Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,448,424 T→A 5.4% intergenic (+189/‑23) ytfP → / → chpS GGCT‑like protein/antitoxin of the ChpBS toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,448,4240TA 5.4% 380.3 / 7.5 127intergenic (+189/‑23)ytfP/chpSGGCT‑like protein/antitoxin of the ChpBS toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base T (60/60);  new base A (4/3);  total (64/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

Only 100 of 129 total aligned reads displayed.

CGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAAC  >  NC_000913/4448275‑4448567
                                                                                                                                                     |                                                                                                                                               
cGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCTCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACtt                                                                                                                                                 <  1:52131/150‑1 (MQ=255)
 gagaCGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACttt                                                                                                                                                <  2:1623027/150‑1 (MQ=255)
    acgacTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGATGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGta                                                                                                                                             >  1:275625/1‑150 (MQ=255)
     cgacTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGtat                                                                                                                                            >  1:891969/1‑150 (MQ=255)
     cgacTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGtat                                                                                                                                            >  2:254151/1‑150 (MQ=255)
        cTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAAc                                                                                                                                         <  1:254151/150‑1 (MQ=255)
          cGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACtt                                                                                                                                       <  1:92269/150‑1 (MQ=255)
          cGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACtt                                                                                                                                       >  2:1388316/1‑150 (MQ=255)
              ttCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACAAAGTATAACtcacgg                                                                                                                                   <  1:1816475/150‑6 (MQ=255)
              ttCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACAAAGTATAACtcacgg                                                                                                                                   <  2:1281667/150‑6 (MQ=255)
               tCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAagga                                                                                                                                  <  1:450950/150‑1 (MQ=255)
                cTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAACACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGATTTATACTTTGTATAACTTAaggag                                                                                                                                 >  2:1089607/1‑150 (MQ=255)
                 tGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAaggagg                                                                                                                                <  1:333032/150‑1 (MQ=255)
                  gTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCGTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGt                                                                                                                               >  1:1788856/1‑150 (MQ=255)
                  gTTTTGTAAGTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAACCTCTGCTGGCATTCACAAAAGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGt                                                                                                                               >  2:1012030/1‑150 (MQ=255)
                   ttttGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTg                                                                                                                              >  1:1384543/1‑150 (MQ=255)
                      tGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAg                                                                                                                           <  2:379448/150‑1 (MQ=255)
                       gTAATTCCCTCACCATTTGCTTTTCTCTCCGCGCCGCTTTGCATATCGATTAACCCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAACCGTTTCCTGTAGCACCGTGAGTTTTACTTTGTATAACTTAAGGAGGTGCAGa                                                                                                                          >  2:845205/1‑150 (MQ=255)
                        tAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGAt                                                                                                                         <  2:1497603/150‑1 (MQ=255)
                        tAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGAGCAGGGGTAAAACGTTTCCTGAAGCCCCGTGAGATATACGTTGTATAACTTAAGGAGGTGCAGAt                                                                                                                         >  1:285896/1‑150 (MQ=255)
                         aaTTCCCTCCCCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATg                                                                                                                        >  1:1625655/1‑150 (MQ=255)
                             cccTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGGGCAGATGCGTa                                                                                                                    <  1:1132056/150‑1 (MQ=255)
                              ccTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTAt                                                                                                                   >  1:46294/1‑150 (MQ=255)
                                 cACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTAc                                                                                                                <  1:48501/150‑1 (MQ=255)
                                 cACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTAc                                                                                                                <  1:126457/150‑1 (MQ=255)
                                 cACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTAc                                                                                                                <  1:1595043/150‑1 (MQ=255)
                                 cACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTCCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGAATAACTAAAGGAGGTGCAGATGCGTATTAc                                                                                                                >  1:1186536/1‑150 (MQ=255)
                                  aCCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTAcc                                                                                                               <  1:779226/150‑1 (MQ=255)
                                  aCCTTTAGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTAcc                                                                                                               <  1:1744915/150‑1 (MQ=255)
                                    cTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCAt                                                                                                             <  1:1490552/150‑1 (MQ=255)
                                          cTTTTCTCTCCGAGCCGATTTCCATATCTATTAACGCATAAAAAACTCTCCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTCTAGCACCGTGAGTTATACTTTGTAAAACTTAAGGAGGTGCAGATGCGTATAACCAGAAAAAg                                                                                                       >  1:285995/1‑150 (MQ=255)
                                                tctcCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTGTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATgggg                                                                                                 <  2:280472/150‑1 (MQ=255)
                                                 ctcCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAAAGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGAtggtgg                                                                                                >  1:948145/1‑150 (MQ=255)
                                                   ccGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGaa                                                                                              >  1:1167225/1‑150 (MQ=255)
                                                   ccGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGAATTCACAAATTAGCAGGGGTAAAACGTGTCCTGTAGCACCGTGAGTTATACTTAGTATAACTTAAGGAGGTGCAGATGCGTATTACAATAAAAAGATGGGGGaa                                                                                              >  2:305786/1‑150 (MQ=255)
                                                     gAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACa                                                                                            >  1:653676/1‑150 (MQ=255)
                                                      aGCCTCTTTCCAAATCTAATAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTTAGTTATACTTTGTATAACTTAAGGAGGTGCAGATTCGTATTACCATAAAAAGATGGGGGAACAg                                                                                           <  1:1839858/150‑1 (MQ=255)
                                                      aGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAg                                                                                           <  1:1847115/150‑1 (MQ=255)
                                                       gCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGt                                                                                          >  1:643609/1‑150 (MQ=255)
                                                           cTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAg                                                                                      <  1:1567945/150‑1 (MQ=255)
                                                            tttCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAgg                                                                                     <  2:457890/150‑1 (MQ=255)
                                                              tCCATATCTATTAACGCATAAAAAACTCTGCTGGTATTCACAAATGCGCAGGGGTAAAACGTTTCATGTAGCACCGTGAGTTATAATTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTa                                                                                   >  1:917247/1‑150 (MQ=255)
                                                               ccATATCTATTAACGAATAAAAAACTCTGCTGGCATTCACAAAAGCGCAGGGGTAAAACGTTTCGTGTAGCGCCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGAGTATTACAATAAAAAGATTGTGGAACTGTGCAGGTAt                                                                                  >  2:1811894/1‑150 (MQ=255)
                                                                    tCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCa                                                                             >  2:1423948/1‑150 (MQ=255)
                                                                    tCTATTAACGCATAAAAAACTCTCCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCa                                                                             <  1:220014/150‑1 (MQ=255)
                                                                       aTTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTc                                                                          >  1:363724/1‑150 (MQ=255)
                                                                        ttAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTcc                                                                         <  2:448329/150‑1 (MQ=255)
                                                                          aaCGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCa                                                                       >  2:769552/1‑150 (MQ=255)
                                                                             gCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAata                                                                    >  1:1136559/1‑150 (MQ=255)
                                                                              cATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAatat                                                                   >  2:1465880/1‑150 (MQ=255)
                                                                              cATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAAATTTAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAatat                                                                   >  2:861167/1‑150 (MQ=255)
                                                                                  aaaaaCTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTa                                                               >  2:1771418/1‑150 (MQ=255)
                                                                                    aaaCTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAAt                                                             <  2:1659662/150‑1 (MQ=255)
                                                                                         ctgctgGCATTCACAACTGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAg                                                        <  2:311904/150‑1 (MQ=255)
                                                                                         ctgctgGCATTCACAAATGCGCAGGGGTACAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCCGGTATGGTCATTCCCAATATCGTAATGAAAg                                                        <  2:948295/150‑1 (MQ=255)
                                                                                         ctgatgGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAg                                                        <  2:807660/150‑1 (MQ=255)
                                                                                            ctgGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGaac                                                     <  2:1177773/150‑1 (MQ=255)
                                                                                            ctgGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGaac                                                     >  2:1354558/1‑150 (MQ=255)
                                                                                              ggCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGaactt                                                   >  1:1500464/1‑150 (MQ=255)
                                                                                                cATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGTGGAACAGTGCAGGTATGGTCATTCCCAATATATTAATGAAATaaattaa                                                 >  1:334867/1‑150 (MQ=255)
                                                                                                 aTTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGaacttaac                                                <  1:1388316/150‑1 (MQ=255)
                                                                                                    cacaAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAacttaactta                                             >  1:1779433/1‑150 (MQ=255)
                                                                                                     acaAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGTGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTAc                                            <  2:1431146/150‑1 (MQ=255)
                                                                                                        aaTGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGc                                         >  1:1380729/1‑150 (MQ=255)
                                                                                                          tGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCg                                       >  1:1340255/1‑150 (MQ=255)
                                                                                                            cgcAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATCCTTTGTTTAACTTAAGGAGGGGCAGATGCGTATTAGGATAAAAAGATGGGGGGACAGTGCAGGTATGGTCATTCCCAATATCGTATTGAGAGATCTTACCTTACAGCCggg                                     >  2:1096702/1‑150 (MQ=255)
                                                                                                            cgcAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCggg                                     <  2:1136559/150‑1 (MQ=255)
                                                                                                            cgcAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCggg                                     >  2:642050/1‑150 (MQ=255)
                                                                                                              cAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGc                                   <  2:1482409/150‑1 (MQ=255)
                                                                                                              cAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGc                                   <  2:356162/150‑1 (MQ=255)
                                                                                                              cAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGc                                   <  1:1264779/150‑1 (MQ=255)
                                                                                                               aGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCa                                  <  1:1569267/150‑1 (MQ=255)
                                                                                                                  ggTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCagag                               <  2:46294/150‑1 (MQ=255)
                                                                                                                     aaaaCGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGt                            <  2:1711896/150‑1 (MQ=255)
                                                                                                                      aaaCGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTg                           <  2:247673/150‑1 (MQ=255)
                                                                                                                      aaaCGATTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTg                           <  2:812560/150‑1 (MQ=255)
                                                                                                                        aCGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGa                         <  2:275625/150‑1 (MQ=255)
                                                                                                                        aCGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGa                         <  1:392692/150‑1 (MQ=255)
                                                                                                                         cGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAg                        >  2:1721695/1‑150 (MQ=255)
                                                                                                                          gTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTTTTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAgg                       <  2:1838604/150‑1 (MQ=255)
                                                                                                                           tatCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGgc                      <  2:517902/148‑1 (MQ=255)
                                                                                                                              ccTGTAGCAACGTGAGTTATACTTTTTAAAACTTAAGGAGGGGCAGATGCGTAATACCATAAAAAGATGGGGGAACAGTGCAGGTATGGGCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGGGGAGGCGCa                   <  1:1748646/150‑1 (MQ=255)
                                                                                                                               cTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCaa                  >  1:842471/1‑150 (MQ=255)
                                                                                                                                tGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAg                 >  2:794693/1‑150 (MQ=255)
                                                                                                                                  tAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTg               >  1:838151/1‑150 (MQ=255)
                                                                                                                                  tAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTg               <  1:1804487/150‑1 (MQ=255)
                                                                                                                                   aGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGa              >  1:629021/1‑150 (MQ=255)
                                                                                                                                   aGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGa              <  2:764168/150‑1 (MQ=255)
                                                                                                                                    gCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGTAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAg             <  1:1433054/150‑1 (MQ=255)
                                                                                                                                    gCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTAACATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAATTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAg             <  1:1309441/150‑1 (MQ=255)
                                                                                                                                      tccttaaGTTATACAAAGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAATGATGGGGGAACAGTGAAGGTTTGGTCATTCCCATTATCGTAATGAAAGAAGTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGca           >  1:340131/7‑150 (MQ=255)
                                                                                                                                      tccttaaGTTATACAAAGTATAACTTAAGGAGGTGAAGATGCGTATTACCATAAAAAGATGGGGGTACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTAAATCCGGGGCAGAGAGTTGAGGCTCAAGTGACca           >  1:169998/7‑150 (MQ=255)
                                                                                                                                      aCCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGca           >  1:521109/1‑150 (MQ=255)
                                                                                                                                       ccGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGGATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGGGGAGGCGCAAGTGAGcaa          <  2:77620/150‑1 (MQ=255)
                                                                                                                                        cttaaGTTATACAAAGTATAACTTAAGGAGTTGCAGATGCGTATTACAATAAAAAGATGGGTTAACAGTGCAGTTATGGTCATTCCCAATATCGTAAAGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGcaac         >  2:37306/5‑150 (MQ=255)
                                                                                                                                        cGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGcaac         <  2:774980/150‑1 (MQ=255)
                                                                                                                                         gTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGcaaca        <  2:656645/150‑1 (MQ=255)
                                                                                                                                          tGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGcaacaa       <  2:682262/150‑1 (MQ=255)
                                                                                                                                             gTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCa    >  2:1000070/1‑150 (MQ=255)
                                                                                                                                               tataCTTTGTATAACTTAAGGAGGTGAAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAAc  <  2:154974/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
CGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAAC  >  NC_000913/4448275‑4448567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: