Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,415,528 G→C 9.0% M418I (ATG→ATC) ‡ aidB → DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,415,5280GC 9.0% 404.7 / 28.5 134M418I (ATG→ATC) ‡aidBDNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (57/65);  new base C (8/4);  total (65/69)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.86e-01

Only 100 of 135 total aligned reads displayed.

AAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCG  >  NC_000913/4415380‑4415677
                                                                                                                                                    |                                                                                                                                                     
aagaagCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGc                                                                                                                                                      >  1:746294/1‑150 (MQ=255)
aagaagCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGc                                                                                                                                                      >  1:168102/1‑150 (MQ=255)
  gaagCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCg                                                                                                                                                    >  1:573481/1‑150 (MQ=255)
   aagCCCTGTGGGCGCGTTTATTTACGCCGGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCgg                                                                                                                                                   <  1:857017/150‑1 (MQ=255)
    atcccTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGGGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGt                                                                                                                                                  <  1:925215/148‑1 (MQ=255)
     gCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTa                                                                                                                                                 <  2:1758996/150‑1 (MQ=255)
        cTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGCATCTCCCGGTaaag                                                                                                                                              <  1:1414547/150‑2 (MQ=255)
         tgtgGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGCATCTCCCGGTaaagc                                                                                                                                             <  1:1642673/150‑3 (MQ=255)
          gtgGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAg                                                                                                                                            <  1:1024666/150‑1 (MQ=255)
            gggCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCTGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTa                                                                                                                                          >  2:956551/1‑150 (MQ=255)
            gggCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTTCCGGGAGATGCCGGTAAACAGTa                                                                                                                                          >  2:341192/1‑150 (MQ=255)
               cgcgTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTAttt                                                                                                                                       <  1:382672/150‑1 (MQ=255)
                gcgTTAATTTCCGCCTGCGGCAAAATTTGTGATCAGCAAAAGAGGGATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGACGGTAAACAGTATTTg                                                                                                                                      <  2:459830/150‑1 (MQ=255)
                 cgTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGCATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTgg                                                                                                                                     <  1:1251831/150‑1 (MQ=255)
                   tttatttaCGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGCAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGa                                                                                                                                   >  1:1668279/1‑150 (MQ=255)
                      atttaCGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAgg                                                                                                                                <  1:1875660/150‑1 (MQ=255)
                      atttaCGCCTGCGGCAAAATTTCTGATCTCCAAAAGAGGTATGCCGCTTGTGGCCGAAGCGAGGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAgg                                                                                                                                <  2:252138/150‑1 (MQ=255)
                           cGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAACCAGGATTTGGGATGGTTCCg                                                                                                                           >  2:86968/1‑150 (MQ=255)
                            gCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCgg                                                                                                                          <  2:1485376/150‑1 (MQ=255)
                            cacTGCGGAAAAATTTTGTATCTGCAAAAGAGGTATGACGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGTGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGTGAAGGTTCCgg                                                                                                                          <  2:655053/148‑1 (MQ=255)
                             ccTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGTCATTTGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGc                                                                                                                         >  1:379452/1‑150 (MQ=255)
                                gCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAat                                                                                                                      <  2:1120096/150‑1 (MQ=255)
                                gCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAat                                                                                                                      <  1:1286694/150‑1 (MQ=255)
                                 cGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAata                                                                                                                     >  2:389269/1‑150 (MQ=255)
                                    cAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGAAGGAGGAGCTGGGCGCCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCTGTAAACAGTATTTGGGAAGGTTCCGGCAAtatta                                                                                                                  >  1:1213999/1‑150 (MQ=255)
                                       aaTTTTTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTAtgt                                                                                                               <  1:1277026/150‑1 (MQ=255)
                                       aaTTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTAtgt                                                                                                               >  2:1565215/1‑150 (MQ=255)
                                       aaTTTGTGATATGCAAAAGAGGTATGCCGTTTGTGGCCGTAGCGTTGGAGGTGCTGTGCTGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGTCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTAtgt                                                                                                               <  1:322077/150‑1 (MQ=255)
                                       aaTTTGGGATCTGCAAATGAGGTATGCCGGTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTAtgt                                                                                                               <  1:629458/150‑1 (MQ=255)
                                         tttGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGc                                                                                                             <  2:1455280/150‑1 (MQ=255)
                                            gtgATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTg                                                                                                          >  1:398977/1‑150 (MQ=255)
                                              gATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGa                                                                                                        >  2:1852605/1‑150 (MQ=255)
                                                tCTGCAAAAGAGGGATGCCGTTTGTGGCCGAAGCGATGGCGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGAtg                                                                                                      <  2:1286897/150‑1 (MQ=255)
                                                  tGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAATCAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGAtgtg                                                                                                    >  2:186066/1‑150 (MQ=255)
                                                  tGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGAtgtg                                                                                                    >  1:1593015/1‑150 (MQ=255)
                                                  tGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGAtgtg                                                                                                    >  1:844370/1‑150 (MQ=255)
                                                     aaaaGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTg                                                                                                 <  2:1093888/150‑1 (MQ=255)
                                                     aaaaGAGGTATGCCGTTTGTGGCCGAAGCCATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACAGGGAGATGCCGGTAAACAGTATTTGGGATGGTTCCGGCAATATTATGTGCCTGGATGTGTTg                                                                                                 >  1:150803/1‑150 (MQ=255)
                                                      aaaGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCTAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTgc                                                                                                >  2:96208/1‑150 (MQ=255)
                                                         tagGTATGCCGTTGGTGGCCGAAGCGATGGATGTGCTGGGCGTGAATGGTTATTGCGATGAGAGTCAGCTGCCGCGTCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGcgcg                                                                                             <  1:330443/149‑1 (MQ=255)
                                                         gagGTATGCCGTTTGAGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGGGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTAATTGGGAAGGTTCCGGCAAAATTATGTGCCTGGATGTGTTGcgcg                                                                                             <  2:1643992/150‑1 (MQ=255)
                                                            gTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTtc                                                                                          <  2:653096/150‑1 (MQ=255)
                                                            gTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTtc                                                                                          <  2:973888/150‑1 (MQ=255)
                                                             tATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTtct                                                                                         <  1:1386253/150‑1 (MQ=255)
                                                               tgtcGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCa                                                                                       <  1:14252/147‑1 (MQ=255)
                                                               tGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTATACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCa                                                                                       <  1:1045643/150‑1 (MQ=255)
                                                               tGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCa                                                                                       >  1:825660/1‑150 (MQ=255)
                                                                gCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCaa                                                                                      <  2:627538/150‑1 (MQ=255)
                                                                 ccGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGCGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAAt                                                                                     >  2:1190293/1‑150 (MQ=255)
                                                                     ttGTGGCCGCTGCGATCGAGGTGCTGGGCGGGATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCAGGTAAACATTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaagc                                                                                 <  2:1251760/150‑1 (MQ=255)
                                                                      tgtgGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaagca                                                                                >  2:1722523/1‑150 (MQ=255)
                                                                      tgtgGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGAGAGCGGCCGCGGCGTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaagca                                                                                <  1:794501/150‑1 (MQ=255)
                                                                          gCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCg                                                                            <  1:1483098/150‑1 (MQ=255)
                                                                           ccGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTTCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCgg                                                                           >  1:1108063/1‑150 (MQ=255)
                                                                             gAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGGGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGc                                                                         >  2:666371/1‑150 (MQ=255)
                                                                             gAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAAGAAGCAAGCGGGc                                                                         >  1:1169340/1‑150 (MQ=255)
                                                                              aaGCGAGGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGGGCTCAATAAGCAAGCGGGCg                                                                        >  2:1479964/1‑150 (MQ=255)
                                                                                   aTGGAGGTGCTGGGCGGCATTGGGTATTGCGAGGAGAGGGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGGTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATAc                                                                   <  2:755070/150‑1 (MQ=255)
                                                                                     ggAGGTGCAGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGa                                                                 >  2:1853149/1‑150 (MQ=255)
                                                                                        ggTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACtt                                                              <  2:932799/150‑1 (MQ=255)
                                                                                         gTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTa                                                             <  1:607299/150‑1 (MQ=255)
                                                                                         gTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTa                                                             >  2:5731/1‑150 (MQ=255)
                                                                                               ggcggcATTGGATATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGCTGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCg                                                       <  2:148519/150‑1 (MQ=255)
                                                                                                  ggcATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGaa                                                    <  1:1241358/150‑1 (MQ=255)
                                                                                                               gCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAg                                       >  1:1773168/1‑150 (MQ=255)
                                                                                                               gCGAGCAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAg                                       <  1:540521/150‑1 (MQ=255)
                                                                                                                 gaggagAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTAGGGAAGGTTCCGGCAATATTATGTGCGTGGATGTGTTGCGCGTTCTCAATCAGCAAGCGGGCGTATACGCCTTATTGTCGGAAGCATTTGTGGAAGTg                                     >  2:1131425/1‑150 (MQ=255)
                                                                                                                  aggagAGTGAGCTGCCGCGGCTTTACCGGGGGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGa                                    >  1:737037/1‑150 (MQ=255)
                                                                                                                     agagTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAg                                 <  2:573481/150‑1 (MQ=255)
                                                                                                                       agTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAggg                               >  1:546762/1‑150 (MQ=255)
                                                                                                                        gTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaagca                                                                                >  1:405068/1‑100 (MQ=255)
                                                                                                                        gTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaagca                                                                                <  2:405068/100‑1 (MQ=255)
                                                                                                                           aGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAgg                           >  1:889163/1‑150 (MQ=255)
                                                                                                                              tGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATc                        <  2:1380028/150‑1 (MQ=255)
                                                                                                                               gCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCg                       >  2:1363515/1‑150 (MQ=255)
                                                                                                                               gCCGCGGCGTTACCGGGAGCTGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCg                       <  2:64514/150‑1 (MQ=255)
                                                                                                                                  gcgGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTa                    >  1:1464969/1‑150 (MQ=255)
                                                                                                                                    ctgtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGATGTGAAAGGGCAGGATCGCTAtt                  >  1:1091218/4‑150 (MQ=255)
                                                                                                                                    ctgtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtt                  >  2:1340802/4‑150 (MQ=255)
                                                                                                                                    ctgtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtt                  >  2:1537082/4‑150 (MQ=255)
                                                                                                                                     tgtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAttt                 >  2:1048422/3‑150 (MQ=255)
                                                                                                                                     gcgttACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAttt                 <  2:60844/147‑1 (MQ=255)
                                                                                                                                     gCTTTAGCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAttt                 <  1:440234/150‑1 (MQ=255)
                                                                                                                                      gtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATTTTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTTTTGTCGGAAGCATTTGTGGAAGTGAAAGGGCTGGATCGCTAtttt                >  1:371962/2‑150 (MQ=255)
                                                                                                                                      gtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtttt                >  1:87322/2‑150 (MQ=255)
                                                                                                                                      cTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtttt                <  2:1018328/150‑1 (MQ=255)
                                                                                                                                       tttACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTg               <  1:327519/150‑1 (MQ=255)
                                                                                                                                        ttACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGa              >  1:1052985/1‑150 (MQ=255)
                                                                                                                                        ttACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGACTGGATGTCTTGCGCGTTCTCAATAAGCAATCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGAGCGCTATTTTGa              >  2:121637/1‑150 (MQ=255)
                                                                                                                                          agccggAGCTGCCCGTAAACAGGATGTGGGAAGCCGCCGGCAATATTAAGTCCCGGGATGTGAAGCGCGTTCTCAATAAGCAATCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGGGAAAGGGCAGGATCGCTATTATGATc            <  2:354320/146‑1 (MQ=255)
                                                                                                                                          aCCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATc            <  2:408918/150‑1 (MQ=255)
                                                                                                                                          aCCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATc            <  1:1565215/150‑1 (MQ=255)
                                                                                                                                           ccGGGAGATGCCGGTAAACAGTATTTGGGCAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGGTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTTGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATcg           <  2:499627/150‑1 (MQ=255)
                                                                                                                                           ccGGGAGATGCCGGTAAACAGTAATTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATcg           >  2:899406/1‑150 (MQ=255)
                                                                                                                                              ggAGATGCCTGTAAACAGTAATTGGGAAGGTTCCGGCAATAATATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATcgcgc        <  2:746294/150‑1 (MQ=255)
                                                                                                                                                agaTGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCgg      <  2:168102/150‑1 (MQ=255)
                                                                                                                                                 gaTGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCTTACTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGt     >  1:300945/1‑150 (MQ=255)
                                                                                                                                                 gaTGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGt     >  2:391982/1‑150 (MQ=255)
                                                                                                                                                  ttGCCGGTAAACAGTATTTGGGAAGGTTGCGGCAATATTATCTGCCGGGATGTGTTGCGCGTGCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGtt    <  1:1292938/149‑1 (MQ=255)
                                                                                                                                                    gCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTtcg  >  1:877827/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCG  >  NC_000913/4415380‑4415677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: