 (MQ=255)
                                                                                                                                         cTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGAAATATTATGTGCCTGGATGTGATGCGCGTGCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtttt             <  1:86968/150‑1 (MQ=255)
                                                                                                                                           ttACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGACTGGATGTCTTGCGCGTTCTCAATAAGCAATCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGAGCGCTATTTTGa           >  2:121637/1‑150 (MQ=255)
                                                                                                                                             agccggAGCTGCCCGTAAACAGGATGTGGGAAGCCGCCGGCAATATTAAGTCCCGGGATGTGAAGCGCGTTCTCAATAAGCAATCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGGGAAAGGGCAGGATCGCTATTATGATc         <  2:354320/146‑1 (MQ=255)
                                                                                                                                             aCCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCTATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCTAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATc         <  2:1353400/150‑1 (MQ=255)
                                                                                                                                             aCCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATc         <  1:1565215/150‑1 (MQ=255)
                                                                                                                                             aCCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATc         <  2:408918/150‑1 (MQ=255)
                                                                                                                                              ccGGGAGATGCCGGTAAACAGTAATTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATcg        >  2:899406/1‑150 (MQ=255)
                                                                                                                                                 ggAGATGCCTGTAAACAGTAATTGGGAAGGTTCCGGCAATAATATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATcgcgc     <  2:746294/150‑1 (MQ=255)
                                                                                                                                                  gagaTGCCGGTAAACAGTATATGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATcgcgcg    <  2:605968/150‑1 (MQ=255)
                                                                                                                                                    gaTGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCTTACTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGt  >  1:300945/1‑150 (MQ=255)
                                                                                                                                                    gaTGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGt  >  2:391982/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGT  >  NC_000913/4415377‑4415674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: